This paper aims at providing a scientific basis for unifying the normal reference value standards of red blood cell count of Chinese presenile men. The paper, using microscopical counting method, studies the relations...This paper aims at providing a scientific basis for unifying the normal reference value standards of red blood cell count of Chinese presenile men. The paper, using microscopical counting method, studies the relationship between the normal reference values of 38,061 samples of red blood cell count of presenile men and eight geographical factors in 297 units in China. It is found that the correlation of geographical factors and the normal reference value of red blood cell count of presenile men is quite significant (F=303.00, P=0.000). By using the method of stepwise regression analysis, one regression equation is inferred. It is concluded that if geographical data are obtained in a certain area, the normal reference value of red blood cell count of presenile men in this area can be reckoned by using the regression analysis. Furthermore, according to the geographical factors, China can be divided into eight regions: Northeast China Region, North China Region, Shanxi-Shaanxi-Inner Mongolia Region, Middle and Lower Reaches of the Changjiang River Region, Southeast China Region, Northwest China Region, Southwest China Region and Qinghai-Tibet Plateau Region.展开更多
The changes of blood perfusion and oxygen transport in tumors during tumor vascular normalization are studied with 3-dimensional mathematical modeling and numerical simulation. The models of tumor angiogenesis and vas...The changes of blood perfusion and oxygen transport in tumors during tumor vascular normalization are studied with 3-dimensional mathematical modeling and numerical simulation. The models of tumor angiogenesis and vascular-disrupting are used to simulate ＂un-normalized＂ and ＂normalized＂ vasculatures. A new model combining tumor hemodynamics and oxygen transport is developed. In this model, the intravasculartransvascular-interstitial flow with red blood cell（RBC） delivery is tightly coupled, and the oxygen resource is produced by heterogeneous distribution of hematocrit from the flow simulation. The results show that both tumor blood perfusion and hematocrit in the vessels increase, and the hypoxia microenvironment in the tumor center is greatly improved during vascular normalization. The total oxygen content inside the tumor tissue increases by about 67%, 51%, and 95% for the three approaches of vascular normalization,respectively. The elevation of oxygen concentration in tumors can improve its metabolic environment, and consequently reduce malignancy of tumor cells. It can also enhance radiation and chemotherapeutics to tumors.展开更多
目的：探讨直接抗人球蛋白试验（DAT）阳性患者的交叉配血方法。方法采用 DAT 法筛查红细胞表面的不规则抗体，微柱凝胶法进行血型鉴定，凝聚胺法和抗人球蛋白法交叉配血。结果共计5例 DAT 阳性的疑难配血患者，其血型分别为 A-DCcEe，O...目的：探讨直接抗人球蛋白试验（DAT）阳性患者的交叉配血方法。方法采用 DAT 法筛查红细胞表面的不规则抗体，微柱凝胶法进行血型鉴定，凝聚胺法和抗人球蛋白法交叉配血。结果共计5例 DAT 阳性的疑难配血患者，其血型分别为 A-DCcEe，O-DCCee，O-DCcee，A-DCCee 和 A-DCcee。选择相应血型抗原阴性的供者血样进行交叉配血，凝集强度均弱于自身对照，建议观察输注洗涤红细胞，患者未发生不良反应。结论对于 DAT 阳性的患者，可配合性输注相应血型抗原阴性的洗涤红细胞。展开更多
目的探究在冰冻红细胞解冻洗涤液中添加适宜浓度的木糖醇,提高解冻红细胞回收率的可行性。方法选择6 d内制备的400 ml去白细胞悬浮红细胞20袋,离心去浆后分装等量(100 ml)浓缩红细胞作为实验组和对照组,实验组在解冻第1次洗涤液(80 ml 9...目的探究在冰冻红细胞解冻洗涤液中添加适宜浓度的木糖醇,提高解冻红细胞回收率的可行性。方法选择6 d内制备的400 ml去白细胞悬浮红细胞20袋,离心去浆后分装等量(100 ml)浓缩红细胞作为实验组和对照组,实验组在解冻第1次洗涤液(80 ml 9%Na Cl)和第2次洗涤液(200 ml 0.9%Na Cl)中分别加入终浓度为1.2%、0.2%的木糖醇,比较两组的RBC回收率、红细胞渗透脆性、甘油残留量,同时测定冰冻红细胞解冻洗涤过程中各阶段的游离血红蛋白含量,明确提高解冻红细胞回收率的关键步骤。结果实验组RBC回收率显著高于对照组(t=-10.222,P<0.05),而红细胞渗透脆性、甘油残留量与对照组相比差异无统计学意义(P>0.05)。解冻第1次洗涤后血浆游离血红蛋白含量以实验组显著低于对照组(t=24.496,P<0.05),其余洗涤步骤的差异则无统计学意义(P>0.05)。结论冰冻红细胞经第1次洗涤液(80 ml 9%Na Cl)的解冻洗涤过程为RBC回收率提高的关键步骤。展开更多
基金Under the auspices of the National Natural Science Foundation of China (No. 40371004)
文摘This paper aims at providing a scientific basis for unifying the normal reference value standards of red blood cell count of Chinese presenile men. The paper, using microscopical counting method, studies the relationship between the normal reference values of 38,061 samples of red blood cell count of presenile men and eight geographical factors in 297 units in China. It is found that the correlation of geographical factors and the normal reference value of red blood cell count of presenile men is quite significant (F=303.00, P=0.000). By using the method of stepwise regression analysis, one regression equation is inferred. It is concluded that if geographical data are obtained in a certain area, the normal reference value of red blood cell count of presenile men in this area can be reckoned by using the regression analysis. Furthermore, according to the geographical factors, China can be divided into eight regions: Northeast China Region, North China Region, Shanxi-Shaanxi-Inner Mongolia Region, Middle and Lower Reaches of the Changjiang River Region, Southeast China Region, Northwest China Region, Southwest China Region and Qinghai-Tibet Plateau Region.
基金Project supported by the National Natural Science Foundation of China(Nos.11102113 and81301816)the New Teachers Start Program of Shanghai Jiao Tong University+1 种基金the Chenxing Young Scholars Program B of Shanghai Jiao Tong University(No.13X100010070)the Natural Science Research Foundation of Shanghai Jiao Tong University School of Medicine(No.13XJ10037)
文摘The changes of blood perfusion and oxygen transport in tumors during tumor vascular normalization are studied with 3-dimensional mathematical modeling and numerical simulation. The models of tumor angiogenesis and vascular-disrupting are used to simulate ＂un-normalized＂ and ＂normalized＂ vasculatures. A new model combining tumor hemodynamics and oxygen transport is developed. In this model, the intravasculartransvascular-interstitial flow with red blood cell（RBC） delivery is tightly coupled, and the oxygen resource is produced by heterogeneous distribution of hematocrit from the flow simulation. The results show that both tumor blood perfusion and hematocrit in the vessels increase, and the hypoxia microenvironment in the tumor center is greatly improved during vascular normalization. The total oxygen content inside the tumor tissue increases by about 67%, 51%, and 95% for the three approaches of vascular normalization,respectively. The elevation of oxygen concentration in tumors can improve its metabolic environment, and consequently reduce malignancy of tumor cells. It can also enhance radiation and chemotherapeutics to tumors.
文摘目的：探讨直接抗人球蛋白试验（DAT）阳性患者的交叉配血方法。方法采用 DAT 法筛查红细胞表面的不规则抗体，微柱凝胶法进行血型鉴定，凝聚胺法和抗人球蛋白法交叉配血。结果共计5例 DAT 阳性的疑难配血患者，其血型分别为 A-DCcEe，O-DCCee，O-DCcee，A-DCCee 和 A-DCcee。选择相应血型抗原阴性的供者血样进行交叉配血，凝集强度均弱于自身对照，建议观察输注洗涤红细胞，患者未发生不良反应。结论对于 DAT 阳性的患者，可配合性输注相应血型抗原阴性的洗涤红细胞。
文摘目的探究在冰冻红细胞解冻洗涤液中添加适宜浓度的木糖醇,提高解冻红细胞回收率的可行性。方法选择6 d内制备的400 ml去白细胞悬浮红细胞20袋,离心去浆后分装等量(100 ml)浓缩红细胞作为实验组和对照组,实验组在解冻第1次洗涤液(80 ml 9%Na Cl)和第2次洗涤液(200 ml 0.9%Na Cl)中分别加入终浓度为1.2%、0.2%的木糖醇,比较两组的RBC回收率、红细胞渗透脆性、甘油残留量,同时测定冰冻红细胞解冻洗涤过程中各阶段的游离血红蛋白含量,明确提高解冻红细胞回收率的关键步骤。结果实验组RBC回收率显著高于对照组(t=-10.222,P<0.05),而红细胞渗透脆性、甘油残留量与对照组相比差异无统计学意义(P>0.05)。解冻第1次洗涤后血浆游离血红蛋白含量以实验组显著低于对照组(t=24.496,P<0.05),其余洗涤步骤的差异则无统计学意义(P>0.05)。结论冰冻红细胞经第1次洗涤液(80 ml 9%Na Cl)的解冻洗涤过程为RBC回收率提高的关键步骤。
