Traditional Nitrogen Removal Coupled with SND to Meet Advanced WWTP Standards at a Full Scale SBR Wastewater Treatment Facility

A Florida wastewater treatment facility studied how Simultaneous Nitrification Denitrification (SND) coupled with traditional nitrogen removal would be used to meet the state’s current advanced wastewater treatment nutrient criterion. This study examined the effect of these combined processes on the fate and transport of the nitrogen species during the treatment process. The effectiveness of nitrogen removal within the full scale sequential batch reactor system (SBR) and the extent of SND compared to nitrification and denitrification in the nitrogen removal process was also evaluated. Finally, the overall performance of the municipal wastewater treatment facility utilizing these combined processes was evaluated. Overall, this application reduced the total nitrogen to almost 6% of the permitted concentration of 3.0 mg/L. The combination of both processes also resulted in an actual ?concentration 93.7% lower than the acceptable theoretical ?concentration, which also resulted in effluent Total Inorganic Nitrogen nearly 80% lower than the permitted 3.0 mg/L effluent concentration. Further, the process produced a composite Total Nitrogen concentration that was 74% lower than the permitted concentration. This coupling of SND with traditional nitrogen removal resulted in a highly effective process to reduce nitrogen in the municipal wastewater effluent which is also attractive for potential implementation due to the low cost expenditure incurred in its utilization.

Nitrogen Derivatives of Irrigation in Chihuahua＇s Parks with Wastewater Treatment Residuals

Since 2000 Chihuahua City has distribution system Waste Water Treatment （WTR）, watering city＇s green areas. The need to cover the growing demand for drinking water has encouraged the use of non-potable water where water quality is not an issue despite being used by 10 years ago, it is not known whether nitrogen compounds derived from WTR pose risks to the environment and public health. Therefore, in order to minimize health and environmental risks, this is needed to assess the impact and policy support for water use. The aim of this study is to determine the constitution and the concentration of nitrogen compounds through selection and characterization of park＇s representative. It＇s divided into physical properties and main parameters that affect nitrogen transformations. It was determined that sand was material that allowed more favorable oxidation and reduction of nitrate in soil and atmosphere, opposite of the clay, When used WTR, nitrates, nitrites and ammonia nitrogen were identified in the subsurface and NOx and N20 were identified in the atmosphere. When drinking water was used, none of these compounds was found. However, it was determined that despite having high concentrations of nitrates, the WTR was within the limits allowed by the Mexican standard for the use of WTR in public places.

A modified active disturbance rejection control for a wastewater treatment process

Waste water treatment process(WWTP)control has been attracting more and more attention.However,various undesired factors,such as disturbance,uncertainties,and strong nonlinear couplings,propose big challenges to the control of a WWTP.In order to improve the control performance of the closed-loop system and guarantee the discharge requirements of the effluent quality,rather than take the model dependent control approaches,an active disturbance rejection control(ADRC)is utilized.Based on the control signal and system output,a phase optimized ADRC(POADRC)is designed to control the dissolved oxygen and nitrate concentration in a WWTP.The phase advantage of the phase optimized extended state observer(POESO),convergence of the POESO,and stability of the closed-loop system are analyzed from the theoretical point of view.Finally,a commonly accepted benchmark simulation model no.1.(BSM1)is utilized to test the POESO and POADRC.Linear active disturbance rejection control(LADRC)and the suggested proportion-integration(PI)control are taken to make a comparative research.Both system responses and performance index values confirm the advantage of the POADRC over the LADRC and the suggested PI control.Numerical results show that,as a result of the leading phase of the total disturbance estimation,the POESO based POADRC is an effective and promising way to control the dissolved oxygen and nitrate concentration so as to ensure the effluent quality of a WWTP.

Occurrence and Roles of Comammox Bacteria in Water and Wastewater Treatment Systems:A Critical Review

Nitrogen removal is a critical process in water treatment plants(WIPs)and wastewater treatment plants(WWTPs).The recent discovery of a novel bacterial process,complete ammonia oxidation(comammox,CMX),has refuted a century-long perception of the two-step conversion of NH3to NO3-.Compared with canonical nitrifiers,CMX bacteria offer undeniable advantages,such as a high growth yield propensity and adaptability to nutrient-and growth-limiting conditions,which collectively draw attention to validate the aptness of CMX bacteria to wastewater treatment.As there has been no comprehensive review on the relevance of CMX bacteria for sustainable water and wastewater treatment,this review is intended to discuss the roles and applications of CMX in the removal of nitrogen and pollutants from water and wastewater.We took into account insights into the metabolic versatilities of CMX bacteria at the clade and subclade levels.We focused on the distribution of CMX bacteria in engineered systems,niche differentiation,co-occurrence and interactions with cano nical nitrifiers for a better understanding of CMX bacteria in terms of their ecophysiology.Conceptualized details on the reactor adaptability and stress response of CMX bacteria are provided.The potential of CMX bacteria to degrade micropollutants either directly or co-metabolically was evaluated,and these insights would be an indispensable advantage in opening the doors for wider applications of CMX bacteria in WWTPs.Finally,we summarized future directions of research that are imperative in improving the understanding of CMX biology.

Research Progress on Energy Plants in Piggery Wastewater Treatment

Large-scale pig-raising can discharge a great deal of wastewater,which contains high content of organic matter,ammonia nitrogen and suspended solids.The improper treatment of the piggery wastewater can lead to serious environmental problems. As a liquid fertilizer,piggery wastewater is relatively low in fertilizer efficiency and high in transportation cost,so it is very necessary to treat it in situ. Energy plants have the advantages of rapid growth,large biomass,strong tillering ability and developed root system. Therefore,energy plants can be used to absorb and transform the pollutants( like nitrogen and phosphorus) in piggery wastewater into the components of plants,as well as form the rhizosphere environment which is conducive to microbial growth,so as to enhance the effects of nitrogen and phosphorus removal. The obtained energy plants can be recycled as the raw materials for biogas to increase the production of biogas,which brings economic benefits while solving the environmental problems caused by piggery wastewater.

Modification of Titanium Dioxide for Wastewater Treatment Application and Its Recovery for Reuse

Magnetic Fe3O4 nanomagnetic particles were synthesized by the titration co-precipitation method followed by coating by the sol-gel method with Titamiun dioxide. The photocalytic activities of different synthesized TiO2/Fe304 nanomagnetic particles with different molar ratios of TiO2 to Fe3O4 were investigated by the reduction of phosphate, nitrate and decolorizing of methyl blue solutions. X-ray diffraction was used to characterize the size, composition and morphology of the synthesized particles. The results obtained from these experiments indicate an increase in the photocatalytic activity as the amount of TiO2 coating increases. The results show a higher activity of the synthesized particles in the removal of phosphate, nitrate and methyl blue, which can be achieved at early reaction periods at about 70-80%. The activities were higher when the particles were incubated without UV illumination. This study shows that TiO2/Fe3O4 particles are effective in phosphate, nitrate and methyl blue removal in wastewater treatment.

Why Should We Bother on Measurement in Wastewater Treatment Operation?

The question of wastewater treatment and control is reflected from a very specific viewpoint: the low priority given to accurate and useful measurements within wastewater treatment. The matter is discussed from four various perspectives, that may be labelled “Legal understanding”;“Needs for accurate measurement results already in the planning and design stage”;“The measurement problem and human behaviour”;“The understanding of the short term and long term dynamics and changes in pollution and flow loads on a wastewater treatment plant (WWTP)”. All these aspects bring about much improved needs for an accurate and frequent measurement scheme both for pollutants and flows entering a WWTP. The conclusion is stated as follows: A far more and well elaborated on-line measurement system at the plants would become a needed tool for improved water environment protection at lower costs.

Experimental analysis of a nitrogen removal process simulation of wastewater land treatment under three different wheat planting densities

Nitrogen contaminant transport, transformation and uptake simulation experiments were conducted in green house under three different planting density of winter wheat. They were Group A, planting density of 0.0208 plants/cm 2, Group B, 0.1042 plants /cm 2, and Group C, 0.1415 plants/cm 2. The capacity and ratio of nitrogen removal were different on three kinds of conditions of wastewater land treatment. From analysis of wastewater treatment capacity, wastewater concentration and irrigation intensity for Group C were suitable and nitrogen quantity added was 2 times of that for Group B, 2.6 times for Group A while nitrogen residue was only 7.06%. Hence, wastewater irrigation and treatment design with purpose of waste water treatment should select the design with maximum capacity, optimal removal ratio and least residue in soil, which was closely related to crop planting density, crop growth status and also background nitrogen quantity in soil.

Optimize Operation of Partial Denitrification System for Simultaneous Treatment of Low C/N Municipal and Nitrate Wastewaters

In order to realize the simultaneous treatment of low C/N municipal and nitrate( NO3^--N) wastewaters,a sequencing batch reactor( SBR) was used to optimize the partial denitrification( PD),which the influent substrate and the anoxic reaction time were appropriately controlled. The carbon and nitrogen removal and the characteristic parameters of PD during long-term operation were studied. Experimental results showed that the PD showed stable characteristics of nitrogen and carbon removal and NO2^--N accumulation after an adaptation of 20 d with municipal wastewater used. The anoxic reaction time was extended from 50 to 70 min with the initial COD/NO3^--N decreased from 3. 0 to about 2. 5. When the influent NO3^--N was 117. 93 mg/L,the effluent NO2^--N and NAR were 23. 10 mg/L and 82. 26%,respectively,and the nitrogen and carbon removal rate reached 91. 76% and 65. 70%,respectively. The effluent NO2^--N/NH4^+ -N meantime reached 1.17-1. 22. Moreover,the cumulative concentration of NO2^--N and the system NAR increased linearly with the consumption of NO3^--N and COD,and the change trend was highly significant within 0-20 min,and gradually flattened.

Characterization of the genes involved in nitrogen cycling in wastewater treatment plants using DNA microarray and most probable number-PCR

To improve nitrogen removal performance of wastewater treatment plants （WWTPs）, it is essential to understand the behavior of nitrogen cycling communities, which comprise various microorganisms. This study characterized the quantity and diversity of nitrogen cycling genes in various processes of municipal WWTPs by employing two molecular-based methods：most probable number-polymerase chain reaction （MPN-PCR） and DNA microarray. MPN-PCR analysis revealed that gene quantities were not statistically different among processes, suggesting that conventional actwated sludge processes （CAS） are similar to nitrogen removal processes in their ability to retain an adequate population of nitrogen cycling microorganisms. Furthermore, most processes in the WWTPs that were researched shared a pattern：the nitS and the bacterial amoA genes were more abundant than the nirK and archaeal amoA genes, respectivelv. DNA microarray analysis revealed that several kinds of nitrification and denitrification genes were detected in both CAS and anaerobic-oxic processes （AO）, whereas limited genes were detected in nitrogen removal processes. Results of this study suggest that CAS maintains a diverse community of nitrogen cycling microorganisms; moreover, the microbial communities in nitrogen removal processes may be specific.

Treatment of Wastewater with Modified Sequencing Batch Biofilm Reactor Technology

This paper describes the removal of COD and nitrogen from wastewater with modified sequencing batch biofilm reactor. The strategy of simultaneous feeding and draining was explored.The results show that introduction of a new batch of wastewater and withdrawal of the purified water can be conducted simultaneously with the maximum volumetric exchange rate of about 70%.Application of this feeding and draining mode leads to the reduction of the cycle time, the increase of the utilization of the reactor volume and the simplification of the reactor structure. The treatment of a synthetic wastewater containing COD and nitrogen was investigated. The operation mode of F(D) O ( i.e ., simultaneous feeding and draining followed by the aerobic condition) was adopted. It was found that COD was degraded very fast in the initial reaction period of time, then reduced slowly and the ammonia nitrogen and nitrate nitrogen concentrations decreased and increased with time respectively, while the nitrite nitrogen level increased first and then reduced. The relationship between the COD or ammonia nitrogen loading and its removal rate was examined, and the removal of COD, ammonia nitrogen and total nitrogen could exceed 95%, 90% and 80% respectively. The fact that nitrogen could be removed more completely under constant aeration (aerobic condition) of the SBBR operation mode is very interesting and could be explained in several respects.

High nitrogen removal from wastewater with several new aerobic bacteria isolated from diverse ecosystems

Three new bacteria HS-03, HS-043 and HS-047 isolated from different ecosystems were found capable of aerobic denitrification. The potential application of these strains in wastewater treatment under aerobic conditions was investigated, These three bacteria all presented high nitrogen removal from wastewater that more than 98% of 10 mmol/L nitrate could be removed in 12--24 h by adding cheap external carbon source and low concentration of iron as well as molybdate. The mechanism at molecular level was analyzed. The success of this aerobic denitrification applied to wastewater treatment may serve as an alternative to enhance the practical nitrogen removal from wastewater. Main biochemical and physiological features of these strains were characterized. The 16S rDNA sequences were compared with the published data in GenBank by using BLAST. The results of phenotype and genotype proved that strain HS-03 and HS-047 belonged to Pseudomonas stutzeri and Pseudomonas pseudoalcaligenes respectively. Strain HS-043 was identified as Delftia clcidovorans of which denitrifying activity has not previously been explored.

Microbiome analysis and-omics studies of microbial denitrification processes in wastewater treatment: recent advances

Nitrogen pollution is an increasingly severe worldwide problem because of drainage of nitrogen-containing wastewater and intensive application of nitrogen-containing fertilizers. Denitrification, a key process in nitrogen cycles, is commonly employed for nitrogen removal in engineered wastewater treatment systems. Biological denitrification is performed by denitrifying microbes(bacteria) that use nitrate as terminal electron acceptor. Better understanding the functions of diverse microbial populations in denitrification-based wastewater treatment systems, and the interactions of these populations with operating environments, is essential for improving both treatment performance and system stability. Recent advances in "meta-omics"(e.g., genomics, transcriptomics, proteomics, metabolomics), other molecular biology tools, and microbiome analysis have greatly enhanced such understanding. This minireview summarizes recent findings regarding microbial community structure and composition, key functional microbes and their physiology, functional genes involved in nitrogen cycle, and responses of microbes and their genes to changes of environmental factors or operating parameters, in denitrification processes in wastewater treatment systems. Of particular interest are heterotrophic denitrification systems(which require alternative organic carbon sources) and the autotrophic denitrification systems(which do not require an external carbon source). Integrated microbiome and-omics approaches have great future potential for determination of optimal environmental and biotechnological parameters,novel process development, and improvement of nitrogen removal efficiency and system stability.

Research progress and prospects of complete ammonia oxidizing bacteria in wastewater treatment

Complete ammonia oxidizing bacteria,or comammox bacteria(CAOB),can oxidize ammonium to nitrate on its own.Its discovery revolutionized our understanding of biological nitrification,and its distribution in both natural and artificial systems has enabled a reevaluation of the relative contribution of microorganisms to the nitrogen cycle.Its wide distribution,adaptation to oligotrophic medium,and diverse metabolic pathways,means extensive research on CAOB and its application in water treatment can be promoted.Furthermore,the energy-saving characteristics of high oxygen affinity and low sludge production may also become frontier directions for wastewater treatment.This paper provides an overview of the discovery and environmental distribution of CAOB,as well as the physiological characteristics of the microorganisms,such as nutrient medium,environmental factors,enzymes,and metabolism,focusing on future research and the application of CAOB in wastewater treatment.Further research should be carried out on the physiological characteristics of CAOB,to analyze its ecological niche and impact factors,and explore its application potential in wastewater treatment nitrogen cycle improvement.

Inorganic nitrogen removal of toilet wastewater with an airlift external circulation membrane bioreactor

Removal of inorganic nitrogen （inorganic-N） from toilet wastewater, using a pilot-scale airlift external circulation membrane bioreactor （AEC-MBR） was studied. The results showed that the use of AEC-MBR with limited addition of alkaline reagents and volumetric loading rates of inorganic-N of 0.19-0.40 kg inorganic-N/（m^3·d） helped achieve the desired nitrification and denitrification. Furthermore, the effects of pH and dissolved oxygen （DO） on inorganic-N removal were examined. Under the condition of MLSS at 1.56-2.35 g/L, BODs/ammonia nitrogen （NH4＋-N） at 1.0, pH at 7.0-7.5, and DO at 1.0-2.0 mg/L, the removal efficiencies of NH4^＋-N and inorganic-N were 91.5% and 70.0%, respectively, in the AEC-MBR. The cost of addition of alkaline reagent was approximately 0.5-1.5 RMB yuan/m^3, and the energy consumption was approximately 0.72 kWh/m^3 at the flux of 8 L/（m^2-h）.

Removal of Nitrogen and Phosphorus from Saline Wastewater Using Up-Flow Sludge Blanket Filtration Process

There is recent trend of providing additional treatment of wastewater beyond tertiary level. The purpose is to refine water to a quality that is safe for reuse for unrestricted irrigation and other non potable uses. For this purpose, Kuwait has built and operated an advanced wastewater treatment plant with capacity of 500,000 m3·dl. This plant providing treatment beyond tertiary utilizes the process of Ultra Filtration （UF） and Reverse Osmosis （RO）. The reject water of this unit contains high concentration of total nitrogen and total phosphate. Safe disposal of this water into the environment or possible reuse needs substantial reduction of these chemicals. In this study, a bench scale up-flow sludge blanket filtration system was investigated. The system operated with an average Hydraulic-Retention Time （HRT） of 19 h, whereas, sludge age varied within the range of 14 days to 16.5 days. The results show that the average removal efficiencies of the system for Biological Oxygen Demand （BOD）, Chemical Oxygen Demand （COD） were over 86% and 82% respectively. The phosphate and nitrogen＇s average removal were found to be 50% and 45% respectively.

Fate of nitrogen in subsurface infiltration system for treating secondary effluent

The concentration of total nitrogen(TN) is reported to vary between 20 and 35 mg/L in domestic wastewater. In raw wastewater, ammonia nitrogen eNHt4-NT is the main nitrogen form, accounting for 70%e82% of the TN concentration. Organic nitrogen, nitrite nitrogen eNOà2-NT,and nitrate nitrogen eNOà3-NT are present as well. For years, due to the lack of regulatory limits on nitrogen concentration in surface waters,nitrogen from secondary effluent has posed a significant threat to the health of aquatic ecosystems. Researchers have made substantial efforts to reduce the nitrogen concentration in secondary effluent. As a kind of advanced wastewater treatment technology, the subsurface infiltration(SI)system has been widely used, owing to its advantages, which include low operation cost, easy maintenance, and low energy consumption. This review discusses the fate of various forms of nitrogen in SI treatment, including organic nitrogen, NHt4-N, NOà2-N, and NOà3-N. Major biological processes involved in nitrogen removal and the main factors influencing its transformation are suggested. Finally, it is shown that ammonification followed by nitrification-denitrification plays a major role in nitrogen removal. Further research needs to focus on the emission characteristics of gaseous nitrogen(generated from the nitrification, denitrification, and completely autotrophic nitrogen-removal over nitrite(CANON) processes) with respect to their greenhouse effects.

Research Development and Application of Nitrogen and Phosphorus Removal in Sequencing Batch Biofilm Reactor

Frequent variations of the wastewater quality and quantity and other uncertain factors are the challenges faced by many wastewater treatment plants during the operation. Sequencing batch biofilm reactor（ SBBR） process provides a new idea for an effective solution to this problem. This paper introduces the basic processes and characteristics of the sequencing batch biofilm reactor（ SBBR） process,and summarizes the research status of this process in wastewater treatment. Factors affecting the nitrogen and phosphorus removal effect of the SBBR process are also analyzed.

Feasibility of an innovative integrated process of simultaneous desulfurization and denitrification for high strength wastewater

An anaerobic expanding-bed reactor was adopted to investigate the feasibility of an innovative integrated process of simultaneous desulfurization and denitrification （SDD） for high strength wastewater. In the reactor, heterotrophic bacteria （including sulfate reducing bacterium and denitrifying bacteria） and autotrophic bacteria （ including Thiobacillus denitrificans） cooperated together by incubating and enriching functional bac- teria on different carriers in the anaerobic activated sludge. Synthetic wastewater with high concentrations of sulfate and nitrate was employed. The experimental resuhs showed that the removal efficiency of sulfate and nitrate was above 85% , elemental sulfur was observed while nitrate was absent in effluent. The balance of sulfur, nitrogen and electron was discussed respectively, which indicated that the integrated SDD process could be actualized. These resuhs might provide a guidance to further investigate the key factors affecting the integrated SDD process and to improve the efficiency of desulfurization and denitrification in wastewater treatment.

Characterization of the start-up period of single-step autotrophic nitrogen removal in a sequencing batch reactor

The characteristics of the start-up period of single-step autotrophic nitrogen removal process were investigated. The autotrophic nitrogen removal process used a sequencing batch reactor to treat wastewater of medium to low ammonia-nitrogen concentration, with dissolved oxygen （DO）, hydraulic retention time （HRT） and temperature controlled. The experimental conditions were temperature at （30±2） ℃, ammonia concentration of （60 to 120） mg/L, DO of （0.8 to 1.0） mg/L, pH from 7.8 to 8.5 and HRT of 24 h. The rates of nitrification and nitrogen removal turn out to be 77% and 40%, respectively, after a start up period going through three stages divided according to nitrite accumulation： sludge domestication, nitrifying bacteria selection and sludge adaptation, It is demonstrated that dissolved oxygen is critical to nitrite accumulation and elastic YJZH soft compound packing is superior to polyhedral hollow balls in helping the bacteria adhere to the membrane.