Combining Ability Analyses of Net Photosynthesis Rate in Pepper (Capsicum annuum L.)

In perspective of breeding high-yield hybrid pepper varieties, combining ability analysis of net photosynthesis rate at different phases of flowering and fruit setting in pepper was made with 15 cross combinations from 6 parents by （1/2） n （n- 1） diallel crosses. There are relatively large differences not only in general combining ability （GCA） effect among different parents and at different phases of flowering and fruit setting, but also in specific combining ability （SCA） effect among different hybrids. There are relatively large GCA effects in late parents but relatively less GCA effects in early parents. No obvious laws have been found in the relationship between SCA effects and maturity of hybrids. Variances of SCA are larger than those of GCA. Heritability is less but influence of environment is larger. Correlation analysis of combining ability between net photosynthesis rate and agronomic character or resistances to main diseases has showed that correlation coefficients of GCA are relatively large at the medium phase and the late phase of flowering and fruit setting. Net photosynthesis rate is more relative to leaf characters and fruit characters. Correlation coefficients of SCA are relatively large at the early phase and the late phase of flowering and fruit setting. Net photosynthesis rate is more relative to leaf characters and plant characters at the early phase but to plant characters and fruit characters at the late phase. Correlation coefficients of SCA between net photosynthesis rate and resistances to main diseases are larger than those of GCA. The combining abilities of net photosynthesis rate at different phases of flowering and fruit setting are positively correlated with those of yield per plant. The combining ability is an important parameter of breeding of high photosynthesis hybrid pepper varieties.

SIMPLE SIMULATION OF THE ANNUAL VARIATION OF THE SPECIFIC PHOTOSYNTHESIS RATE IN JIAOZHOU BAY

A simple diagnostic simulation of the annual cycling of the surface specific photosynthesis rate (SPR) in Jiaozhou Bay is described in this paper. Light intensity, temperature and nutrients (nitrate + ammonia, phosphate) were considered as main factors controlling photosynthesis of phytoplankton and were introduced into the model by different function equations. The simulated variation of specific photosynthesis rate coincided with the measured data. Analysis of the effect of every factor on photosynthesis indicated that the variation of photosynthesis rate was controlled by all these three factors, while temperature showed good correlation with SPR as measurement showed. This diagnostic simulation yielded the values of some parameter relating with the photosynthesis in Jiaozhou Bay.

Canopy Net Photosynthesis Rate of a Mongolian Oak (<i>Quercus mongolica</i>) Forest Estimated by Field Experimental Data

The canopy net photosynthesis rate of Mongolian oak (Quercus mongolica) tree species that are dominant in East Asia and Korea is estimated with empirical models derived from field experimental data obtained from the Nam-San site in Seoul, Korea for the growing period from early May to late October in 2010. The empirical models include the attenuation function of photosynthetic photon flux density (PPFD) (r2 = 0.98-0.99, p r2 = 0.99, p < 0.001) derived from the measured data at several levels within the canopy. The incident PPFD at each level within canopy significantly varies diurnally and seasonally due to the seasonal variation of the total plant area index (TPAI = leaf area index + wood silhouette area index) and the light shielding effect of light path-length through the canopy in association with the variation of solar elevation angle. Consequently, a remarkable seasonal variation of the total canopy net photosynthesis rate of Q. mongolica forest stand is found for its growing period. The PPFD exceeding 1000 μmol m-2·s-1 is found to cause the decrease of net photosynthesis rate due to the thermal stress in the early (May) and late (September) growing period. During the whole growing season, the estimated total canopy net photosynthesis rate is found to be about 3.3 kg CO2 m-2.

Effect of temperature,salinity and irradiance on growth and photosynthesis of Ulva prolifera

Intensive Pyropia aquaculture in the coast of southwestern Yellow Sea and its subsequent waste, including disposed Ulva prolifera, was speculated to be one of the major sources for the large-scale green tide proceeding in the Yellow Sea since 2007. It was, however, unclear how the detached U. prolifera responded and resumed growing after they detached from its original habitat. In this study, we investigated the growth and photosynthetic response of the detached U. prolifera to various temperature, salinity and irradiance in the laboratory. The photosynthetic rate of the detached U. prolifera was significantly higher at moderate temperature levels（14–27℃）and high salinity（26–32）, with optimum at 23℃ and 32. Both low（14℃） and highest temperature（40℃）, as well as low salinity（8） had adverse effects on the photosynthesis. Compared with the other Ulva species, U. prolifera showed higher saturated irradiance and no significant photoinhibition at high irradiance, indicating the great tolerance of U. prolifera to the high irradiance. The dense branch and complex structure of floating mats could help protect the thalli and reduce photoinhibition in field. Furthermore, temperature exerted a stronger influence on the growth rate of the detached U. prolifera compared to salinity. Overall, the high growth rate of this detached U. prolifera（10.6%–16.7% d^–1） at a wide range of temperature（5–32℃） and salinity（14–32） implied its blooming tendency with fluctuated salinity and temperature during floating. The environmental parameters in the southwestern Yellow Sea at the beginning of green tide were coincident with the optimal conditions for the detached U. prolifera.

Photosynthesis,Metabolite Composition and Anatomical Structure of Oryza sativa and Two Wild Relatives,O.grandiglumis and O.alta

Photosynthesis, enzyme activities and metabolite pools associated with primary carbon metabolism in leaves were studied in O. grandiglumis and O. alta （wild relatives of rice which produce high biomass） versus O. sativa （a japonica cultivar and a indica-japonica hybrid） to assess their potential for identifying traits which might be utilized to enhance rice productivity. The wild relatives had higher rates of photosynthesis on a fresh weight basis, and higher water use efficiency than the O. sativa lines. There were no striking differences in activities of a number of key enzymes in carbon and nitrogen metabolism between the wild relatives and cultivated rice lines. Along with higher rates of photosynthesis on a fresh weight basis, the leaves of the two wild species had higher nitrate content, higher levels of starch, glucose and fructose, and higher levels of organic acids （malate, succinate and acetate）, compared to the O. sativa lines. The results suggested that O. grandiglumis and O. alta have differences in physiology and primary metabolism which might be exploited to improve growth and productivity of cultivated rice.

Research Progress and Prospect on the Effects of Abiotic Stress on Plant Photosynthesis

Photosynthesis is the basis of plant growth and development as well as the existence of the biological world. Photosynthesis is of great theoretical and practical significance. In this paper, the effects of temperature, drought, salt, light and other abiotic stress factors on plant photosynthesis were reviewed.

Research Progress and Prospect on the Influencing Factors of Photosynthesis in Higher Plants

Photosynthesis plays an important role in organic synthesis, solar energy storage, and environmental protection. Photosynthesis is of great theoretical and practical significance. In this paper, the main factors affecting photosynthesis of higher plants, including light, water, temperature, CO2, mineral elements and plant genotypes, were briefly reviewed and summarized, and the development prospect of photosynthesis was prospected.

Photosynthesis of Submerged and Surface Leaves of the Dwarf Water Lily(Nymphoides aquatica)Using PAM Fluorometry

Dwarf Water Lilies Nymphoides aquatica(J.F.Gmel)Kuntze have floating and submerged leaves.Some submerged aquatic vascular plants have a form of CAM(Crassulacean Acid Metabolism)called Submerged Aquatic Macrophyte(SAM)metabolism.Blue-diode based PAM technology was used to measure the Photosynthetic Oxygen Evolution Rate(POER:1O_(2)≡4e^(-)).Optimum Irradiance(E_(opt)),maximum POER(POER_(max))and quantum efficiency(α_(0))all vary on a diurnal cycle.The shape of the POER vs.E curves is different in seedling,submerged and surface leaves.Both E_(opt) and POER_(max) are very low in seedling leaves(E_(opt)≈104μmol photon m^(-2) s^(-1),PPFD;POER_(max)≈4.95µmol O_(2) g^(-1) Chl a s^(-1)),intermediate in mature submerged leaves(E_(opt)≈419µmol photon m^(-2) s^(-1) PPFD,POER_(max)≈38.1µmol O_(2) g^(-1) Chl a s^(-1))and very high in surface leaves(E_(opt)≈923µmol photon m^(-2) s^(-1) PPFD,POER_(max)≈76.1µmol O_(2) g^(-1) Chl a s^(-1)).Leaf titratable acid(C4 acid pool)is too small(≈20 to 50 mol H+m^(-3))to support substantial SAM metabolism.Gross daily photosynthesis of surface leaves is≈3.71 g C m^(-2) d^(-1) in full sun and as much as 1.4 gC m^(-2) d^(-1) in shaded submerged leaves.There is midday inhibition of photosynthesis.

不同氮肥处理对香稻和非香稻品种籽粒灌浆和叶片光合特性的影响

在大田条件下,通过对香稻和非香稻品种的籽粒灌浆、叶片光合特性进行研究,明确香稻和非香稻品种产量和品质形成的生理机制。结果表明:在籽粒灌浆期,相对于非香稻品种,香稻品种在不施氮处理下表现为较高的籽粒灌浆速率,在施氮处理下表现为较低的籽粒灌浆速率;对于剑叶光合速率,无论在施氮还是非施氮条件下均表现为香稻品种高于非香稻品种,说明香稻品种更具有高光效特性。对2品种的产量性状分析表明,香稻品种较非香稻品种粒多,穗大;而非香稻品种相对于香稻品种则穗小,穗数多,这说明,香稻品种主要表现为大穗型,而非香稻品种主要表现为多穗型。对2品种的品质性状进行分析,加工品质2品种差异较小,外观品质表现为非香稻品种明显优于香稻品种,食味值2品种相差相对较小,且均表现为不施氮处理的食味值较高。综合以上分析表明,相对于非香稻品种,香稻品种在不施氮处理下主要表现为较高的籽粒灌浆速率,较高的剑叶光合速率,较高的产量及较高的食味品质;在施氮处理下,除光合速率香稻高于非香稻品种外,其他性状稍低于非香稻品种,这说明,在水稻提优栽培中,在确保产量不降低的情况下,不施或适当少施氮肥,有利于增加香稻品种的籽粒灌浆速率和剑叶光合速率,从而提高香稻的品质。

五个杨树品种生长、光合生理及根尖离子流速特性比较分析

通过比较不同品种杨树(Populus)的生长、光合生理及根尖离子流速特性差异,为速生适应性强品种的早期选育提供参考。以渤丰3号杨(P.×euramericana‘Bofeng 3’)、渤丰1号杨(P.×euramericana‘Bofeng 1’)、西雄1号杨(P.×euramericana‘Xixiong 1’)、中雄7号杨(P.×euramericana‘Zhongxiong 7’)和中雄4号杨(P.deltoides×P.suaveolens cl.‘Zhongxiong 4’)5个杨树品种1年生苗为研究对象,于正常培养条件下的试验开始和试验结束(30 d)时分别测定株高、地径等生长指标,并于10、20、30 d时分别测定幼苗的叶片数量、单叶面积、叶长、叶宽、瞬时净光合速率(P_(n))、胞间CO_(2)摩尔浓度(C_(i))、气孔导度(G_(s))、蒸腾速率(T_(r))、叶绿素相对含量以及根尖K^(+)、Ca^(2+)、H^(+)流速等指标,经过30 d的正常管理,5个品种的株高生长量之间的差异达到显著水平,由高到低依次为中雄4号杨、西雄1号杨、渤丰3号杨、中雄7号杨、渤丰1号杨;其中光合特性指标和蒸腾速率渤丰1号杨均表现为最大,中雄4号杨均表现为最小,但是中雄4号杨的单叶面积((57.49±2.37)cm^(2))、总叶面积((1721.10±28.59)cm^(2))、单株净光合速率((17863.10±910.21)μmol·m-2·s-1)和水分利用率((3.15±0.06)μmol·mmol^(-1))均表现为最大,且K^(+)外排流速最慢,为(62.68±0.45)pmol·cm^(-2)·g^(-1),Ca^(2+)内流流速最快,为(-74.24±1.29)pmol·cm^(-2)·g^(-1),而渤丰1号的单株净光合速率((8539.70±164.64)μmol·m-2·s-1)和水分利用率((2.64±0.07)μmol·mmol^(-1))最小,且K^(+)外排流速((130.81±1.71)pmol·cm^(-2)·g^(-1))最快,Ca^(2+)内流流速((-34.43±0.84)pmol·cm^(-2)·g^(-1))最慢。综上所述,5个杨树品种中,中雄4号杨总叶面积、单株净光合速率、水分利用率最高,蒸腾速率最小,根尖K^(+)外排流速最慢,Ca^(2+)内流流速最快,植物体内K^(+)、Ca^(2+)离子含量最多,H^(+)最活跃,株高生长表现最好,可能具有潜在的较强环境适应能力,适宜种植的范围更广。

CO_2, H_2O exchange and stomatal regulation of regenerated Camptotheca acuminata plantlets during ex vitro acclimatization

For finding the changes in CO2, H20 exchange and their stomatal regulation during ex vitro acclimatization of regenerated Camptotheca acuminata plantlets, the net photosynthesis rate （Pn）, respiration rate （Ro）, light compensation point （Lc） and light saturation point （Ls）, transpiration rate （Tr）, stomatal conductance （gs） and water use efficiency（WUE） were measured during 37 days of ex vitro acclimatization. The results showed that Pn sharply increased until 29 days, then slightly decreased. A substantial decrease in Lc and a substantial increase of Ls in the former two weeks were observed, indicating the light regime enlargement for effective leaf photosynthesis. Tr and gs abruptly decreased during the first week then linearly increased until 29days ex vitro acclimatization, reflecting the strong regulation effect of stomata on water changes of ex vitro acclimating plantlets. Stomatal regulation effect on CO2 exchange was different from that on water exchange, i.e. P, was almost independent of gs during the first week, while P. was significantly correlated with gs thereafter （i.e. dual patterns）. Different from dual patterns of gs-Pn relation, the Tr monotonously linearly increased with gs. Furthermore, WUE was almost independent on gs during the first week, while a marked decreasing tendency with gs was found thereafter. At the beginning of the acclimatization, WUE was mainly determined by photosynthetic capacity, while transpiration becomes a main determinant factor for WUE from 7 to 37 days＇ acclimatization.

6个猕猴桃品种的光合特性分析

为筛选优良猕猴桃品种,以2个泰安市本地主栽品种和4个引进品种为试验材料,测定叶片相关生理指标,使用便携式光合作用测定系统测定6个猕猴桃品种的光合日变化、光合作用光响应(PnPAR),分析净光合速率(Pn)、气孔导度(Gs)、胞间CO_(2)浓度(Ci)、蒸腾速率(Tr)之间的相关性。结果表明,‘徐香’光适应范围为最高,其表观量子效率为0.067,光饱和点为1632μmol/(m^(2)·s),光饱和点与光补偿点差值为1600μmol/(m^(2)·s)。‘泰山1号’净光合速率最大为18.45μmol/(m^(2)·s),光能利用效率最高达到1.26%,均是6个猕猴桃品种中的最高值。综合分析,‘徐香’和‘泰山1号’光合特性最好。以净光合速率、蒸腾速率、气孔导度、胞间CO_(2)浓度作为变量进行相关性分析,发现气孔导度和蒸腾速率对猕猴桃净光合速率的影响呈极显著正相关,胞间CO_(2)浓度和净光合速率呈现显著负相关。

扁核木不同直径茎干的光合生理特征

植物的绿色茎干能在叶片缺失时有效地利用阳光进行光合作用,为植物的生长发育提供额外的能量来源,使其更好地适应极端环境。本研究以分布于西南地区具有绿色茎干的植物——扁核木为对象,比较了扁核木不同直径茎干的光合特性及其与叶绿素含量之间的差异,为扁核木的育种和高效栽培提供依据。结果显示:(1)扁核木茎干具有较强的光合能力,且与叶绿素含量存在显著相关性;(2)不同直径的茎干叶绿素含量和光合能力存在差异,随着扁核木茎干直径的增加,叶绿素含量、净光合速率、光补偿点和光饱和点呈现逐渐下降的趋势,但扁核木不同直径的茎干净光合速率均较高。研究表明:茎干的光合作用功能有助于扁核木在早春无叶期间完成开花的生理过程,提高了其对极端环境的适应性。

自适应判决门限PN码混合捕获方案

提出了一种适用于多径衰落信道下实现快速PN码捕获的自适应判决门限混合捕获方案.系统采用多个非相干相关器进行并行搜索和估计背景功率电平,利用恒虚警方法自适应设置系统的判决门限.通过在整个PN码不定相位上均匀分布各相关器的本地PN码相位,能够避免在多径干扰环境下多个同步相位同时出现在相关器中,消除多径干扰对系统检测性能的影响.文中对检测性能进行了分析和比较,并给出了在均匀背景噪声和多径干扰环境下的仿真结果.结果表明,提出的方法可以有效消除多径干扰的影响.

集雨种植模式下种植密度与行距配置对小麦茎秆糖积累及倒伏性能的影响

【目的】分析沟垄集雨种植模式下不同种植密度和行距配置对小麦茎秆碳水化合物积累和抗倒伏性能的影响,明确适应于小麦沟垄集雨种植合理的密度及行距,为进一步稳定和提升小麦产量提供理论依据。【方法】以北方旱区主栽小麦品种西农979为试验材料,于2019—2021年在集雨种植模式下设置两个密度(低密度:180万株/hm^(2);高密度:225万株/hm^(2))和两种行距处理(等行距:20 cm;非等行距:12.5 cm﹕35 cm﹕12.5 cm),分析不同密度和行距对小麦植株中下部叶片的净光合速率、茎秆非结构性碳水化合物和结构性碳水化合物含量、茎秆折断弯矩和倒伏指数以及籽粒产量的影响。【结果】集雨种植模式下提高种植密度,小麦植株中下部叶片(倒三叶、倒四叶、倒五叶)的净光合速率(Pn)、茎秆基部第二节间非结构性碳水化合物(葡萄糖、果糖、蔗糖)和结构性碳水化合物(半纤维素、纤维素)含量、茎秆折断弯矩均明显降低,茎秆倒伏指数显著增高;而高密度种植条件下非等行距处理可改变植株各指标,其中,相较于高种植密度+等行距处理,植株倒三叶、倒四叶、倒五叶Pn明显提高,增幅分别为7.7%—16.5%、5.3%—37.7%、11.9%—24.9%,茎秆葡萄糖、果糖、蔗糖含量分别提高了9.8%—15.0%、8.8%—27.4%、8.2%—41.1%,半纤维素、纤维素含量分别增加4.5%—19.8%、5.9%—31.2%,茎秆折断弯矩提高4.8%—17.3%,茎秆倒伏指数降低10.9%—25.9%,小麦产量亦显著提高了13.5%—15.2%。相关分析表明,小麦茎秆基部节间葡萄糖、果糖、蔗糖含量与半纤维素、纤维素含量呈极显著正相关;小麦茎秆非结构性碳水化合物、结构性碳水化合物与小麦植株中下部叶片Pn及茎秆折断弯矩呈显著正相关,而与倒伏指数呈极显著负相关。【结论】集雨种植模式下,通过非等行距种植调节群体空间分布,可有效提升小麦植株中下部叶片光合速率,促进茎秆糖类物质合成积累,增强小麦茎秆抗倒伏性能,进而降低小麦倒伏发生率,提高籽粒产量。

Influence of O-O formation pathways and charge transfer mediator on lipid bilayer membrane-like photoanodes for water oxidation

Inspired by the function of crucial components in photosystemⅡ(PSⅡ),electrochemical and dyesensitized photoelectrochemical(DSPEC)water oxidation devices were constructed by the selfassembly of well-designed amphipathic Ru(bda)-based catalysts(bda=2,2'-bipyrdine-6,6'-dicarbonoxyl acid)and aliphatic chain decorated electrode surfaces,forming lipid bilayer membrane(LBM)-like structures.The Ru(bda)catalysts on electrode-supported LBM films demonstrated remarkable water oxidation performance with different O-O formation mechanisms.However,compared to the slow charge transfer process,the O-O formation pathways did not determine the PEC water oxidation efficiency of the dyesensitized photoanodes,and the different reaction rates for similar catalysts with different catalytic paths did not determine the PEC performance of the DSPECs.Instead,charge transfer plays a decisive role in the PEC water oxidation rate.When an indolo[3,2-b]carbazole derivative was introduced between the Ru(bda)catalysts and aliphatic chain-modified photosensitizer in LBM films,serving as a charge transfer mediator for the tyrosine-histidine pair in PSⅡ,the PEC water oxidation performance of the corresponding photoanodes was dramatically enhanced.

不同灌溉方式对西北干旱区紫花苜蓿生产性能的影响

为了明确紫花苜蓿品质形成差异的水分生理特征,探索因地制宜、节水高效的灌溉模式,以河西地区为例,以不灌水(CK)为对照,对比分析了常规畦灌(M)、交替灌溉(T)和隔沟灌溉(G)等3种不同灌溉方式分别在灌底墒水、越冬水、返青水和现蕾水不同灌溉量下对“三得利”紫花苜蓿产量和品质的影响。结果表明:(1)不同灌水方式下株高与灌水次数呈正相关,分支数与灌水量呈负相关;交替灌溉在灌底墒水、越冬水、返青水和现蕾水条件下株高三茬平均值最高为91.92 cm。(2)各灌水模式下,紫花苜蓿净光合速率、蒸腾速率随灌水量的增大表现出增高的趋势,其中交替灌溉的净光合速率随灌水量的增大提高了15.64%,且均高于常规畦灌和隔沟灌溉在同等灌水量下净光合速率。(3)不同处理紫花苜蓿的产量随灌水量的增加而增加,漫灌处理紫花苜蓿的三茬总产量显著高于交替灌溉与隔沟灌溉在相同灌水量下苜蓿产量,在灌底墒水、越冬水、返青水和现蕾水条件下三茬总产最高达49447.6 kg/hm^(2)。(4)不同灌水方式紫花苜蓿粗蛋白含量和中性洗涤纤维含量显著高于无灌水(CK)处理,且随着灌水量的增加有不同程度的提高。河西地区地处干旱和半干旱区,以节水灌溉的角度出发,可以选择交替灌溉灌底墒水、越冬水、返青水和现蕾水的灌溉模式能够在保证高产的同时,达到节水的目的。

施氮量对强筋小麦光合、产量、蛋白质含量和加工品质的影响

为探究冀东地区强筋小麦适宜施氮量,以强筋小麦石优20号和中麦998为材料,通过大田试验设置0 kg·hm^(-2)(N_(0))、180 kg·hm^(-2)(N 180)、210 kg·hm^(-2)(N 210)和240 kg·hm^(-2)(N 240)4个施氮水平,分析了施氮量对强筋小麦光合、产量、蛋白质含量和加工品质的影响。结果表明,与N_(0)处理相比,施氮后石优20号和中麦998的产量均在N 240处理下增加显著,增幅分别为3.20%和13.95%。随施氮量的增加,小麦旗叶叶绿素含量和光合参数均呈先升后降趋势,且在花后15 d达到最大值。施氮对F v/F m影响较小,对ΦPSⅡ影响较明显。旗叶、籽粒内硝酸还原酶和谷氨酰胺合成酶均随施氮量的增加呈先升后降趋势。增施氮肥后籽粒蛋白组分中清蛋白、球蛋白比例下降,醇溶蛋白、谷蛋白比例上升,各组分含量在N 210处理下均最高。面筋含量、沉降值、形成时间和稳定时间均随施氮量的增加先升后降。这说明增施氮肥可改善強筋小麦植株的光合特性,进而提高产量,同时增加植株体内氮代谢相关酶活性和籽粒蛋白质含量并改变蛋白质组分所占比例,有效改善加工品质。本试验条件下,210 kg·hm^(-2)施氮量下,小麦产量和品质较优,该施氮量可作为冀东平原强筋小麦栽培的最佳施氮量。

一种快速同步统计高阶调制下PN码误码率的方法

提出了一种快速同步统计高阶调制方式下PN码误码率的方法,用于实时检测与统计误码率,更加方便、快捷、直观地了解数据链的情况与信道的质量。对于高阶调制方式8PSK/16APSK/16QAM,解调后数据存在相位模糊,先解相位模糊后输出解映射数据,PN码产生模块根据预置的PN码生成多项式和从解映射后的数据中提取的初相产生用来进行误码比对的标准PN码,与解映射后的数据进行比对;统计1000个比特,若误码率大于1e-1,重复上述过程,并且给出一个没有同步的标记输入解模糊状态模块,直至误码率小于1e-1,保持该同步状态,维持当前误码统计过程。该方法能够显著减少PN码同步所需的硬件资源,并且同步时间不受PN码周期影响,便于工程实现。

胃癌患者术后预后的独立危险因素及淋巴结转移率对不同pN分期胃癌患者预后的影响

目的分析胃癌患者术后预后的独立危险因素及淋巴结转移率对不同pN分期胃癌患者预后的影响。方法随机选取2014年1月至2015年10月在湖州市中心医院进行手术切除治疗的胃癌患者100例作为研究对象。采用电话、门诊等方法进行随访,随访时间超过60个月。比较100例胃癌患者的临床基本资料,采用Log-Rank检验分析100例胃癌患者60个月生存率变化。采用Kaplan-Meier检验分析影响胃癌患者术后预后的单因素。采用Cox比例风险回归模型分析影响胃癌患者术后预后的多因素。采用分层分析淋巴结转移率对不同pN分期胃癌患者预后的影响。结果100例胃癌患者中,男性比例明显高于女性,年龄大多超过55岁,肿瘤直径<4 cm,肿瘤部位以胃下多见,pT4分期占比明显高于pT1、p T2、pT3,pN0、pN1、pN2、pN3分期占比分别为32.00%、20.00%、22.00%、26.00%;淋巴结转移率,无转移、≤20%、≥21%占比分别为32.00%、18.00%、50.00%,患者CA19-9、CEA含量分别以低于27 U/ml、5μg/L多见,血小板计数在(100~300)×10^(9)个/L,胃癌大多数为低分化,且患者术后经化疗治疗。100例胃癌患者中,术后12、36、60个月生存率分别为80.00%、60.00%、52.00%。经Kaplan-Meier检验分析,肿瘤直径、肿瘤部位、pT分期、pN分期、淋巴结转移率、CA19-9、CEA、血小板计数、原发术后化疗、分化程度均为影响胃癌预后的危险因素(P<0.05)。经Cox比例风险回归模型分析,肿瘤直径、pT分期、pN分期、淋巴结转移率、CA19-9、CEA等均为影响胃癌患者术后预后的独立危险因素(P<0.05)。经Kaplan-Meier分析,淋巴结转移率对pN1期及pN2期胃癌患者的60个月生存率具有明显影响(P<0.05)。结论肿瘤直径、pT分期、pN分期、淋巴结转移率、CA19-9、CEA均为胃癌患者术后预后的独立危险因素,淋巴结转移率对pN1、pN2胃癌患者60个月生存率有显著影响。