Pomegranate （Punica granatum L.） Peel Extract as a Source of Natural Antioxidant

An increase in the demand for natural additives has shifted the attention from synthetic to natural antioxidants. Antioxidative components from pomegranate peel were extracted by using different solvents like methanol, ethanol, acetone, chloroform and ethyl acetate. All the extracts were tested for Total phenolic content （TPC） and the methanol which proved to be best in extracting the phenolic compounds was used to assess the antioxidant property by using different methods like Diphenyl piridyl picryl hydrazyl （DPPH）, Relative reducing power （RRP）, Beta carotene bleaching and Ferric thiocyanate （FTC）. The antioxidant activity （AA） of pomegranate peel extract （PPE） was compared with Butylated hydroxyl toluene （BHT） and Butylated hydroxyl anisole （BHA）. The methanolic extract was examined by Liquid Chromatography and Mass Spectroscopy （LCMS） and the spectra showed the presence of different phenolic compounds. The extracts were tested for their efficiency in preventing rancidity of Refined Bleached Deodorized Soybean oil （RBDSBO） in comparison with BHT and control at 200 ppm concentration. The PPE proved to be best in preventing rancidity. Owing to the present results, the studies can further be extended to exploit the PPE for application into different food products.

Chemical Composition,Anticancer,Anti-neuroinflammatory,and Antioxidant Activities of the Essential Oil of Patrinia scabiosaefolia

Objective：To study the chemical composition,anticancer,anti-neuroinflammatory,and antioxidant activities of the essential oil of Patrinia scabiosaefolia（EO-PS）.Methods：Patrinia scabiosaefolia was analyzed by gas chromatography-mass spectrometry.Eight human carcinoma cell lines,including SGC-7901,AGS,Hep G2,HT-29,HCT-8,5-FU/HCT-8,He La,and MDA-MB-231,were assessed by methylthiazolyldiphenyltetrazolium bromide（MTT） assay.Anti-neuroinflammatory activity was assessed by production of interleukin（IL）-1β and IL-6 induced by lipopolysaccharide in BV-2 cells（microglia from mice）.The antioxidant activity was evaluated with a 2,2-diphenyl-1-picrylhydrazyl（DPPH） scavenging assay.Results：Forty-four components,representing 83.919% of the total oil,were identified in the EO-PS.The major constituents were caryophyllene oxide（12.802%）,caryophyllene（6.909%）,α-caryophyllene（2.927%）,β-damascenone（3.435%）,calarene（5.621%）,and phenol（3.044%）.The MTT assay showed that the EO-PS exhibited significant dose-dependent growth inhibition in the 50–200 μg/m L dilution range.The EO-PS exhibited a dose-dependent scavenging activity against the DPPH radical,with an half of maximal inhibitory concentration 1.455 mg/m L.Conclusion：The EO-PS possesses a wide range of antitumor,anti-neuroinflammatory and antioxidant activities,suggesting that it may be a good candidate for further investigations of new bioactive substances.