Monitoring subtle changes in ionic current flow through a nanopore could be applied to observe single molecule reaction. Here,we introduced cysteine to substitute for lysine at position 238 constructing a mutant aerol...Monitoring subtle changes in ionic current flow through a nanopore could be applied to observe single molecule reaction. Here,we introduced cysteine to substitute for lysine at position 238 constructing a mutant aerolysin K238 C. It could be regarded as a nanoreactor to efficiently visualize chemical bonds making and breaking. The compound 5,5′-dithiobis-(2-nitrobenzoic acid)(DTNB) was selected as a reactant coming into collisions on the interface of the pore to occur a reversible reaction. Our results showed that the mutant aerolysin could respond to three molecules of DTNB simultaneously and reflect corresponding levels with distinguishable current signals. Therefore, this method constitutes a simple, generic tool for monitoring single molecule reaction, which evokes a guidance for the mutant aerolysin towards the application of tracking other more reactions at single molecule level.展开更多
针对φ73.0 mm×5.51 mm N80 EU TBG油管粘扣和脱扣事故进行了深入调查研究,对粘扣和脱扣的油管样品进行了宏观分析;对该批油管螺纹接头加工质量进行了全面检测;对油管材质进行了理化试验;对油管上、卸扣性能和拉伸性能进行了系统...针对φ73.0 mm×5.51 mm N80 EU TBG油管粘扣和脱扣事故进行了深入调查研究,对粘扣和脱扣的油管样品进行了宏观分析;对该批油管螺纹接头加工质量进行了全面检测;对油管材质进行了理化试验;对油管上、卸扣性能和拉伸性能进行了系统试验。通过试验分析和研究,认为油管螺纹加工质量和材质符合标准,油管脱扣是由于粘扣后接头连接强度降低所致,油管粘扣是操作不当引起的。展开更多
基金supported by the National Natural Science Foundation of China (21421004, 21777041, 21327807)the Program of Introducing Talents of Discipline to Universities (B16017)+1 种基金Innovation Program of Shanghai Municipal Education Commission (2017-0107-00-02-E00023)the Fundamental Research Funds for the Central Universities (222201718001, 222201717003)
文摘Monitoring subtle changes in ionic current flow through a nanopore could be applied to observe single molecule reaction. Here,we introduced cysteine to substitute for lysine at position 238 constructing a mutant aerolysin K238 C. It could be regarded as a nanoreactor to efficiently visualize chemical bonds making and breaking. The compound 5,5′-dithiobis-(2-nitrobenzoic acid)(DTNB) was selected as a reactant coming into collisions on the interface of the pore to occur a reversible reaction. Our results showed that the mutant aerolysin could respond to three molecules of DTNB simultaneously and reflect corresponding levels with distinguishable current signals. Therefore, this method constitutes a simple, generic tool for monitoring single molecule reaction, which evokes a guidance for the mutant aerolysin towards the application of tracking other more reactions at single molecule level.
文摘针对φ73.0 mm×5.51 mm N80 EU TBG油管粘扣和脱扣事故进行了深入调查研究,对粘扣和脱扣的油管样品进行了宏观分析;对该批油管螺纹接头加工质量进行了全面检测;对油管材质进行了理化试验;对油管上、卸扣性能和拉伸性能进行了系统试验。通过试验分析和研究,认为油管螺纹加工质量和材质符合标准,油管脱扣是由于粘扣后接头连接强度降低所致,油管粘扣是操作不当引起的。
