The rice cultivars carrying dep1(dense and erect panicle 1)have the potential to achieve both high grain yield and high nitrogen use efficiency(NUE).However,few studies have focused on the agronomic and physiological ...The rice cultivars carrying dep1(dense and erect panicle 1)have the potential to achieve both high grain yield and high nitrogen use efficiency(NUE).However,few studies have focused on the agronomic and physiological performance of those cultivars associated with high yield and high NUE under field conditions.Therefore,we evaluated the yield performance and NUE of two near-isogenic lines(NILs)carrying DEP1(NIL-DEP1)and dep1-1(NIL-dep1)genes under the Nanjing 6 background at 0 and 120 kg N ha^(–1).Grain yield and NUE for grain production(NUEg)were 25.5 and 21.9%higher in NIL-dep1 compared to NIL-DEP1 averaged across N treatments and planting years,respectively.The yield advantage of NIL-dep1 over NIL-DEP1 was mainly due to larger sink size(i.e.,higher total spikelet number),grainfilling percentage,total dry matter production,and harvest index.N utilization rather than N uptake contributed to the high yield of NIL-dep1.Significantly higher NUEg in NIL-dep1 was associated with higher N and dry matter translocation efficiency,lower leaf and stem N concentration at maturity,and higher glutamine synthetase(GS)activity in leaves.In conclusion,dep1 improved grain yield and NUE by increasing N and dry matter transport due to higher leaf GS activity under field conditions during the grain-filling period.展开更多
NADP（H）-dependent glutamate dehydrogenases（GDH） in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri...NADP（H）-dependent glutamate dehydrogenases（GDH） in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions.These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice.展开更多
基金supported by the National Natural Science Foundation of China(32071948)the Fundamental Research Funds for the Central Universities,China(2662020ZKPY015)the Guangxi Natural Science Foundation,China(2022GXNSFAA035488)。
文摘The rice cultivars carrying dep1(dense and erect panicle 1)have the potential to achieve both high grain yield and high nitrogen use efficiency(NUE).However,few studies have focused on the agronomic and physiological performance of those cultivars associated with high yield and high NUE under field conditions.Therefore,we evaluated the yield performance and NUE of two near-isogenic lines(NILs)carrying DEP1(NIL-DEP1)and dep1-1(NIL-dep1)genes under the Nanjing 6 background at 0 and 120 kg N ha^(–1).Grain yield and NUE for grain production(NUEg)were 25.5 and 21.9%higher in NIL-dep1 compared to NIL-DEP1 averaged across N treatments and planting years,respectively.The yield advantage of NIL-dep1 over NIL-DEP1 was mainly due to larger sink size(i.e.,higher total spikelet number),grainfilling percentage,total dry matter production,and harvest index.N utilization rather than N uptake contributed to the high yield of NIL-dep1.Significantly higher NUEg in NIL-dep1 was associated with higher N and dry matter translocation efficiency,lower leaf and stem N concentration at maturity,and higher glutamine synthetase(GS)activity in leaves.In conclusion,dep1 improved grain yield and NUE by increasing N and dry matter transport due to higher leaf GS activity under field conditions during the grain-filling period.
基金supported by the National Natural Science Foundation of China (31571635)the Important National Science and Technology Specific Projects (2009ZX08001-030B)+2 种基金the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486)the Planned Science and Technology Project of Hunan Province (2014WK2005)the Public Subject of State Key Laboratory of Rice Biology (150103)
文摘NADP（H）-dependent glutamate dehydrogenases（GDH） in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions.These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice.
