Basement membrane proteins are known to guide cell structures,differentiation,and tissue repair.Although there is a wealth of knowledge on the functions of laminins,per-lecan,and type IV collagen in maintaining tissue...Basement membrane proteins are known to guide cell structures,differentiation,and tissue repair.Although there is a wealth of knowledge on the functions of laminins,per-lecan,and type IV collagen in maintaining tissue homeostasis,not much is known about nidogen.As a key molecule in the basement membrane,nidogen contributes to the formation of a delicate microenvironment that proves necessary for stem cell lineage-specific differentiation.In this review,the expression of nidogen is delineated at both cellular and tissue levels from embryonic to adult stages of development;the effect of nidogens is also summarized in the context of musculoskeletal development and regeneration,including but not limited to adipogenesis,angiogenesis,chondrogenesis,myogenesis,and neurogenesis.Furthermore,potential mechanisms underlying the role of nidogens in stem cell-based tissue regeneration are also discussed.This concise review is expected to facilitate our existing understanding and utilization of nidogen in tissue engineering and regeneration.展开更多
The technique bottleneck of repairing large bone defects with tissue engineered bone is the vascularization of tissue engineered grafts.Although some studies have shown that extracellular vesicles(EVs)derived from bon...The technique bottleneck of repairing large bone defects with tissue engineered bone is the vascularization of tissue engineered grafts.Although some studies have shown that extracellular vesicles(EVs)derived from bone marrow mesenchymal stem cells(BMSCs)promote bone healing and repair by accelerating angiogenesis,the effector molecules and the mechanism remain unclear,which fail to provide ideas for the future research and development of cell-free interventions.Here,we found that Nidogen1-enriched EV(EV-NID1)derived from BMSCs interferes with the formation and assembly of focal adhesions(FAs)by targeting myosin-10,thereby reducing the adhesion strength of rat arterial endothelial cells(RAECs)to the extracellular matrix(ECM),and enhancing the migration and angiogenesis potential of RAECs.Moreover,by delivery with composite hydrogel,EV-NID1 is demonstrated to promote angiogenesis and bone regeneration in rat femoral defects.This study identifies the intracellular binding target of EV-NID1 and further elucidates a novel approach and mechanism,thereby providing a cell-free construction strategy with precise targets for the development of vascularized tissue engineering products.展开更多
Astrocytes can alter their appearance and become reactive following chronic cerebral ischemia. In the present study, a rat model of chronic cerebral ischemia was treated with 50 and 100 mg/kg curcumin. Results showed ...Astrocytes can alter their appearance and become reactive following chronic cerebral ischemia. In the present study, a rat model of chronic cerebral ischemia was treated with 50 and 100 mg/kg curcumin. Results showed that pathological changes of neuronal injury in hippocampal CA1 area of rats induced by chronic cerebral ischemia were attenuated, as well as upregulated expression of glial fibriliary acidic protein and nestin, in a dose-dependent manner.展开更多
基金We thank Suzanne Danley for editing the manuscript.This work was supported by a Research Grant from the National Institutes of Health,USA(No.1R01AR067747)to M.P.Health Commission of Sichuan Province(No.18PJ008)Science&Technology Department of Sichuan Province(No.2019YFS0267)to S.C.
文摘Basement membrane proteins are known to guide cell structures,differentiation,and tissue repair.Although there is a wealth of knowledge on the functions of laminins,per-lecan,and type IV collagen in maintaining tissue homeostasis,not much is known about nidogen.As a key molecule in the basement membrane,nidogen contributes to the formation of a delicate microenvironment that proves necessary for stem cell lineage-specific differentiation.In this review,the expression of nidogen is delineated at both cellular and tissue levels from embryonic to adult stages of development;the effect of nidogens is also summarized in the context of musculoskeletal development and regeneration,including but not limited to adipogenesis,angiogenesis,chondrogenesis,myogenesis,and neurogenesis.Furthermore,potential mechanisms underlying the role of nidogens in stem cell-based tissue regeneration are also discussed.This concise review is expected to facilitate our existing understanding and utilization of nidogen in tissue engineering and regeneration.
基金supported by the National Natural Science Foundation of China(Project no.81902202,81772377,81430049 and 81902263).
文摘The technique bottleneck of repairing large bone defects with tissue engineered bone is the vascularization of tissue engineered grafts.Although some studies have shown that extracellular vesicles(EVs)derived from bone marrow mesenchymal stem cells(BMSCs)promote bone healing and repair by accelerating angiogenesis,the effector molecules and the mechanism remain unclear,which fail to provide ideas for the future research and development of cell-free interventions.Here,we found that Nidogen1-enriched EV(EV-NID1)derived from BMSCs interferes with the formation and assembly of focal adhesions(FAs)by targeting myosin-10,thereby reducing the adhesion strength of rat arterial endothelial cells(RAECs)to the extracellular matrix(ECM),and enhancing the migration and angiogenesis potential of RAECs.Moreover,by delivery with composite hydrogel,EV-NID1 is demonstrated to promote angiogenesis and bone regeneration in rat femoral defects.This study identifies the intracellular binding target of EV-NID1 and further elucidates a novel approach and mechanism,thereby providing a cell-free construction strategy with precise targets for the development of vascularized tissue engineering products.
基金the National Natural Science Foundation of China, No. 30973154Chongqing Science and Technology Commission Foundation, No. 2009BB5270Chongqing Municipal Education Com-mission Foundation, No. KJ090301
文摘Astrocytes can alter their appearance and become reactive following chronic cerebral ischemia. In the present study, a rat model of chronic cerebral ischemia was treated with 50 and 100 mg/kg curcumin. Results showed that pathological changes of neuronal injury in hippocampal CA1 area of rats induced by chronic cerebral ischemia were attenuated, as well as upregulated expression of glial fibriliary acidic protein and nestin, in a dose-dependent manner.
