GC-MS Analysis of Volatile Oils in Mango Leaves from Different Producing Areas

[Objectives]To analyze the composition of volatile oils from mango leaves from different producing areas.[Methods]The volatile oils in mango leaves from different areas was extracted by steam distillation,and the GC-MS method combined with retention index and peak area normalization method was used for qualitative and quantitative analysis.[Results]The volatile oils of mango leaves in southern provinces contained high content of terpenes,such asα-gurjunene(18.39%-34.13%),(+)-viridiflorene(8.95%-16.30%)and(E)-β-ocimene(3.49%-7.63%).The volatile oils components of mango leaves were significantly different in different provinces,for example,mango leaves produced in Hainan Province contained a large amount ofβ-selinene(23.00%),but not in other provinces.[Conclusions]The origin had a great influence on the composition and contents of volatile oils in mango leaves.

Identification and mechanism of formation of certain mutagenic components in the volatile condensates from oxidized and heated edible oils

Edible oils-rapeseed oil, soybean oil and peanut oil are heated in air at 270-280 ℃respectively. The volatile condensates are collected and analyzed by means of GC/MS. Eithercrotonaldehyde or 2-methylacrolein is found respectively in the condensates of rapeseed oil andsoybean oil. Neither crotonaldehyde nor 2-methylacrolein is found in the condensates of peanut oil.Mechanism of formation of crotonaldehyde is discussed.

Effects of Volatile Oils of Sichuan Citrisarcodactylis fructus on Proliferation and Apoptosis of MCF-7 Human Breast Cancer Cells

[Objectives] To study the effects of volatile oils of Sichuan Citrisarcodactylis fructus on proliferation and apoptosis of MCF-7human breast cancer cells.[Methods]CCK-8 method was used to measure the cell proliferation,the flow cytometry was used to measure changes in cell cycle,Western blot was used to detect p53 and cyclin D1 activity changes,TUNEL method was used to measure percentage of apoptotic cells,inverted phase contrast microscope and transmission electron microscope were used to observe morphology of MCF-7 cells treated with different concentrations.[Results]When the concentration was 5-50 μmol/L,the cell proliferation inhibition rate increased significantly(P <0. 05),G2/M phase decreased significantly( P < 0. 05),eventually disappeared completely,G1 phase significantly increased with time and concentration( P < 0. 05),finally reached 90. 0%; the activity of cyclin D1 significantly declined( P < 0. 05),while the activity of p53 had no significant change( P > 0. 05). The apoptotic rate of MCF-7 cells significantly increased with the extension of time( P < 0. 05). At6 h,12 h and 24 h of action time,the apoptotic rate of MCF-7 cells significantly increased with the increase in volatile oil concentration(P <0. 05). Morphological observation showed that the apoptosis of MCF-7 cells was obvious.[Conclusions]The volatile oils of Citrisarcodactylis fructus have obvious inhibition of proliferation of MCF-7 cells and function of inducing apoptosis,and the effects took on the dose and time dependent. 5-50 μmol/L volatile oil of Sichuan Citrisarcodactylis fructus can inhibit the proliferation of MCF-7 cells and induce the apoptosis of MCF-7 cells through inhibiting the cyclin D1.

Investigation of the volatile oils,lipid constituents and biological activity of Ballota andreuzziana,Teucrium zanonii and Verbena tenuisecta in Libya

Objective:To determine the chemical composition of essential oils and lipid constituents of Ballola andreuzziana(B.andreuzziana),Teucrium zanonii(T.zanonii) and Verbena tenuisecta(V. tenuisecta) growing in Libya,and to test the antibacterial activity of different extracts of Teucrium zanonii.Methods:The volatile oils of all plants were extracted by hydrodistillation method and analyzed by GC/MS method.The lipid constituents of plants were obtained by extraction with petroleum ether and fractionated into fatty alcohols,fatty acids and unsapoinfiable matters. Antibacterial activity of T.zanonii extracts and antioxidant activity of different extracts of T. zanonii were also studied.Results:The volate oil of B.andreuzziana was found to consists of 20 compounds in which caryophyllin is the main one(63.1%),the volatile oil of T.zanonii consists of 74 compounds in which germacrene-D was the main compound,while the volatile oil of V. tenuisecta consists of 13 compounds with l-octen-3- ol as a major constituent(52.87%).The study of antimicrobial activity of different extracts of V.tenuisecta showed that,both methanol and butanol extracts exhibited the highest activity against Mycobacterium phlei(M.pblei) and Candida albicans(C.albicans) respectively,while petroleum ether,fatty alcohols and unsaponifiable fractions had no antimicrobial activity against all the tested microorganisms. Investigation of the antioxidant activity of different extracts of T.zanonii using DPPH method proved that,the ethyl acetate and butanol fractions showed the highest activity where the inhibition percentage(1%) are 93.6 and 92.1 respectively.Conclusions:This is the first report about the volatile oils of these plants where T.zanonii have the highest content and the highest number of the identified compounds.The study of antioxidant T.zanonii extracts proved that,the ethyl acetate,butanol and aqueous extracts have the highest antioxidant activity.Methanol and butanol extracts of V.tenuisecta exhibited the highest activity against M.phlei and C.albicans respectively.

Physicochemical properties and volatile components of hempseed oils in Bama region

A comparison study has been performed in hempseed oils (HSO) extracted from different cultivars in Bama region. Squeezing properties, chemical compositions, thermal stability and flavor profile of pressed HSO were determined in this study. Results indicated that there were no significant differences in crude fat, protein, fiber of different hempseeds and fatty acid composition in HSOs. Hempseeds with small size and dark-colored peel have lower squeezing oil yield than big ones. Average content of total phytosterol, tocopherol and phenol in HSO from small seeds were 872.0 mg/100 g, 124.1 mg/100 g and 15.5 mg SA/100 g respectively. Thermal oxidation by differential scanning calorimetry (DSC) revealed three step oxidation of HSO with mean onset and oxidation temperature at 124oC and 135-315oC. Oxidation temperature of HSO from small seeds was higher than that from big seeds (big size and light-colored peel). Main volatile ingredients in HSO contained 11 kinds of monoterpene and 7 kinds of sesquiterpene. Volatile terpene content in HSO from small seeds was nearly 30% higher than that of big seeds. Quality of HSO with different peel feature has significant difference in squeezing yield, thermal stability and volatile flavors. This work may be useful to produce high quality hempseed oils through quality evaluation.

Rosa x alba:source of essential minerals and volatile oils

Rosa x alba is cultivated almost exclusively for the production of aromatic water and fruits widely used as ingredients in some traditional food preparations sold commercially.Results of the proximate and elemental analyses revealed that flowers exhibited higher moisture content while the dry matter content was higher in leaves.Total fat and ash was higher in fruits when compared with leaves and flowers.All studied organs were found to be rich in essential mineral such as K,Ca,P and Mg.Qualitative and quantitative analysis of the volatile oils revealed different composition patterns between organs.Linalool and geraniol were found as major constituents of the leaf oil whereas 2-phenethyl alcohol and eugenol were the major constituents of the floral oil.In contrast,fruits showed very distinct composition and alkanes/alkenes were found to have the major contribution.The present composition could justify the traditional use of Rosa x alba which could be considered as a potential source of essential minerals and volatile constituents.

GC-MS Analysis of Chemical Constituents of Volatile Oils of the Yao Medicine Thunbergia grandiflora from Different Habitats in Guangxi

[Objectives]This study was conducted to compare and analyze the chemical constituents of the volatile oils of the Yao medicine Thunbergia grandiflora from different habitats.[Methods]A quartz capillary column DB-1MS,an EI ion source and a quadrupole mass analyzer were used for analysis.The chromatographic and mass spectrum information obtained was automatically retrieved and analyzed by data processing system and its memory spectrum library(Nist.08).The relative content of each chemical component in the volatile oil was determined by the peak area normalization method.[Results]Forty nine chromatographic peaks were isolated from the sample produced in Shitun,Bailongtan Town,Mashan County,and 24 chemical constituents were identified,accounting for 88.78% of the total volatile oil.Forty nine chromatographic peaks were isolated from the sample produced in Hongdu Village,Chengjiang Town,Duan County,and 30 chemical constituents were identified,accounting for 88.38% of the total volatile oil.Forty eight chromatographic peaks were isolated from the sample produced in Longwan Township,Du'an County,and 25 chemical constituents were identified,accounting for 80.01% of the total volatile oil.Nine chemical constituents were common to the volatile oils of the samples from the three habitats.[Conclusions]It could be seen that the main components of volatile oils from T.grandiflora produced in different areas are different.

Exploration of the efficacy and mechanism of treating head wind disease with the combination change of ginger volatile oil and gingerol by using content-weighted network pharmacology technology

Background:Exploring the efficacy,potential components,and mechanism of the combination of ginger essential oil and gingerols in the treatment of head wind disease based on network pharmacology technology with content weight.Methods:The experimental groups were divided into:0:10,1:4,1:2,1:1,2:1,4:1,10:0.The relative content(Ri)of the chemical constituents of ginger's volatile oil was determined using gas chromatography-mass spectrometry(GC-MS).Additionally,the physicochemical and biological property parameters(LogP,MDCK,PPB,MW)of the components were considered.To assess the quantitative effect of the components,a grading score was performed,and the quantitative effect index(Ki)was calculated.Subsequently,the target effect index(Ti)was calculated by combining the component-target matching score(Fit score).Using these calculations,the target effect score A was determined under the influence of multiple components targeting different targets.Key targets with A≥1000 were identified.To predict the targets related to head wind disease,the Comparative Toxicogenomics Database(https://ctdbase.org/),Gene Cards(https://www.genecards.org/),and Disgenet database(https://www.disgenet.org/)were utilized.The key targets,obtained from different proportions of ginger's volatile oil and gingerol,were intersected with the predicted targets.This facilitated network pharmacological analysis and verification of the efficacy.Results:The content of volatile oil in ginger demonstrated an impact on key targets associated with the volatile oil group.Each specific combination of volatile oil consistently activated distinct pathways,with variations stemming from changes in content.Experimental testing revealed that different combinations of ginger's volatile oil and gingerol effectively alleviated migraine symptoms in rats.Conclusion:Through the application of content-weighted network pharmacology technology and pharmacodynamic verification,it was determined that altering the ratio between ginger's volatile oil and gingerol leads to variations in potential targets and pathways,consequently impacting its efficacy.

Volatilization behaviors of diesel oil from the soils

The volatilization of diesel oil, Shengli crude oil and 90# gasoline on glass surface of petri dishes were conducted at the ambient temperature of 25℃. Diesel oil evaporates in a power manner, where the loss of mass is approximately power with time. 90# gasoline evaporates in a logarithmic with time. Where as the volatilization of Shengli crude oil fit either the logarithmic or power equation after different time, and has similar R2. And the effects of soil type and diesel oil and water content on volatilization behavior in unsaturated soil were studied in this paper. Diesel oil and water content in the soils play a large role in volatilization from soils. Appropriate water helps the wicking action but too much water stops it. The wicking action behaves differently in four different types of soils in the same volatilization experiment of 18% diesel oil content and air-dry condition.

The Use of TEOST MHT-4 to Measure the Phosphorus Volatility of Engine Oils

Phosphorus is known to poison the exhaust gas catalysts used to control emissions in modern gasoline-fueled passenger car vehicles.Phosphorus volatility has been studied by others as a potential issue related to the compatibility of modern engine oils with exhaust gas catalysts using a modified version of the ASTM D5800(Noack) test as well as the Sequence ⅢG engine test(ASTM D7320).The level of phosphorus volatility in these tests has been shown to be strongly affected by the chemistry of the zinc dialkyldithiophosphate(ZDDP) used in the engine oil formulation.The TEOST MHT-4 test(ASTM D7097) is a high temperature deposit test currently included in the ILSAC GF-4 specification.This bench test simulates the oxidation and deposit-forming characteristics of engine oils in the hot zones of modern high-performance engines and,therefore,was chosen by the author(s) as a tool to investigate differences in phosphorus volatility of modern engine oils.Our investigations demonstrate that the TEOST MHT-4 test is capable of measuring phosphorus volatility of engine oils with very good discrimination and precision;it may be considered for use in future ILSAC standards(e.g.GF-5).

Chemical Composition and Allelopathic Activity of <i>Parthenium hysterophorus</i>and <i>Ambrosia polystachya</i>Weeds Essential Oils

The volatile constituents of the essential oils from leaves from the weed plants Parthenium hysterophorus and Ambrosia polystachya were identified and quantified by GLC-MS and GLC. Allelopathic activities were determined by methods that evaluated the volatile effects and the direct application of these essential oils on the seed germination and seedling vigor of lettuce. We identified 27 compounds in the essential oil from P. hysterophorus, and the main constituents were germacrene-D (35.9%), trans-β-ocimene (8.5%) and β-myrcene (7.6%). In the essential oil from A. polystachya, 40 constituents were identified and the principal compounds were germacrene-D (29.3%), trans-β-ocimene (13.6%) and β-caryophyllene (9.8%). In both methods, the essential oil from A. polystachya presented a greater potential for reducing seed germination and seedling vigor in lettuce than the essential oil from P. hysterophorus, and this activity might be attributed to its higher content of monoterpenes.

Essential Oils from Different Parts of the Sea Buckthorn <i>Hippophae rhamnoides</i>L.

The study investigated essential oils of berries and green biomass of the sea buckthorn at various growth stages. Plant material of H. rhamnoídes ssp. mongolica was collected in the experimental fields of the Institute of Cytology and Genetics SB RAS (Siberia, Russia). Volatile compounds and essential oils were detected. Green spring shoots without leaves, first-year seedlings without leaves and berries were analyzed. In all studied exemplars, 103 volatile compounds were defined among which considerable proportion wasn’t found in a sea-buckthorn earlier. Phenylpropanoids prevailed in essential oils composition from seedlings. Alkanes and phenylpropanoids prevailed in shoots. Berries contained mostly carboxylic acids and their esters. These data can be applied to a fingerprint of various cultivars of a sea-buckthorn with relevance to food science and technology.

Chemical Characterization and in Vitro Antitumor Activity of the Essential Oils from the Leaves and Flowers of Callistemon viminalis

The objective of this study was to characterize and verify the in vitro antitumor activity of essential oils (EOs) extracted from the leaves and flowers of Callistemon viminalis. The EOs were extracted by hydrodistillation using a modified Clevenger apparatus. The identification and quantification of constituents were performed on a gas chromatograph coupled to a mass spectrometer and a gas chromatograph with a flame ionization detector. The antitumoral activity was evaluated by a colorimetric assay (MTS) using different cell lines derived from human tumors (breast, lung, glioblastoma, and melanoma). The major constituents of the EOs of leaves and flowers were similar, only quantitative differences being observed. The compounds 1,8-cineole, α-pinene and α-terpineol were found in concentrations of 50.4%, 25.8% and 8.7% in the EOs obtained from the leaves and 48.8%, 24.5% and 3.9% in the EOs obtained from the flowers, respectively. The cytotoxic activity of the EOs was observed only in melanoma cultures (HT144). Cultures treated for 48 h with EOs from leaves and flowers (200 μg·mL-1) reduced the viability by 40% and 25%, respectively. Thus, the antiproliferative activity of the EO from leaves was more pronounced than the EO from flowers in cells derived from melanoma.

Evaluation of Central Nervous System Acting Effects of Plant-Derived Essential Oils Using Ambulatory Activity in Mice

Although plant-derived essential oils (EOs) have traditionally been used for treating various kinds of mental disorders, their Central Nervous System (CNS) acting effects have not yet been clarified well. The present study examined effects of 30 kinds of EOs on ambulatory activity, a kind of spontaneous motor activity, in ICR mice. Nineteen kinds of them did not produce any significant effects on the ambulatory activity of mice. Although effects of EOs of lavender, jasmine and neroli on the activity were statistically significant, their CNS acting properties remained unclear. EOs of peppermint, chamomile, thyme, tea tree, rosemary and basil significantly increased the ambulatory activity of mice as well as CNS stimulants, suggesting that they possess CNS stimulant-like effects. In contrast, an EO of myrtle significantly decreased the activity as well as CNS depressants, suggesting that it possesses a CNS depressant-like effect. An EO of palmarosa significantly increased the activity at a lower dose and decreased at a higher dose as well as some kinds of CNS depressants such as ethanol, suggesting that it also possesses a CNS depressant-like effect. These 8 kinds of EOs might be useful for treating some kinds of mental troubles and/or symptoms through their CNS acting effects.

Use of Essential Oils of the Genus Citrus as Biocidal Agents

In this study, the essential oils extracted from the peels of Citrus aurantifolia, Citrus limon and Citrus sinensis were chemically characterized and quantified. These essential oils and their standards limonene, citral and limonene + citral were evaluated (at concentrations ranging from 500 to 3.91 mL·mL-1) regarding their anti-trypanosome, antifungal and antibacterial activities. The chemical identification and quantification (by GC/FID and GC/MS) displayed the limonene as the major component of the three essential oils. It did not verified anti-trypanosome activity for all treatments. Regarding antimicrobial activity, the results were satisfactory against Gram-negative bacteria (Staphylococcus aureus, Listeria. monocytogenes and Salmonella cholerasuis) and funghi (Aspergillus niger, A. flavus and A. carbonarius), except for the bacterium Pseudomonas aeruginosa, which showed no inhibition for any treatment used.

Application of Fluid Phase Recovery Method in the Early Development of BZ26-6 Volatile Oil Field in Bohai Sea

BZ26-6 Oilfield is a kind of deep metamorphic rock buried-hill volatile oilfield in Bohai Sea, China. Its early development plan is restricted due to the simultaneous production of oil and gas in large sections of reservoirs, unclear understanding of formation fluid properties and uncertainty of gas-oil interface. Through theoretical research on phase recovery and experimental analysis of crude oil phase characteristics in the original formation, characteristic parameters of the equilibrium condensate gas fluid are restored and calculated. Through the superimposed phase diagram of volatile oil and condensate gas, BZ26-6 Oilfield is determined to be a volatile oil reservoir with a condensate gas cap, with formation pressure and saturation pressure of 36.1 MPa, respectively. Based on the research results of oil-gas phase behavior characteristics, the thermodynamic equations and equation of state are jointly used to solve the problem, and the content change curves of each component at different depths are drawn. Combined with the sensitivity analysis of numerical simulation, the gas-oil interface is determined to be -3726 m above sea level. The fluid phase analysis software, Fluidmodeler, is used to simulate volatile oil degassing and condensate gas separation experiments. In combination with oil and gas production data obtained through the production test, the specific oil recovery index and the specific gas recovery index are determined to be 0.408 m3/(MPa·d·m) and 1195 m3/(MPa·d·m), respectively. And the reasonable production capacity prediction is conducted on the early development of BZ26-6 Oilfield. The research results can provide a theoretical basis for the efficient development of similar complex oil and gas reservoirs.

Mechanism of Cinnamomum camphora essential oil for analgesia based on gas chromatography-mass spectrometry integrated network pharmacology

This study aims to explore the analgesic ingredients and mechanism of Cinnamomum camphora essential oil(CCEO).The constituents in CCEO were characterized qualitatively by gas chromatography-mass spectrometry.Targets related to active ingredients were collected by PubChem and Swiss Target Prediction.Targets related to pain were screened by TTD and OMIM database,and compound-target network was established by Cytoscape software.Gene ontology(GO)function and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis of targets were carried out by DAVID database.Protein-protein interaction(PPI)network was established and analyzed by STRING database.Molecular docking method was used to verify the interaction between main components and relevant core targets.A total of 13 compounds were identified in CCEO,and 58 related targets were predicted.GO function enrichment analysis revealed that the selected targets were mainly involved in biological processes such as chemical synaptic transmission and molecular function such as neurotransmitter receptor activity;24 signal pathways were screened by KEGG pathway enrichment analysis,including neuroactive ligand-receptor interaction,retrograde endocannabinoid signaling and calcium signaling pathway.Docking results showed that the main constituents had certain affinities with the key targets.The active ingredients in CCEO regulated multiple signaling pathways to ameliorate pain through AR,ACHE,ESR1,GABRG2,PTGS2 and PPARγ.

Comparative analysis of volatile oils in the stems and roots of Ephedra sinica via GC-MS-based plant metabolomics

With a great difference in therapeutic effects of Mahuang(MH, the stems of Ephedra sinica) and Mahuanggen(MHG, the roots of Ephedra sinica), chemical differences between MH and MHG should be investigated. In the present study, gas chromatography-mass spectrometry(GC-MS)-based plant metabolomics was employed to compare volatile oil profiles of MH and MHG. The antioxidant activities of volatile oils from MH and MHG were also compared. 32 differential chemical markers were identified according to the variable importance in the projection(VIP) value of orthogonal partial least squares discriminant analysis(OPLS-DA) and P value of Mann-Whitney test. Among them, chemical markers of tetramethylpyrazine(TMP) and α-terpineol were quantified. Their contents were much higher in most MH samples compared with MHG. The antioxidant assay demonstrated that MH had significantly higher free radical-scavenging activity than MHG. Although MH and MHG derived from the same medicinal plant, there was much difference in their volatile oil profiles. MH samples had significantly higher content of two reported pharmacologically important chemical markers of TMP and α-terpineol, which may account for their different antioxidant activities.

Network pharmacology deciphering multiple mechanisms of volatiles of Wendan granule for treatment of senile dementia

OBJECTIVE To explore the mechanisms of the volatiles of Wendan granule for the treatment of senile dementia,network pharmacology method integrating absorption,distribution,metab.olism,and excretion(ADME) screening,target fishing,network constructing,pathway analyzing,and correlated diseases prediction was applied.METHODS Twelve small molecular compounds of WDG were selected as the objects from 74 volatiles with the relative abundances above 2%,and their ADME parameters were collected from Traditional Chinese Medicine Systems Pharmacology platform(TCMSP),and then the corresponding targets,genes,pathways and diseases were predicted according to the data provided by TCMSP,DrugBank,Uniport and the Database for Annotation,Visualization and Integrated Discovery(DAVID).The related pathways and correlation analysis were explored by the Kyoto Encyclo.pedia and Genomes(KEGG) database.Finally,the networks of compound-target,target-pathway and pathway-disease of WDG were constructed by Cytoscape software.RESULTS Twelve compounds interacted with 49 targets,of which top three targets were Gamma-aminobutyric acid receptor subunit alpha-1(GABRA1),Prostaglandin G/H synthase 2(PGHS-2) and Sodium-dependent noradrenaline transporter.Interestingly,these targets were highly associated with depression,insomnia and Alzheimer′s disease that mainly corresponded to mental and emotional illnesses.CONCLUSION The integrated network pharmacology method provides precise probe to illuminate the molecular mechanisms of volatiles of WDG for relieving senile dementia related syndromes,which will also facilitate the application of traditional Chinese medicine in modern medicine,as well as follow-up studies such as upgrading the quality stan.dard of clinical medicine and novel drug development.

Pharmacological effects of volatileoil from Alpinia officinaruim in treating cardiovascular diseases

Galangal(Alpinia officinaruim Hance)is the rhizome of the perennial herb belonging to Zingiberaceae family.There are many active components in galangal,such as volatile oil,flavonoids,terpenoids,phenylpropanoids and glycosides,among which the content of volatile oil is higher.The bioactivities of galangal volatile oil on health effect includesanti-inflammatory,anti-hypertension,anti-oxidation and prevention of cardiovascular diseases.Cardiovascular disease(CVD)is a kind of diseases related to circulatory system,which is also called circulatory system diseases.Over the past decade,the number of people dying from CVD has increased by 12.5%worldwide,and it is now the leading cause of human death worldwide.Studies have shown that galangal volatile oil has good pharmacological effects in treating CVD.①Regulation of glucose and lipid metabolism:studies have found that abnormal lipid metabolism can lead to obesity,diabetes,CVD and other diseases.The serum total triglyceride(TG)content in liver and serum will increase in patients with abnormal fat metabolism.The results showed that the volatile oil of galangal could increase the excretion of neutral cholesterol,significantly reduce liver TG and serum TG,and thus regulate glucose and lipid metabolism,prevent lipid deposition and prevent CVD.②Improving insulin resistance(IR):inhibition of inflammatory cytokines such as IL^(-1),IL-6 activation and expression of TNF-α,improves IR,thereby protecting myocardium from IR-mediated damage.Through the establishment of endothelial cell injury model induced by high glucose in vitro,it was found that the volatile oil of galangal can significantly reduce the secretion of pro-inflammatory cytokines TNF-αand IL-8,and inhibit the expression of ICAM-1 and VCAM-1 induced by high glucose,suggesting that it has protective effect on endothelial dysfunction and inflammation induced by high glucose.③Regulate blood oxygenation:during acute myocardial hypoxia,the activity of free radical scavenging system is decreased,and oxygen free radicals are produced in large quantity,which reacts with unsaturated fatty acids on the cell membrane and forms lipid peroxidation,resulting in myocardial structural damage.The results showed that the water extract of Galangal could reduce the content of MDA in blood and protect the SOD activity of ischemic and hypoxic myocardium.④Protective effect of vascular endothelial cells(ES):ES injury is the pathological basis of some cardiovascular diseases.The results showed that the volatile oil of galangal had a protective effect on ES apoptosis.Compared with the morphology and activity of ES treated with oxidized LDL,galangal volatile oil could ameliorate these morphological changes and improve cell viability.⑤Antiplatelet agglutination:inhibit platelet aggregation and thromboxane release,improve blood circulation,and have obvious anti-thrombotic effect,which has a good effect on the treatment and prevention of cardiovascular diseases.The results showed that the volatile oil of galangal had inhibitory effect on platelet aggregation and anticoagulant effect.In conclusion,the volatile oil of galangal can be used to prevent and treat cardiovascular diseases.Based on the mechanism of CVD,this study summarized the role of the essential oil of Alpinia officinaruim in CVD,providing basis for the clinical application of alpiniaofficinalis essential oil in the prevention and treatment of CVD and the development of new drugs.