Multiple biostimulation treatments were applied to enhance the removal of heavy crude oil pollutants in the saline soil of Yellow River Delta.Changes of the soil bacterial community were monitored using the terminal r...Multiple biostimulation treatments were applied to enhance the removal of heavy crude oil pollutants in the saline soil of Yellow River Delta.Changes of the soil bacterial community were monitored using the terminal restriction fragment length polymorphism （T-RFLP） and clone library analyses.The 140-day microcosm experiments showed that low C：N：P ratio,high availability of surfactant and addition of bulking agent significantly enhanced the performance,leading to the highest total petroleum hydrocarbon removal.Meanwhile,the bacterial community was remarkably changed by the multiple biostimulation treatments,with the Deltaproteobacteria,Firmicutes,Actinobacteria,Acidobacteria and Planctomycetes being inhibited and the Alphaand Beta-proteobacteria and some unknown Gammaproteobacteria bacteria being enriched.In addition,different hydrocarbon-degraders came to power in the following turn.At the first stage,the Alcanivorax-related Gammaproteobacteria bacteria dominated in the biostimulated soil and contributed mainly to the biodegradation of easily degradable portion of the heavy crude oil.Then the bacteria belonging to Alphaproteobacteria,followed by bacteria belonging to Candidate division OD1,became the dominant oil-degraders to degrade the remaining recalcitrant constituents of the heavy crude oil.展开更多
Oil production related activities have led to many environmental problems.Around 80%of the total output of crude oil is generated from terrestrial oilfields in the world.However,the impact of oil exploitation procedur...Oil production related activities have led to many environmental problems.Around 80%of the total output of crude oil is generated from terrestrial oilfields in the world.However,the impact of oil exploitation procedures on soil animal communities has not been fully understood.This study investigated the responses of soil nematode communities to the oil exploitation activities in the Yellow River Delta of China.By setting 10 oilfield sites and 5 relatively uncontaminated sites(controls),we found that the content of soil total petroleum hydrocarbons(TPH)was significantly higher at oilfield sites than at controls.With a longer oil exploitation history,the content of soil TPH increased.Soil nematode community structure at oilfield sites was largely different from that at controls.Soil nematodes were significantly less abundant but more diverse at oilfield sites than at controls.The proportions of fungal feeders were significantly lower at oilfield sites than at controls,attaining only half of those at controls.The nematode trophic diversity and genus number negatively correlated with the duration of petroleum exploitation history.This study elucidated the difference in soil nematode communities caused by oilfield exploitation and indicated that the nematode diversity was most obviously influenced by the soil TPH content and the oil exploitation history.展开更多
基金supported by the International Cooperation Project of National Natural Science Foundation of China (No.40821140541)the National High Technology Research and Development Program (863) of China (No.2009AA063501)
文摘Multiple biostimulation treatments were applied to enhance the removal of heavy crude oil pollutants in the saline soil of Yellow River Delta.Changes of the soil bacterial community were monitored using the terminal restriction fragment length polymorphism （T-RFLP） and clone library analyses.The 140-day microcosm experiments showed that low C：N：P ratio,high availability of surfactant and addition of bulking agent significantly enhanced the performance,leading to the highest total petroleum hydrocarbon removal.Meanwhile,the bacterial community was remarkably changed by the multiple biostimulation treatments,with the Deltaproteobacteria,Firmicutes,Actinobacteria,Acidobacteria and Planctomycetes being inhibited and the Alphaand Beta-proteobacteria and some unknown Gammaproteobacteria bacteria being enriched.In addition,different hydrocarbon-degraders came to power in the following turn.At the first stage,the Alcanivorax-related Gammaproteobacteria bacteria dominated in the biostimulated soil and contributed mainly to the biodegradation of easily degradable portion of the heavy crude oil.Then the bacteria belonging to Alphaproteobacteria,followed by bacteria belonging to Candidate division OD1,became the dominant oil-degraders to degrade the remaining recalcitrant constituents of the heavy crude oil.
基金supported by the National Basic Research Program of China(No.2006CB403305).
文摘Oil production related activities have led to many environmental problems.Around 80%of the total output of crude oil is generated from terrestrial oilfields in the world.However,the impact of oil exploitation procedures on soil animal communities has not been fully understood.This study investigated the responses of soil nematode communities to the oil exploitation activities in the Yellow River Delta of China.By setting 10 oilfield sites and 5 relatively uncontaminated sites(controls),we found that the content of soil total petroleum hydrocarbons(TPH)was significantly higher at oilfield sites than at controls.With a longer oil exploitation history,the content of soil TPH increased.Soil nematode community structure at oilfield sites was largely different from that at controls.Soil nematodes were significantly less abundant but more diverse at oilfield sites than at controls.The proportions of fungal feeders were significantly lower at oilfield sites than at controls,attaining only half of those at controls.The nematode trophic diversity and genus number negatively correlated with the duration of petroleum exploitation history.This study elucidated the difference in soil nematode communities caused by oilfield exploitation and indicated that the nematode diversity was most obviously influenced by the soil TPH content and the oil exploitation history.
