Non-enzymatic methods for isolation of stromal vascular fraction and adipose-derived stem cells:A systematic review

BACKGROUND Adipose-derived stem cells(ADSCs)and the stromal vascular fraction(SVF)have garnered substantial interest in regenerative medicine due to their potential to treat a wide range of conditions.Traditional enzymatic methods for isolating these cells face challenges such as high costs,lengthy processing time,and regulatory complexities.AIM This systematic review aimed to assess the efficacy and practicality of nonenzymatic,mechanical methods for isolating SVF and ADSCs,comparing these to conventional enzymatic approaches.METHODS Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines,a comprehensive literature search was conducted across multiple databases.Studies were selected based on inclusion criteria focused on non-enzymatic isolation methods for SVF and ADSCs from adipose tissue.The risk of bias was assessed,and a qualitative synthesis of findings was performed due to the methodological heterogeneity of the included studies.RESULTS Nineteen studies met the inclusion criteria,highlighting various mechanical techniques such as centrifugation,vortexing,and ultrasonic cavitation.The review identified significant variability in cell yield and viability,and the integrity of isolated cells across different non-enzymatic methods compared to enzymatic procedures.Despite some advantages of mechanical methods,including reduced processing time and avoidance of enzymatic reagents,the evidence suggests a need for optimization to match the cell quality and therapeutic efficacy achievable with enzymatic isolation.CONCLUSION Non-enzymatic,mechanical methods offer a promising alternative to enzymatic isolation of SVF and ADSCs,potentially simplifying the isolation process and reducing regulatory hurdles.However,further research is necessary to standardize these techniques and ensure consistent,high-quality cell yields for clinical applications.The development of efficient,safe,and reproducible non-enzymatic isolation methods could significantly advance the field of regenerative medicine.

The Non-Enzymatic Antioxidant and Level of Oxidative Stress of Tuberculosis Patients in Selected Treatment Center in Addis Ababa Ethiopia

Introduction: Non-enzymatic antioxidants are good scavengers of free radicals preventing their overproduction there by reducing the level of oxidative stress. This work was undertaken at Saint Peter TB specialized hospital and TekleHaimanot health center from March 2012 to May 2013.Aim: To determine changes in Non-Enzymatic Antioxidants and level of oxidative stress of tuberculosis Patients before and after taking anti tuberculosis treatment.Materials and Methods: In this comparative cross sectional study, a total of 210 individuals including: newly diagnosed TB patients as group-I (n = 70), TB patients who completed treatment as group-II (n = 70), and healthy volunteers as group-III (n = 70) were enrolled. Different methods were used to determine the parameters;vit-C (HPLC method), lipid peroxidation (thiobarbuituric acid method), and bilirubin (Colorimetric assay). Results: Vitamin-C (Vit-C) and of group-I showed a significant reduction (p < 0.001) as compared with both group-II and group-III whereas Malondialdehyde (MDA) level was increased. However, the total and direct bilirubin was not different among the groups. In group-III, there was a positive correlation between BMI and serum Vit-C (r = -0.305, p = 0.010). Vit-C showed a negative correlation with serum MDA in all the groups with values (r = -0.265, p = 0.027), (r = -0.389, p = 0.001) and (r = -0.375, p = 0.001) for group-I, group-II and group-III respectively. In addition to this Vit-C was negatively correlated with serum UA (r = -0.285, p = 0.017) in group-I. Conclusion: The findings of the current study suggest that the amount of Vit-C in the newly diagnosed TB patients and those who finished their treatment is much lower than the healthy volunteers. In contrast to this, the MDA value was significantly higher both in the newly diagnosed TB patients and TB patients who completed treatment than in healthy volunteers suggesting higher degree of oxidative stress.

A CuNi/C Nanosheet Array Based on a Metal–Organic Framework Derivate as a Supersensitive Non-Enzymatic Glucose Sensor

Bimetal catalysts are good alternatives for nonenzymatic glucose sensors owing to their low cost, high activity, good conductivity, and ease of fabrication. In the present study, a self-supported CuNi/C electrode prepared by electrodepositing Cu nanoparticles on a Ni-based metal–organic framework(MOF) derivate was used as a non-enzymatic glucose sensor. The porous construction and carbon scaffold inherited from the Ni-MOF guarantee good kinetics of the electrode process in electrochemical glucose detection. Furthermore, Cu nanoparticles disturb the array structure of MOF derived films and evidently enhance their electrochemical performances in glucose detection. Electrochemical measurements indicate that the CuNi/C electrode possesses a high sensitivity of17.12 mA mM^(-1) cm^(-2), a low detection limit of 66.67 nM,and a wider linearity range from 0.20 to 2.72 mM. Additionally, the electrode exhibits good reusability, reproducibility, and stability, thereby catering to the practical use of glucose sensors. Similar values of glucose concentrations in human blood serum samples are detected with our electrode and with the method involving glucose-6-phosphate dehydrogenase; the results further demonstrate the practical feasibility of our electrode.

A novel sensitive non-enzymatic glucose sensor

Cu nanoclusters were electrochemically deposited on the film of a Nafion-solubilized multi-wall carbon nanotubes (CNTs) modified glassy carbon electrode (CNTs-GCE), which fabricated a Cu-CNTs composite sensor (Cu-CNTs-GCE) to detect glucose with non-enzyme. The linear range is 7.0 × 10?7 to 3.5 × 10?3 mol/L with a high sensitivity of 17.76 μA/(mmol L), with a low detection limit 2.1 × 10?7 mol/L, fast response time (within 5 s), good reproducibility and stability.

Novel insights into conjugation of antitumor-active unsymmetrical bisacridine C-2028 with glutathione:Characteristics of non-enzymatic and glutathione S-transferase-mediated reactions

Unsymmetrical bisacridines(UAs) are a novel potent class of antitumor-active therapeutics.A significant route of phase II drug metabolism is conjugation with glutathione(GSH),which can be non-enzymatic and/or catalyzed by GSH-dependent enzymes.The aim of this work was to investigate the GSHmediated metabolic pathway of a representative UA,C-2028.GSH-supplemented incubations of C-2028 with rat,but not with human,liver cytosol led to the formation of a single GSH-related metabolite.Interestingly,it was also revealed with rat liver microsomes.Its formation was NADPH-independent and was not inhibited by co-incubation with the cytochrome P450(CYP450) inhibitor 1-aminobenzotriazole.Therefore,the direct conjugation pathway occurred without the prior CYP450-catalyzed bioactivation of the substrate.In turn,incubations of C-2028 and GSH with human recombinant glutathione S-transferase(GST) P1-1 or with heat-/ethacrynic acid-inactivated liver cytosolic enzymes resulted in the presence or lack of GSH conjugated form,respectively.These findings proved the necessary participation of GST in the initial activation of the GSH thiol group to enable a nucleophilic attack on the substrate molecule.Another C-2028-GSH S-conjugate was also formed during non-enzymatic reaction.Both GSH S-conjugates were characterized by combined liquid chromatography/tandem mass spectrometry.Mechanisms for their formation were proposed.The ability of C-2028 to GST-mediated and/or direct GSH conjugation is suspected to be clinically important.This may affect the patient’s drug clearance due to GST activity,loss of GSH,or the interactions with GSH-conjugated drugs.Moreover,GST-mediated depletion of cellular GSH may increase tumor cell exposure to reactive products of UA metabolic transformations.

Non-Enzymatic Glucose Sensor Based on the Novel Flower Like Morphology of Nickel Oxide

In this study, novel nickel oxide (NiO) flowers like nanostructures were fabricated onto gold coated glass substrate by hydrothermal method using high alkaline pH medium. The structural study of nickel oxide nanostructures was performed by scanning electron microscopy (SEM) and X-ray differaction (XRD) techniques. Nickel oxide nanostructures are highly dense, uniform and possess good crystalline quality. The so prepared structures were investigated for their electrochemical properties by cyclic voltammetry and amperometric techniques. The nickel oxide flower like morphology has shown good electrochemical performances for the oxidation of glucose. The presented sensing material was able to detected glucose in a wide range of concentration of 0.001 mM to 8 mM with a high sensitivity (123 μmA/mM) and regression coefficient of 0.99. Moreover, the NiO nanostructures based sensor is highly reproducible, stable, exhibiting a fast response time and selective in the response. All the obtained results indicate the potential use of this material in the development of enzyme free sensors for the detection of glucose.

3种来源黑木耳黑色素的性质及其体外抗氧化活性比较

该研究以黑木耳“1703”的子实体、菌丝体和发酵液为试验材料,采用碱溶酸沉法提取黑色素,采用紫外可见光谱(ultraviolet–visible spectroscopy,UV-Vis)、傅里叶变换红外光谱(Fourier transform infrared spectrometer,FTIR)、扫描电子显微镜(scanning electron microscope,SEM)、高效液相色谱(high performance liquid chromatography,HPLC)法研究黑色素的结构表征,测定黑木耳黑色素的溶解性、稳定性和体外抗氧化活性。UV-Vis结果表明3种来源黑木耳黑色素最大吸收波长均为210 nm。FTIR结果表明3种来源黑木耳黑色素均具有天然黑色素的3个特征吸收区。HPLC结果表明3种来源黑木耳黑色素均只有一个吸收峰,且保留时间基本一致。溶解性结果表明3种来源黑木耳黑色素均易溶于碱性溶液,微溶于75%乙醇溶液,难溶于水和多数有机溶剂。稳定性结果表明3种来源黑木耳黑色素均具有良好的温度稳定性和光照稳定性,Fe^(3+)能够促进黑色素溶解,Fe^(2+)和Cu^(2+)导致黑色素不稳定。体外抗氧化试验结果表明3种来源黑木耳黑色素对DPPH自由基、ABTS^(+)自由基和羟基自由基均具有较强的清除能力,其中发酵液黑色素清除能力最强。

The Impact of Microbial Transglutaminase on the Quality and Antioxidant Activity of Camel-Milk Soft Cheese

This study aimed at investigating the impact of adding microbial transglutaminase (MTGase) after rennet addition on some properties of fresh soft cheese made from camel milk. MTGase was added to milk at concentration of 80, 100 and 120 U/L after 20 and 30 min of renneting. The chemical composition, yield, hardness, antioxidant activity and sensory properties of cheese were estimated. Enzymatic protein crosslinking was analyzed by SDS-PAGE. Results revealed that MTGase-treated cheeses were higher in moisture and lower in protein content compared to control. In addition, the concentration of MTGase and time of addition significantly (P 0.05) impacted these parameters. Among treated cheeses, samples with 80 U of MTGase and addition time of 20 min were the highest in total solids and protein content. Adding MTGase significantly (P 0.05) increased the cheese yield, however, increased MTGase concentration at any time of addition did not improve it. The electrophoretic patterns of MTGase-cheese proteins showed a reduction in the intensity of caseins bands and the appearance of new protein fractions with high molecular weights. However, the changes in the intensity of the whey proteins bands were not sufficiently clear as caseins. The cheese hardness was significantly (P 0.05) affected by adding MTGase. Cheese containing 80 U of MTGase had the highest hardness value compared to control and other treated samples. The antioxidant activity of cheese was negatively influenced by adding the enzyme. The use of MTGase enhanced the mouthfeel, texture and overall acceptability of cheese. However, the effect of MTGase concentration and addition time was not significant (P > 0.05) on the sensory attributes. In conclusion, adding MTGase to milk at concentration of 80 U after 20 min of renneting is recommended to improve the yield, textural and some sensory properties of fresh soft cheese made from camel milk.

Oxidative Stress and Antioxidant Status in Acute and Chronic Myeloid Leukemia Patients

Oxidative stress, a pervasive condition of increased number of reactive oxygen species, is now recognized to be prominent feature of various diseases and their progression. The relationship between antioxidants and levels of well-known markers of oxidative stress, measured as lipid peroxides and oxidized proteins reflect health indices. The aim of this study is to evaluate the extent of oxidative stress and antioxidant status in acute and chronic myeloid leukemia patients. The present study included 60 patients selected using standard questionnaire based on age, family history, Body Mass Index (BMI), dietary intake, with no other complications and 30 age and sex-matched healthy subjects. The median age of myeloid leukemia patients was 43 years and that of controls was 42 years. Out of 60 myeloid leukemia patients, 30 were in acute and 30 were in chronic state. Oxidative stress and antioxidant status were evaluated in the patients and in the controls by assessing standard oxidative stress markers viz. plasma and erythrocyte lipid peroxide levels in terms of malondialdehyde and oxidized proteins as protein carbonyls whereas antioxidant status was assessed in terms of serum non enzymatic antioxidant levels. There was a significant increase (p 0.01) in plasma and erythrocyte lipid peroxidation and protein oxidation in acute and chronic myeloid leukemia patients as compared to healthy subjects. Antioxidant status as indicated by the levels of non-enzymatic antioxidants viz. erythrocyte reduced glutathione (GSH), serum β carotene, vitamin A & C and ceruloplasmin was found to be significantly decreased (p 0.01) in both the leukemia patients as compared to healthy participants. However, chronic myeloid leukemia patients had significantly (p 0.05) higher oxidative stress and lower antioxidant status as compared to acute myeloid leukemia patients.

针刺治疗对2型糖尿病周围神经病变患者氧化应激水平的研究

目的评价整合针灸疗法对糖尿病周围神经病变(diabetic peripheral neuropathy,DPN)痰瘀互结证患者的临床疗效。方法选择2022年3月—2023年6月在海南省中医院康复科及内分泌科治疗的106个糖尿病周围神经病变患者纳入本次研究,按随机数表法进行分组,对照组和观察组各53例。两组患者均按照现行指南中的治疗方案给予常规治疗,包括控制血糖、营养神经等。观察组在此基础上增加整合针灸疗法,即针刺、艾灸、刺络及穴位埋针等中医特色疗法。观察两组患者治疗前后多伦多临床评分系统积分变化和氧化应激指标的变化。结果治疗后两组患者多伦多临床评分系统积分各项积分均较治疗前明显改善,且观察组的症状评分、感觉检测评分优于对照组,差异有统计学意义(P<0.05)。治疗后两组患者MDA、T-AOC均较治疗前下降,且观察组下降幅度大于对照组,差异有统计学意义(P<0.05)。两组SOD均较治疗前上升,差异有统计学意义(P<0.05)。结论在常规治疗基础上增加整合针灸疗法,可以更加有效地改善DPN痰瘀互结证患者的中医临床症状,其机制可能是通过抑制氧化应激实现的。

鲭鱼酶解工艺双响应面法优化及抗氧化活性研究

采用双响应面法优化超声波辅助酶解鲭鱼蛋白,制备鲭鱼多肽,探究其抗氧化活性.以酶解液对DPPH自由基和ABTS自由基的清除率为指标,获得最适蛋白酶并双响应面优化酶解工艺条件,探究其抗氧化活性.结果表明:木瓜蛋白酶为最佳水解酶,最优酶解工艺为pH 6.0、超声时间40 min、温度53℃、料液比1∶3(g/mL)、酶添加量3 000 U/g、酶解时间2 h,所得的酶解液DPPH自由基清除率为91.21%,ABTS自由基清除率为84.12%;鲭鱼多肽具有较好的抗氧化活性,ABTS自由基和DPPH自由基半抑制率IC50分别为32.75μg/mL、1.20 mg/mL.该研究结果可为鲭鱼抗氧化肽的大量制备提供工艺参考,为鲭鱼抗氧化多肽在食品工业中的应用研发提供了理论基础.

茭白提取物及不同溶剂萃取物的抗氧化活性研究

以茭白干和鲜茭白为原料,采用溶剂法得到茭白醇提物和水提物,分别用石油醚、乙酸乙酯、正丁醇溶剂对茭白醇提物进行萃取,测定茭白提取物及各溶剂萃取物中总多酚、总黄酮和总多糖含量,并分析其对DPPH、ABTS、羟基自由基的清除活性和总还原能力。结果表明:乙酸乙酯萃取物总多酚和总黄酮含量最高,鲜茭白醇提物总多糖含量最高;茭白提取物及各溶剂萃取物均检测出抗氧化活性,乙酸乙酯和正丁醇萃取物相对活性更高,可作为天然抗氧化剂;总多酚、总多糖含量与DPPH、羟基自由基清除能力呈极显著相关(P<0.01)。

Ce(Ⅲ)-modulation over non-enzymatic Pt/CeO_(2)/GO biosensor with outstanding sensitivity and stability for lactic acid detection

A series of non-enzymatic graphene functionalized biosensors was developed via deposition precipitation method for lactic acid(LA) detection,which we re characterized by transmission electron micro scopy(TEM),Raman spectroscopy,X-ray photoelectron spectroscopy(XPS),gas chromatography-mass spectrometry,liquid chromatography-mass spectro metry,and proton nuclear magnetic re sonance(~1H NMR).The electrochemical performances of the non-enzymatic biosensors were measured by means of the electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV) method.The comprehensive analysis of structures shows that Pt,CeO_(2),and GO components interact with each other.During the storing and releasing oxygen,the valence ratio of Ce^(3+)/Ce^(4+) and the number of oxygen vacancies in CeO_(2) change accordingly,which can be conducive to increasing electronic transmission capacity and finally leads to the improvement of electrocatalytic performance.Among them,the Pt/CeO_(2)/GO biosensor containing 0.47 at% platinum exhibits an excellent electrochemical detection performance with high sensitivity of 12.3 μA·L/(mmol·cm^(2)) and a low limit of detection(LOD) of 5.12 μmol/L in a wide linear range from 10 to 900 μmol/L.In addition,the proposed biosensor possesses a promising anti-interference capability,as well as high stability and good reproducibility,which was assessed by testing the cyclic voltammogram in 0.1 mol/L lactic acid one year later.The underlying mechanism was proposed for electrochemical oxidation of LA to carbon dioxide and acetic acid with the synergistic effect among Pt,CeO_(2),and GO.Furthermore,the results of the standard addition method in real samples(human serum and urine samples) reveal that the lactic acid detection of the non-enzymatic Pt/CeO_(2)/GO biosensor is accompanied by high reliability.Thus,it will be a valuable biosensor for in vitro detection of lactic acid level in clinical samples.

Evaluation of In Vitro Enzymatic and Non-Enzymatic Antioxidant Properites of Leaf Extract from Alpinia Purpurata(Vieill.) K. Schum.

Objective： To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. Methods： One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4 ℃ for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase（SOD）, catalase（CAT）, glutathione peroxidase（GPx）, glutathione S-transferase（GST）, ascorbate oxidase, peroxidase, polyphenol oxidase（PPO） and non-enzymatic antioxidants such as vitamin C, total reduced glutathione（TRG） and lipid peroxidation（LPO）. Results： The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein, GST 372.11±5.70 μmol of 1-chloro 2,4 dinitrobenzene（CDNB）-reduced glutathione（GSH） conjugate formed/min/mg protein, GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 μmol/g tissue, TRG 75.27±3.55 μg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 μmol of H_2O_2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 μmol/g tissue in moderate amount. Conclusion： Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent.

熟地黄多糖对断奶仔猪抗氧化性能和免疫性能的影响

为探讨熟地黄多糖对断奶仔猪免疫性能和抗氧化性能的影响,选取体重相近的杜洛克×长白×大白25日龄断奶仔猪60头,随机分成对照和4个试验组,每组12头仔猪,分别喂给添加熟地黄多糖0、0.04%、0.08%、0.12%和0.16%的日粮。结果表明:各试验组血清丙二醛含量显著低于对照组,血清谷胱甘肽过氧化物酶和超氧化物歧化酶活性均显著高于对照组,0.04%和0.16%熟地黄多糖组过氧化氢酶活性显著高于其他组,总抗氧化能力是随着熟地黄多糖添加量增加而逐渐升高。各组的血清免疫球蛋白IgA、IgM和IgG含量差异不显著;0.04%熟地黄多糖组血清乳酸含量降低最显著(34.9%);0.12%熟地黄多糖组的血清白蛋白和总蛋白含量最高(39.04g/L、59.82 g/L);各试验组的血清乳酸脱氢酶活性均低于对照组,0.16%熟地黄多糖显著提高血清溶菌酶活性(提高144.7%),而其他3个试验组对血清溶菌酶活性影响不显著。综上所述,熟地黄多糖能够显著提高断奶仔猪抗氧化性能以及血清白蛋白和总蛋白水平,以0.12%的添加量效果最好。

不同浓度Cd对小白菜生长及抗氧化系统的影响

采用温室水培试验方法,研究了不同浓度Cd对小白菜生长、叶绿素含量和叶片中抗氧化系统的影响。结果表明,Cd浓度为0.01mg·L-1时促进小白菜生长;Cd浓度为10mg·L-1时,抑制小白菜生长;与对照相比,0.01、0.1mg·L-1Cd处理后,叶绿素a与叶绿素b的含量增加;Cd浓度达到1mg·L-1时,叶绿素a与叶绿素b的含量显著下降(P<0.05)。超氧化物岐化酶(SOD)的活性随Cd处理浓度的增加而下降;愈创木酚过氧化物酶(GPOD)、抗坏血酸过氧化物酶(APX)在Cd浓度为0.1mg·L-1时活性最高;过氧化氢酶(CAT)在Cd浓度为1mg·L-1时活性达到最高;脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)的活性和抗坏血酸(AsA)、还原型谷胱甘肽(GSH)的含量在Cd处理浓度为10mg·L-1时最高。

Mn对超富集植物短毛蓼(Polygonum pubescens Blume)抗氧化机理的影响

采用水培的方法,研究了不同浓度Mn(0、0.5、1、2、4、8mmol·L-1)对新发现的Mn超富集植物短毛蓼(Polygonum pubescens Blume)生长、Mn吸收及Mn对其抗氧化酶和非酶系统的影响。结果表明,锰处理显著增加了(P<0.05)短毛蓼根、茎、叶中Mn的含量,锰处理还引起了短毛蓼叶片中过氧化氢(H2O2)和丙二醛(MDA)的累积。当Mn处理浓度大于1mmol·L-1时,显著降低了短毛蓼的株高、株重(P<0.05);当Mn处理浓度为8mmol·L-1时,短毛蓼叶片中叶绿素a、叶绿素b、叶绿素a+b含量最低,与对照差异显著(P<0.05)。Mn处理显著提高了短毛蓼叶片中超氧物歧化酶(SOD)活性(P<0.05),而过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性呈先升后降趋势,表明Mn处理打破了短毛蓼活性氧物质的正常代谢,并启动了抗氧化酶系统。8mmol·L-1的Mn处理,显著提高了短毛蓼叶片中巯基(-SH)、还原型谷胱甘肽(GSH)的含量(P<0.05),比对照分别提高了10.6%和20%,表明-SH、GSH在短毛蓼缓解Mn毒害的过程中起着重要作用。

外源ABA对低温胁迫下蝴蝶兰叶片生理指标的影响

为研究脱落酸(ABA)对蝴蝶兰抗寒性的影响,以蝴蝶兰中苗为试材,叶面喷施50 mg.L-1脱落酸(ABA)1 d后,于6℃低温胁迫4 d,以清水喷施为对照,在低温处理0,1,2,3和4 d后测定叶片相对电导率、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性。结果表明:喷施ABA可以延缓蝴蝶兰叶片的相对电导率和MDA含量的上升,减少Pro的累积量,提高CAT和SOD的活性。说明喷施ABA提高了蝴蝶兰叶片的抗氧化酶活性,减少了膜脂过氧化产物的积累,降低了细胞膜透性,从而减轻了低温胁迫的伤害。

大蒜多糖抗氧化活性及其对PC12细胞增殖的影响

目的:检测大蒜多糖对大鼠嗜铬瘤细胞株(pheochromocytom a cells,PC12)增殖的影响和对经过氧化氢(H2O2)诱导损伤的PC12细胞的保护作用。方法:应用四氮唑蓝(MTT)比色法检测大蒜多糖对细胞增殖的影响;建立H2O2致PC12细胞损伤模型,于倒置相差显微镜下观察细胞形态的变化;化学比色法测定乳酸脱氢酶(LDH)释放量及细胞内和细胞培养上清液中丙二醛(MDA)含量和超氧化物歧化酶(SOD)的活性。结果:大蒜多糖各剂量组均能显著提高正常PC12细胞的存活数,大蒜多糖各剂量组均能有效对抗由25μmol/L H2O2引起的细胞存活率下降和细胞凋亡,可明显改善细胞形态的衰变,显著降低LDH释放量和细胞培养液及细胞内的MDA含量,提高SOD活性。结论:大蒜多糖促进正常PC12细胞增殖;且对H2O2诱导PC12细胞损伤具有保护作用,其作用机制可能与提高PC12细胞的抗氧化能力有关。

丹参防治顺铂肝毒性的实验研究

顺铂广泛用于治疗各种实体瘤,但由于其在肝脏蓄积,且肝脏对顺铂敏感性高[1],高剂量给药或低剂量重复给药,患者肝毒性频发。氧化应激与顺铂所致肝脏病理损伤有密切关联[2]。而丹参具有抗氧化,清除脂质过氧化物的功效[3]。因此,本课题旨在研究丹参对顺铂给药小鼠肝功能的影响,并探讨其作用机制。