Pregnancy Outcomes for Day 5 Versus Day 6 Single Frozen-thawed Blastocyst Transfer with Different Qualities of Embryos: A Large Matched-cohort Study

Objective This study aimed to determine whether the day of blastocyst expansion affects pregnancy outcomes in frozen-thawed blastocyst transfer(FBT)cycles.Methods A retrospective match-cohort study was conducted.Patients who underwent blastocyst transfer in frozen-thawed cycles at day 5 or 6 were matched for potential confounding factors.A total of 2207 matched pairs of FBT cycles were included from January 2016 to December 2019 in our Reproductive Medicine Center.Results The clinical pregnancy rate(CPR)and live birth rate(LBR)were significantly increased in day 5 blastocyst transfers when compared to day 6 blastocyst transfers,in terms of the same embryo quality.For FBT cycles with good-quality embryo,the CPR at day 5 and 6 was 61.30%and 57.56%,respectively(P=0.045),and the LBR was 44.79%and 36.16%,respectively(P<0.001).For FBT cycles with poor-quality embryo,the CPR at day 5 and 6 was 48.61%and 40.89%,respectively(P=0.006),and the LBR was 31.71%and 25.74%,respectively(P=0.019).The CPR for FBT cycles with good-quality embryo was statistically higher at day 6 than that at day 5 with poor-quality embryo transferred(57.56%vs.48.61%,P=0.001).Maternal age,anti-Müllerian hormone(AMH),endometrial thickness,embryo quality,and the day of blastocyst expansion were independently correlated with the CPR and LBR.The FBT cycles at day 5 had significantly higher CPR(adjusted odds ratio[OR]=1.246,95%confidence intervals[CI]:1.097–1.415,P=0.001)and LBR(adjusted OR=1.435,95%CI:1.258–1.637,P<0.001)than those at day 6.Conclusion The embryo quality is the primary indicator for FBT cycles.Day 5 blastocysts should be preferred when the quality of embryo at day 5 is the same as that at day 6.

Cultivate Innovative and Entrepreneurial Qualities among Medical Students through Curriculum Ideology and Politics

As the main body of promoting the development of medical science and technology, medical students play an extremely important role in promoting the construction of new medical science. New medicine is a new concept based on the development of traditional medicine, breaking the discipline barrier, aiming to cultivate composite talents, taking reality as the orientation, and combining with the Internet, big data and artificial intelligence. Cultivating medical students’ innovation and entrepreneurship literacy is not only the internal need of the construction of innovative medical talents in new medical sciences, but also the development need of promoting the optimization of medical disciplines and effectively promoting the construction of an innovative country. As the main place for training medical talents, medical colleges and universities should integrate classroom ideology and politics with the new medical background to improve medical students’ professional knowledge, professional ethics, humanistic quality and scientific and technological quality, promote the cultivation of medical students’ innovative spirit, and further innovate medical theories, technologies and concepts. It lays a solid foundation for cultivating top medical talents with both innovative thinking and cultural self-confidence and building an innovative country.

QTL Mapping and Q×E Interactions of Grain Cooking and Nutrient Qualities in Rice Under Upland and Lowland Environments

Grain cooking and nutrient qualities are the most important components of rice （Oryza sativa L.） quality. A doubled haploid （DH） population from a cross between two japonica cultivars was used to examine the phenotypic values and potential QTLs for the quality traits. The cooking and nutrient quality traits, including the amylose content （AC）, the gel consistency （CJC）, the gelatinization temperature （GT）, and the protein content （PC）, in rice grown under upland and lowland environments were evaluated. Significant differences for AC, GC, GT, and PC between upland and lowland environments were detected. The phenotypic values of all four traits were higher under upland environment than lowland environment. The value of PC under upland environment was significantly higher （by 37.9%） than that under lowland environment. This suggests that upland cultivation had large effect on both cooking and nutrient qualifies. A total of seven QTLs and twelve pairs of QTLs were detected to have significant additive and epistatic effects for the four traits. Significant Q x E interaction effects of two QTLs and two pairs of QTLs were also discovered. The general contribution of additive QTLs ranged from 1.91% to 19.77%. The Q × E interactions of QTLs QGt3 and QAc6 accounted for 8.99% and 47.86% of the phenotypic variation, respectively, whereas those of the 2 pairs of epistatic QTLs, QAc6-QAcllb and QAc8-QAc9, accounted for 32.54% and 11.82%, respectively. Five QTLs QGt6b, QGt8, QGt11, QGcl, and QPc2, which had relatively high general contribution and no Q x E interactions, were selected to facilitate the upland rice grain quality breeding.

Effects of Different Light Qualities on Activity and Gene Expression of Caspase-like Proteases in Tobacco Leaves

[Objective] The study aimed to investigate the activity and gene expression of caspase-like proteases in tobacco leaves growing under different light qualities. [Method] By covering tobacco plants with white, red, yellow, blue and purple films to obtain different light quality, the changes of chlorophyll content, activity and gene expression of caspase-like proteases in the tobacco leaves were studied. [Results] Compared with treatments of white, red and yellow film, blue and purple films delayed the decrease of chlorophyll content and senescence of tobacco leaves at the late growth stage, and relatively lowered the activity and gene expression of caspase-like proteases during growth, development and senescence periods. [Conclusion] Different light qualities exhibited various effects on the growth, development and senescence of tobacco leaves, possibly by affecting the activity and gene expression of caspase-like proteases to some extent.

Study on Influences of Different Land Use Types on Soil Qualities in Plateau Karst Depression

[Objective] The aim was to study influences of different land use ways on soil qualities in plateau karst depression.[Method] Different land use types were divided by topographic map of 1∶10 000 scales and soil properties of physics and chemistry were analyzed.[Result] The results showed that the variation trend of soil depth(A+B stratum)was slope plough(include returning farm to forest)land>natural forest land>planted forest land>shrub forest land,and relative indexes changing regularities of soil...

Comparative Analysis on Fruit Qualities of 7 Litchi Varieties from Vietnam

[Objective] The aim was to screen litchi varieties suitable for local production in Vietnam. [Method] The fruit botanical and quality characteristics of the seven litchi varieties introduced into Northern Mountainous Agriculture and Forestry Science Institute of Vietnam Academy of Agriculture Sciences were compared and analyzed. [Result] Three varieties with good general qualities including Lai Phu Ho,Thach Binh and Hung Long which were suitable to be cultivated in the local area were selected. In addition,Phu Dong was the available,early-maturing germplasm resource. [Conclusion] This research will provide reference for litchi production and breeding in northern mountainous area.

Comparative Analysis on Fruit Qualities of Longan Varieties form Vietnam

[Objective] The aim was to screen longan varieties suitable for local production in Vietnam.[Method] The fruit botanical characteristics and quality characteristics of longan varieties introduced into Northern Mountainous Agriculture and Forestry Science Institute of Vietnam Academy of Agriculture Sciences were compared and analyzed.[Result] Two varieties with good general qualities including PMP6 and PHS1-1 which were suitable to be cultivated in the local area were selected.In addition,Phu Dong was the available processed germplasm resource.[Conclusion] This research will provide reference for longan production and breeding in Northern Mountainous.

Fundamental Qualities of Diplomatic Interpreters

With the more and more frequent of international communications, diplomatic work has come to a totally new age.The diplomatic interpreters must meet higher requirments and challenges. Hence what qualities they should have becomes an important problem.this thesis focus on four aspects to deal with it.

Investigation of Punica granatum L.Germplasm Resources in Binchuan and Comparative Analysis of Major Fruit Qualities

[Objective] This study aimed to provide a basis for enriching the Punica granatum L. germplasm resources through investigating Punica granatum L. vari- eties in Binchuan. [Method] In this study, biological traits of Punica granatum L. vari- eties in Binchuan （including two local varieties-＇Binchuan Red Coat＇ and ＇Binchuan Green Coat＇, ＇Cyan Coat and Soft Seeds＇ and ＇Little Red Coat＇ introduced from Huili, Sichuan, ＇Acid Pomegranate＇ introduced from Shandong Province and ＂Ma Pomegranate＂ from Fengqiu, Henan） were investigated and their major fruit qualities were also analyzed. [Result] ＂Cyan Coat and Soft Seeds＇ and ＂Ma Pomegranate＇ grew well with good fruit quality, which adapted well to the climate and environment in Binchuan; ＂Little Red Coat＇ showed average performance in growth and major fruit qualities with inferior flavour; ＂Binchuan Red Coat＇ and ＇Binchuan Green Coat＇ also showed average performance in major fruit qualities and and inferior flavour; ＇Acid Pomegranate＇ grew while was inferior in flavour. [Conclusion] This study pro- vides references for presenting the directions for studies of P. granatum L. germplasm resources and determining the genetic relationship among germplasm resources, as well as breeding, introduction and cultivation of P. granatum L., further providing a variety basis for pomegranate production.

Changes in Cooking and Nutrition Qualities of Grains at Different Positions in a Rice Panicle under Different Nitrogen Levels

Cooking and nutrition qualities of grain are two of the most important aspects of rice quality. To understand the difference in cooking and nutrition qualities among the grains at different positions within a rice panicle, the distributions of gel consistency （GC）, amylose content （AC） and crude protein content （CPC） of grains in a panicle in association with different nitrogen levels （0, 120 and 240 k c/ha） were investigated by using two rice varieties, Yangdao 6 （indica） and Wuyujing 3 （japonica）. In general, the grains at the basal part of a panicle had lower GC and higher AC than those at the upper or middle part of a panicle. The 1^st grain on the secondary branch with earlier flowering exhibited the highest GC, whereas the 2nd grain on the primary branch with later flowering showed the highest AC. For Yangdao 6, CPC in the grains on the primary branches was lower at the middle part of a panicle than at the upper or basal part of a panicle. For Wuyujing 3, there were no significant differences in CPCs in the grains among the upper, middle and basal parts of a panicle. GC in the grains was increased, whereas AC was reduced from zero nitrogen application （ON） to low amount of nitrogen application （LN）, and the result was reversed from LN to medium amount of nitrogen application （MN）. CPC was increased with the increase in the amount of nitrogen application.

Effects of Chalkiness on Cooking,Eating and Nutritional Qualities of Rice in Two indica Varieties

An experiment was conducted to investigate the effects of chalkiness on cooking, eating and nutrition qualities of rice using Gangyou 527 （indica hybrid rice） and Zhaiyeqing 8 （conventional indica rice） as materials. Compared with the milled rice without chalkiness, amylose content, final viscosity, setback and consistence increased significantly, while gel consistence, peak viscosity and breakdown decreased remarkably and other RVA values did not have significant changes in the chalky rice of Gangyou 527. The differences in the above indices between the chalky and non-chalky rice was not remarkable in Zhaiyeqing 8. The crude protein content of chalky rice in Zhaiyeqing 8 was significantly lower than that of non-chalky rice, but there was no remarkable change between the chalky and non-chalky rice in Gangyou 527. Glutelin content fell significantly, and albumin, globulin, prolamine and lysine contents did not change significantly in chalky rice compared with non-chalky rice in the two varieties.

Principal Component Analysis of Cooked Rice Texture Qualities

Texture qualities of cooked rice are comprised of many indexes with the complex relationship, so it is difficult to analyze and evaluate cooked rice. In this paper, the related indexes of texture properties were conversed into the independent indexes of principal component based on the principal component analysis method. The results showed that the rice kernel types influenced the meanings of principal components indexes. For long and short rice, the first principal component was comprehensive index. But the second principal component was springiness for the short rice, while it was adhesiveness for long rice. Therefore, the first principal component can be used to express the quality of cooked rice with a few of indexes, and the rice type can be recognized according to the second principal component.

Correlation Between Physicochemical Properties and Eating Qualities of Rice

Correlation among physicochemical properties, which include amylose content, alkali spreading values, gel consistency, water absorption, expansion rate, solid content of rice-water, protein content and fat content, and eating qualities of six kinds of rice samples planted in Heilongjiang Province were studied. Correlation analysis showed that amylose content, water absorption and expansion rate were negatively correlated with eating qualities, yet gel consistency, alkali spreading values, solid content of rice-water and fat content were positively correlated with eating qualities. Among them, eating quality had an obvious correlation with amylose content and gel consistency, but no significant correlation with protein content. The regression equation, which described the relationship of the eating quality scores and physicochemical indexes, was Y=94.439–12.711X1–23.721X2–0.701X3＋0.570X4＋186.938X5（X1, X2, X3, X4 and X5 represented amylose content, water absorption, expansion rate gel, consistency and fat content）. The single factor analysis of variance showed that six kinds of rice existed significant differences in quality category.

Applying multivariate statistics for identification of groundwater chemistry and qualities in the Sugan Lake Basin, Northern Qinghai-Tibet Plateau, China

The Sugan Lake Basin is located in the inland arid region of northwestern China,in which groundwater is of great significance to human and ecology.Therefore,it is necessary to understand the chemical characteristics and quality of groundwater in the basin.Based on samples collected from 35 groundwater wells in Sugan Lake Basin,the spatial distribution characteristics of groundwater chemistry,main hydrogeochemical processes and groundwater quality have been discussed in this paper by using the multivariate statistics and hydrochemistry analysis methods.The results showed that the groundwater is weakly alkaline,and its total dissolved solid(TDS)and total hardness(TH) are high,with the average values of 1244.03 mg/L and 492.10 mg/L,respectively.The types of groundwater are mainly HCO_3^--SO_4^(2-)-Ca^(2+)type in the runoff area and Cl^--SO_4^(2-)-Na^+type in the catchment area.Rock weathering and ion exchange are the main controlling factors of regional groundwater chemistry,followed by evaporative crystallization,and human activities have less impact on groundwater.The spatial difference of groundwater quality is obvious,the water quality of the catchment area is not suitable for drinking,and the suitability for plant growth is also poor.The groundwater in the runoff area can be used for drinking,but the hardness is slightly higher,which is more suitable for ecological purpose.

Relationship between malt qualities and β-amylase activity and protein content as affected by timing of nitrogen fertilizer application

The effects of different timing of N fertilizer application at the same rate on grain β-amylase activity, protein con- centration, weight and malt quality of barley were studied. Grain β-amylase activity and protein concentration were significantly higher in treatments where all top-dressed N fertilizer was applied at booting stage only or equally applied at two-leaf stage and booting stage than in the treatment where all top-dressed N fertilizer was applied at two-leaf age stage only. On the other hand, grain weight and malt extract decreased with increased N application at booting stage. There were obvious differences between barley varieties and experimental years in the grain and malt quality response to the timing of N fertilizer application. It was found that grain protein concentration was significantly and positively correlated with β-amylase activity, but significantly and nega- tively correlated with malt extract and Kolbach index. The effect of grain protein concentration on malt quality was predominant over the effect of grain β-amylase activity.

Variation of Grain and Malt Qualities in Barley as Affected by Cultivars and Environments

Inferior and unstable quality of malt barley in China has limited its wide use in malting and brewing industries. In this research, eight two-rowed barley cultivars were planted at 7 locations with different ecological conditions in southern China, to investigate the cultivar and environmental effect on grain and malt qualities. The results showed that grain protein content differed dramatically among locations, but there were no significant differences among cultivars. For four malt qualities including diastatic power, wort viscosity, Kolbach index and malt extract, significant differences were found among cultivars and locations, except for diastatic power among cultivars. Coefficients of variance(CV)caused by location were greater than those caused by cultivar for each quality parameter, especially for diastatic power and Kolbach index, indicating the predominant influence of environment on malt quality. The analysis showed that grain protein content was positively related to diastatic power and wort viscosity, and negatively to malt extract. Kolbach index was positively related to malt extract, and negatively to diastatic power and wort viscosity. Diastatic power showed positive and negative correlation with wort viscosity and malt extract, respectively.

Differential expressions among five Waxy alleles and their effects on the eating and cooking qualities in specialty rice cultivars

Eating and cooking qualities（ECQs） of rice are important attributes due to its major influence on consumer acceptability. To better understand the molecular mechanism of the variation in ECQs, we investigated and compared the expressions among different alleles of the Waxy（Wx） gene and its effect on ECQs in specialty rice cultivars. The results showed that the accumulation of amylose was positively and significantly correlated to the level of mature Wx m RNA and granule-bound starch synthase I（GBSS I） in developing rice grain at 12 days after flowering. The amount of GBSS I and its activity together are the main factors controlling amylose synthesis. Differences in ECQs among five Wx allele types were investigated in samples from 15 rice varieties. The apparent amylose content（AAC） and gel consistency（GC） were similar in each type of Wx allele. The AAC followed the order, Wx^a type〉Wx^in type〉Wx^b type〉Wx^mq type〉wx. Contrary to this, the GC showed an opposite trend compared to AAC. There was a wide variation in rapid visco analyzer（RVA） profile among five Wx allele types, while varieties sharing a specified Wx allele had basically the similar RVA profile, although there was a slight difference in some RVA parameters, peak, hot paste and cool paste viscosities.

Genome Editing as A Versatile Tool to Improve Horticultural Crop Qualities

The quality traits of horticultural crops,including the accumulation of nutrients and flavor substances,morphology,and texture,affect the palatability and nutritional value.For many years,efforts have been made to improve the quality of horticultural crops.The recent establishment of gene editing technology,with its potential applications in horticultural crops,provides a strategy for achieving this goal in a rapid and efficient manner.Here,we summarize research efforts aimed at improving horticultural crop quality through genome editing.We describe specific genome editing systems that have been used and traits that have been targeted in these efforts.Additionally,we discuss limiting factors and future perspectives of genome editing technology in improving horticultural crop qualities in both research and plant breeding.In summary,genome editing technology is emerging as a powerful tool for efficiently and rapidly improving horticultural crop quality,and we believe that the cautious application of genome editing in horticultural crops will generate new germplasms with improved quality in the near future.

Withdrawal time in excellent or very poor bowel preparation qualities

AIM: To evaluate association(s) between withdrawal time and polyp detection in various bowel preparation qualities. METHODS: Retrospective cohort analysis of screening colonoscopies performed between January 2005 and June 2011 for patients with average risk of colorectal cancer. Exclusion criteria included patients with a personal history of adenomatous polyps or colon cancer, prior colonic resection, significant family history of colorectal cancer, screening colonoscopy after other abnormal screening tests such as flexible sigmoidoscopy or barium enema, and screening colonoscopies during in-patient care. All procedures were performed or directly supervised by gastroenterologists. Main measurements were number of colonic segments with polyps and total number of colonic polyps.RESULTS: Multivariate analysis of 8331 colonosco-pies showed longer withdrawal time was associated with more colonic segments with polyps in good(adjusted OR = 1.16; 95%CI: 1.13-1.19), fair(OR = 1.13; 95%CI: 1.10-1.17), and poor(OR = 1.18; 95%CI: 1.11-1.26) bowel preparation qualities. A higher number of total polyps was associated with longer withdrawal time in good(OR = 1.15; 95%CI: 1.13-1.18), fair(OR = 1.13; 95%CI: 1.10-1.16), and poor(OR = 1.20; 95%CI: 1.13-1.29) bowel preparation qualities. Longer withdrawal time was not associated with more colonic segments with polyps or greater number of colonic polyps in bowel preparations with excellent(OR = 1.07, 95%CI: 0.99-1.26; OR = 1.11, 95%CI: 0.99-1.24, respectively) and very poor(OR = 1.02, 95%CI: 0.99-1.12; OR = 1.05, 95%CI: 0.99-1.10, respectively) qualities.CONCLUSION: Longer withdrawal time is not associated with higher polyp number detected in colonoscopies with excellent or very poor bowel preparation quality.

Comparing Studies on the General Egg Qualities and Mineral Element Contents of Different Layers

[Objective]In order to compare the general egg qualities and mineral element contents of different layers,six commercial breeds in brown shell layer,including Lohmann,Jinghong,Hyline,Xinyang,Hisax,ISA,Suqing green shell layer and Wenchang chicken were used as materials.The general egg quality traits and mineral elements contents of eight breeds at 43 weeks old were mensurated.[Result] There were significant differences in general egg qualities（except yolk weight）of eight breeds.About the mineral element contents,the differences were significant only in P,Mn and Se of some breeds in this experiment.[Conclusion]The results in this study could provide the reference for further researcher,layer producers,nutritionists,consumers and so on.