In conventional cross-coupled controller design,the method usually ignored the inherent characteristic of time-vary- ing parameters and model uncertainties in system.In this paper,a cross-coupled controller(CCC)using ...In conventional cross-coupled controller design,the method usually ignored the inherent characteristic of time-vary- ing parameters and model uncertainties in system.In this paper,a cross-coupled controller(CCC)using an H~∞control scheme has been proposed to reduce the contouring error for an X-Y table.Furthermore,the proposed CCC design,which is a typical Multi- Input Multi-Output(MIMO)system with linear time varying(LTV)characteristics,has been verified as being internally stable. The simulations are carried on Matlab simulink to verify the proposed method,and the results showed that the proposed method can reduce the contouring error significantly compared with the conventional one.展开更多
Based on Kunming statistical yearbook and field survey data of agricultural non-point source pollution in counties or districts in the Dianchi Lake basin,previous and current situation of agricultural production and a...Based on Kunming statistical yearbook and field survey data of agricultural non-point source pollution in counties or districts in the Dianchi Lake basin,previous and current situation of agricultural production and agricultural non-point source pollution control in the Dianchi Lake basin were studied,and countermeasures and suggestions of comprehensive control of agricultural non-point source pollution in the basin were proposed.展开更多
There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In ...There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In this study,we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes:Actb,Ankrd27,CypA,Gapdh,Hprt1,Mrpl10,Pgk1,Rictor,Rn18s,Tbp,Ubc,Ubxn11,and Ywhaz.Our results show that the expression of Ankrd27,Ubc,and Tbp were stable after spinal cord injury,while Actb was the most unstable internal control gene.Ankrd27,Ubc,Tbp,and Actb were consequently used to investigate the effects of internal control genes with differing stabilities on the normalization of target gene expression.Target gene expression levels and changes over time were similar when Ankrd27,Ubc,and Tbp were used as internal controls but different when Actb was used as an internal control.We recommend that Ankrd27,Ubc,and Tbp are used as internal control genes for real-time reverse transcription polymerase chain reaction in spinal cord injury research.This study was approved by the Administration Committee of Experimental Animals,Jiangsu Province,China(approval No.20180304-008)on March 4,2018.展开更多
Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and...Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and crossbred bulls.Methods:Total RNA was isolated from testicular tissue of Zebu and crossbred bulls(n=6 each)between 2-8 years of age.cDNA was synthesized,and the quantitative real-time polymerase chain reaction(PCR)was performed.The cycle threshold values were used for the analysis of the stability of ICGs.Four different statistical algorithms:geNorm,Normfinder,BestKeeper,and RefFinder,were used to assess the stability of these genes.Results:ATPSF1,HMBS,PPIA,and RPS15A were the most reliable and stable ICGs for Zebu testes,and ATPSF1,RPL23,and PPIA for crossbred testes.Conclusions:A panel of stable ICGs(ATPSF1,HMBS,PPIA,RPS15A for Zebu and ATPSF1,RPL23,and PPIA for crossbred)for normalization of gene expression data in testes samples can be helpful for researchers to conduct functional genomics studies at the testicular level in cattle bulls.展开更多
In this paper, a discussion is devoted to the theory and method of a reversed-frame normalization design to robust flight control system. The robust stability theory of the normal transfer function matrix with the sam...In this paper, a discussion is devoted to the theory and method of a reversed-frame normalization design to robust flight control system. The robust stability theory of the normal transfer function matrix with the same characteristic gain loci is proved. An example of flight control system design shows the application and advantage of this method.展开更多
Hyperimmune sera (HIS), raised against crude giardia antigen, on in vitro interaction, caused more agglutination of Giardia lamblia trophozoites. Heat inactivated HIS possessed a comparable agglutinating activity as t...Hyperimmune sera (HIS), raised against crude giardia antigen, on in vitro interaction, caused more agglutination of Giardia lamblia trophozoites. Heat inactivated HIS possessed a comparable agglutinating activity as the non-inactivated controls. Non-inactivated normal (unimmunized) serum caused immobilization of Giardia trophozoite, which was checked on heat inactivation. Antibodies in immune sera are mainly responsible for agglutination, whereas the heat labile non-immune components control the mobility of the intestinal parasite.展开更多
Relative radiometric normalization (RRN) minimizes radiometric differences among images caused by inconsistencies of acquisition conditions rather than changes in surface. Scale invariant feature transform (SIFT) has ...Relative radiometric normalization (RRN) minimizes radiometric differences among images caused by inconsistencies of acquisition conditions rather than changes in surface. Scale invariant feature transform (SIFT) has the ability to automatically extract control points (CPs) and is commonly used for remote sensing images. However, its results are mostly inaccurate and sometimes contain incorrect matching caused by generating a small number of false CP pairs. These CP pairs have high false alarm matching. This paper presents a modified method to improve the performance of SIFT CPs matching by applying sum of absolute difference (SAD) in a different manner for the new optical satellite generation called near-equatorial orbit satellite and multi-sensor images. The proposed method, which has a significantly high rate of correct matches, improves CP matching. The data in this study were obtained from the RazakSAT satellite a new near equatorial satellite system. The proposed method involves six steps: 1) data reduction, 2) applying the SIFT to automatically extract CPs, 3) refining CPs matching by using SAD algorithm with empirical threshold, and 4) calculation of true CPs intensity values over all image’ bands, 5) preforming a linear regression model between the intensity values of CPs locate in reverence and sensed image’ bands, 6) Relative radiometric normalization conducting using regression transformation functions. Different thresholds have experimentally tested and used in conducting this study (50 and 70), by followed the proposed method, and it removed the false extracted SIFT CPs to be from 775, 1125, 883, 804, 883 and 681 false pairs to 342, 424, 547, 706, 547, and 469 corrected and matched pairs, respectively.展开更多
冷热电联产(combined cooling, heating and power,CCHP)系统与微电网的结合有利于促进消纳可再生能源,为了提升CCHP型微电网的经济性、环保性和稳定性,提出了两阶段优化调度模型。离线优化阶段基于需求侧响应策略,建立了基于归一化法...冷热电联产(combined cooling, heating and power,CCHP)系统与微电网的结合有利于促进消纳可再生能源,为了提升CCHP型微电网的经济性、环保性和稳定性,提出了两阶段优化调度模型。离线优化阶段基于需求侧响应策略,建立了基于归一化法向约束法的多目标规划模型,并用熵权-TOPSIS法筛选最优结果。在线优化阶段建立了基于动态矩阵控制算法的有限时域优化模型,对离线优化结果进行跟踪优化和反馈校正,以降低不确定性因素的影响。最后,设计对比方案进行分析,验证了所提优化模型的有效性。展开更多
The selective coupling of alcohols is a fascinating yet challenging approach for upgrading alcohols. Herein, we accomplished the controlled production of β-disubstituted ketones or upgraded secondary alcohols via the...The selective coupling of alcohols is a fascinating yet challenging approach for upgrading alcohols. Herein, we accomplished the controlled production of β-disubstituted ketones or upgraded secondary alcohols via the Ir-catalyzed cross-coupling of secondary alcohols in excellent yields with broad substrate scopes. This selective control was achieved by using an in-situ generated monoNHC-Ir or a tris-NHC-Ir complex as the catalysts, respectively. Mechanistic studies revealed that the delicate balance between dehydrogenation and hydrogenation abilities of these bifunctional catalysts is crucial for achieving different selectivity. The tris-NHC-Ir complex effectively facilitated dehydrogenation of alcohols and hydrogenation of intermediates, leading to the desired upgraded secondary alcohols. Conversely, the high dehydrogenation ability of the mono-NHC-Ir complex promoted the conversion of formed secondary alcohols back to ketones.展开更多
Neuroimaging has emerged over the last few decades as a crucial tool in diagnosing Alzheimer’s disease(AD).Mild cognitive impairment(MCI)is a condition that falls between the spectrum of normal cognitive function and...Neuroimaging has emerged over the last few decades as a crucial tool in diagnosing Alzheimer’s disease(AD).Mild cognitive impairment(MCI)is a condition that falls between the spectrum of normal cognitive function and AD.However,previous studies have mainly used handcrafted features to classify MCI,AD,and normal control(NC)individuals.This paper focuses on using gray matter(GM)scans obtained through magnetic resonance imaging(MRI)for the diagnosis of individuals with MCI,AD,and NC.To improve classification performance,we developed two transfer learning strategies with data augmentation(i.e.,shear range,rotation,zoom range,channel shift).The first approach is a deep Siamese network(DSN),and the second approach involves using a cross-domain strategy with customized VGG-16.We performed experiments on the Alzheimer’s Disease Neuroimaging Initiative(ADNI)dataset to evaluate the performance of our proposed models.Our experimental results demonstrate superior performance in classifying the three binary classification tasks:NC vs.AD,NC vs.MCI,and MCI vs.AD.Specifically,we achieved a classification accuracy of 97.68%,94.25%,and 92.18%for the three cases,respectively.Our study proposes two transfer learning strategies with data augmentation to accurately diagnose MCI,AD,and normal control individuals using GM scans.Our findings provide promising results for future research and clinical applications in the early detection and diagnosis of AD.展开更多
文摘In conventional cross-coupled controller design,the method usually ignored the inherent characteristic of time-vary- ing parameters and model uncertainties in system.In this paper,a cross-coupled controller(CCC)using an H~∞control scheme has been proposed to reduce the contouring error for an X-Y table.Furthermore,the proposed CCC design,which is a typical Multi- Input Multi-Output(MIMO)system with linear time varying(LTV)characteristics,has been verified as being internally stable. The simulations are carried on Matlab simulink to verify the proposed method,and the results showed that the proposed method can reduce the contouring error significantly compared with the conventional one.
基金Supported by Research on the Comprehensive Control Measures of Agricultural Non-point Source Pollution in the Dianchi Lake Basin Funded by Kunming Agricultural Bureau(2016JC01)Project of Yunnan Engineering Research Center for Urban Characteristic Agriculture(TSNY0201)+7 种基金Scientific Research Foundation of Yunnan Department of Education(2014Y390)Application Base Research Project for Young Scholars of Yunnan Province(2013FD040)Talent Introduction Project of Kunming University(YJL14005)Talent Introduction Foundation of Kunming University(YJL12010)Key Foundation of Kunming University(XJL12020)Scientific Research Project for Undergraduates of Kunming University(XJD16081)Research on the Ecology and Rational Distribution of Main Cultivars in Producing Areas of Raw Materials of China Tobacco in Yunnan ProvinceProject of Construction of the Dominant Key Subject(Ecology)in Universities and Colleges in Yunnan Province(05000511311)
文摘Based on Kunming statistical yearbook and field survey data of agricultural non-point source pollution in counties or districts in the Dianchi Lake basin,previous and current situation of agricultural production and agricultural non-point source pollution control in the Dianchi Lake basin were studied,and countermeasures and suggestions of comprehensive control of agricultural non-point source pollution in the basin were proposed.
基金the National Natural Science Foundation of China,No.81901257(to YXW)the Natural Science Foundation of Jiangsu Province of China,No.BK20180951(to YXW)+1 种基金Postgraduate Research and Practice Innovation Program of Jiangsu Province of China,No.KYCX20_2818(to WL)and Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD,to Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education).
文摘There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In this study,we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes:Actb,Ankrd27,CypA,Gapdh,Hprt1,Mrpl10,Pgk1,Rictor,Rn18s,Tbp,Ubc,Ubxn11,and Ywhaz.Our results show that the expression of Ankrd27,Ubc,and Tbp were stable after spinal cord injury,while Actb was the most unstable internal control gene.Ankrd27,Ubc,Tbp,and Actb were consequently used to investigate the effects of internal control genes with differing stabilities on the normalization of target gene expression.Target gene expression levels and changes over time were similar when Ankrd27,Ubc,and Tbp were used as internal controls but different when Actb was used as an internal control.We recommend that Ankrd27,Ubc,and Tbp are used as internal control genes for real-time reverse transcription polymerase chain reaction in spinal cord injury research.This study was approved by the Administration Committee of Experimental Animals,Jiangsu Province,China(approval No.20180304-008)on March 4,2018.
基金The present study was carried out under the project“Molecular markers for improving reproduction in cattle and buffaloes”under the funding of Bill and Melinda Gates Foundation,USA and Indian Council of Agricultural Research-National Dairy Research Institute.
文摘Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and crossbred bulls.Methods:Total RNA was isolated from testicular tissue of Zebu and crossbred bulls(n=6 each)between 2-8 years of age.cDNA was synthesized,and the quantitative real-time polymerase chain reaction(PCR)was performed.The cycle threshold values were used for the analysis of the stability of ICGs.Four different statistical algorithms:geNorm,Normfinder,BestKeeper,and RefFinder,were used to assess the stability of these genes.Results:ATPSF1,HMBS,PPIA,and RPS15A were the most reliable and stable ICGs for Zebu testes,and ATPSF1,RPL23,and PPIA for crossbred testes.Conclusions:A panel of stable ICGs(ATPSF1,HMBS,PPIA,RPS15A for Zebu and ATPSF1,RPL23,and PPIA for crossbred)for normalization of gene expression data in testes samples can be helpful for researchers to conduct functional genomics studies at the testicular level in cattle bulls.
基金This paper has been supported by the National Natural Science Foundation of Chaina.
文摘In this paper, a discussion is devoted to the theory and method of a reversed-frame normalization design to robust flight control system. The robust stability theory of the normal transfer function matrix with the same characteristic gain loci is proved. An example of flight control system design shows the application and advantage of this method.
文摘Hyperimmune sera (HIS), raised against crude giardia antigen, on in vitro interaction, caused more agglutination of Giardia lamblia trophozoites. Heat inactivated HIS possessed a comparable agglutinating activity as the non-inactivated controls. Non-inactivated normal (unimmunized) serum caused immobilization of Giardia trophozoite, which was checked on heat inactivation. Antibodies in immune sera are mainly responsible for agglutination, whereas the heat labile non-immune components control the mobility of the intestinal parasite.
文摘Relative radiometric normalization (RRN) minimizes radiometric differences among images caused by inconsistencies of acquisition conditions rather than changes in surface. Scale invariant feature transform (SIFT) has the ability to automatically extract control points (CPs) and is commonly used for remote sensing images. However, its results are mostly inaccurate and sometimes contain incorrect matching caused by generating a small number of false CP pairs. These CP pairs have high false alarm matching. This paper presents a modified method to improve the performance of SIFT CPs matching by applying sum of absolute difference (SAD) in a different manner for the new optical satellite generation called near-equatorial orbit satellite and multi-sensor images. The proposed method, which has a significantly high rate of correct matches, improves CP matching. The data in this study were obtained from the RazakSAT satellite a new near equatorial satellite system. The proposed method involves six steps: 1) data reduction, 2) applying the SIFT to automatically extract CPs, 3) refining CPs matching by using SAD algorithm with empirical threshold, and 4) calculation of true CPs intensity values over all image’ bands, 5) preforming a linear regression model between the intensity values of CPs locate in reverence and sensed image’ bands, 6) Relative radiometric normalization conducting using regression transformation functions. Different thresholds have experimentally tested and used in conducting this study (50 and 70), by followed the proposed method, and it removed the false extracted SIFT CPs to be from 775, 1125, 883, 804, 883 and 681 false pairs to 342, 424, 547, 706, 547, and 469 corrected and matched pairs, respectively.
文摘冷热电联产(combined cooling, heating and power,CCHP)系统与微电网的结合有利于促进消纳可再生能源,为了提升CCHP型微电网的经济性、环保性和稳定性,提出了两阶段优化调度模型。离线优化阶段基于需求侧响应策略,建立了基于归一化法向约束法的多目标规划模型,并用熵权-TOPSIS法筛选最优结果。在线优化阶段建立了基于动态矩阵控制算法的有限时域优化模型,对离线优化结果进行跟踪优化和反馈校正,以降低不确定性因素的影响。最后,设计对比方案进行分析,验证了所提优化模型的有效性。
基金supported by the National Natural Science Foundation of China(22271060)the Department of Chemistry at Fudan University。
文摘The selective coupling of alcohols is a fascinating yet challenging approach for upgrading alcohols. Herein, we accomplished the controlled production of β-disubstituted ketones or upgraded secondary alcohols via the Ir-catalyzed cross-coupling of secondary alcohols in excellent yields with broad substrate scopes. This selective control was achieved by using an in-situ generated monoNHC-Ir or a tris-NHC-Ir complex as the catalysts, respectively. Mechanistic studies revealed that the delicate balance between dehydrogenation and hydrogenation abilities of these bifunctional catalysts is crucial for achieving different selectivity. The tris-NHC-Ir complex effectively facilitated dehydrogenation of alcohols and hydrogenation of intermediates, leading to the desired upgraded secondary alcohols. Conversely, the high dehydrogenation ability of the mono-NHC-Ir complex promoted the conversion of formed secondary alcohols back to ketones.
基金Research work funded by Zhejiang Normal University Research Fund YS304023947 and YS304023948.
文摘Neuroimaging has emerged over the last few decades as a crucial tool in diagnosing Alzheimer’s disease(AD).Mild cognitive impairment(MCI)is a condition that falls between the spectrum of normal cognitive function and AD.However,previous studies have mainly used handcrafted features to classify MCI,AD,and normal control(NC)individuals.This paper focuses on using gray matter(GM)scans obtained through magnetic resonance imaging(MRI)for the diagnosis of individuals with MCI,AD,and NC.To improve classification performance,we developed two transfer learning strategies with data augmentation(i.e.,shear range,rotation,zoom range,channel shift).The first approach is a deep Siamese network(DSN),and the second approach involves using a cross-domain strategy with customized VGG-16.We performed experiments on the Alzheimer’s Disease Neuroimaging Initiative(ADNI)dataset to evaluate the performance of our proposed models.Our experimental results demonstrate superior performance in classifying the three binary classification tasks:NC vs.AD,NC vs.MCI,and MCI vs.AD.Specifically,we achieved a classification accuracy of 97.68%,94.25%,and 92.18%for the three cases,respectively.Our study proposes two transfer learning strategies with data augmentation to accurately diagnose MCI,AD,and normal control individuals using GM scans.Our findings provide promising results for future research and clinical applications in the early detection and diagnosis of AD.