AIM To investigate the interactions of the DNA repair gene excision repair cross complementing group 5(ERCC5) and the metabolic gene glutathione S-transferase pi 1(GSTP1) and their effects on atrophic gastritis(AG) an...AIM To investigate the interactions of the DNA repair gene excision repair cross complementing group 5(ERCC5) and the metabolic gene glutathione S-transferase pi 1(GSTP1) and their effects on atrophic gastritis(AG) and gastric cancer(GC) risk.METHODS Seven ERCC5 single nucleotide polymorphisms(SNPs)(rs1047768, rs2094258, rs2228959, rs4150291, rs4150383, rs751402, and rs873601) and GSTP1 SNP rs1695 were detected using the Sequenom MassA RRAY platform in 450 GC patients, 634 AG cases, and 621 healthy control subjects in a Chinese population.RESULTS Two pairwise combinations(ERCC5 rs2094258 and rs873601 with GSTP1 rs1695) influenced AG risk(P_(interaction) = 0.008 and 0.043, respectively), and the ERCC5 rs2094258-GSTP1 rs1695 SNP pair demonstrated an antagonistic effect, while ERCC5 rs873601-GSTP1 rs1695 showed a synergistic effect on AG risk OR = 0.51 and 1.79, respectively). No pairwise combinations were observed in relation to GC risk. There were no cumulative effects among the pairwise interactions(ERCC5 rs2094258 and rs873601 with GSTP1 rs1695) on AG susceptibility(P_(trend) > 0.05). When the modification effect of Helicobacter pylori(H. pylori) infection was evaluated, the cumulative effect of one of the aforementioned pairwise interactions(ERCC5 rs873601-GSTP1 rs1695) was associated with an increased AG risk in the case of negative H. pylori status(P_(trend)= 0.043).CONCLUSION There is a multifarious interaction between the DNA repair gene ERCC5 SNPs(rs2094258 and rs873601) and the metabolic gene GSTP1 rs1695, which may form the basis for various inter-individual susceptibilities to AG.展开更多
To assses the effect of single nucleotide polymorphism of excision repair cross-complementation group 1 C8092A on the clinical outcome and toxicity in advanced stage non-small cell lung cancer patients receiving first...To assses the effect of single nucleotide polymorphism of excision repair cross-complementation group 1 C8092A on the clinical outcome and toxicity in advanced stage non-small cell lung cancer patients receiving first line platinum based chemotherapy.MethodsThis article is a review of the current research on single nucleotide polymorphism and its effect on treatment outcome and toxicity of advanced stage lung cancer.Conclusion The observations indicate that more advanced studies and trials on C8092A SNPs are needed so as to assess if it could be used as a potential biomarker in the future.展开更多
Objective:To study the correlation of ERCC1 and GSTP1 expression in esophageal cancer tissue with platinum-based chemotherapy sensitivity as well as apoptosis and proliferation gene expression.Methods: Patients with a...Objective:To study the correlation of ERCC1 and GSTP1 expression in esophageal cancer tissue with platinum-based chemotherapy sensitivity as well as apoptosis and proliferation gene expression.Methods: Patients with advanced esophageal cancer who accepted PF chemotherapy in our hospital between May 2013 and October 2015 were selected, esophageal cancer tissue was collected before the chemotherapy, the patients were divided into chemotherapy sensitivity group and chemotherapy resistance group according to the effect of chemotherapy, and the expression levels of ERCC1, GSTP1 as well as apoptosis and proliferation genes in esophageal cancer tissue were detected.Results:Protein content and positive protein expression rate of ERCC1 and GSTP1 in esophageal cancer tissue of chemotherapy sensitivity group were significantly lower than those of chemotherapy resistance group, MBP1, DEC1 and PTEN protein content were significantly higher than those of chemotherapy resistance group, and PLCE1, CyclinD1 and PAR2 protein content were significantly lower than those of chemotherapy resistance group;MBP1, DEC1 and PTEN protein content in esophageal cancer tissue with positive ERCC1 and GSTP1 expression were significantly lower than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression while PLCE1, CyclinD1 and PAR2 protein content were significantly higher than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression.Conclusion:The highly expressed ERCC1 and GSTP1 in esophageal cancer tissue can decreased the cancer cell sensitivity to platinum-based chemotherapeutics, inhibit cell apoptosis and promote cell proliferation during platinum-based chemotherapy.展开更多
基金Supported by the National Science and Technology Support Program,No.2015BAI13B07
文摘AIM To investigate the interactions of the DNA repair gene excision repair cross complementing group 5(ERCC5) and the metabolic gene glutathione S-transferase pi 1(GSTP1) and their effects on atrophic gastritis(AG) and gastric cancer(GC) risk.METHODS Seven ERCC5 single nucleotide polymorphisms(SNPs)(rs1047768, rs2094258, rs2228959, rs4150291, rs4150383, rs751402, and rs873601) and GSTP1 SNP rs1695 were detected using the Sequenom MassA RRAY platform in 450 GC patients, 634 AG cases, and 621 healthy control subjects in a Chinese population.RESULTS Two pairwise combinations(ERCC5 rs2094258 and rs873601 with GSTP1 rs1695) influenced AG risk(P_(interaction) = 0.008 and 0.043, respectively), and the ERCC5 rs2094258-GSTP1 rs1695 SNP pair demonstrated an antagonistic effect, while ERCC5 rs873601-GSTP1 rs1695 showed a synergistic effect on AG risk OR = 0.51 and 1.79, respectively). No pairwise combinations were observed in relation to GC risk. There were no cumulative effects among the pairwise interactions(ERCC5 rs2094258 and rs873601 with GSTP1 rs1695) on AG susceptibility(P_(trend) > 0.05). When the modification effect of Helicobacter pylori(H. pylori) infection was evaluated, the cumulative effect of one of the aforementioned pairwise interactions(ERCC5 rs873601-GSTP1 rs1695) was associated with an increased AG risk in the case of negative H. pylori status(P_(trend)= 0.043).CONCLUSION There is a multifarious interaction between the DNA repair gene ERCC5 SNPs(rs2094258 and rs873601) and the metabolic gene GSTP1 rs1695, which may form the basis for various inter-individual susceptibilities to AG.
文摘To assses the effect of single nucleotide polymorphism of excision repair cross-complementation group 1 C8092A on the clinical outcome and toxicity in advanced stage non-small cell lung cancer patients receiving first line platinum based chemotherapy.MethodsThis article is a review of the current research on single nucleotide polymorphism and its effect on treatment outcome and toxicity of advanced stage lung cancer.Conclusion The observations indicate that more advanced studies and trials on C8092A SNPs are needed so as to assess if it could be used as a potential biomarker in the future.
文摘Objective:To study the correlation of ERCC1 and GSTP1 expression in esophageal cancer tissue with platinum-based chemotherapy sensitivity as well as apoptosis and proliferation gene expression.Methods: Patients with advanced esophageal cancer who accepted PF chemotherapy in our hospital between May 2013 and October 2015 were selected, esophageal cancer tissue was collected before the chemotherapy, the patients were divided into chemotherapy sensitivity group and chemotherapy resistance group according to the effect of chemotherapy, and the expression levels of ERCC1, GSTP1 as well as apoptosis and proliferation genes in esophageal cancer tissue were detected.Results:Protein content and positive protein expression rate of ERCC1 and GSTP1 in esophageal cancer tissue of chemotherapy sensitivity group were significantly lower than those of chemotherapy resistance group, MBP1, DEC1 and PTEN protein content were significantly higher than those of chemotherapy resistance group, and PLCE1, CyclinD1 and PAR2 protein content were significantly lower than those of chemotherapy resistance group;MBP1, DEC1 and PTEN protein content in esophageal cancer tissue with positive ERCC1 and GSTP1 expression were significantly lower than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression while PLCE1, CyclinD1 and PAR2 protein content were significantly higher than those in esophageal cancer tissue with negative ERCC1 and GSTP1 expression.Conclusion:The highly expressed ERCC1 and GSTP1 in esophageal cancer tissue can decreased the cancer cell sensitivity to platinum-based chemotherapeutics, inhibit cell apoptosis and promote cell proliferation during platinum-based chemotherapy.
