【目的】为阐明哺乳动物雷帕霉素靶点(mammalian target of Rapamycin,mTOR)信号通路在调控猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)复制过程中的作用,探索防控该病传染的新途径。【方法】P...【目的】为阐明哺乳动物雷帕霉素靶点(mammalian target of Rapamycin,mTOR)信号通路在调控猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)复制过程中的作用,探索防控该病传染的新途径。【方法】PRRSV感染猪肺泡巨噬细胞(porcine alveolar macrophage,PAMs)24 h后,用蛋白质印迹法检测mTOR信号通路下游信号分子的表达水平及磷酸化水平。【结果】在PRRSV感染PAMs后,mTOR、eIF4E结合蛋白1(eukaryotic initiation factor 4E binding protein 1,4EBP1)及PS6K的磷酸化水平显著增加,表明PRRSV感染激活了mTOR信号通路。用mTOR特异性抑制剂雷帕霉素(Rapamycin)处理PAMs,发现Rapamycin能够显著抑制PRRSV感染引起的mTOR信号通路下游关键信号分子4EBP1磷酸化,且剂量依赖性和时间依赖性促进了PRRSV的复制。【结论】mTOR信号通路参与调控PRRSV的复制,Rapamycin对4EBP1磷酸化有抑制作用,对PRRSV的复制有促进作用。该研究为后续进一步探索PRRSV复制的翻译起始机制奠定基础。展开更多
4-ary pulse amplitude modulation(4PAM) signals with 33% and 50% return-to-zero(RZ) clocks are generated for passive optical network(PON). We demonstrate that RZ-4PAM signals with duty cycles of 33% and 50% after trans...4-ary pulse amplitude modulation(4PAM) signals with 33% and 50% return-to-zero(RZ) clocks are generated for passive optical network(PON). We demonstrate that RZ-4PAM signals with duty cycles of 33% and 50% after transmission over 20-km-long single mode fiber(SMF) at 10 Gbit/s can be directly detected by using one photo detector,and the original data can also be restored by one M-ary threshold detector and one 4PAM sequence decoder. The optical spectra of 33% and 50% RZ-4PAM signals are measured,and their eye-diagrams before and after transmission are also analyzed. Simulation results show that 33% and 50% RZ-4PAM downlink signals can be received effectively,and the received power values are-15.1 d Bm and-13.8 d Bm when the bit error rate(BER) is 10^(-6). Moreover,33% RZ-4PAM optical signals have better reception performance than 50% RZ-4PAM optical signals.展开更多
We report 20 Gb/s transmission of four-level pulse amplitude modulation （PAM） signal using a directly modulated tunable distributed Bragg reflector （DBR） laser. Transmission distance over 20 km was achieved withou...We report 20 Gb/s transmission of four-level pulse amplitude modulation （PAM） signal using a directly modulated tunable distributed Bragg reflector （DBR） laser. Transmission distance over 20 km was achieved without using optical amplifiers and optical dispersion compensation modules. A wavelength tuning range of 11.5 nm and a 3 dB bandwidth greater than 10 GHz over the entire wavelength tuning range were obtained.展开更多
文摘【目的】为阐明哺乳动物雷帕霉素靶点(mammalian target of Rapamycin,mTOR)信号通路在调控猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)复制过程中的作用,探索防控该病传染的新途径。【方法】PRRSV感染猪肺泡巨噬细胞(porcine alveolar macrophage,PAMs)24 h后,用蛋白质印迹法检测mTOR信号通路下游信号分子的表达水平及磷酸化水平。【结果】在PRRSV感染PAMs后,mTOR、eIF4E结合蛋白1(eukaryotic initiation factor 4E binding protein 1,4EBP1)及PS6K的磷酸化水平显著增加,表明PRRSV感染激活了mTOR信号通路。用mTOR特异性抑制剂雷帕霉素(Rapamycin)处理PAMs,发现Rapamycin能够显著抑制PRRSV感染引起的mTOR信号通路下游关键信号分子4EBP1磷酸化,且剂量依赖性和时间依赖性促进了PRRSV的复制。【结论】mTOR信号通路参与调控PRRSV的复制,Rapamycin对4EBP1磷酸化有抑制作用,对PRRSV的复制有促进作用。该研究为后续进一步探索PRRSV复制的翻译起始机制奠定基础。
基金supported by the National Natural Science Foundation of China(No.61107064)the Chongqing University Innovation Team Founding(No.KJTD201320)+3 种基金the Natural Science Foundation of Chongqing Science & Technology Commission(Nos.cstc2014jcyj A40046 and cstc2016jcyj A1233)the Science and Technology Special Funds of Wanzhou District in Chongqing Citythe Chongqing Graduate Student Research Innovation Project(No.CYS15225)the Chongqing Three Gorges University Graduate Scientific Research Innovation Project
文摘4-ary pulse amplitude modulation(4PAM) signals with 33% and 50% return-to-zero(RZ) clocks are generated for passive optical network(PON). We demonstrate that RZ-4PAM signals with duty cycles of 33% and 50% after transmission over 20-km-long single mode fiber(SMF) at 10 Gbit/s can be directly detected by using one photo detector,and the original data can also be restored by one M-ary threshold detector and one 4PAM sequence decoder. The optical spectra of 33% and 50% RZ-4PAM signals are measured,and their eye-diagrams before and after transmission are also analyzed. Simulation results show that 33% and 50% RZ-4PAM downlink signals can be received effectively,and the received power values are-15.1 d Bm and-13.8 d Bm when the bit error rate(BER) is 10^(-6). Moreover,33% RZ-4PAM optical signals have better reception performance than 50% RZ-4PAM optical signals.
基金supported by the National Key Research and Development Program of China(Nos.2016YFB0402301 and 2017YFF0206103)the National Natural Science Foundation of China(Nos.61320106013,61635010,61474112,61574137,and 61504170)
文摘We report 20 Gb/s transmission of four-level pulse amplitude modulation （PAM） signal using a directly modulated tunable distributed Bragg reflector （DBR） laser. Transmission distance over 20 km was achieved without using optical amplifiers and optical dispersion compensation modules. A wavelength tuning range of 11.5 nm and a 3 dB bandwidth greater than 10 GHz over the entire wavelength tuning range were obtained.
