In recent years, tumor-nfiltrating lymphocytes (TILs) have been reported to be effective for tumors in experimental and clinical research. In order to increase the therapeutical effect, we modified some steps of Rosen...In recent years, tumor-nfiltrating lymphocytes (TILs) have been reported to be effective for tumors in experimental and clinical research. In order to increase the therapeutical effect, we modified some steps of Rosenberg's approach a. cold digestion with collagenase at 4C for 24 hours; b. sedimentation instead of centrifugation; c. elimination of tumor cells before the cultivation procedure. Compared with the original approach, the proliferation, activity and cytotoxicity of TILs obtained by the modified procedure were much improved. TILs' expansion-old was greater than that with the original approach. Cytotoxicity against rumor cells was more potent. Increased TILs' subsets were CD3 and CD8 cells. Meanwhile, we took tumor cells from tumor tissues to test their in vitro chemosensitivities to different drugs in order to select highly sensitive antitumor drugs for treatment of cases with advanced tumors. According to the design of using highly active TILs and highly sensitive drugs (H & H therapy), preliminary clinical results of 50 cases showed higher response rates than those in treatment with TIL / IL2, LAK / 1L2 and TIL+IL2+CTX. Less toxic side effects were observed in 14 patients.展开更多
The paper tends to make an empirical analysis on the experimental site of new countryside construction of Xianhong in Hubei, pointing out that the inner and outer dependence on routines of experimental construction. T...The paper tends to make an empirical analysis on the experimental site of new countryside construction of Xianhong in Hubei, pointing out that the inner and outer dependence on routines of experimental construction. The paper also puts forward the beneficial policies, focusing on the agricultural production and fostering professional farmers, to reduce much dependence on routines.展开更多
Mr.Liu Zhenya,general manager of the State Grid and Mr.Fu Zhifang,executive vice governor of Hebei Province together with their colleagues jointly inspected the development of new energy project in Zhangbei County,Zha...Mr.Liu Zhenya,general manager of the State Grid and Mr.Fu Zhifang,executive vice governor of Hebei Province together with their colleagues jointly inspected the development of new energy project in Zhangbei County,Zhangjiakou City,Hebei Province.展开更多
从昆虫智能识别入手开展新农科背景下昆虫学的实验教学改革和实践。软件由昆虫图片上传、模型训练、目标检测以及导出结果4个模块组成。完成目标检测的图片根据需要保存。AI昆虫识别软件的开发过程在Visual Studio Code环境下进行,采用P...从昆虫智能识别入手开展新农科背景下昆虫学的实验教学改革和实践。软件由昆虫图片上传、模型训练、目标检测以及导出结果4个模块组成。完成目标检测的图片根据需要保存。AI昆虫识别软件的开发过程在Visual Studio Code环境下进行,采用Python语言进行编程,实现对拍摄图片的高效率、高精度检测。最后拟通过学生本科实验教学环节,开放成为农科学生进行昆虫识别鉴定的实践工具,极大激发农科学生的上课激情,同时也有助于在新农科背景下培养农科类学生的现代农业创新思维。展开更多
文摘In recent years, tumor-nfiltrating lymphocytes (TILs) have been reported to be effective for tumors in experimental and clinical research. In order to increase the therapeutical effect, we modified some steps of Rosenberg's approach a. cold digestion with collagenase at 4C for 24 hours; b. sedimentation instead of centrifugation; c. elimination of tumor cells before the cultivation procedure. Compared with the original approach, the proliferation, activity and cytotoxicity of TILs obtained by the modified procedure were much improved. TILs' expansion-old was greater than that with the original approach. Cytotoxicity against rumor cells was more potent. Increased TILs' subsets were CD3 and CD8 cells. Meanwhile, we took tumor cells from tumor tissues to test their in vitro chemosensitivities to different drugs in order to select highly sensitive antitumor drugs for treatment of cases with advanced tumors. According to the design of using highly active TILs and highly sensitive drugs (H & H therapy), preliminary clinical results of 50 cases showed higher response rates than those in treatment with TIL / IL2, LAK / 1L2 and TIL+IL2+CTX. Less toxic side effects were observed in 14 patients.
基金Supported by the Social and Scientific Funds Projects of Hubei Province in 2009 (203004)
文摘The paper tends to make an empirical analysis on the experimental site of new countryside construction of Xianhong in Hubei, pointing out that the inner and outer dependence on routines of experimental construction. The paper also puts forward the beneficial policies, focusing on the agricultural production and fostering professional farmers, to reduce much dependence on routines.
文摘Mr.Liu Zhenya,general manager of the State Grid and Mr.Fu Zhifang,executive vice governor of Hebei Province together with their colleagues jointly inspected the development of new energy project in Zhangbei County,Zhangjiakou City,Hebei Province.
文摘从昆虫智能识别入手开展新农科背景下昆虫学的实验教学改革和实践。软件由昆虫图片上传、模型训练、目标检测以及导出结果4个模块组成。完成目标检测的图片根据需要保存。AI昆虫识别软件的开发过程在Visual Studio Code环境下进行,采用Python语言进行编程,实现对拍摄图片的高效率、高精度检测。最后拟通过学生本科实验教学环节,开放成为农科学生进行昆虫识别鉴定的实践工具,极大激发农科学生的上课激情,同时也有助于在新农科背景下培养农科类学生的现代农业创新思维。
