Gut microbiota changes associated with frailty in older adults:A systematic review of observational studies

BACKGROUND Frailty is a complex aging-related syndrome characterized by a cumulative loss of physiological reserve and increased vulnerability to adverse clinical outcomes,including falls,disability,incapacity and death.While an increasing number of studies suggest that the gut microbiota may play a key role in the pathophy-siology of frailty,direct evaluation of the association between gut microbiome alterations and frailty in older adults remains limited.AIM Seven electronic databases(China National Knowledge Infrastructure,VIP,SinoMed,Wanfang,PubMed,Web of Science and EMBASE)were searched for articles published before October 31,2023 to identify observational studies that compared the microbiomes of older adults with and without frailty.The diversity and composition of the gut microbiota were the main outcomes used to analyze the associations of changes in the gut microbiota with frailty in older adults.The quality of the included studies was assessed via the Newcastle-Ottawa Scale and the Agency for Healthcare Research and Quality.RESULTS Eleven observational studies with 912 older adults were included in this review.Consistent results revealed a significant difference in the gut microbiota composition between frail and non-frail older adults,with a significant decrease inαdiversity and a significant increase inβdiversity in frail older adults.The pooled results revealed that at the phylum level,four microbiota(Actinobacteria,Proteo-bacteria,Verrucomicrobia and Synergistetes)were significantly enriched,and two microbiota(Firmicutes and Fusobacteria)were significantly depleted in frail older adults.At the family level,the results consistently revealed that the abundances of 6 families,most of which belong to the Actinobacteria or Proteo-bacteria phylum,were greater in frail than in non-frail older adults.At the genus or species level,consistent results from more than two studies revealed that the abundances of the genera Prevotella,Faecalibacterium,and Roseburia were significantly lower in frail older adults;individual studies revealed that the abundances of some genera or species(e.g.,Megamonas,Blautia,and Megasphaera)were significantly lower,whereas those of other genera or species(e.g.,Bifidobacterium,Oscillospira,Ruminococcus and Pyramidobacter)were significantly greater in frail older adults.CONCLUSION This systematic review suggests that changes in the gut microbiota are associated with frailty in older adults,which is commonly reflected by a reduction in beneficial species and an increase in pathogenic species.However,further studies are needed to confirm these findings.

Fine Measurements and Analysis of Temperature Gradients in the Wells of the Jinsha River Groundwater Observational Network

Fine measurements have been conducted to temperatures and their gradients of six wells of the Jinsha River Groundwater Observational Network.The results show that the influence depths of sun radiation heat are 50m to 125m,average temperature gradients in the wells range from 0.11 to 2.81℃/hm and most are 1～2℃/hm,and the temperature gradients on varied depth sections of one well are highly changeable.Lithology of strata and their integrity,particularly high-angle crashed fault zones,have imposed major effects on the influence depths of sun radiation heat and temperature gradients of the wells.The micro dynamic characteristics of water temperature,such as coseismic effects,tidal effects and anomalies of the wells prior to earthquakes,probably depend,to a large degree,on the temperature gradients of the depths at which the water temperature sensors are settled.

Observational Facts Regarding the Joint Activities of the Southwest Vortex and Plateau Vortex after Its Departure from the Tibetan Plateau

Using atmospheric observational data from 1998 to 2013,station rainfall data,TRMM（Tropical Rainfall Measuring Mission） data,as well as annual statistics for the plateau vortex and shear line,the joint activity features of sustained departure plateau vortexes（SDPVs） and southwest vortexes（SWVs） are analyzed.Some new and useful observational facts and understanding are obtained about the joint activities of the two types of vortex.The results show that：（1） The joint active period of the two vortexes is from May to August,and mostly in June and July.（2） The SDPVs of the partnership mainly originate near Zaduo,while the SWVs come from Jiulong.（3） Most of the two vortexes move in almost the same direction,moving eastward together with the low trough.The SDPVs mainly act in the area to the north of the Yangtze River,while the SWVs are situated across the Yangtze River valley.（4） The joint activity of the two vortexes often produces sustained regional heavy rainfall to the south of the Yellow River,influencing wide areas of China,and even as far as the Korean Peninsula,Japan and Vietnam.（5） Most of the two vortexes are baroclinic or cold vortexes,and they both become strengthened in terms of their joint activity.（6） When the two vortexes move over the sea,their central pressure descends and their rainfall increases,especially for SWVs.（7） The two vortexes might spin over the same area simultaneously when there are tropical cyclones in the eastern and southern seas of China,or move southward together if a tropical cyclone appears near Hainan Island.

Endothelial progenitor cells, potential biomarkers for diagnosis and prognosis of ischemic stroke: protocol for an observational case-control study

Ischemic stroke is a devastating,life altering event which can severely reduce patient quality of life.Despite years of research there have been minimal therapeutic advances.Endothelial progenitor cells(EPCs),stem cells involved in both vasculogenesis and angiogenesis,may be a potential therapeutic target.After a stroke,EPCs migrate to the site of ischemic injury to repair cerebrovascular damage,and their numbers and functional capacity may determine patients'outcome.This study aims to determine whether the number of circulating EPCs and their functional aspects may be used as biomarkers to identify the type(cortical or lacunar)and/or severity of ischemic stroke.The study will also investigate if there are any differences in these characteristics between healthy volunteers over and under 65 years of age.100 stroke patients(50 lacunar and 50 cortical strokes)will be recruited in this prospective,observational case-controlled study.Blood samples will be taken from stroke patients at baseline(within 48 hours of stroke)and days 7,30 and90.EPCs will be counted with flow cytometry.The plasma levels of pro-and anti-angiogenic factors and inflammatory cytokines will also be determined.Outgrowth endothelial cells will be cultured to be used in tube formation,migration and proliferation functional assays.Primary outcome is disability or dependence on day 90 after stroke,assessed by the modified Rankin Scale.Secondary outcomes are changes in circulating EPC numbers and/or functional capacity between patient and healthy volunteers,between patient subgroups and between elderly and young healthy volunteers.Recruitment started in February 2017,167 participants have been recruited.Recruitment will end in November 2019.West Midlands-Coventry&Warwickshire Research Ethics Committee approved this study(REC number:16/WM/0304)on September8,2016.Protocol version:2.0.The Bayraktutan Dunhill Medical Trust EPC Study was registered in ClinicalTrials.gov(NCT02980354)on November 15,2016.This study will determine whether the number of EPCs can be used as a prognostic or diagnostic marker for ischemic strokes and is a step towards discovering if transplantation of EPCs may aid patient recovery.

Hepatitis B or C viral infection and risk of pancreatic cancer: A meta-analysis of observational studies

AIM: To investigate if there is an association between hepatitis B virus (HBV) or hepatitis C virus (HCV) infection and the risk of pancreatic cancer. METHODS: All relevant studies published before 11 October, 2012 were identified by a systematic search of MEDLINE, EMBASE, BIOSIS Previews and the Cochrane Library databases and with cross-referencing. The observational studies that reported RR or OR estimates with 95%CIs for the association between HBV or HCV and pancreatic cancer were included. A random-effects model was used to summarize meta-analytic estimates. The Newcastle-Ottawa quality assessment scale was applied to assess the quality of the methodology in the included studies. RESULTS: A total of 8 eligible studies were selected for meta-analysis. Overall, chronic hepatitis B and inactive hepatitis B surface antigen (HBsAg) carrier state (HBsAg positive) had a significantly increased risk of pancreatic cancer with OR of 1.20 (95%CI: 1.01-1.39), especially in the Chinese population (OR = 1.30, 95%CI: 1.05-1.56). Past exposure to HBV (possible occult HBV infection) had an increased OR of pancreatic cancer risk (OR = 1.24, 95%CI: 1.05-1.42), especially among those patients without natural immunity [anti hepatitis B core (HBc) positive/hepatitis B surface antibody (anti HBs) negative], with OR of 1.67 (95%CI: 1.13-2.22). However, past exposure to HBV with natural immunity (anti-HBc positive/anti-HBs positive) had no association with pancreatic cancer development, with OR 0.98 (95%CI: 0.80-1.16), nor did the HBV active replication (hepatitis B e antigen positive status), with OR 0.98 (95%CI: 0.27-1.68). The risk of pancreatic cancer among anti-HBs positive patients was significantly lower than among anti-HBs negative patients (OR = 0.54, 95%CI: 0.46-0.62). Past exposure to HCV also resulted in an increased risk of pancreatic cancer (OR = 1.26, 95%CI: 1.03-1.50). Significant between-study heterogeneity was observed. Evidence of publication bias for HBV/HCV infection-pancreatic cancer association was not found. CONCLUSION: Chronic HBV and HCV infection increases pancreatic cancer risk. Our findings underscore the need for more studies to confirm this potential relationship.

An Observational Study of the 30-50 Day Atmospheric Oscillations Part I: Structure and Propagation

Features of structure and propagation of the 30 to SO day atmospheric oscillations are investigated using the ECMWF analysis of 1980-1983. Evidence is provided to confirm the characteristics of the oscillation in the equatorial region. Those in the mid-high latitudes, however, are revealed to be very different from the tropics and pose a strong barotropic structure. Horizontal coherence shows teleconnection patterns which can be identified as EAP and PNA. The wind field of the specified time scale of the oscillation appears as long-lived vortices and vortex pairs. Mid-latitude perturbations propagate clearly westwards, especially during the winter season. In the high latitudes, they propagate westwards in the winter but eastwards in the summer. Meridional propagations are rather different from region to region.

Observational Study on the Onset of the South China Sea Southwest Monsoon

Based on the long-term marine ship observation data, records of meteorological stations and High-Reflective Cloud(HRC) data by satellite remote sensing , this paper has studied the circulation patterns and variability in elements during onset and the established periods of the South China Sea(SCS) southwest(SW) monsoon. The averaged date of the onset SW monsoon in the SCS occurs in the middle of May climatologically. The corresponding date for the northern part is little earlier (May 12) and those for the southern parts are little later (May 20). The interannual range of the onset dates is about one month. Following the onset of the SW monsoon, the cloud amount and the precipitation increase while the convection activities enhance over the SCS. But there is a strong spatial heterogeneity within the domain. After onset of the SW monsoon the strong convective area moves northwards, while the SCS rain band moves to the center and north. Sea surface temperature(SST) increases rapidly before the onset and the leading time is about one month. The increment of SST supplies heat and vapor for the onset. From April to May the surface heat fluxes display obvious changes, e.g., latent heat exchange and evaporation enhancement. It is one of the reasons why the SW monsoon bursts firstly in the SCS.

Outcomes of different minimally invasive surgical treatments for vertebral compression fractures:An observational study

BACKGROUND Osteoporosis with vertebral compression fractures is increasingly common in the elderly population.Cement augmentation is one of the effective surgical treatments for these patients.Currently,there are several different types of cement augmentation treatments.No studies have compared the safety and efficacy of different cement augmentation types for the treatment of such fractures;thus,we retrospectively compared vertebroplasty,balloon kyphoplasty,and kyphoplasty with SpineJack or an intravertebral expandable pillar.AIM To compare the postoperative safety and efficacy of each surgical intervention in treating vertebral compression fractures.METHODS We retrospectively analyzed 354 patients with acute vertebral compression fractures,defined as signal changes in the T1 weighted magnetic resonance imaging,and randomly divided the patients into five groups.Their visual analog scale scores for pain,kyphotic angle,average body height,rate of cement leakage,and occurrence of adjacent vertebral compression fractures were followed for 1 year.One-way analysis of variance,the post hoc Bonferroni test,and Fisher exact probability test were used for statistical analyses.RESULTS All pain scores significantly improved 12 mo postoperatively;however,there was no significant difference between the groups(P=0.325).Kyphoplasty with SpineJack significantly reduced the kyphotic angle(P=0.028)and restored the height of the vertebral body(P=0.02).The rate of adjacent compression fractures was the highest in the vertebroplasty group,with a statistically significant difference according to the Fisher exact probability test(P=0.02).The treatment with the lowest cement leakage rate cannot be identified because of the small sample size;however,kyphoplasty with SpineJack,an IVEP,and vesselplasty resulted in lower rates of cement leakage than balloon kyphoplasty and vertebroplasty.CONCLUSION Kyphoplasty with SpineJack has good outcomes in kyphotic angle reduction and body height restoration.Vertebroplasty has the highest cement leakage rate and adjacent compression fracture occurrence.

An Observational Study of the 30-50 Day Atmospheric Oscillations Part II: Temporal Evolution and Hemispheric Interaction across the Equator

In this part, the temporal evolution and interaction across the equator of 30-50 day oscillation in the atmosphere are investigated further. The annual variation of 30-50 day oscillation is quite obvious in the mid-high latitudes. In the tropical atmosphere, the obvious interannual variation is an important property for temporal evolution of 30-50 day oscillation. The low-frequency wavetrain across the equator over the central Pacific and central Atlantic area, the movement of the long-lived low-frequency system across the equator and the meridional wind component across the equator will obviously show the interaction of 30-50 day oscillation in the atmosphere across the equator.

Red meat intake and the risk of endometrial cancer:Metaanalysis of observational studies

AIM:To evaluate whether red meat intake is related to the risk of endometrial cancer(EC) using meta-analysis.METHODS:We searched Pub Med,EMBASE,and the Cochrane Library up to June 2013,using common keywords related to red meat and EC.Case-control studies and cohort studies comparing the risk of endometrial cancer among categories by the amount of intake were included.Eleven case-control studies and five cohort studies met our criteria.We performed a conventional and a dose-response meta-analysis of case-control studies using the Der Simonian-Laird method for random-effects.For cohort studies we performed a conventional meta-analysis.Publication bias was evaluated using Egger's test.RESULTS:In the meta-analysis of 11 case-control studies including 5419 cases and 12654 controls,higher red meat consumption was associated with an increased risk of EC [summary relative risk(SRR) = 1.43,95%CI:1.15-1.79;I2 = 73.3% comparing extreme intake categories).In a dose-response analysis,for red meat intake of 100 g/d,SRR was 1.84(95%CI:1.64-2.05).In contrast,in the meta-analysis of five prospective studies including a total of 2549 cases among 247746 participants,no significant association between red meat intake and EC risk(SRR = 0.97,95%CI:0.85-1.11;I2 = 4.9% comparing extreme intake categories) was observed.CONCLUSION:Our meta-analysis found a significantlinear association between red meat intake and EC risk based on case-control studies but this was not confirmed in prospective studies.

Observational results of MUSER during 2014–2019

The solar radio signal that can be received by the ground-based telescopes covers a wide frequency range,allowing us to monitor the complex physical processes occurred from the solar surface to the vast interplanetary space.MingantU SpEctral Radioheliograph(MUSER),as the latest generation of solar dedicated radio spectral-imaging instrument in the centimeter-decimeter wavelengths,has accumulated a large number of observational data since its commissioning observation in 2014.This paper presents the main observational results identified by MUSER from 2014 to 2019,including the quiet Sun and 94 solar radio burst events.We find that there are 81 events accompanied with Geostationary Operational Environmental Satellites(GOES)soft X-ray(SXR)flares,among which the smallest flare class is B1.0.There are 13 events without accompanying any recorded flares,among which the smallest SXR intensity during the radio burst period is equivalent to level-A.The main characteristics of all radio burst events are presented,which shows the powerful ability of MUSER to capture the valuable information of the solar non-thermal processes and the importance for space weather.This work also provides a database for further in-depth research.

Wind Speed and Altitude Dependent AMDAR Observational Error and Its Impacts on Data Assimilation and Forecasting

Aircraft Meteorological Data Relay(AMDAR)observations have been widely used in numerical weather prediction(NWP)because of its high spatiotemporal resolution.The observational error of AMDAR is influenced by aircraft flight altitude and atmospheric condition.In this study,the wind speed and altitude dependent observational error of AMDAR is estimated.The statistical results show that the temperature and the observational error in wind speeds slightly decrease as altitude increases,and the observational error in wind speed increases as wind speed increases.Pseudo single AMDAR observation assimilation tests demonstrate that the wind speed and altitude dependent observational error can provide more reasonable analysis increment.Furthermore,to assess the performance of wind speed and altitude dependent observational error on data assimilation and forecasting,two-month 3-hourly cycling data assimilation and forecast experiments based on the Weather Research and Forecasting Model(WRF)and its Data Assimilation system(WRFDA)are performed for the period during 1 September-31 October,2017.The results of the two-month 3-hourly cycling experiments indicate that new observational error improves analysis and forecast of wind field and geo-potential height,and has slight improvements on temperature.The Fractions Skill Score(FSS)of the 6-h accumulated precipitation shows that new wind speed and altitude dependent observational error leads to better precipitation forecast skill than the default observational error in the WRFDA does.

A Comparative Observational Study of the Use Transvaginal Ultrasound and Hysteroscopy for the Detection of Uterine Cavity Pathologies in Women with Abnormal Uterine Bleeding

Background: 70% of all gynecological consultations are for abnormal uterine bleeding. Any approach to optimal management begins with an appropriate diagnosis. 40% of premenopausal women with abnormal uterine bleeding were found to have some intrauterine pathology. This study aims to compare the diagnostic value of transvaginal ultrasonography in comparison to hysteroscopy in detecting uterine abnormalities in patients with abnormal uterine bleeding. Methods: Retrospective observational cross sectional study of 250 women presented with abnormal uterine bleeding. The patients who fulfilled the selection criteria and have been sequentially investigated by transvaginal ultrasound (TVS) and hysteroscopy were included. Results: 90% of patients were from 35 - 49 yrs. 81.2% of patients have body mass index above 25 kg/m2. Transvaginal ultrasound (TVS) compared well with high sensitivity as regards normal endometrium. (TVS) missed 4 patients of endometrial polyps and one patient of sub mucous fibroid. Three patients of adenomyosis were only diagnosed by (TVS);they were reported as being normal by hysteroscopy. Conclusion: (TVS) is considered as an excellent approach to the initial evaluation of uterine pathologies in patients with abnormal uterine bleeding.

A Comprehensive Observational Analysis for the Effects of Gas Cannons on Clouds and Precipitation

To analyze the effects of gas cannons on clouds and precipitation,multisource observational data,including those from National Centers for Environmental Prediction(NCEP)reanalysis,Hangzhou and Huzhou new-generation weather radars,laser disdrometer,ground-based automatic weather station,wind profiler radar,and Lin'an C-band dualpolarization radar,were adopted in this study.Based on the variational dual-Doppler wind retrieval method and the polarimetric variables obtained by the dual-polarization radar,we analyzed the microphysical processes and the variations in the macro-and microphysical quantities in clouds from the perspective of the synoptic background before precipitation enhancement,the polarization echo characteristics before,during and after enhancement,and the evolution of the fine three-dimensional kinematic structure and the microphysical structure.The results show that the precipitation enhancement operation promoted the development of radar echoes and prolonged their duration,and both the horizontal and vertical wind speeds increased.The dual-polarization radar echo showed that the diameter of the precipitation particles increased,and the concentration of raindrops increased after precipitation enhancement.The raindrops were lifted to a height corresponding to 0 to-20℃due to vertical updrafts.Based on the disdrometer data during precipitation enhancement,the concentration of small raindrops(lgN_(w))showed a significant increase,and the mass-weighted diameter D_(m)value decreased,indicating that the precipitation enhancement operation played a certain“lubricating”effect.After the precipitation enhancement,the concentration of raindrops did not change much compared with that during the enhancement process,while the Dm increased,corresponding to an increase in rain intensity.The results suggest the positive effect of gas cannons on precipitation enhancement.

An overview on observational instruments and measuring methods for dewfall

This paper systematically summarizes previous measuring methods and observational instruments for the magnitude of dewfall on land surface, analyzes the characteristics of common observational instruments for land surface dewfall, and describes several basic dewfall measurement methods. Moreover, the basic principles of these methods and instruments are interpreted, and their advantages, disadvantages, and applicability are analyzed. Recommendations for the further improvement of these observational instruments and the development of dewfall measuring methods are presented, and new technologies and scientific proposals for exploiting dewfall are elucidated.

Predictors of Discontinuation of Antipsychotic Therapy in Patients with Acute Schizophrenia: A 1-Year Observational Study with More Than 1000 Patients

Discontinuation of antipsychotic therapy has been a significant clinical issue among patients with schizophrenia, since the patients who discontinued antipsychotic treatment showed worse clinical and functional outcomes, and higher risks of relapse of schizophrenia symptoms and hospitalization. We conducted a post-hoc analysis of a post-marketing research with a 12-month follow-up period to identify the predictors for discontinuation of antipsychotic monotherapy in Japan. This is a prospective, naturalistic multicenter observational study, designed to evaluate the discontinuation rates of olanzapine monotherapy and non-olanzapine antipsychotic monotherapy in Japanese adult patients with acute schizophrenia. Patients were treatment-naive, or had switched from other antipsychotics or from poly-pharmacotherapy to oral antipsychotic monotherapy. We analyzed the correlation of discontinuation of antipsychotic monotherapy with baseline characteristics of patients. A total of 1089 patients (578 patients treated with olanzapine and 511 with non-olanzapine antipsychotics) were eligible for analysis. By the end of the 12-month study period, 614 patients (56.4%) discontinued antipsychotic therapy. Multivariate logistic regression analyses indicated significantly lower discontinuation rates in all patients treated with antipsychotics: older age (Odds ratio [OR], 0.871;95% confidence interval [CI], 0.797 to 0.953;p = 0.003), outpatient status (OR, 0.508;95% CI, 0.383 to 0.675;p < 0.001), prior use of antipsychotics (OR, 0.693;95% CI, 0.516 to 0.930;p = 0.015), and olanzapine group showed lower discontinuation rate than that of non-olanzapine group (OR, 1.416;95% CI, 1.086 to 1.846;p = 0.010). The present study indicated that the outpatient status, older age, and prior use of antipsychotics have better adherence to antipsychotic treatment. In addition to these factors, use of anti-parkinson agents showed lower discontinuation rates in the olanzapine monotherapy group.

Educational Practices in the Model of Music Learning Theory of E. Edwin Gordon： An Observational Research

This paper analyzes the supervision activity, to which educators and teachers enrolled with AIGAM （Gordon Italian Association for the Musical Learning） are subject to every year and intends to verify the application of those principles expressed in the learning model of the MLT （Music Learning Theory） developed by educational psychologist E. Edwin Gordon （1989, 1999, 2000, 2001, 2007） and promoted internationally by various institutions and organizations specifically accredited. It describes the influence of the videotaped supervision on the process, functions of monitoring, and evaluation of educational practices, starting with an empirical model that has guided the interventions in a study of supervision on training aimed at consolidating and developing professional skills in music education in early childhood. This paper sought to understand： the kind of practices, interactions, communications developing during an educational actions, the existence of a consistent relationship between the principles expressed in the MLT and their application, the type and benefits of supervision performed by of video recording on stakeholders in terms of change in professional behavior, and finally whether the active supervision could be comparable with other kinds of approaches.

Determination of Causal Effect in Observational Studies： Analysis of Correlated Data with Binary End-Points

Identifying the causal impact of＇ some intervention challenging when one is faced with correlated binary end-points in observational studies is a challenging task, and it is even more The statistical literature on analyzing such data is well documented. Dependence between observations from the same study subject in correlated data renders invalid the usual chi-square tests of independence and inflates the variance ofparameter estimates. Disaggregated approaches such as hierarchical linear models which are able to adjust for individual level covariate：s are favoured in the analysis of such data, thereby gaining power over aggregated and individual-level analyses. In this article the authors, therefore, address the issue of analyzing correlated data with dichotomous end-points by using hierarchical logistic regression, a generalization of the standard logistic regression model for independent outcomes.

Observational characteristics and dynamic mechanism of low-salinity water lens for the offshore detachment of the Changjiang River diluted water in August 2006

The Changjiang River diluted water(CDW)spreads into the East China Sea(ECS)primarily in a plume pattern,although in some years,low-salinity water lenses(LSWLs)detach from the main body of the CDW.In-situ observations indicate that in August 2006,a LSWL detached from the main body of the CDW near the river mouth.In this paper,the effects of winds,tides,baroclinity and upwelling on LSWLs are explored with a threedimensional model.The results show that:(1)winds play a crucial role in these detachment events because windinduced northerly Eulerian residual currents impose an uneven force on the CDW and cut it off,thus forming a LSWL;(2)upwelling carries high-salinity water from the lower layer to the upper layer,truncating the low-salinity water tongue vertically,which is conducive to the detachment and maintenance of LSWLs;and(3)upwelling during the evolution of a LSWL is caused by the combined effects of winds and tides.The influences of windinduced upwelling are mainly near the shore,whereas the upwelling along the 30 m isobath is predominantly affected by tides,with the effect increasing from neap tide to spring tide.

The research objectives and observational possibilities for fast moving near-Earth asteroids

The paper describes observations of fast-moving near-Earth asteroids(NEAs) made with the small ground-based telescopes of National Time Service Center of Chinese Academy of Science(NTSC of CAS) and Research Institute "Mykolaiv Astronomical Observatory"(RI MAO) by the rotating-drift-scan CCD(RDS CCD) technique. This technique is used to obtain the point images of both the studied objects and reference stars. The results of ongoing follow-up observations of NEAs are discussed. The residual differences(O-C) between the observed and calculated positions from JPL ephemeris were generally small for these asteroids. The standard deviations of these differences were typically ±(0.2′′-0.3′′) in both coordinates for objects with apparent velocity which substantially exceed FWHM for the given exposure time. The results of comparative statistics for such observations from the MPC database show that this is a good level of precision for NEAs. Moreover, the telescopes with the RDS CCD technique implemented can observe the NEAs that closely approach the Earth and with enough observations can improve the precision of determining their orbital elements and impact predictions.