A simple method for calculating the 3D coordinates of points on a micro object in a multi camera system is proposed. It simplifies the algorithms used in traditional computer vision system by eliminating the calculat...A simple method for calculating the 3D coordinates of points on a micro object in a multi camera system is proposed. It simplifies the algorithms used in traditional computer vision system by eliminating the calculation of the CCD (charge coupled device) camera parameters and the relative position between cameras, and using solid geometry in the calculation procedures instead of the calculation of the complex matrixes. The algorithm was used in the research of generating a virtual magnified 3D image of a micro object to be operated in a micro operation system, and the satisfactory results were obtained. The application in a virtual tele operation system for a dexterous mechanical gripper is under test.展开更多
Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio(SNR)greatly[C.J.R.Sheppard,"Super resolution in confocal imaging,&quo...Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio(SNR)greatly[C.J.R.Sheppard,"Super resolution in confocal imaging,"Optik 80(2)53-54(1988).C.B.Miller,E.Jorg,"Image scanning microscopy,"Phys.Reu.Lett.104(19)198101(2010).C.J.R.Sheppard,s.B.Mehta,R Heintzmann,"Superresolution by image scanning microscopy using pixel reassignment,"Opt.Lett.38(15)28892892(2013)].Here,we use a tailor-made optical fiber and 19 avalanche pho-todiodes(APDs)as parallel detectors to upgrade our existing confocal microscopy,termed as parallel-detection super resolution(PDSR)microscopy.In order to obtain the correct shift value,we use the normalized 2D cross correlation to calculate the shifting value of each image.We characterized our system performance by imaging fuorescence beads and applied this system to observing the 3D structure of biological specimen.展开更多
文摘A simple method for calculating the 3D coordinates of points on a micro object in a multi camera system is proposed. It simplifies the algorithms used in traditional computer vision system by eliminating the calculation of the CCD (charge coupled device) camera parameters and the relative position between cameras, and using solid geometry in the calculation procedures instead of the calculation of the complex matrixes. The algorithm was used in the research of generating a virtual magnified 3D image of a micro object to be operated in a micro operation system, and the satisfactory results were obtained. The application in a virtual tele operation system for a dexterous mechanical gripper is under test.
基金sponsored by National Natural Science Foundation of China(61827825 and 61735017)Fundamental Research Funds for the Central Universities(2019XZZX003-06)+1 种基金Natural Science Foundation of Zhejiang province(LR16F050001)Zhejiang Lab(2018EB0ZX01).
文摘Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio(SNR)greatly[C.J.R.Sheppard,"Super resolution in confocal imaging,"Optik 80(2)53-54(1988).C.B.Miller,E.Jorg,"Image scanning microscopy,"Phys.Reu.Lett.104(19)198101(2010).C.J.R.Sheppard,s.B.Mehta,R Heintzmann,"Superresolution by image scanning microscopy using pixel reassignment,"Opt.Lett.38(15)28892892(2013)].Here,we use a tailor-made optical fiber and 19 avalanche pho-todiodes(APDs)as parallel detectors to upgrade our existing confocal microscopy,termed as parallel-detection super resolution(PDSR)microscopy.In order to obtain the correct shift value,we use the normalized 2D cross correlation to calculate the shifting value of each image.We characterized our system performance by imaging fuorescence beads and applied this system to observing the 3D structure of biological specimen.
