Objective： To explore the effects of Livin gene knock down using sequence-specific siRNA on apoptosis of human breast cancer cell ZR-75-30. Methods： Chemically synthesized double stranded RNA（dsRNA） targeting Livi...Objective： To explore the effects of Livin gene knock down using sequence-specific siRNA on apoptosis of human breast cancer cell ZR-75-30. Methods： Chemically synthesized double stranded RNA（dsRNA） targeting Livin was transfected into human breast cancer cell ZR-75-30 with lipofectamineTM2000. The transfection efficiency was observed under a fluorescence confocal microscope. Expression of Livin at both mRNA and protein levels were detected by reverse transcription-polymerase chain reaction（RT-PCR） and immunohistochemical analysis. The effects on apoptosis of ZR-75-30 cells were assessed by FCAS. Results： The Livin siRNA can effectively and specifically inhibited the expression of Livin gene in ZR-75-30. The inhibition rate was 53.66% at mRNA level and 58.32% at protein level. After 24h, （8.36_.＋0.20）%cells transfected with siRNA were induced to apoptosis. Conclusion： Chemically synthesized short Livin-siRNA can effectively inhibit Livin over expression and remarkably induce apoptosis in human breast cancer cell line ZR-75-30. Livin RNAi has a potential value in gene therapy of breast cancer.展开更多
基金the Education Committee Foundation of Liaoning Province(No.05L506)
文摘Objective： To explore the effects of Livin gene knock down using sequence-specific siRNA on apoptosis of human breast cancer cell ZR-75-30. Methods： Chemically synthesized double stranded RNA（dsRNA） targeting Livin was transfected into human breast cancer cell ZR-75-30 with lipofectamineTM2000. The transfection efficiency was observed under a fluorescence confocal microscope. Expression of Livin at both mRNA and protein levels were detected by reverse transcription-polymerase chain reaction（RT-PCR） and immunohistochemical analysis. The effects on apoptosis of ZR-75-30 cells were assessed by FCAS. Results： The Livin siRNA can effectively and specifically inhibited the expression of Livin gene in ZR-75-30. The inhibition rate was 53.66% at mRNA level and 58.32% at protein level. After 24h, （8.36_.＋0.20）%cells transfected with siRNA were induced to apoptosis. Conclusion： Chemically synthesized short Livin-siRNA can effectively inhibit Livin over expression and remarkably induce apoptosis in human breast cancer cell line ZR-75-30. Livin RNAi has a potential value in gene therapy of breast cancer.
