Nitrogen and phosphorus changes and optimal drainage time of flooded paddy field based on environmental factors

While many controlled irrigation and drainage techniques have been adopted in China, the environmental effects of these techniques require further investigation. This study was conducted to examine the changes of nitrogen and phosphorus of a flooded paddy water system after fertilizer application and at each growth stage so as to obtain the optimal drainage time at each growth stage. Four treatments with different water level management methods at each growth stage were conducted under the condition of ten-day continuous flooding. Results show that the ammonia nitrogen （ NH4-N ） concentration reached the peak value once the fertilizer was applied, and then decreased to a relatively low level seven to ten days later, and that the nitrate nitrogen （NO^-N） concentration gradually rose to its peak value, which appeared later in subsurface water than in surface water. Continuous flooding could effectively reduce the concentrations of NH^-N , NO3-N, and total phosphorus （TP） in surface water. However, the paddy water disturbance, in the process of soil surface adsorption and nitrification, caused NH]-N to be released and increased the concentrations of NH4-N and NO^-N in surface water. A multi-objective controlled drainage model based on environmental factors was established in order to obtain the optimal drainage time at each growth stage and better guide the drainage practices of farmers. The optimal times for surface drainage are the fourth, sixth, fifth, and sixth days after flooding at the tillering, jointing-booting, heading-flowering, and milking stages, respectively.

Nitrogen and Phosphorus Loss Law and Emission Reduction Effects Under Water and Fertilizer Management Integrated Mode in Dike Paddy Field

To achieve the purpose of reducing farm non-point source pollution, we integrated site specific nitrogen management precise irrigation, controlled drainage, and wetland eco-repair system in dike area of Taihu basin. During investigation, it had given prominence for the water and fertilizer coupling effects of precise irrigation and site specific nutrient management, the characteristics of integration on controlled irrigation, controlled drainage and wetland ecosystem non-point source pollution control. Then the water and fertilizer integrated management mode of paddy field was put forward in Taihu basin where the water production efficiency increased to 1.64 kg. m-3, water saved 37.8%, fertilizer use efficiency raised 15,4%, yield raised 10%, and N, P load decreased 26%-72%. The modern agricultural and farmland ecosystems that control and cut down the farm non-point source pollution came into being, which can be a reference by Taihu basin to control its agricultural non-point source pollution and eutrophicated water body.

Effects of nitrogen application rate and hill density on rice yield and nitrogen utilization in sodic saline–alkaline paddy fields

Soil salinity and alkalinity can inhibit crop growth and reduce yield,and this has become a global environmental concern.Combined changes in nitrogen (N) application and hill density can improve rice yields in sodic saline–alkaline paddy fields and protect the environment.We investigated the interactive effects of N application rate and hill density on rice yield and N accumulation,translocation and utilization in two field experiments during 2018 and 2019 in sodic saline–alkaline paddy fields.Five N application rates (0 (control),90,120,150,and 180 kg N ha^(-1) (N0–N4),respectively) and three hill densities(achieved by altering the distance between hills,in rows spaced 30 cm apart:16.5 cm (D1),13.3 cm (D2) and 10 cm (D3))were utilized in a split-plot design with three replicates.Nitrogen application rate and hill density significantly affected grain yield.The mathematical model of quadratic saturated D-optimal design showed that with an N application rate in the range of 0–180 kg N ha^(-1),the highest yield was obtained at 142.61 kg N ha^(-1) which matched with a planting density of 33.3×10^(4) ha^(-1).Higher grain yield was mainly attributed to the increase in panicles m^(–2).Nitrogen application rate and hill density significantly affected N accumulation in the aboveground parts of rice plants and showed a highly significant positive correlation with grain yield at maturity.From full heading to maturity,the average N loss rate of the aboveground parts of rice plants in N4 was 70.21% higher than that of N3.This is one of the reasons why the yield of N4 treatment is lower than that of the N3 treatment.Nitrogen accumulation rates in the aboveground parts under treatment N3 (150 kg N ha^(-1)) were 81.68 and 106.07% higher in 2018 and 2019,respectively,than those in the control.The N translocation and N translocation contribution rates increased with the increase in the N application rate and hill density,whereas N productivity of dry matter and grain first increased and then decreased with the increase in N application rate and hill density.Agronomic N-use efficiency decreased with an increase in N application rate,whereas hill density did not significantly affect it.Nitrogen productivity of dry matter and grain,and agronomic N-use efficiency,were negatively correlated with grain yield.Thus,rice yield in sodic saline–alkaline paddy fields can be improved by combined changes in the N application rate and hill density to promote aboveground N accumulation.Our study provides novel evidence regarding optimal N application rates and hill densities for sodic saline–alkaline rice paddies.

Nitrogen and Phosphorus Pollutants Removal from Rice Field Drainage with Ecological Agriculture Ditch: A Field Case

Excessive nitrogen and phosphorus in agricultural drainage can cause a series of water environmental problems such as eutrophication of water bodies and non-point source pollution.By monitoring the water purification effect of a paddy ditch wetland in Gaochun,Nanjing,Jiangsu Province,we investigated the spatial and temporal distribution patterns of N and P pollutants in paddy drains during the whole reproductive period of rice.Then,the dynamic changes of nitrogen and phosphorus in time and space during the two processes of rainfall after basal fertilization and topdressing were analyzed after comparison.At last,the effect of the ditch wetland on nutrient purification and treatment mechanism,along with changing flow and concentration in paddy drains,was clarified.The results of this study showed that the concentrations of various nitrogen and phosphorus in the ditch basically reached the peak on the second and third days after the rainfall(5.98 mg/L for TN and 0.21 mg/L for TP),which provided a response time for effective control of nitrogen and phosphorus loss.The drainage can be purified by the ecological ditch,about 89.61%,89.03%,89.61%,98.14%,and 79.05%of TN,NH4+-N,NO3−-N,NO2−-N,and TP decline.It is more effective than natural ditches for water purification with 80.59%,40%,12.07%,91.06%and 18.42%removal rates,respectively.The results of the study can provide a theoretical basis for controlling agricultural non-point source pollution and improving the water environment of rivers and lakes scientifically.

Study of Dynamics of Floodwater Nitrogen and Regulation of Its Runoff Loss in Paddy Field-Based Two-Cropping Rice with Urea and Controlled Release Nitrogen Fertilizer Application

The article deals with the effects of urea and controlled release nitrogen fertilizer （CRNF） on dynamics of pH, electronic conductivity （EC）, total nitrogen （TN）, NH4^＋-N and NO3 -N in floodwater, and the regulation of runoff TN loss from paddy field-based two-cropping rice in Dongting Lake, China, and probes the best fertilization management for controlling N loss. Studies were conducted through modeling alluvial sandy loamy paddy soil （ASP） and purple calcareous clayey paddy soil （PCP） using lysimeter, following the sequence of the soil profiles identified by investigating soil profile. After application of urea in paddy field-based two-cropping rice, TN and NHa＋-N concentrations in floodwater reached peak on the 1st and the 3rd day, respectively, and then decreased rapidly over time; all the floodwater NO3--N concentrations were very low; the pH of floodwater gradually rose in case of early rice within 15 d （late rice within 3 d） after application of urea, and EC remained consistent with the dynamics of NH4^＋-N. The applied CRNF, especially 70% CRNF, led to significantly lower floodwater TN and NH4^＋ concentrations, pH, and EC values compared with urea within 15 d after application. The monitoring result for N loss due to natural rainfall runoff indicated that the amount of TN lost in runoff from paddy field- based two-cropping rice with urea application in Dongting Lake area was 7.47 kg ha^-1, which accounted for 2.49% of urea- N applied, and that with CRNF and 70% CRNF application decreased 24.5 and 27.2% compared with urea application, respectively. The two runoff events, which occurred within 20 d after application, contributed significantly to TN loss from paddy field. TN loss due to the two runoffs in urea, CRNF, and 70% CRNF treatments accounted for 72, 70, and 58% of the total TN loss due to runoff over the whole rice growth season, respectively. And the TN loss in these two CRNF treatments due to the first run-off event at the 10th day after application to early rice decreased 44.9 and 44.2% compared with urea, respectively. In conclusion, the 15-d period after application of urea was the critical time during which N loss occurred due to high floodwater N concentrations. But CRNF decreased N concentrations greatly in floodwater and runoff water during this period. As a result, it obviously reduced TN loss in runoff over the whole rice growth season.

Adsorption and Desorption Characteristics of Nitrogen and Phosphorus in Paddy Soils from Different Parent Materials

Reddish clayey soil(HH),alluvial sandy soil(HS),granitic sandy soil(MS)and purple clayey soil(ZS)were used as the test materials to reveal the adsorption-desorption characteristics of nitrogen and phosphorus nutrients in paddy soils from different parent materials.The results showed that the nitrogen desorption amount of each soil was greater than the nitrogen adsorption amount in the low nitrogen concentration range of 0~10 mg/L;in the high nitrogen concentration range of 20~50 mg/L,the soil nitrogen desorption rate gradually decreased with the increase of nitrogen concentration of the equilibrium liquid;when the soil nitrogen adsorption amount was-57.267~352.400 mg/kg,the nitrogen adsorption capacity of the paddy soils from different parent materials was HS>ZS>HH>MS;when the nitrogen desorption amount was 8.367~37.833 mg/kg,the nitrogen desorption capacity of the paddy soils from different parent materials was HH>HS>MS>ZS;the nitrogen adsorption isothermal curves of HH,MS,HS and ZS fitted the Linear model,the correlation coefficients were 0.928~0.978.At the same time,in the range of low phosphorus concentration(0~10 mg/L),the phosphorus adsorption amounts of 4 paddy soils were greater than their phosphorus desorption amounts.When the phosphorus concentration of the equilibrium solution exceeded 10 mg/L,phosphorus fixation capacities of 4 paddy soils weakened,meanwhile their phosphorus desorption increased,but their adsorption amounts were still greater than their desorption amounts.When phosphorus adsorption and desorption amounts of 4 paddy soils were-110.312~534.961 and 0.188~14.320 mg/kg respectively,the phosphorus adsorption and desorption capacities of 4 paddy soils were HS>HH>ZS>MS and ZS>MS>HS>HH,respectively.The phosphorus adsorption isothermal curves of 4 paddy soils fitted the Langmuir and Freundlich models,the correlation coefficient were 0.945~0.995.In general,paddy soils developed from different parent materials in Hunan Province have different adsorption and desorption characteristics for nitrogen and phosphorus.Purple clayey soil has the strongest nitrogen fixation capacity due to its stronger viscosity,which can reduce the risk of nitrogen loss by effectively holding nitrogen in the soil solution.On the contrary,being of strong sandy property,granitic sandy soil has the worst nitrogen fixation capacity and higher risk of nitrogen loss.The four paddy soils have strong adsorption capacity and low desorption rate of phosphorus,which indicates that the main paddy soils in Hunan Province have strong adsorption capacity for phosphorus and relatively small loss risk.

Effects of Nitrogen Dosage on the Yield and Nitrogen Use Efficiency of Machine Transplanted Rice Using Dry Soil Preparation in Rice Paddy Field

[Objective] The aim was to analyze the effects of nitrogen dosage on the yield and nitrogen use efficiency of machine transplanted rice using the technology of dry soil preparation in rice paddy field. [Method] With conventional Japonica rice cultivar Shengdao 18 as the study material, the effect of nitrogen dosage on stem and tillers dynamics, yield components and nitrogen use efficiency were investigated using the technology of dry soil preparation in rice paddy field. [Result] The highest yield was 10 957.20 kg/hm^2 as the nitrogen application was 315.00 kg/hm^2. Meanwhile, the roughness ratio, grain-straw ratio and nitrogen use efficiency remained at a higher level. Low nitrogen application could not obtain high yield. In contrast, high nitrogen application quantity led to a significant decline in nitrogen use efficiency. [Conclusion] The study could provide a scientific basis for the further promotion of the technology of dry soil preparation in rice paddy field.

Bioturbation Effects of Benthic Fish on the Phosphorus Dynamic in Overlying Water of Paddy Field

[Objective]The research aimed to investigate the bioturbation effects of benthic fish Misgurnus anguillicaudatus on phosphorus dynamic in overlying water of paddy field,as well as to explore the bioturbation mechanism.[Method]Based on simulation experiment,the phosphorus contents in overlying water were analyzed comparatively with and without Misgurnus anguillicaudatus by the using of ion chromatography and spectrophotometry.[Result]The concentrations of total phosphorus(TP),dissolved total phosphorus(DTP)and particular phosphorus(PP)in bioturbation group had no significant differences with those in control group in initial stage of experiment,and became significantly higher than control group in middle and late stages of experiment(P<0.05). The PP/TP ratios in bioturbation group were bigger than those in control group,the increase of TP concentration in bioturbation group was mainly due to the increase of PP. The ratios of dissolved inorganic phosphorus(DIP) to DTP (DIP/DTP) were significantly bigger than those in control group in middle and late stages of experiment(P<0.05).[Conclusion]The benthic fish had bioturbation effects on phosphorus in overlying water of paddy field,which increased the available phosphorus for rice growth.

Effects of straw and biochar addition on soil nitrogen,carbon,and super rice yield in cold waterlogged paddy soils of North China

The additions of straw and biochar have been suggested to increase soil fertility, carbon sequestration, and crop produc- tivity of agricultural lands. To our knowledge, there is little information on the effects of straw and biochar addition on soil nitrogen form, carbon storage, and super rice yield in cold waterlogged paddy soils. We performed field trials with four treatments including conventional fertilization system （CK）, straw amendment 6 t ha^-1 （S）, biochar amendment 2 t ha^-1 （C1）, and biochar amendment 40 t ha^-1 （C2）. The super japonica rice variety, Shennong 265, was selected as the test Crop. The results showed that the straw and biochar amendments improved total nitrogen and organic carbon content of the soil, reduced N2O emissions, and had little influence on nitrogen retention, nitrogen density, and CO2 emissions. The S and C1 increased NH4^＋-N content, and C2 increased NO3^--N content. Both S and C1 had little influence on soil organic carbon density （SOCD） and C/N ratio. However, C2 greatly increased SOCD and C/N ratio. C1 and C2 significantly improved the soil carbon sequestration （SCS） by 62.9 and 214.0% （P〈0.05）, respectively, while S had no influence on SCS. C1 and C2 maintained the stability of super rice yield, and significantly reduced CH4 emissions, global warming potential （GWP）, and greenhouse gas intensity （GHGI）, whereas S had the opposite and negative effects. In summary, the biochar amendments in cold waterlogged paddy soils of North China increased soil nitrogen and carbon content, improved soil carbon sequestration, and reduced GHG emission without affecting the yield of super rice.

Non-Point-Source Nitrogen and Phosphorus Loadings from a Small Watershed in the Three Gorges Reservoir Area

Non-point-source pollution has become a major threat to the water quality of the Three Gorges Reservoir(TGR);however,nutrient loadings from terrestrial sources are unclear due to a lack of in situ monitoring.A representative small watershed in the central part of the TGR area was selected to monitor the loss of nitrogen(N) and phosphorous(P) continuously along with the runoff from 2007 through 2009 to understand the exact sources and loadings.Results show that the non-point-source nitrogen and phosphorus comes mainly from the storm runoff from residential areas,citrus orchards and sloping croplands,which contributes up to 76% of the loadings in this watershed.Thus,a crucial measure for controlling non-point-source pollution is to intercept storm runoff from the three sources.Paddy fields provide a sink for non-point-source N and P by intercepting the runoff and sediment along with their different forms of nitrogen and phosphorus.The N and P removal efficiency by paddy fields from residential areas is within the range of 56% to 98%.Paddy fields are an important land-use option for reducing the non-point-source loading of N and P in the TGR area.

Nitrogen interception in floodwater of rice field in Taihu region of China

A field experiment located in Taihu Lake Basin of China was conducted, by application of urea or a mixture of urea with manure, to elucidate the interception of nitrogen （N） export in a typical rice field through ＂zero-drainage water management＂ combined with sound irrigation, rainfall forecasting and field drying. N concentrations in floodwater rapidly declined before the first event of field drying after three split fertilizations, and subsequently tended to rearm to the background level. Before the first field drying, total particulate nitrogen （TPN） was the predominant N form in floodwater of plots with no N input, dissolved inorganic nitrogen （DIN） on plots that received urea only, and dissolved organic nitrogen （DON） on plots treated with the mixture of urea and manure. Thereafter TPN became the major form. No N export was found from the rice field, but total nitrogen （TN） of 15.8 kg/hm^2 was remained, mainly due to soil N sorption. The results recommended the zero-drainage water management for full-scale areas for minimizing N export.

Measurement of Ammonia Emission Following Surface Application of Urea Fertilizer from Irrigated Paddy Rice Fields

Ammonia emission is one of the most important pathways of nitrogen loss from agricultural cultivated field. In this paper, we report the measurement of ammonia emission from paddy rice field obtained by surface application of urea fertilizer with water management. The main objective of the present study were to assess the amount of NH3 emission and the loss of nitrogen from paddy field as affected by various N doses, i.e., 0 (control), 90 (N1), 180 (N2), 270 (N3) and 360 (N4) kg ha-1, following field surface application of urea fertilizer with water management. Ammonia emissions were measured by continuous airflow enclosure method from plots fertilized with the application of surface urea. Increase in urea-N dosage increased NH3 emission that was measured from paddy rice field. Ammonia emission started immediately and was almost complete within 12 days after top dressing of urea application to the soils. Ammonia emissions were nearly constant in all treatments from 12 days after fertilizer application. Highest ammonia emission rate was 28 g /day and total amount of ammonia emission was 56.21 kg ha-1 for 360 kg N ha-1 dose. No remarkable observation was found about temperature for ammonia emission. Due to proper water management practices less emission was observed throughout the experiment period. The results also show that N loss through NH3 emission accounted for 11 to 16% during the rice- growing season. These magnitudes of loss of N appear to be most important for environmental point of view.

Dry deposition of ammonia around paddy fields in the subtropical hilly area in southern China

This study measured the ammonia(NH3)concentration and dry deposition within 100 m around paddy fields(0.6 ha)with double rice cropping in the subtropical hilly area in southern China,with the aims to quantify the dry deposition of NH3 around the emission source and to clarify its temporal and spatial variability.The results showed that high NH3 concentrations were found during the 15 d after nitrogen(N)fertilizer application at downwind sites within 100maround the paddy fields,and the NH3 concentrations were 12–62,2.8–7.3,13–38,and 4.9–36μg N m−3 during the 15 d after basal fertilizer application and topdressing in the early rice season and after basal fertilizer application and topdressing in the late rice season,respectively.The NH3 concentrations were relatively low(1.5–-3.8μg N m−3)during other periods of the rice season at the downwind sites,which indicated that N fertilizer application in paddy fields highly affected the NH3 concentration at downwind sites.The NH3 concentrations at the downwind sites decreased significantly with the increase in distance from the paddy fields.The total NH3 dry deposition around 100 m of the paddy fields accounted for approximately 79%and 81%of the emitted NH3 from the paddy fields in the early and late rice seasons,respectively.The results indicate that dry deposition of NH3 around emission sources may be an important way to remove the NH3 volatilized from croplands in this subtropical hilly area.

Effects of Green Manure Rotation on Rice Growth Dynamics and Nitrogen Uptake and Utilization

This study aimed to comprehensive evaluation of different winter green manure on characterization of nitrogen uptake and utilization, to provide the basis for N fertilizer reasonable operation and characteristics improvement of nitrogen nutrition in rice high-yield cultivation. This experiment was set to compare milk vetch, rapeseed,ryegrass and mixed of green manure on rice yield, rice growth dynamics and nitrogen uptake and utilization in rice. The results showed that among 4 different winter green manure, the treatment of MV-R-R（milk vetch-rice-rice） for the early rice yield was the most than others treatments. Compared with RG-R-R（ryegrass-rice-rice）, RPR-R（rapeseed-rice-rice）, MS-R-R（mixed green manure-rice-rice）, the treatment of MVR-R inceased by 6.61%, 3.29%, 0.78%, respectively. The treatment of MV-R-R in N content in plant of rice was maximized in the tillering, booting, heading and maturity periods, respectively higher than the average of other treatments 9.68%, 19.72%,6.23% and 8.66%. At tillering, booting, heading and maturity, the treatment of MV-R-R were the highest in N uptake, RP-R-R minimum. The N periodic accumulation for MV-R-R were higher than other treatments in the tillering to booting, booting to heading and heading to maturity periods. The rates respectively were 21.81%, 68.73% and286.5%. In addition, N periodic accumulation and its ratio to total in the heading to maturity was minimum, maximum before tillering under green manure rotation system.So the cropping system of milk vetch-rice-rice could increase nitrogen use efficiency and improve N cycling.

Field experiments on greenhouse gas emissions and nitrogen and phosphorus losses from rice paddy with efficient irrigation and drainage management

Greenhouse gas emissions,nitrogen and phosphorous losses through ammonia volatilization,leaching and surface drainage from rice paddy under efficient irrigation and drainage were analyzed based on field experimental data in order to reveal the eco-environmental impacts of efficient irrigation and drainage on rice paddy.The results showed that total methane emission from rice paddy under the controlled irrigation was reduced by more than 80% and total nitrous oxide emission increased by 15.9% compared with flooding irrigation.Seasonal comprehensive global warming potentials(GWP) of methane and nitrous oxide were 62.23 gCO2 m-2 for rice paddy under the controlled irrigation,reduced by 68.0% compared with flooding irrigation.Due to large reduction in seepage and surface drainages,nitrogen and phosphorous losses through leaching were reduced by 40.1% and 54.8%,nitrogen and phosphorous losses through surface drainage were reduced by 53.9% and 51.6% from rice paddy under efficient irrigation and drainage compared with traditional irrigation and drainage.Nitrogen loss through ammonia volatilization was reduced by 14.0%.Efficient irrigation and drainage management is helpful to mitigate greenhouse gases emission,nitrogen and phosphorus losses and their pollution on groundwater and surface water.

Nitrogen-based fertilizers differentially affect protist community composition in paddy field soils

Protists are one of the most diverse and dominant microbial groups and they play critical roles in the soil ecosystem.Although nitrogen fertilizers have a profound impact on protist communities,still less is known about how different nitrogen fertilizer types affect protist community composition in different soil types.Here we investigated the effects of six inorganic nitrogen fertilizers(urea,ammonium nitrate,ammonium sulfate,potassium nitrate,ammonium chloride,and diammonium hydrogen phosphate)and an organic fertilizer(a mixture of rice husk and cow manure)on protist community composition in three paddy field soils using a high-throughput sequencing method.The effect of the fertilizers on the functional groups of protists,namely consumers(predators and decomposers),photoautotrophs,and parasites(plant pathogens and animal parasites)was also analyzed.The results showed that nitrogen fertilizers had distinctive effects on the beta diversity of the protists,while we also observed that the same fertilizer had slightly different effects depending on the soil type.Amoebozoa and Rhizaria were the most affected protist taxonomical groups,while predatory protists were the main functional groups that were affected by nitrogen fertilizers.Random forest analysis showed that most of the fertilizer-affected protists were predators,among which Cercozoa was the most affected taxa.In conclusion,our results provide important insights into the impact of nitrogen fertilizers on soil protist communities.

Integrated rice management simultaneously improves rice yield and nitrogen use efficiency in various paddy fields

The hilly area of Southwest China is a typical rice production area which is limited by seasonal droughts and low temperature in the early rice growth period.A field experiment was conducted on three typical paddy fields(low-lying paddy field,medium-elevation paddy field,and upland paddy field)in this region.Nitrogen(N)treatment(180 kg N ha-1 year-1)was compared to a control treatment(0 kg N ha-1 year-1)to evaluate the effects of integrated rice management(IRM)on rice growth,grain yield,and N utilization.Integrated rice management integrated raised beds containing plastic mulch,furrow irrigation,and triangular transplanting.In comparison to traditional rice management(TRM),IRM promoted rice tiller development,with 7–13 more tillers per cluster at the maximum tillering stage and 1–6 more tillers per cluster at the end of tillering stage.Integrated rice management significantly increased the rice aboveground biomass by 34.4%–109.0%in different growth periods and the aboveground N uptake by 25.3%–159.0%.Number of productive tillers significantly increased by 33.0%,resulting in a 33.0%increase in grain yield and 8.0%improvement of N use efficiency(NUE).Grain yields were significantly increased in all three paddy fields assessed,with IRM being the most important factor for grain yield and productive tiller development.Effects of paddy field type and N level on N uptake by aboveground plants were reflected in the rice reproductive growth period,with the effects of IRM more striking due to the dry climate conditions.In conclusion,IRM simultaneously improved rice yield and NUE,presenting a valuable rice management technique in the paddy fields assessed.

How silicon fertilizer improves nitrogen and phosphorus nutrient availability in paddy soil?

In order to reveal the mechanism of silicon(Si)fertilizer in improving nitrogen(N)and phosphorus(P)nutrient availability in paddy soil,we designed a series of soil culture experiments by combining application of varying Si fertilizer concentrations with fixed N and P fertilizer concentrations.Following the recommendations of fertilizer manufacturers and local farmers,we applied Si in concentrations of 0,5.2,10.4,15.6,and 20.8μg/kg.At each concentration of added Si,the availability of soil N and P nutrients,soil microbial activity,numbers of ammonia-oxidizing bacteria and P-decomposing bacteria which means that the organic P is decomposed into inorganic nutrients which can be absorbed and utilized by plants,and urease and phosphatase activity first increased,and then decreased,as Si was added to the soil.These indicators reached their highest levels with a Si application rate of 15.6μg/kg,showing values respectively 19.78%,105.09%,8.34%,73.12%,130.36%,28.12%,and 20.15%higher than those of the controls.Appropriate Si application(10.4 to 15.6µg/kg)could significantly increase the richness of the soil microbial community involved in cycling of N and P nutrients in the soil.When the Si application rate was 15.6μg/kg,parameters for characterizing microbial abundance such as sequence numbers,operational taxonomic unit(OTU)number,and correlation indices of microbial community richness such as Chao1 index,the adaptive coherence estimator(ACE)index,Shannon index,and Simpson index all reached maximum values,with amounts increased by 14.46%,10.01%,23.80%,30.54%,0.18%,and 2.64%,respectively,compared with the control group.There is also a good correlation between N and P mineralization and addition of Si fertilizer.The correlation coefficients between the ratio of available P/total P(AP/TP)and the number of ammonia-oxidizing bacteria,AP/TP and acid phosphatase activity(AcPA),AP/TP and the Shannon index,the ratio of available N/total amount of N(AN/TN)and the number of ammoniated bacteria,and AN/TN and AcPA were 0.9290,0.9508,0.9202,0.9140,and 0.9366,respectively.In summary,these results revealed that enhancement of soil microbial community structure diversity and soil microbial activity by appropriate application of Si is the key ecological mechanism by which application of Si fertilizer improves N and P nutrient availability.

应用DRAINMOD-NⅡ模型对暴雨后稻田排水量和氮素模拟

为研究暴雨后稻田氮素的排放过程,在宿迁市船行灌区开展了试验,测定了常规灌排和控制灌排模式下暴雨后NH_4^+—N、NO_3^-—N的浓度和稻田排水量,并用DRAINMOD-N Ⅱ模型对暴雨后稻田排水量、NH_4^+—N和NO_3^-—N流失负荷进行模拟。试验结果表明:控制灌排模式可以显著减少雨后稻田排水量和氮素排放量;在两种灌排模式下,DRAINMOD-N Ⅱ模型对雨后排水量模拟值与测量值的相对误差RE在2%以内,相关系数R在0.98以上,模拟效率系数NS在0.96以上,对NH_4^+—N和NO_3^-—N流失负荷的模拟值与测量值的相对误差在5%以内,相关系数R在0.95以上,模拟效率系数NS在0.90以上,因此通过模型率定得到的各参数值较为合理,可以利用该DRAINMOD-NⅡ模型对未来暴雨后稻田排水量、NH_4^+—N和NO_3^-—N流失负荷进行预测。

Can arbuscular mycorrhiza and fertilizer management reduce phosphorus runoff from paddy fields?

Our study sought to assess how much phosphorus（P） runoff from paddy fields could be cut down by fertilizer management and inoculation with arbuscular mycorrhizal fungi. A field experiment was conducted in Lalin River basin, in the northeast China： six nitrogen-phosphorus-potassium fertilizer levels were provided（0, 20%, 40%, 60%, 80%, and 100% of the recommended fertilizer supply）, with or without inoculation with Glomus mosseae. The volume and concentrations of particle P（PP） and dissolved P（DP） were measured for each runoff during the rice growing season. It was found that the seasonal P runoff, including DP and PP, under the local fertilization was 3.7 kg/ha, with PP, rather than DP, being the main form of P in runoff water. Additionally, the seasonal P runoff dropped only by 8.9% when fertilization decreased by 20%; rice yields decreased with declining fertilization. We also found that inoculation increased rice yields and decreased P runoff at each fertilizer level and these effects were lower under higher fertilization. Conclusively, while rice yields were guaranteed arbuscular mycorrhizal inoculation and fertilizer management would play a key role in reducing P runoff from paddy fields.