Renal fibrogenesis is related to the development of diabetic nephropathy.TGF-b receptor II(TGF-b RII)plays a vital role during renal fibrogenesis by phosphorylation and activation of type I receptors and downstream re...Renal fibrogenesis is related to the development of diabetic nephropathy.TGF-b receptor II(TGF-b RII)plays a vital role during renal fibrogenesis by phosphorylation and activation of type I receptors and downstream regulators.Nicousamide is a 1.1 class of drug,which can inhibit renal fibrosis in animal models of diabetic nephropathy.After cloning and purification of TGF-b RII,an in vitro substrate phosphorylation assay was set up to investigate the ability of nicousamide to inhibit phosphorylation and formation of cellular downstream proteins.Our results validate nicousamide as a potent inhibitor of phosphorylation of TGF-b RII.展开更多
基金supported by the National Natural Science Foundation Program(Grant No.30672493).
文摘Renal fibrogenesis is related to the development of diabetic nephropathy.TGF-b receptor II(TGF-b RII)plays a vital role during renal fibrogenesis by phosphorylation and activation of type I receptors and downstream regulators.Nicousamide is a 1.1 class of drug,which can inhibit renal fibrosis in animal models of diabetic nephropathy.After cloning and purification of TGF-b RII,an in vitro substrate phosphorylation assay was set up to investigate the ability of nicousamide to inhibit phosphorylation and formation of cellular downstream proteins.Our results validate nicousamide as a potent inhibitor of phosphorylation of TGF-b RII.
