[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds w...[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.展开更多
β-C<sub>3</sub>N<sub>4</sub> compound is a new hard material predicted by Liu and Cohen with theoretical calculation and so far it has not been found out in nature. In was assumed that the β-...β-C<sub>3</sub>N<sub>4</sub> compound is a new hard material predicted by Liu and Cohen with theoretical calculation and so far it has not been found out in nature. In was assumed that the β-C<sub>3</sub>N<sub>4</sub> compound would adopt the known crystal structure of β-Si<sub>3</sub>N<sub>4</sub>, which constructed a network of CN<sub>4</sub> tetrahedra linked at the corners by threefold coordinate N atoms. Thus, the atomic coordination of β-C<sub>3</sub>N<sub>4</sub> is sp<sup>3</sup> hybrids on the C atoms and sp<sup>2</sup> hybrids on the N atoms. Based on the theoretical analysis and modeling calculation, it展开更多
基金Supported by National Natural Science Foundation of China(308200204)Natural Science Research Program in Education Department of Henan Province(2009B180019)~~
文摘[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.
基金Project supported by the National Natural Science Foundation of China and 853' Committee.
文摘β-C<sub>3</sub>N<sub>4</sub> compound is a new hard material predicted by Liu and Cohen with theoretical calculation and so far it has not been found out in nature. In was assumed that the β-C<sub>3</sub>N<sub>4</sub> compound would adopt the known crystal structure of β-Si<sub>3</sub>N<sub>4</sub>, which constructed a network of CN<sub>4</sub> tetrahedra linked at the corners by threefold coordinate N atoms. Thus, the atomic coordination of β-C<sub>3</sub>N<sub>4</sub> is sp<sup>3</sup> hybrids on the C atoms and sp<sup>2</sup> hybrids on the N atoms. Based on the theoretical analysis and modeling calculation, it
