Quantitative Trait Loci Underlying Seed Sugars Content in “MD96-5722” by “Spencer” Recombinant Inbred Line Population of Soybean

Sucrose, raffinose, and stachyose are important soluble sugars in soybean [Glycine max (L.) Merr.] seeds. Seed sucrose is a desirable trait for taste and flavor. Raffinose and stachyose are undesirable in diets of monogastric animals, acting as anti-nutritional factors that cause flatulence and abdominal discomfort. Therefore, reducing raffinose and stachyose biosynthesis is considered as a key quality trait goal in soy food and feed industries. The objective of this study was to identify genomic regions containing quantitative trait loci (QTL) controlling sucrose, raffinose, and stachyose in a set of 92 F5:7 recombinant inbred lines (RILs) derived from a cross between the lines “MD96-5722” and “Spencer” by using 5376 Single Nucleotide Polymorphism (SNP) markers from the Illumina Infinium SoySNP6K BeadChip array. Fourteen significant QTL were identified and mapped on eight different linkage groups (LGs) and chromosomes (Chr). Three QTL for seed sucrose content were identified on LGs N (Chr3), K (Chr9), and E (Chr15). Seven QTL were identified for raffinose content on LGs D1a (Chr1), N (Chr3), C2 (Chr6), K (Chr9), B2 (Chr14), and J (Chr16). Four QTL for stachyose content were identified on LG D1a (Chr1), C2 (Chr6), H (Chr12), and B2 (Chr14). Selection for beneficial alleles of these QTLs could facilitate breeding strategies to develop soybean lines with higher concentrations of sucrose and lower levels of raffinose and stachyose.

肥胖儿童non-HDL-C、remnant cholesterol与亚临床动脉粥样硬化的关系

目的:了解non-HDL-C和remnant cholesterol与肥胖儿童亚临床动脉粥样硬化(AS)的关系。方法:根据甘油三酯(TG)水平将65例肥胖儿童(肥胖组)分为I组(TG≥2.3 mmol/L)32例,II组(TG<2.3 mmol/L)33例,与肥胖组儿童性别年龄相匹配的正常体质量儿童30例为III组(对照组)。比较3组儿童血脂指标,对肥胖组各项血脂指标与血浆致动脉粥样硬化指数(AIP)进行相关性分析,肥胖患儿体质指数、血脂指标与颈动脉内径、内膜-中层厚度(c-IMT)、血流参数进行相关性分析。结果:I组、II组的TG、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、non-HDL-C、remnant cholesterol、AIP均较对照组升高,高密度脂蛋白胆固醇(HDL-C)较对照组降低,且I组的remnant cholesterol、AIP均较II组升高。肥胖组儿童AIP与TG、non-HDL-C、remnant cholesterol呈正相关,与HDL-C呈负相关。37例行颈动脉超声检查的肥胖儿童的c-IMT较正常儿童的增厚。结论:non-HDL-C、remnant cholesterol在肥胖儿童亚临床AS中有重要作用,在肥胖儿童血脂异常的治疗中应关注non-HDL-C、remnant cholesterol的变化。

Shenqi Fuzheng injection combined with GP chemotherapy in the treatment of advanced non-small cell lung cancer： a meta-analysis

Objective： To evaluate the clinical efficacy of Shenqi Fuzheng injection combined with gemcitabine plus cisplatin（GP） in the treatment of advanced non-small cell lung cancer （NSCLC）. Methods： we performed a systematicsearch in the electronic databases such as Cochrane Library, Pubmed, Embase, Chinese Journal Full-text Database,Chinese Biomedical Literature Database, Chinese Science and Technology Periodical Full-text Database andWanfang Database up to 30 January 2017. Randomized controlled trials （RCT） of Shenqi Fuzheng Injectioncombined with GP chemotherapy in the treatment of advanced NSCLC were searched, and all the RCTs wereconducted on methodological quality assessment. Data extraction and data analysis were according to standards ofCochrane systematic review. Results： Eight trials were included including a total of 701 patients. Meta-analysisresults： Shenqi Fuzheng injection combined with GP chemotherapy could significantly improve the functionalstatus of patients with NSCLC （OR = 3.44, 95% CI [2.26, 5.25], P 〈 0.0001） and clinical treatment efficacy （OR =（OR = 0.31, 95%CI [0.20, 0.47], P 〈 0.0001. The rate of leukopenia （OR = .31, 95%CI [0.20,0.47], P 〈 0.0001）,thrombocytopenia （OR = 0.58, 95%CI [0.37, 0.91], P = 0.020）, hemoglobin decline （（OR = 0.31, 95%CI [0.16,0.59], P = 0.0004） and incidence of gastrointestinal reactions （OR = 0.58,P 〈 0.05） could be reduced. Conclusion：Shenqi Fuzheng injection combined with GP chemotherapy in the treatment of advanced NSCLC obtainedsignificantly clinical efficacy. The quality of the literature incorporated is low, the conclusion requires high-qualityresearch to further prove.

Analysis of Numerical Results in High Temperature Congealment and Chemistry Non-equilibrium Flow Field

Using the air plasma ignition technique, physicochemical process of burning can be accelerated; concentration limit ofretrofires both can be extended; reliability of retrofires and stability of burning can be improved. In this paper, using internalequivalent heat area in place of electric are that created Ohm heat, the flow fields of thermodynamic equilibrium chemistry con-gealment and chemistry non-equilibrium in the plasma generator were simulated. The influences of the inlet prerotation angleof air, the inlet total pressure of air and the airflow compression angle of spray nozzle on the temperature on the surface of elec-

Turbulent Flow Action of Pulp in Wet - Laid Non - Woven Processes

The paper deals with the fluid field of web forming in wet-laid non-woven production.The influence of the turbulent flow on blending fiber and occluded fluid produced in pulp flow has been discussed in theory and practice.The suitable use of the imported velocity of pulp is very important in producing wet-laid products of good quality.

CYFRA 21-1 as an early predictor of first line chemotherapy response in advanced non small cell lung cancer

Objective： In an era of ever evolving, promising new therapies for advanced non small cell lung cancer （NSCLC）, early predictors of response to therapy, are needed. We evaluated early variations in CYFRA 21-1 serum levels of patients with advanced NSCLC receiving first line chemotherapy and correlated the results with objective tumor response. Methods： 29 consecutive, previously untreated, patients of advanced non small cell lung cancer, with measurable disease on CT scan were evaluated. All patients were treated with conventional systemic chemotherapy, although the choice of chemotherapy was left to the discretion of the treating physicians. Serum samples were obtained immediately before the start of 1st and 2nd cycles of chemotherapy. CYFRA 21-1 was measured with an electrochemiluminescense immunoassay on an automatic analyzer （Elecsys 2000; Roche Diagnostics）. Response was evaluated using Response evaluation criteria in solid tumors （RECIST） criteria. Results： 10 patients had partial response, 9 patients had stable disease and 9 had progressive disease. None of the patients had complete response. 21/29 （72%） patients had an elevated baseline value of CYFRA 21-1.62% patients （18/29） had a decrease in CYFRA 21-1 after 1 cycle of chemotherapy. The average reduction in the 2nd reading was irrespective of whether baseline value was normal or not. The average reduction was statistically significant （P = 0.002; 95% CI, from 0.8369 to 3.49464; t test）. 8 out of 10 （80%） patients with partial response had a reduction in their 2nd reading of. CYFRA （P = 0.019; 95% CI, from 0.81965 to 7.20035; t test） which was significant. We also observed that 6/9 （66%） patients whose disease remains stable also had a decrease in their subsequent reading （P = 0.0106; 95% CI, from -0.44942 to 3.82720; t test）, though it was not significant statistically. Although 5 out of 9 （55%） patients, who had an increase in their CYFRA 21-1 level, had progressive disease, but it was not statistically significant （P = 0.537; 95% CI, from -1.20021 to 2.13354; ttest）. 14 out of 19 （73%） who either had partial response or had stable disease, had a reduction in their 2nd value of CYFRA 21-1 and was significant statistically （P = 0.004; 95% CI, from 0.74792 to 3.50208; t test）. We also observed that except for 1 patient, all patients who had a decrease of 42% or more in their subsequent CYFRA 21-1 level, were those who had either responded to chemotherapy or had stable disease （P = 0.001）, which was statistically significant. Conclusion： We can conclude that monitoring of serum marker CYFRA 21-1, early dudng first-line chemotherapy may be a useful prognostic tool for evaluation of early tumor response in patients with advanced NSCLC.

MiR-16-5p plays an inhibitory role in human non-small cell lung cancer through Fermitin family member 2

Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression.However,the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer(NSCLC)are not to be well studied.Here,we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines.The correlation between the clinicopathological features of NSCLC and the miR-16-5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage,positive lymph node metastasis,with short overall survival(OS).Also,a negative correlation between miR-16-5p and Fermitin family member 2(FERMT2)was observed,implying there may be a potential link about their regulation.The hypothesis was further confirmed by in-silico analysis and dual-luciferase reporter assay.Moreover,we demonstrated that the transfections of miR-16-5p mimics could alter some biological characteristics of NSCLC cells remarkably accomplished by the expression variance of FERMT2 in vitro and in vivo assays.Summarily,this study demonstrated that miR-16-5p,as a tumor suppression factor in NSCLC by targeting FERMT2,could serve as one promising biomarker in the prediction for NSCLC patients.

优势传统黄酒类制造业关键技术与应用系列-4 黄酒非糖固形物成分的研究

非糖固形物是黄酒国家标准中的控制指标,也是黄酒生产企业普遍关注的一个问题。本文通过对其剖析表明,不同生产工艺酒样的非糖固形物成分一致,为糊精、蛋白质类物质,有机酸,无机元素和酚类物质等。其中糊精和蛋白类物质是非糖固形物中的两个最重要组分,两者主要来自酶对原料中淀粉和蛋白质的降解。不同工艺黄酒中非糖固形物成分的含量差异显著,主要与三个因素密切相关:工艺、原料和测定方法。

N-(2-氯-4-吡啶基)-N'-苯基脲(CPPU)对甜柿果实中淀粉、还原糖含量和淀粉酶活性的影响

柿子品种‘卫郎’和‘禅寺丸’花后10d的果实喷施N-(2-氯-4-吡啶基)-N'-苯基脲(CPPU)后,果实中的还原糖含量增加,淀粉含量减少,淀粉酶活性显著提高。

不完全消解-高分辨连续光源石墨炉原子吸收光谱法测定蔗糖中镉和铅

建立了不完全消解-高分辨连续光源石墨炉原子吸收光谱法测定蔗糖中Cd,Pb的方法。试验选择不完全消解作为样品的前处理方式,比较了几种常用的基体改进剂,发现磷酸二氢铵的影响显著,并且对吸收峰的峰形有显著改善。测定蔗糖中Cd,Pb分别为2.75,107.3ng/g,检出限分别为0.073,0.935ng/mL,加标回收率分别为94.3%,96.2%,本法的精密度(RSD)均小于4.0%。该方法快速、准确、稳定、污染少,具有较高的实用价值,为食品中多元素检测提供了科学依据。

发芽小麦α-淀粉酶活性的研究

通过对在不同发芽温度和不同发芽时间下培养的芽麦粉中α 淀粉酶活性、降落值、还原糖及非还原糖含量的测定 ,研究了发芽小麦的α 淀粉酶活性、降落值、还原糖及非原糖含量与芽麦的发芽温度和发芽时间的关系。并通过响应面分析法 ,进行中心组合设计 ,得出了它们的二元二次回归方程。并对植酸作为α 淀粉酶抑制剂的抑制效果进行了研究 .通过测定不同植酸添加量下的α 淀粉酶活性及降落值 ,表明植酸对α 淀粉酶的抑制效果十分明显 。

紫杉醇联合顺铂对晚期NSCLC患者的疗效及血清CEA、CA125、Cyfra21-1及NSE水平的影响

目的探讨紫杉醇联合顺铂对晚期非小细胞肺癌(NSCLC)患者的疗效及血清癌坯抗原(CEA)、糖抗原125(CA125)、细胞角质素片段抗原21-1(Cyfra21-1)、神经元特异性烯醇化酶(NSE)水平的影响。方法选取2012年1月至2016年1月确诊的晚期NSCLC患者120例,根据化疗方案分为A组60例(紫杉醇联合顺铂)、B组60例(吉西他滨联合顺铂),比较2组的化疗效果。结果 A组的总缓解率为45. 00%、总有效率为95. 00%与B组的40. 00%、88. 33%比较,差异均无统计学意义(P> 0. 05);治疗前后,2组患者血清CEA、CA125、Cyfra21-1、NSE水平差异无统计学意义(P> 0. 05);治疗后,2组患者的血清CEA、CA125、Cyfra21-1、NSE水平较本组治疗前均显著降低(P <0. 05); A组患者的恶心呕吐、腹泻、白细胞减少、血小板降低、血红蛋白降低程度显著的低于B组(P <0. 05); 2组患者的皮疹、肝肾功能损害、脱发程度比较差异无统计学意义(P> 0. 05);随访1年,A组失访3例,生存23例(40. 35%),B组失访5例,生存20例(36. 36%),2组1年生存率比较差异无统计学意义(P> 0. 05)。结论紫杉醇联合顺铂与吉西他滨联合顺铂治疗NSCLC的效果相当,但是不良反应发生率较低。

基于SiPLS-CARS算法的百香果糖度无损检测

为建立一种无损快速检测百香果糖度的技术,以百香果为研究对象,利用近红外光谱技术,并结合联合区间偏最小二乘算法和竞争适应重加权采样算法对近红外光谱进行特征波长筛选,采用偏最小二乘法和支持向量机方法建立百香果糖度预测模型。结果表明:采用多元线性回归方法建立的模型优于多元非线性回归方法建立的模型,联合区间偏最小二乘算法和竞争适应重加权采样算法筛选出的特征波长点数为67个,占全光谱的2.90%,预测模型的相关系数R2c为0.9727,校正集预测均方根误差(root mean square error of calibration,RMSEC)值为0.3338,验证集的相关系数R2p为0.9672,验证集预测均方根误差(root mean square error of prediction,RMSEP)值为0.3660,模型相对分析误差(relative prediction deviation,RPD)为4.5066。研究结果能够实现百香果糖度的无损快速检测,并且可以将百香果糖度无损检测便携检设备中的模型进行简化。

基于体外仿生消化法筛选适用于肉鸡玉米-豆粕-杂粕型饲粮的非淀粉多糖复合酶

本试验旨在探究响应面法优化筛选6种非淀粉多糖酶(木聚糖酶、β-葡聚糖酶、纤维素酶、β-甘露聚糖酶、α-半乳糖苷酶、果胶酶)添加于肉鸡玉米-豆粕-杂粕型饲粮中最优组合酶谱。采用第3代单胃动物仿生消化系统(SDS-Ⅲ)进行模拟胃肠液体外消化试验,首先采用单因素完全随机试验设计,在肉鸡玉米-豆粕-杂粕型基础饲粮中分别添加5个水平的6种非淀粉多糖酶,每个水平设5个重复,以还原糖释放量(RS)和干物质消化率提高值(IDMD)为评价指标,确定单酶的最佳添加量;据此结果,进一步利用软件Design-Expert 8.06 Box-Behnken响应面法设计6因子3水平L 54(36)试验,对6种单酶进行复配组合,以RS和IDMD为响应值,确定6种单酶的最佳组合酶谱。结果表明:在1~3周龄肉鸡玉米-豆粕-杂粕型饲粮中优选出的6种非淀粉多糖酶酶谱为木聚糖酶11.40 U/g、β-葡聚糖酶3.76 U/g、纤维素酶8.52 U/g、β-甘露聚糖酶8.19 U/g、α-半乳糖苷酶6.24 U/g、果胶酶1.60 U/g,该酶谱催化反应的RS和IDMD分别为9.71 mg/g和2.86%;在此条件下进行3次重复试验,得到RS和IDMD分别为9.59 mg/g和2.81%,与理论最优值的误差分别为1.24%和1.75%,表明所得酶谱能反映出对RS和IDMD的较好结果。在4~6周龄肉鸡玉米-豆粕-杂粕型饲粮中优选出的6种非淀粉多糖酶酶谱为木聚糖酶11.90 U/g、β-葡聚糖酶5.26 U/g、纤维素酶8.32 U/g、β-甘露聚糖酶7.96 U/g、α-半乳糖苷酶6.29 U/g、果胶酶6.17 U/g,该酶谱催化反应的RS和IDMD分别为10.45 mg/g和2.95%;在此条件下进行3次重复试验,得到RS和IDMD分别为10.34 mg/g和2.92%,与理论最优值的误差分别为1.05%和1.02%,表明所得酶谱能反映出对RS和IDMD的较好结果。综上所述,1~3周龄肉鸡饲粮6种非淀粉多糖酶最佳酶谱是木聚糖酶11.40 U/g、β-葡聚糖酶3.76 U/g、纤维素酶8.52 U/g、β-甘露聚糖酶8.19 U/g、α-半乳糖苷酶6.24 U/g、果胶酶1.60 U/g;4~6周龄肉鸡饲粮6种非淀粉多糖酶最佳酶谱是木聚糖酶11.90 U/g、β-葡聚糖酶5.26 U/g、纤维素酶8.32 U/g、β-甘露聚糖酶7.96 U/g、α-半乳糖苷酶6.29 U/g、果胶酶6.17 U/g。

FAREY SEQUENCE OF NONLINEAR DIFFERENTIAL DYNAMIC SYSTEM IN TWO DIMENSIONS

In this paper , through the discrim ination of Farey sequence in the forced Brusselator withweak coupling , it is proved that there is a topological translation fro m a nonlinear differen tial system ( limit cycle) to the circle m ap .

β-木糖苷酶型非淀粉多糖酶对小麦的体外酶解及消化研究

以灭活内源酶的小麦为原料,测定添加β-木糖苷酶和其他非淀粉多糖(NSP)酶后小麦的分解效果,并采用单胃动物仿生消化系统模拟猪的胃肠道消化过程,观察小麦中添加β-木糖苷酶型NSP酶消化率的变化。结果表明,分解小麦产生还原糖效果以β-木糖苷酶和NSP酶组最佳,β-木糖苷酶和木聚糖酶组次之,单独添加木聚糖酶组效果最差;当β-木糖苷酶添加量达到16U/g小麦时,与NSP酶结合产出的还原糖量可达到最高,为28.16mg/g,与空白不加酶组相比,还原糖提升68.52%,酶解效果显著。模拟动物胃肠道消化过程也发现,NSP酶中加入β-木糖苷酶可使小麦干物质消化率和能量消化率进一步增长0.4%和0.5%左右,但这种提高与β-木糖苷酶剂量高低无关。

粮食中还原糖和非还原糖测定国家标准(GB/T5513-2008)中存在问题的修改建议

在依据国标(GB/T 5513-2008)进行还原糖和非还原糖含量的测定过程中,发现该标准有些地方表达不太准确或缺乏可操作性。该文针对还原糖和非还原糖测定测定中遇到的问题,指出了该标准中的不足并提出相应的修改建议。

Ways to Overcome Anxiety in the English Listening Class in College (SUN JING-YU KE SU-FEN)

In my ten-yeat experience of tenching English as major in college , I feel that students experience more difficulties in listening lessons than in other lessons. What, then, prevents students from achieving success in the listening class? I suggest that anxiety is one of the major factors, which interferes with students listening comprehension. And I would agree with writers such as Gardner and Horwitz et al. (1991)that anxiety may impede learner’s ability to perform successfully in all aspects of learning a foreign language. I feet it is particularly significant for listening.

柯里拉京调控AMPK-自噬信号改善高脂高果糖饮食诱导的小鼠非酒精性脂肪肝病

目的探究柯里拉京通过调控AMPK-自噬信号对高脂高果糖饮食诱导的小鼠非酒精性脂肪肝病的影响。方法健康雄性8周龄C57BL/6J小鼠随机分为对照组、模型组和柯里拉京给药组,其中模型组和柯里拉京给药组小鼠于8周龄开始给予高脂高果糖饮食饲养4周,柯里拉京给药组小鼠同时腹腔注射柯里拉京20 mg·kg-1,隔天给药1次,连续给药4周,对照组和模型组给予等剂量生理盐水。造模和给药结束后小鼠处死并记录其肝质量,HE染色、油红O染色、Masson染色观察肝组织病理学特征,试剂盒检测血清和肝脏组织中生化指标,Western blot检测肝脏自噬和p-AMPK水平。结果实验结果发现,模型组小鼠肝质量增加,血清中AST、ALT明显升高,肝脏中出现了大量的脂肪空泡和严重的脂质沉积并有轻度的胶原纤维增生,肝脏中TG水平明显升高,柯里拉京干预后小鼠肝质量降低,肝脏病理改变得到明显改善,TG水平降低;Western blot结果发现,模型组肝脏中Atg7和Atg5等自噬相关蛋白水平明显降低,p-AMPK水平也明显降低,柯里拉京干预后明显升高p-AMPK,并上调自噬水平。结论柯里拉京可以改善高脂高果糖饮食诱导的小鼠非酒精性脂肪肝病,其机制可能是通过促进AMPK磷酸化进而上调自噬水平。

云南小碗红糖非遗品牌发展路径研究

随着农业的发展,农产品品牌化对推进乡村振兴和农业农村现代化发挥着重要作用。本文以云南省巧家县红糖产业为研究对象,通过实地调研和网络调研相结合的方法,总结巧家县红糖产业发展的机遇与优势,发现巧家县小碗红糖品牌发展存在品牌价值缺位、产品附加值不高、发展合力不足、培育机制不完善等问题。为此,提出丰富品牌多元要素、优化生产质量标准、壮大本土经营主体、明晰品牌培育机制等策略,为巧家小碗红糖品牌建设进一步完善提供建议与参考。