Coronavirus disease(COVID-19)is a serious respiratory disease that spreads through the coronavirus globally.It soon became a pandemic after its appearance in 2019 and demanded new techniques for its identification and...Coronavirus disease(COVID-19)is a serious respiratory disease that spreads through the coronavirus globally.It soon became a pandemic after its appearance in 2019 and demanded new techniques for its identification and detection.Owing to this situation,RT-LAMP appears to be a novel method for the identification of COVID-19 because of its vast applications,including cost-effectiveness and time-saving.This research highlights the use of RT-LAMP,a more sensitive test than RT-PCR,for the assessment of SARS-CoV-2,the severe acute respiratory illness.To identify the spike(S)and NSP1 protein using RT-LAMP,170 total samples of coronavirus-suspected patients were served in this research.Health certifications and bioethical considerations were taken into consideration.After the sample was extracted from the patient's swabs,RNA was isolated,extracted,and purified.The response was then run on the RT-LAMP at the ideal temperature,and the outcomes could be observed with the unaided eye as they changed from pink to yellow.It is a simple method of determining if the test is positive or negative.For this purpose,both RT-LAMP and RT-PCR tests are used during these procedures.Genes linked with COVID-19 testing including S,nspl,and ORF are suited to coronavirus testing;they have 100%specificity and low sensitivity,but S has more specificity and sensitivity than nspl and ORF,respectively.Out of the 95 positive samples,89(93.68%)samples yielded favorable outcomes utilizing RT-LAMP,while 55 negative samples yielded 100%positive results.The present research demonstrates that RT-LAMP is less sensitive yet more selective for coronavirus detection.展开更多
轮状病毒(rotavirus,RV)非结构蛋白1(nonstructural protein 1,NSP1)是轮状病毒逃避宿主天然免疫应答的关键蛋白质。它可以与干扰素调控因子家族(interferon regulatoryfactor family,IRFF)的共同区域结合,阻断干扰素表达的信号通路,降...轮状病毒(rotavirus,RV)非结构蛋白1(nonstructural protein 1,NSP1)是轮状病毒逃避宿主天然免疫应答的关键蛋白质。它可以与干扰素调控因子家族(interferon regulatoryfactor family,IRFF)的共同区域结合,阻断干扰素表达的信号通路,降低宿主细胞I型干扰素(type I interferon,IFN-I)的表达,从而抑制宿主天然抗病毒免疫机制的建立。因此,NSP1被认为是轮状病毒的一种重要毒力因子。本文综述了近年来轮状病毒NSP1与宿主相互作用的研究进展。展开更多
文摘Coronavirus disease(COVID-19)is a serious respiratory disease that spreads through the coronavirus globally.It soon became a pandemic after its appearance in 2019 and demanded new techniques for its identification and detection.Owing to this situation,RT-LAMP appears to be a novel method for the identification of COVID-19 because of its vast applications,including cost-effectiveness and time-saving.This research highlights the use of RT-LAMP,a more sensitive test than RT-PCR,for the assessment of SARS-CoV-2,the severe acute respiratory illness.To identify the spike(S)and NSP1 protein using RT-LAMP,170 total samples of coronavirus-suspected patients were served in this research.Health certifications and bioethical considerations were taken into consideration.After the sample was extracted from the patient's swabs,RNA was isolated,extracted,and purified.The response was then run on the RT-LAMP at the ideal temperature,and the outcomes could be observed with the unaided eye as they changed from pink to yellow.It is a simple method of determining if the test is positive or negative.For this purpose,both RT-LAMP and RT-PCR tests are used during these procedures.Genes linked with COVID-19 testing including S,nspl,and ORF are suited to coronavirus testing;they have 100%specificity and low sensitivity,but S has more specificity and sensitivity than nspl and ORF,respectively.Out of the 95 positive samples,89(93.68%)samples yielded favorable outcomes utilizing RT-LAMP,while 55 negative samples yielded 100%positive results.The present research demonstrates that RT-LAMP is less sensitive yet more selective for coronavirus detection.
文摘轮状病毒(rotavirus,RV)非结构蛋白1(nonstructural protein 1,NSP1)是轮状病毒逃避宿主天然免疫应答的关键蛋白质。它可以与干扰素调控因子家族(interferon regulatoryfactor family,IRFF)的共同区域结合,阻断干扰素表达的信号通路,降低宿主细胞I型干扰素(type I interferon,IFN-I)的表达,从而抑制宿主天然抗病毒免疫机制的建立。因此,NSP1被认为是轮状病毒的一种重要毒力因子。本文综述了近年来轮状病毒NSP1与宿主相互作用的研究进展。
