A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-et...A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-ethylcarbodiimide hydrochloride（EDC）and N-hydroxysuccinimide（NHS）.By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25（n=8,γ=0.998 5）and the detection limit as 3.60×10-8 mol/L（3σ）.展开更多
Purpose:First,the article offers a critical examination of the Deweyan conception of“common faith”in the context of climate change.Second,the article explores the conceptual linkages among the Confucian conception o...Purpose:First,the article offers a critical examination of the Deweyan conception of“common faith”in the context of climate change.Second,the article explores the conceptual linkages among the Confucian conception of the human–nature unity,the Buddhist doctrine of“no-self,”and the Deweyan conception of common faith.Third,the article proposes a transformative pedagogical praxis that welcomes and embraces the pursuit of the intra-and intergenerational justice in this Anthropocene Age of climate change.Design/Approach/Methods:This study is based on a philosophical inquiry into interrelated issues concerning the cultivation of common faith in the age of climate change.Findings:The Confucian conception of a human–nature unity,the Buddhist doctrine of“noself,”and the Deweyan“common faith,”collectively in recognition of a coterminous coexistence of humans and the universe,can shed light on the development of a transformative climate pedagogy.Further,embracing a dialogical pluriversality,recognizing human fallibility,can cultivate a shared agency and ecological identity.Originality/Value:Grounded in the coterminous coexistence of humans and the universe,the conceptual linkages among the Confucian conception of the unity of humans and nature,the Buddhist doctrine of“no-self,”and the Deweyan common faith reveal the possibility of crosscultural collaboration for our interdependent future.展开更多
文摘A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-ethylcarbodiimide hydrochloride（EDC）and N-hydroxysuccinimide（NHS）.By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25（n=8,γ=0.998 5）and the detection limit as 3.60×10-8 mol/L（3σ）.
文摘Purpose:First,the article offers a critical examination of the Deweyan conception of“common faith”in the context of climate change.Second,the article explores the conceptual linkages among the Confucian conception of the human–nature unity,the Buddhist doctrine of“no-self,”and the Deweyan conception of common faith.Third,the article proposes a transformative pedagogical praxis that welcomes and embraces the pursuit of the intra-and intergenerational justice in this Anthropocene Age of climate change.Design/Approach/Methods:This study is based on a philosophical inquiry into interrelated issues concerning the cultivation of common faith in the age of climate change.Findings:The Confucian conception of a human–nature unity,the Buddhist doctrine of“noself,”and the Deweyan“common faith,”collectively in recognition of a coterminous coexistence of humans and the universe,can shed light on the development of a transformative climate pedagogy.Further,embracing a dialogical pluriversality,recognizing human fallibility,can cultivate a shared agency and ecological identity.Originality/Value:Grounded in the coterminous coexistence of humans and the universe,the conceptual linkages among the Confucian conception of the unity of humans and nature,the Buddhist doctrine of“no-self,”and the Deweyan common faith reveal the possibility of crosscultural collaboration for our interdependent future.
