Traumatic brain injury（TBI） is a leading cause of death and disability in individuals worldwide.Producing a clinically relevant TBI model in small-sized animals remains fairly challenging.For good screening of poten...Traumatic brain injury（TBI） is a leading cause of death and disability in individuals worldwide.Producing a clinically relevant TBI model in small-sized animals remains fairly challenging.For good screening of potential therapeutics,which are effective in the treatment of TBI,animal models of TBI should be established and standardized.In this study,we established mouse models of closed head injury using the Shohami weight-drop method with some modifications concerning cognitive deficiency assessment and provided a detailed description of the severe TBI animal model.We found that 250 g falling weight from 2 cm height produced severe closed head injury in C57BL/6 male mice.Cognitive disorders in mice with severe closed head injury could be detected using passive avoidance test on day 7 after injury.Findings from this study indicate that weight-drop injury animal models are suitable for further screening of brain neuroprotectants and potentially are similar to those seen in human TBI.展开更多
目的探讨脑氧摄取率(CERO_(2))、脑氧饱和度(rSO_(2))与重症颅脑损伤神经功能预后不良的关系及其预测神经功能预后的价值。方法回顾性分析2018年1月—2019年6月医院收治的106例重症颅脑损伤术后患者和同时期本院收治的可疑脑损伤但是经...目的探讨脑氧摄取率(CERO_(2))、脑氧饱和度(rSO_(2))与重症颅脑损伤神经功能预后不良的关系及其预测神经功能预后的价值。方法回顾性分析2018年1月—2019年6月医院收治的106例重症颅脑损伤术后患者和同时期本院收治的可疑脑损伤但是经检查健康的96例的资料,分别记为观察组和对照组。对观察组患者随访6个月依据格拉斯哥预后(GOS)评分进行重症颅脑损伤术后神经功能预后评价。对比观察组与对照组脑代谢指标(CERO_(2)、rSO_(2))、观察组中预后良好者与预后不良者术后24 h CERO_(2)、rSO_(2),分析术后24 h CERO_(2)、rSO_(2)与重症颅脑损伤术后神经功能预后不良的关系,分析术后24 h CERO_(2)、rSO_(2)与重症颅脑损伤术后神经功能预后不良的关系及预测价值。结果观察组CERO_(2)、rSO_(2)均低于对照组(P<0.05);重症颅脑损伤术后神经功能预后不良发生率为23.58%;观察组术后神经功能预后不良者术后24 h CERO_(2)、rSO_(2)均低于神经功能预后良好者(P<0.05)。多因素分析显示,年龄、合并基础疾病、开放性颅脑损伤、受伤至手术开始时间、术前格拉斯哥昏迷量表(GCS)评分中特重型、首次开颅手术时间、首次开颅手术中出血量、脑疝形成、颅内感染、康复治疗不依从、术后24 h CERO_(2)和术后24 h rSO_(2)均是重症颅脑损伤术后神经功能预后不良的影响因素(P<0.05);术后24 h CERO_(2)、rSO_(2)联合预测重症颅脑损伤术后神经功能预后不良的灵敏度、曲线下面积(AUC)显著高于单独指标预测(P<0.05)。结论重症颅脑损伤患者术后24 h CERO_(2)、rSO_(2)均偏低,且二者均是重症颅脑损伤术后神经功能预后不良的影响因素,联合预测神经功能预后不良的价值高。展开更多
基金supported by a grant from the Ministry of Higher Education of Malaysia,No.RAGS/2013/UPNM/SKK/01/2
文摘Traumatic brain injury（TBI） is a leading cause of death and disability in individuals worldwide.Producing a clinically relevant TBI model in small-sized animals remains fairly challenging.For good screening of potential therapeutics,which are effective in the treatment of TBI,animal models of TBI should be established and standardized.In this study,we established mouse models of closed head injury using the Shohami weight-drop method with some modifications concerning cognitive deficiency assessment and provided a detailed description of the severe TBI animal model.We found that 250 g falling weight from 2 cm height produced severe closed head injury in C57BL/6 male mice.Cognitive disorders in mice with severe closed head injury could be detected using passive avoidance test on day 7 after injury.Findings from this study indicate that weight-drop injury animal models are suitable for further screening of brain neuroprotectants and potentially are similar to those seen in human TBI.
文摘目的探讨脑氧摄取率(CERO_(2))、脑氧饱和度(rSO_(2))与重症颅脑损伤神经功能预后不良的关系及其预测神经功能预后的价值。方法回顾性分析2018年1月—2019年6月医院收治的106例重症颅脑损伤术后患者和同时期本院收治的可疑脑损伤但是经检查健康的96例的资料,分别记为观察组和对照组。对观察组患者随访6个月依据格拉斯哥预后(GOS)评分进行重症颅脑损伤术后神经功能预后评价。对比观察组与对照组脑代谢指标(CERO_(2)、rSO_(2))、观察组中预后良好者与预后不良者术后24 h CERO_(2)、rSO_(2),分析术后24 h CERO_(2)、rSO_(2)与重症颅脑损伤术后神经功能预后不良的关系,分析术后24 h CERO_(2)、rSO_(2)与重症颅脑损伤术后神经功能预后不良的关系及预测价值。结果观察组CERO_(2)、rSO_(2)均低于对照组(P<0.05);重症颅脑损伤术后神经功能预后不良发生率为23.58%;观察组术后神经功能预后不良者术后24 h CERO_(2)、rSO_(2)均低于神经功能预后良好者(P<0.05)。多因素分析显示,年龄、合并基础疾病、开放性颅脑损伤、受伤至手术开始时间、术前格拉斯哥昏迷量表(GCS)评分中特重型、首次开颅手术时间、首次开颅手术中出血量、脑疝形成、颅内感染、康复治疗不依从、术后24 h CERO_(2)和术后24 h rSO_(2)均是重症颅脑损伤术后神经功能预后不良的影响因素(P<0.05);术后24 h CERO_(2)、rSO_(2)联合预测重症颅脑损伤术后神经功能预后不良的灵敏度、曲线下面积(AUC)显著高于单独指标预测(P<0.05)。结论重症颅脑损伤患者术后24 h CERO_(2)、rSO_(2)均偏低,且二者均是重症颅脑损伤术后神经功能预后不良的影响因素,联合预测神经功能预后不良的价值高。
