Surface Modification of Biomimetic PLGA-(ASP-PEG) Matrix with RGD-Containing Peptide:a New Non-Viral Vector for Gene Transfer and Tissue Engineering

RGD-containing peptide (K16-GRGDSPC), characterized as non-viral gene vectors, was fabricated to modify the surface of PLGA-ASP-PEG matrix, which offered the foundation for gene transfer with porous matrix of gene activated later. Peptide was synthesized and matrix was executed into chips A,B and chip C. Chip C was regarded as control. Chips A and B were reacted with cross-linker. Then chip A was reacted with peptide. MS and HPLC were used to detect the MW and purity of peptide. Sulphur, existing on the surface of biomaterials, was detected by XPS. The purity of un-reacted peptide in residual solution was detected by a spectrophotometer. HPLC shows that the peptide purity was 94%-95%, and MS shows that the MW was 2 741.3307. XPS reveals that the binding energy of sulphur was 164 eV and the ratio of carbon to sulphur (C/S) was 99.746 ∶0.1014 in reacted chip A. The binding energy of sulphur in reacted chip B was 164 eV and 162 eV, C/S was 99.574∶0.4255, and there was no sulphur in chip C. Peptide was manufactured and linked to the surface of biomimetic and 3-D matrix, which offered the possibilities for gene transfer and tissue engineering with this new kind of non-viral gene vector.

In vitro effect of p2l^(WAF-1/CIP1) gene on growth of human glioma cells mediated by EGFR targeted non-viral vector GE7 system

To construct the EGFR targeted non-viral vector GE7 system and explore the in vitro effect of p21^WAF-1/CIP1 gene on growth of human glioma cells mediated by the GE7 system. Methods: The EGFR targeted non-vi-ral vector GET gene delivery system was constructed. The malignant human glioma cell llne U251MG was transfected in vitro with β-galactosidase gene( reporter gene) and p21^WAF-1/CIP1 gene (therapeutic gene) using the GET system. By means of X-gal staining, MTS and FACS, the transfection efficiency of exogenous gene and apoptosis rate of tumor cells were examined. The expression of p21^WAF-1/CIP1 gene in transfected U251MG cell was examined by immunohistochemitry staining. Results: The highest transfer rate of exogenous gene was 70%. After transfection with p21^WAF-l/CIP1 gene,the expression of WAF-1 increased remarkably and steadily; the growth of U251MG cells were inhibited evidently.FACS examination showed G1 arrest. The average apoptosis rate was 25.2%. Conclusion: GE7 system has the ability to transfer exogenous gene to targeted cells efficiently, and expression of p21^WAF-l/CIP1 gene can induce apoptosis of glio-ma cell and inhibit its growth.

AHEVCVideo Steganalysis Method Using the Optimality of Motion Vector Prediction

Among steganalysis techniques,detection against MV(motion vector)domain-based video steganography in the HEVC(High Efficiency Video Coding)standard remains a challenging issue.For the purpose of improving the detection performance,this paper proposes a steganalysis method that can perfectly detectMV-based steganography in HEVC.Firstly,we define the local optimality of MVP(Motion Vector Prediction)based on the technology of AMVP(Advanced Motion Vector Prediction).Secondly,we analyze that in HEVC video,message embedding either usingMVP index orMVD(Motion Vector Difference)may destroy the above optimality of MVP.And then,we define the optimal rate of MVP as a steganalysis feature.Finally,we conduct steganalysis detection experiments on two general datasets for three popular steganographymethods and compare the performance with four state-ofthe-art steganalysis methods.The experimental results demonstrate the effectiveness of the proposed feature set.Furthermore,our method stands out for its practical applicability,requiring no model training and exhibiting low computational complexity,making it a viable solution for real-world scenarios.

A semantic vector map-based approach for aircraft positioning in GNSS/GPS denied large-scale environment

Accurate positioning is one of the essential requirements for numerous applications of remote sensing data,especially in the event of a noisy or unreliable satellite signal.Toward this end,we present a novel framework for aircraft geo-localization in a large range that only requires a downward-facing monocular camera,an altimeter,a compass,and an open-source Vector Map(VMAP).The algorithm combines the matching and particle filter methods.Shape vector and correlation between two building contour vectors are defined,and a coarse-to-fine building vector matching(CFBVM)method is proposed in the matching stage,for which the original matching results are described by the Gaussian mixture model(GMM).Subsequently,an improved resampling strategy is designed to reduce computing expenses with a huge number of initial particles,and a credibility indicator is designed to avoid location mistakes in the particle filter stage.An experimental evaluation of the approach based on flight data is provided.On a flight at a height of 0.2 km over a flight distance of 2 km,the aircraft is geo-localized in a reference map of 11,025 km~2using 0.09 km~2aerial images without any prior information.The absolute localization error is less than 10 m.

An Initial Perturbation Method for the Multiscale Singular Vector in Global Ensemble Prediction

Ensemble prediction is widely used to represent the uncertainty of single deterministic Numerical Weather Prediction(NWP) caused by errors in initial conditions(ICs). The traditional Singular Vector(SV) initial perturbation method tends only to capture synoptic scale initial uncertainty rather than mesoscale uncertainty in global ensemble prediction. To address this issue, a multiscale SV initial perturbation method based on the China Meteorological Administration Global Ensemble Prediction System(CMA-GEPS) is proposed to quantify multiscale initial uncertainty. The multiscale SV initial perturbation approach entails calculating multiscale SVs at different resolutions with multiple linearized physical processes to capture fast-growing perturbations from mesoscale to synoptic scale in target areas and combining these SVs by using a Gaussian sampling method with amplitude coefficients to generate initial perturbations. Following that, the energy norm,energy spectrum, and structure of multiscale SVs and their impact on GEPS are analyzed based on a batch experiment in different seasons. The results show that the multiscale SV initial perturbations can possess more energy and capture more mesoscale uncertainties than the traditional single-SV method. Meanwhile, multiscale SV initial perturbations can reflect the strongest dynamical instability in target areas. Their performances in global ensemble prediction when compared to single-scale SVs are shown to(i) improve the relationship between the ensemble spread and the root-mean-square error and(ii) provide a better probability forecast skill for atmospheric circulation during the late forecast period and for short-to medium-range precipitation. This study provides scientific evidence and application foundations for the design and development of a multiscale SV initial perturbation method for the GEPS.

Improved Twin Support Vector Machine Algorithm and Applications in Classification Problems

The distribution of data has a significant impact on the results of classification.When the distribution of one class is insignificant compared to the distribution of another class,data imbalance occurs.This will result in rising outlier values and noise.Therefore,the speed and performance of classification could be greatly affected.Given the above problems,this paper starts with the motivation and mathematical representing of classification,puts forward a new classification method based on the relationship between different classification formulations.Combined with the vector characteristics of the actual problem and the choice of matrix characteristics,we firstly analyze the orderly regression to introduce slack variables to solve the constraint problem of the lone point.Then we introduce the fuzzy factors to solve the problem of the gap between the isolated points on the basis of the support vector machine.We introduce the cost control to solve the problem of sample skew.Finally,based on the bi-boundary support vector machine,a twostep weight setting twin classifier is constructed.This can help to identify multitasks with feature-selected patterns without the need for additional optimizers,which solves the problem of large-scale classification that can’t deal effectively with the very low category distribution gap.

Differentially Private Support Vector Machines with Knowledge Aggregation

With the widespread data collection and processing,privacy-preserving machine learning has become increasingly important in addressing privacy risks related to individuals.Support vector machine(SVM)is one of the most elementary learning models of machine learning.Privacy issues surrounding SVM classifier training have attracted increasing attention.In this paper,we investigate Differential Privacy-compliant Federated Machine Learning with Dimensionality Reduction,called FedDPDR-DPML,which greatly improves data utility while providing strong privacy guarantees.Considering in distributed learning scenarios,multiple participants usually hold unbalanced or small amounts of data.Therefore,FedDPDR-DPML enables multiple participants to collaboratively learn a global model based on weighted model averaging and knowledge aggregation and then the server distributes the global model to each participant to improve local data utility.Aiming at high-dimensional data,we adopt differential privacy in both the principal component analysis(PCA)-based dimensionality reduction phase and SVM classifiers training phase,which improves model accuracy while achieving strict differential privacy protection.Besides,we train Differential privacy(DP)-compliant SVM classifiers by adding noise to the objective function itself,thus leading to better data utility.Extensive experiments on three high-dimensional datasets demonstrate that FedDPDR-DPML can achieve high accuracy while ensuring strong privacy protection.

Diffraction deep neural network-based classification for vector vortex beams

The vector vortex beam(VVB)has attracted significant attention due to its intrinsic diversity of information and has found great applications in both classical and quantum communications.However,a VVB is unavoidably affected by atmospheric turbulence(AT)when it propagates through the free-space optical communication environment,which results in detection errors at the receiver.In this paper,we propose a VVB classification scheme to detect VVBs with continuously changing polarization states under AT,where a diffractive deep neural network(DDNN)is designed and trained to classify the intensity distribution of the input distorted VVBs,and the horizontal direction of polarization of the input distorted beam is adopted as the feature for the classification through the DDNN.The numerical simulations and experimental results demonstrate that the proposed scheme has high accuracy in classification tasks.The energy distribution percentage remains above 95%from weak to medium AT,and the classification accuracy can remain above 95%for various strengths of turbulence.It has a faster convergence and better accuracy than that based on a convolutional neural network.

Performance Analysis of Support Vector Machine (SVM) on Challenging Datasets for Forest Fire Detection

This article delves into the analysis of performance and utilization of Support Vector Machines (SVMs) for the critical task of forest fire detection using image datasets. With the increasing threat of forest fires to ecosystems and human settlements, the need for rapid and accurate detection systems is of utmost importance. SVMs, renowned for their strong classification capabilities, exhibit proficiency in recognizing patterns associated with fire within images. By training on labeled data, SVMs acquire the ability to identify distinctive attributes associated with fire, such as flames, smoke, or alterations in the visual characteristics of the forest area. The document thoroughly examines the use of SVMs, covering crucial elements like data preprocessing, feature extraction, and model training. It rigorously evaluates parameters such as accuracy, efficiency, and practical applicability. The knowledge gained from this study aids in the development of efficient forest fire detection systems, enabling prompt responses and improving disaster management. Moreover, the correlation between SVM accuracy and the difficulties presented by high-dimensional datasets is carefully investigated, demonstrated through a revealing case study. The relationship between accuracy scores and the different resolutions used for resizing the training datasets has also been discussed in this article. These comprehensive studies result in a definitive overview of the difficulties faced and the potential sectors requiring further improvement and focus.

Enhanced Steganalysis for Color Images Using Curvelet Features and Support Vector Machine

Algorithms for steganography are methods of hiding data transfers in media files.Several machine learning architectures have been presented recently to improve stego image identification performance by using spatial information,and these methods have made it feasible to handle a wide range of problems associated with image analysis.Images with little information or low payload are used by information embedding methods,but the goal of all contemporary research is to employ high-payload images for classification.To address the need for both low-and high-payload images,this work provides a machine-learning approach to steganography image classification that uses Curvelet transformation to efficiently extract characteristics from both type of images.Support Vector Machine(SVM),a commonplace classification technique,has been employed to determine whether the image is a stego or cover.The Wavelet Obtained Weights(WOW),Spatial Universal Wavelet Relative Distortion(S-UNIWARD),Highly Undetectable Steganography(HUGO),and Minimizing the Power of Optimal Detector(MiPOD)steganography techniques are used in a variety of experimental scenarios to evaluate the performance of the proposedmethod.Using WOW at several payloads,the proposed approach proves its classification accuracy of 98.60%.It exhibits its superiority over SOTA methods.

Spatial quantum coherent modulation with perfect hybrid vector vortex beam based on atomic medium

The perfect hybrid vector vortex beam(PHVVB)with helical phase wavefront structure has aroused significant concern in recent years,as its beam waist does not expand with the topological charge(TC).In this work,we investigate the spatial quantum coherent modulation effect with PHVVB based on the atomic medium,and we observe the absorption characteristic of the PHVVB with different TCs under variant magnetic fields.We find that the transmission spectrum linewidth of PHVVB can be effectively maintained regardless of the TC.Still,the width of transmission peaks increases slightly as the beam size expands in hot atomic vapor.This distinctive quantum coherence phenomenon,demonstrated by the interaction of an atomic medium with a hybrid vector-structured beam,might be anticipated to open up new opportunities for quantum coherence modulation and accurate magnetic field measurement.

Underwater Image Classification Based on EfficientnetB0 and Two-Hidden-Layer Random Vector Functional Link

The ocean plays an important role in maintaining the equilibrium of Earth’s ecology and providing humans access to a wealth of resources.To obtain a high-precision underwater image classification model,we propose a classification model that combines an EfficientnetB0 neural network and a two-hidden-layer random vector functional link network(EfficientnetB0-TRVFL).The features of underwater images were extracted using the EfficientnetB0 neural network pretrained via ImageNet,and a new fully connected layer was trained on the underwater image dataset using the transfer learning method.Transfer learning ensures the initial performance of the network and helps in the development of a high-precision classification model.Subsequently,a TRVFL was proposed to improve the classification property of the model.Net construction of the two hidden layers exhibited a high accuracy when the same hidden layer nodes were used.The parameters of the second hidden layer were obtained using a novel calculation method,which reduced the outcome error to improve the performance instability caused by the random generation of parameters of RVFL.Finally,the TRVFL classifier was used to classify features and obtain classification results.The proposed EfficientnetB0-TRVFL classification model achieved 87.28%,74.06%,and 99.59%accuracy on the MLC2008,MLC2009,and Fish-gres datasets,respectively.The best convolutional neural networks and existing methods were stacked up through box plots and Kolmogorov-Smirnov tests,respectively.The increases imply improved systematization properties in underwater image classification tasks.The image classification model offers important performance advantages and better stability compared with existing methods.

Prediction of Ground Vibration Induced by Rock Blasting Based on Optimized Support Vector Regression Models

Accurately estimating blasting vibration during rock blasting is the foundation of blasting vibration management.In this study,Tuna Swarm Optimization(TSO),Whale Optimization Algorithm(WOA),and Cuckoo Search(CS)were used to optimize two hyperparameters in support vector regression(SVR).Based on these methods,three hybrid models to predict peak particle velocity(PPV)for bench blasting were developed.Eighty-eight samples were collected to establish the PPV database,eight initial blasting parameters were chosen as input parameters for the predictionmodel,and the PPV was the output parameter.As predictive performance evaluation indicators,the coefficient of determination(R2),rootmean square error(RMSE),mean absolute error(MAE),and a10-index were selected.The normalizedmutual information value is then used to evaluate the impact of various input parameters on the PPV prediction outcomes.According to the research findings,TSO,WOA,and CS can all enhance the predictive performance of the SVR model.The TSO-SVR model provides the most accurate predictions.The performances of the optimized hybrid SVR models are superior to the unoptimized traditional prediction model.The maximum charge per delay impacts the PPV prediction value the most.

Secure Transmission of Compressed Medical Image Sequences on Communication Networks Using Motion Vector Watermarking

Medical imaging plays a key role within modern hospital management systems for diagnostic purposes.Compression methodologies are extensively employed to mitigate storage demands and enhance transmission speed,all while upholding image quality.Moreover,an increasing number of hospitals are embracing cloud computing for patient data storage,necessitating meticulous scrutiny of server security and privacy protocols.Nevertheless,considering the widespread availability of multimedia tools,the preservation of digital data integrity surpasses the significance of compression alone.In response to this concern,we propose a secure storage and transmission solution for compressed medical image sequences,such as ultrasound images,utilizing a motion vector watermarking scheme.The watermark is generated employing an error-correcting code known as Bose-Chaudhuri-Hocquenghem(BCH)and is subsequently embedded into the compressed sequence via block-based motion vectors.In the process of watermark embedding,motion vectors are selected based on their magnitude and phase angle.When embedding watermarks,no specific spatial area,such as a region of interest(ROI),is used in the images.The embedding of watermark bits is dependent on motion vectors.Although reversible watermarking allows the restoration of the original image sequences,we use the irreversible watermarking method.The reason for this is that the use of reversible watermarks may impede the claims of ownership and legal rights.The restoration of original data or images may call into question ownership or other legal claims.The peak signal-to-noise ratio(PSNR)and structural similarity index(SSIM)serve as metrics for evaluating the watermarked image quality.Across all images,the PSNR value exceeds 46 dB,and the SSIM value exceeds 0.92.Experimental results substantiate the efficacy of the proposed technique in preserving data integrity.

Comparison of debris flow susceptibility assessment methods:support vector machine,particle swarm optimization,and feature selection techniques

The selection of important factors in machine learning-based susceptibility assessments is crucial to obtain reliable susceptibility results.In this study,metaheuristic optimization and feature selection techniques were applied to identify the most important input parameters for mapping debris flow susceptibility in the southern mountain area of Chengde City in Hebei Province,China,by using machine learning algorithms.In total,133 historical debris flow records and 16 related factors were selected.The support vector machine(SVM)was first used as the base classifier,and then a hybrid model was introduced by a two-step process.First,the particle swarm optimization(PSO)algorithm was employed to select the SVM model hyperparameters.Second,two feature selection algorithms,namely principal component analysis(PCA)and PSO,were integrated into the PSO-based SVM model,which generated the PCA-PSO-SVM and FS-PSO-SVM models,respectively.Three statistical metrics(accuracy,recall,and specificity)and the area under the receiver operating characteristic curve(AUC)were employed to evaluate and validate the performance of the models.The results indicated that the feature selection-based models exhibited the best performance,followed by the PSO-based SVM and SVM models.Moreover,the performance of the FS-PSO-SVM model was better than that of the PCA-PSO-SVM model,showing the highest AUC,accuracy,recall,and specificity values in both the training and testing processes.It was found that the selection of optimal features is crucial to improving the reliability of debris flow susceptibility assessment results.Moreover,the PSO algorithm was found to be not only an effective tool for hyperparameter optimization,but also a useful feature selection algorithm to improve prediction accuracies of debris flow susceptibility by using machine learning algorithms.The high and very high debris flow susceptibility zone appropriately covers 38.01%of the study area,where debris flow may occur under intensive human activities and heavy rainfall events.

Whole Perfect Vectors and Fermat’s Last Theorem

A naïve discussion of Fermat’s last theorem conundrum is described. The present theorem’s proof is grounded on the well-known properties of sums of powers of the sine and cosine functions, the Minkowski norm definition, and some vector-specific structures.

Research on Node Classification Based on Joint Weighted Node Vectors

Node of network has lots of information, such as topology, text and label information. Therefore, node classification is an open issue. Recently, one vector of node is directly connected at the end of another vector. However, this method actually obtains the performance by extending dimensions and considering that the text and structural information are one-to-one, which is obviously unreasonable. Regarding this issue, a method by weighting vectors is proposed in this paper. Three methods, negative logarithm, modulus and sigmoid function are used to weight-trained vectors, then recombine the weighted vectors and put them into the SVM classifier for evaluation output. By comparing three different weighting methods, the results showed that using negative logarithm weighting achieved better results than the other two using modulus and sigmoid function weighting, and was superior to directly concatenating vectors in the same dimension.

Fully Distributed Learning for Deep Random Vector Functional-Link Networks

In the contemporary era, the proliferation of information technology has led to an unprecedented surge in data generation, with this data being dispersed across a multitude of mobile devices. Facing these situations and the training of deep learning model that needs great computing power support, the distributed algorithm that can carry out multi-party joint modeling has attracted everyone’s attention. The distributed training mode relieves the huge pressure of centralized model on computer computing power and communication. However, most distributed algorithms currently work in a master-slave mode, often including a central server for coordination, which to some extent will cause communication pressure, data leakage, privacy violations and other issues. To solve these problems, a decentralized fully distributed algorithm based on deep random weight neural network is proposed. The algorithm decomposes the original objective function into several sub-problems under consistency constraints, combines the decentralized average consensus (DAC) and alternating direction method of multipliers (ADMM), and achieves the goal of joint modeling and training through local calculation and communication of each node. Finally, we compare the proposed decentralized algorithm with several centralized deep neural networks with random weights, and experimental results demonstrate the effectiveness of the proposed algorithm.

Non-viral targeted delivery system mediates transfection of thymidine kinase gene of herpes simplex virus into ovarian cancer cells:a comparison between one time and continuous mediation

Objective: To compare the transferring efficiency and killing effects of one time and continuous mediation with GE7,a non-viral targeted delivery system,in transfection of thymidine kinase gene of herpes simplex virus (HSV-tk) into ovarian cancer cells. Methods: GE7 was used to prepare recombinants with β-galactosidase (β-gal) and HSV1-tk; the re-combinants were then used to transfect human ovarian cancer line CaOV3 once and continuously. β-gal staining was used to compare the efficiencies of one time and continuous mediation with GE7 system. Ganciclovior (GCV) was introduced into HSV1-tk transfected ovarian cells. Through drawing the cell growth curve and flow cytometry,the killing effects of GCV on once and continuously GE7/HSV1-tk transfected cells were observed. Results: We found that the one time and continuous exogenous gene transfer efficiencies were about 80% and 85%,respectively. When 1μg/mL GCV was used to treat ovarian cell transfected with HSV1-tk gene,growth inhibiting rates of ovarian cells of one time and continuous transferring were 82% and 90%,respectively; their apoptosis indices were 15 and 30,respectively. Under same GCV concentration,continuous me-diation of GE7/pCMV-tk transfection into ovarian cancer cells had more significant inhibitory effect than one time mediation (P<0.05). Conclusion: Compared with one time mediation,continuous mediation of transfection with GE7 gene delivery system has higher efficiency. Continuous mediation of GE7/HSV1-tk/GCV therapeutic gene system has more powerful killing effect.

Stability prediction of hard rock pillar using support vector machine optimized by three metaheuristic algorithms

Hard rock pillar is one of the important structures in engineering design and excavation in underground mines.Accurate and convenient prediction of pillar stability is of great significance for underground space safety.This paper aims to develop hybrid support vector machine(SVM)models improved by three metaheuristic algorithms known as grey wolf optimizer(GWO),whale optimization algorithm(WOA)and sparrow search algorithm(SSA)for predicting the hard rock pillar stability.An integrated dataset containing 306 hard rock pillars was established to generate hybrid SVM models.Five parameters including pillar height,pillar width,ratio of pillar width to height,uniaxial compressive strength and pillar stress were set as input parameters.Two global indices,three local indices and the receiver operating characteristic(ROC)curve with the area under the ROC curve(AUC)were utilized to evaluate all hybrid models’performance.The results confirmed that the SSA-SVM model is the best prediction model with the highest values of all global indices and local indices.Nevertheless,the performance of the SSASVM model for predicting the unstable pillar(AUC:0.899)is not as good as those for stable(AUC:0.975)and failed pillars(AUC:0.990).To verify the effectiveness of the proposed models,5 field cases were investigated in a metal mine and other 5 cases were collected from several published works.The validation results indicated that the SSA-SVM model obtained a considerable accuracy,which means that the combination of SVM and metaheuristic algorithms is a feasible approach to predict the pillar stability.