OBJECTIVE:To investigate the influence of Qihuang decoction(芪黄煎剂)on enteric nervous system after gastrectomy in rats.METHODS:The morphology,distribution and number of intestinal neurons in enteric nervous system(E...OBJECTIVE:To investigate the influence of Qihuang decoction(芪黄煎剂)on enteric nervous system after gastrectomy in rats.METHODS:The morphology,distribution and number of intestinal neurons in enteric nervous system(ENS)were observed by immunofluorescence labeling and confocal laser scanning microscopy.Reverse transcriptionpolymerase chain reaction and Western blot were used to detect the mRNA and protein expression of intestinal neurotransmitters and corresponding receptors in ENS.RESULTS:The morphology and distribution of enteric neurons in ENS were changed after gastrectomy,and these neurons in Qihuang decoction group were similar with that of sham operation group.The number of ACh and SP positive neurons,mRNA and protein expression of excitatory neurotransmitters(ACh E,SP)and receptors(M3R,NK1R)were decreased after gastrectomy.And the intervention of Qihuang decoction could increase the number of ACh and SP positive neurons and promote the expression of their mRNA and protein.For vasoactive intestinal peptide(VIP)and nitric oxide synthase(NOS),the number of neurons and mRNA and protein expression of inhibitory neurotransmitters(VIP and NOS)and receptors(VIP2R)were increased after gastrectomy.And these rising indexes fall back after the intervention of Qihuang decoction.Besides,the intestinal propulsion rate in QH group was significantly increased than that in SEN and IEN group.CONCLUSIONS:These experimental results showed that after gastrectomy,early intervention with Qihuang decoction in small intestine will contribute to the postoperative recovery of enteric nervous system and intestinal propulsion rate,and consequently enhance gastrointestinal motility.展开更多
基金Supported by Clinical Scientific Research Project of the First Affiliated Hospital of Anhui University of Chinese Medicine:to Investigate the Effect of Qihuang Decoction on Gastrointestinal Function and its Molecular Mechanism in Rats after Gastrectomy based on ENS-ICC-SMC Network Architecture(2020yfyzc02)the National Natural Science Foundation of China:the Effect of Qihuang Decoction on Intestinal Lymphocyte Homing after Gastrectomy in Rats(No.81403406),the National Natural Science Foundation of China:Qihuang Decoction Inhibits Bacterial Migration during Gastrectomy in Rats and Regulates the M1-type Polarization of Macrophages induced by TLR4/NF-κB Signaling Pathway based on lncRNA MALAT1 Sponge Mir-146a(No.82174160)+1 种基金Supporting Program for Excellent Young Talents in Colleges and Universities of Anhui Province:the Effects of Qihuang Decoction on Gastrointestinal Motility in Rats after Gastrectomy and its Molecular Mechanism were Studied based on ENS Framework(gxyq2019035)General projects of Anhui Natural Science Foundation:to Investigate the Inhibition of Cryptotanshinone on 5-FUSGC-7901 Human Gastric Cancer Cells and its Mechanism based on JAK2/STAT3 Signaling Pathway(2008085MH266)
文摘OBJECTIVE:To investigate the influence of Qihuang decoction(芪黄煎剂)on enteric nervous system after gastrectomy in rats.METHODS:The morphology,distribution and number of intestinal neurons in enteric nervous system(ENS)were observed by immunofluorescence labeling and confocal laser scanning microscopy.Reverse transcriptionpolymerase chain reaction and Western blot were used to detect the mRNA and protein expression of intestinal neurotransmitters and corresponding receptors in ENS.RESULTS:The morphology and distribution of enteric neurons in ENS were changed after gastrectomy,and these neurons in Qihuang decoction group were similar with that of sham operation group.The number of ACh and SP positive neurons,mRNA and protein expression of excitatory neurotransmitters(ACh E,SP)and receptors(M3R,NK1R)were decreased after gastrectomy.And the intervention of Qihuang decoction could increase the number of ACh and SP positive neurons and promote the expression of their mRNA and protein.For vasoactive intestinal peptide(VIP)and nitric oxide synthase(NOS),the number of neurons and mRNA and protein expression of inhibitory neurotransmitters(VIP and NOS)and receptors(VIP2R)were increased after gastrectomy.And these rising indexes fall back after the intervention of Qihuang decoction.Besides,the intestinal propulsion rate in QH group was significantly increased than that in SEN and IEN group.CONCLUSIONS:These experimental results showed that after gastrectomy,early intervention with Qihuang decoction in small intestine will contribute to the postoperative recovery of enteric nervous system and intestinal propulsion rate,and consequently enhance gastrointestinal motility.
