Neurogenesis from inner brain neural stem cells （NSCs） is a process which takes place continuously in mammals through- out their life. However, the main ontogenic difference is the intensity of neurogenesis, which c...Neurogenesis from inner brain neural stem cells （NSCs） is a process which takes place continuously in mammals through- out their life. However, the main ontogenic difference is the intensity of neurogenesis, which commences as a very intensive and global activity in the early embryonic brain （neural tube）, persists in fetal and newborn stages, and declines significantly in adulthood, becoming restricted to specific places with low neurogenic activity such as the subventricular zone （SVZ） and the subgranular zone （SGZ） in the dentate gyrus （DG） of the hippocampus.展开更多
This paper introduces the effects of cell operating temperature, methanol concentration and airflow rate, respectively, on the performance of direct methanol fuel cell （DMFC）. A novel method based on fuzzy neural ne...This paper introduces the effects of cell operating temperature, methanol concentration and airflow rate, respectively, on the performance of direct methanol fuel cell （DMFC）. A novel method based on fuzzy neural networks identification technique is proposed to establish the performance model of DMFC. Three dynamic performance models of DMFC under the influences of cell operating temperature, methanol concentration, and airflow rate are identified and established separately. Simulation results show that modeling using fuzzy neural networks identification is satisfactory with high accuracy. It is applicable to DMFC control systems.展开更多
The mass production of primed electronics can be achieved by roll-to-roll（R2R） printing system, so highly accurate web tension is required that can minimize the register error and keep the thickness and roughness of...The mass production of primed electronics can be achieved by roll-to-roll（R2R） printing system, so highly accurate web tension is required that can minimize the register error and keep the thickness and roughness of printed devices in limits. The web tension of a R2R system is regulated by the use of integrated load cells and active dancer system for printed electronics applications using decentralized multi-input-single-output（MISO） regularized variable learning rate backpropagation artificial neural networks. The active dancer system is used before printing system to reduce disturbances in the web tension of process span. The classical PID control result in tension spikes with the change in roll diameter of winder and unwinder rolls. The presence of dancer in R2R system shows that improved web tension control in printing span and the web tension can be enhanced from 3.75 N to 4.75 N. The overshoot of system is less than ±2.5 N and steady state error is within ± 1 N where load cells have a signal noise of ±0.7 N. The integration of load cells and active dancer with self-adapting neural network control provide a solution to the web tension control of multispan roll-to-roll system.展开更多
This paper illustrates the benefits of a self-tuning PID strategy applied to a proton exchange membrane fuel cell system. Controller parameters are updated on-line, at each sampling time, based on an instantaneous lin...This paper illustrates the benefits of a self-tuning PID strategy applied to a proton exchange membrane fuel cell system. Controller parameters are updated on-line, at each sampling time, based on an instantaneous linearization of an artificial neural network model of the process and a General Minimum Variance control law. The self-tuning PID scheme allows managing nonlinear behaviors of the system while avoiding heavy computations. The applicability, efficiency and robustness of the proposed control strategy are experimentally confirmed using varying control scenarios. In this aim, the original built-in controller is overridden and the self-tuning PID controller is implemented externally and executed on-line. Experimental results show good performance in setpoint tracking accuracy and robustness against plant/model mismatch. The proposed strategy appears to be a promising alternative to heavy computation nonlinear control strategies and not optimal linear control strategies.展开更多
In this study, we prepared PLLA/bpV（pic） microspheres, a bpV（pic） controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing...In this study, we prepared PLLA/bpV（pic） microspheres, a bpV（pic） controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received： a routine medium group（cultured in DMEM）, a PLLA microsphere group（DMEM containing PLLA microspheres alone） and a PLLA/bpV（pic） group [DMEM containing PLLA/bpV（pic） microspheres]. The effects of PLLA/bpV（pic） microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV（pic） granulation rate was（88.2±5.6）%; particle size was（16.8±3.1）%, drug loading was（4.05±0.3）%; encapsulation efficiency was（48.5±1.8）%. The release time lasted for 30 days. In PLLA/bpV（pic） microsphere group, the cell survival rate was（95.2 ±4.77）%, and the length of dorsal root ganglion（DRG） was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV（pic） microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.展开更多
The injury of central nervous system (CNS) usually causes the cavity formation. Although transplantation of neural stem/precursor cells (NSPCs) into the lesioned area of CNS has been shown to be implicated in the func...The injury of central nervous system (CNS) usually causes the cavity formation. Although transplantation of neural stem/precursor cells (NSPCs) into the lesioned area of CNS has been shown to be implicated in the functional restoration, the therapeutic result is limited by the poor survival of NSPCs as well as their insufficient proliferation and differentiation abilities. Type-1 collagen is considered as a candidate scaffold or drug delivery system to overcome the aforementioned obstacle. This study observed the effects of the CNTF (ciliary neurotrophic factor)-collagen gel-controlled delivery system and daily addition of soluble-form CNTF on the NSPC survival, migration, proliferation and differentiation. The results showed that, within 12 h of the initial co-culture, CNTF was released in a burst pattern, then the CNTF-collagen gel-controlled delivery system stably released CNTF for up to 12 d. The cell viability test, together with immunohistochemistry, RT-PCR and Western blotting, showed that the CNTF-collagen gel-controlled delivery system supported the NSPCs seeded on the surface of collagen gel survival and facilitated their migration and proliferation. The daily addition of soluble-form CNTF to the medium had similar effects to the CNTF-collagen gel-controlled delivery system, but large quantities of soluble-form CNTF were consumed during the entire process. Taken together, the CNTF-collagen gel-controlled delivery system not only provides a physical scaffold for the transplanted NSPCs to adhere and migrate, but also facilitates the NSPC survival, growth and proliferation, simultaneously reducing the consumption of the expensive growth factors. This system may be used to enhance the microenvironment in the lesioned area of CNS.展开更多
文摘Neurogenesis from inner brain neural stem cells （NSCs） is a process which takes place continuously in mammals through- out their life. However, the main ontogenic difference is the intensity of neurogenesis, which commences as a very intensive and global activity in the early embryonic brain （neural tube）, persists in fetal and newborn stages, and declines significantly in adulthood, becoming restricted to specific places with low neurogenic activity such as the subventricular zone （SVZ） and the subgranular zone （SGZ） in the dentate gyrus （DG） of the hippocampus.
基金Project supported by the National High-Technology Research and Development Program Foundation of China(Grant No.2003AA517020)
文摘This paper introduces the effects of cell operating temperature, methanol concentration and airflow rate, respectively, on the performance of direct methanol fuel cell （DMFC）. A novel method based on fuzzy neural networks identification technique is proposed to establish the performance model of DMFC. Three dynamic performance models of DMFC under the influences of cell operating temperature, methanol concentration, and airflow rate are identified and established separately. Simulation results show that modeling using fuzzy neural networks identification is satisfactory with high accuracy. It is applicable to DMFC control systems.
基金supported by Basic Science Research Program through the National Research Foundation of Korea(NRF),Ministry of Education,Science and Technology,Korea(Grant No.2010-0026163)Strategy Technology Development Project,Ministry of Knowledge Economy,Korea(Grant No.10032149)
文摘The mass production of primed electronics can be achieved by roll-to-roll（R2R） printing system, so highly accurate web tension is required that can minimize the register error and keep the thickness and roughness of printed devices in limits. The web tension of a R2R system is regulated by the use of integrated load cells and active dancer system for printed electronics applications using decentralized multi-input-single-output（MISO） regularized variable learning rate backpropagation artificial neural networks. The active dancer system is used before printing system to reduce disturbances in the web tension of process span. The classical PID control result in tension spikes with the change in roll diameter of winder and unwinder rolls. The presence of dancer in R2R system shows that improved web tension control in printing span and the web tension can be enhanced from 3.75 N to 4.75 N. The overshoot of system is less than ±2.5 N and steady state error is within ± 1 N where load cells have a signal noise of ±0.7 N. The integration of load cells and active dancer with self-adapting neural network control provide a solution to the web tension control of multispan roll-to-roll system.
文摘This paper illustrates the benefits of a self-tuning PID strategy applied to a proton exchange membrane fuel cell system. Controller parameters are updated on-line, at each sampling time, based on an instantaneous linearization of an artificial neural network model of the process and a General Minimum Variance control law. The self-tuning PID scheme allows managing nonlinear behaviors of the system while avoiding heavy computations. The applicability, efficiency and robustness of the proposed control strategy are experimentally confirmed using varying control scenarios. In this aim, the original built-in controller is overridden and the self-tuning PID controller is implemented externally and executed on-line. Experimental results show good performance in setpoint tracking accuracy and robustness against plant/model mismatch. The proposed strategy appears to be a promising alternative to heavy computation nonlinear control strategies and not optimal linear control strategies.
基金supported by Science and Technology Plan-ning Project of Guangdong Province,China(No.2011B031800205)
文摘In this study, we prepared PLLA/bpV（pic） microspheres, a bpV（pic） controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received： a routine medium group（cultured in DMEM）, a PLLA microsphere group（DMEM containing PLLA microspheres alone） and a PLLA/bpV（pic） group [DMEM containing PLLA/bpV（pic） microspheres]. The effects of PLLA/bpV（pic） microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV（pic） granulation rate was（88.2±5.6）%; particle size was（16.8±3.1）%, drug loading was（4.05±0.3）%; encapsulation efficiency was（48.5±1.8）%. The release time lasted for 30 days. In PLLA/bpV（pic） microsphere group, the cell survival rate was（95.2 ±4.77）%, and the length of dorsal root ganglion（DRG） was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV（pic） microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.
基金supported by the National High Technology Research and Development Program of China (Grant No. 2006AA02A129) the Key Project of Department of Science and Technology of Beijing (Grant No. D09080703660902)
文摘The injury of central nervous system (CNS) usually causes the cavity formation. Although transplantation of neural stem/precursor cells (NSPCs) into the lesioned area of CNS has been shown to be implicated in the functional restoration, the therapeutic result is limited by the poor survival of NSPCs as well as their insufficient proliferation and differentiation abilities. Type-1 collagen is considered as a candidate scaffold or drug delivery system to overcome the aforementioned obstacle. This study observed the effects of the CNTF (ciliary neurotrophic factor)-collagen gel-controlled delivery system and daily addition of soluble-form CNTF on the NSPC survival, migration, proliferation and differentiation. The results showed that, within 12 h of the initial co-culture, CNTF was released in a burst pattern, then the CNTF-collagen gel-controlled delivery system stably released CNTF for up to 12 d. The cell viability test, together with immunohistochemistry, RT-PCR and Western blotting, showed that the CNTF-collagen gel-controlled delivery system supported the NSPCs seeded on the surface of collagen gel survival and facilitated their migration and proliferation. The daily addition of soluble-form CNTF to the medium had similar effects to the CNTF-collagen gel-controlled delivery system, but large quantities of soluble-form CNTF were consumed during the entire process. Taken together, the CNTF-collagen gel-controlled delivery system not only provides a physical scaffold for the transplanted NSPCs to adhere and migrate, but also facilitates the NSPC survival, growth and proliferation, simultaneously reducing the consumption of the expensive growth factors. This system may be used to enhance the microenvironment in the lesioned area of CNS.
