Objective:To explore the effect of okra extract on gestational diabetes mellitus(GDM) rats and its probable molecular mechanism.Methods:A total of 30 female SD rats were caged with male rats for pregnancy,27 pregnant ...Objective:To explore the effect of okra extract on gestational diabetes mellitus(GDM) rats and its probable molecular mechanism.Methods:A total of 30 female SD rats were caged with male rats for pregnancy,27 pregnant rats were obtained and weighed.The pregnant rats were equally randomized into the control group,GDM group and intervention group.Once the pregnancy was verified,GDM group and intervention group were given 45 mg/kg streptozotocin by peritoneal injection for inducing GDM,control group was given equal volume of citrate buffer.Once the model was established successfully,intervention group was administered orally the solution containing 200 mg/kg/d okra extract,the other groups were given the diet and water only.On the 19 th day of pregnancy,the blood samples and fetal rats of all groups were collected,fetal rats weight and placental weight was recorded and the serum glucose,lipids,serum insulin and C-peptide of pregnant rats before the delivery were determined.Results:The pregnant rats weight before the delivery,fetal rats weight and placental weight of GDM group were lower than control group and intervention group(P<0.05).After the treatment of okra extract,serum glucose and lipids levels of intervention group were both improved significantly(P<0.05),especially,the FBG,HDL,FINS,serum m insulin and hepatic glycogen levels were equivalent to control group(P>0.05).Antioxidant enzymes levels of GDM group in liver and pancreas tissues were lower than the other groups,and after treatment of okra extract,antioxidant enzymes levels in liver and pancreas tissues were equivalent to control group(P>0.05).Conclusions:Okra extract,rich in antioxidant substances,could avoid the excessive consuming of antioxidant enzymes,then,suppresses the oxidative stress and insulin resistance,thereby improving blood glucose level of GDM rats.展开更多
Convolvulus arvensis is a toxic allelopathic weed that suppresses germination and growth of crops. The prime object of present study was to investigate effect of Convolvulus arvensis water extract on germination and p...Convolvulus arvensis is a toxic allelopathic weed that suppresses germination and growth of crops. The prime object of present study was to investigate effect of Convolvulus arvensis water extract on germination and performance of okra with different seed sizes. The seeds of okra variety pusa green were separated into three different sizes, viz large size （4.00-5.00 mm）, medium size （3.00-3.50 mm） and small size （2.00-3.50 mm）, and then soaked in allelopathic plant bindweed water extract and kept in patrisdishes for germination into the germinator at 15℃. The experiment was laid out using Complete Randomized Design （CRD） with three replications. The results showed that after sowing of 12 days the highest germination was observed in non-treated seeds as compared to seeds treated in Convolvulus arvensis water extract for 1 h, further non-treated large seeds produced maximum plants as compared to treated small seeds after sowing of 24 days. Meanwhile, root length, shoot length, root fresh and dry weight, shoot fresh and dry weight were recorded higher in non-treated large seeds as compared to small seeds soaked for 1 h in Convolvulus arvensis allelopthic water extract. It could be found that Convolvulus arvensis affected germination, seed growth and overall performance of okra, further presence Convolvulus arvensis in crops could cause negative impact on germination and integrity of okra crops.展开更多
The insecticidal efficacy of aqueous extracts of R. communis L. and Z. officinales L. was tested in a field study for the control of two important leaf defoliators （Podagrica uniformis and Nisotra sjostedti of okra）...The insecticidal efficacy of aqueous extracts of R. communis L. and Z. officinales L. was tested in a field study for the control of two important leaf defoliators （Podagrica uniformis and Nisotra sjostedti of okra）. The extracts were applied at 10% （w/v） and sprayed every week for five consecutive weeks. R. communis significantly reduced （P〈0.05） abundance of the pests on the 2nd day after spraying and increased the yield of okra. Numbers of fruits and weight yields were significantly higher in plots treated with R. communis compared to plots treated with Z. officinales and the untreated control. R. communis had the same weight yield with Karate （synthetic insecticides）. The results indicated that R. communis could be used to control Podagrica uniformis and Nisotra sjostedti of okra.展开更多
以秋葵嫩荚为原料,通过超声浸提法制备提取液,然后采用盐酸萘乙二胺分光光度法测定提取液对亚硝酸盐的清除率。通过单因素试验及正交试验分析提取溶剂、提取液用量、反应时间、pH值4个因素对亚硝酸盐清除作用的影响,并以维C为阳性对照...以秋葵嫩荚为原料,通过超声浸提法制备提取液,然后采用盐酸萘乙二胺分光光度法测定提取液对亚硝酸盐的清除率。通过单因素试验及正交试验分析提取溶剂、提取液用量、反应时间、pH值4个因素对亚硝酸盐清除作用的影响,并以维C为阳性对照。结果表明,秋葵提取液清除亚硝酸盐的最佳条件为提取溶剂为体积分数65%的乙醇,提取液用量2.5 m L,反应时间1.5 h,pH值2.0,该条件下测得提取液对亚硝酸盐的清除率为90.99%。通过与维C进行对比分析,得出秋葵提取液对亚硝酸盐具有较好的清除能力。展开更多
文摘Objective:To explore the effect of okra extract on gestational diabetes mellitus(GDM) rats and its probable molecular mechanism.Methods:A total of 30 female SD rats were caged with male rats for pregnancy,27 pregnant rats were obtained and weighed.The pregnant rats were equally randomized into the control group,GDM group and intervention group.Once the pregnancy was verified,GDM group and intervention group were given 45 mg/kg streptozotocin by peritoneal injection for inducing GDM,control group was given equal volume of citrate buffer.Once the model was established successfully,intervention group was administered orally the solution containing 200 mg/kg/d okra extract,the other groups were given the diet and water only.On the 19 th day of pregnancy,the blood samples and fetal rats of all groups were collected,fetal rats weight and placental weight was recorded and the serum glucose,lipids,serum insulin and C-peptide of pregnant rats before the delivery were determined.Results:The pregnant rats weight before the delivery,fetal rats weight and placental weight of GDM group were lower than control group and intervention group(P<0.05).After the treatment of okra extract,serum glucose and lipids levels of intervention group were both improved significantly(P<0.05),especially,the FBG,HDL,FINS,serum m insulin and hepatic glycogen levels were equivalent to control group(P>0.05).Antioxidant enzymes levels of GDM group in liver and pancreas tissues were lower than the other groups,and after treatment of okra extract,antioxidant enzymes levels in liver and pancreas tissues were equivalent to control group(P>0.05).Conclusions:Okra extract,rich in antioxidant substances,could avoid the excessive consuming of antioxidant enzymes,then,suppresses the oxidative stress and insulin resistance,thereby improving blood glucose level of GDM rats.
文摘Convolvulus arvensis is a toxic allelopathic weed that suppresses germination and growth of crops. The prime object of present study was to investigate effect of Convolvulus arvensis water extract on germination and performance of okra with different seed sizes. The seeds of okra variety pusa green were separated into three different sizes, viz large size （4.00-5.00 mm）, medium size （3.00-3.50 mm） and small size （2.00-3.50 mm）, and then soaked in allelopathic plant bindweed water extract and kept in patrisdishes for germination into the germinator at 15℃. The experiment was laid out using Complete Randomized Design （CRD） with three replications. The results showed that after sowing of 12 days the highest germination was observed in non-treated seeds as compared to seeds treated in Convolvulus arvensis water extract for 1 h, further non-treated large seeds produced maximum plants as compared to treated small seeds after sowing of 24 days. Meanwhile, root length, shoot length, root fresh and dry weight, shoot fresh and dry weight were recorded higher in non-treated large seeds as compared to small seeds soaked for 1 h in Convolvulus arvensis allelopthic water extract. It could be found that Convolvulus arvensis affected germination, seed growth and overall performance of okra, further presence Convolvulus arvensis in crops could cause negative impact on germination and integrity of okra crops.
文摘The insecticidal efficacy of aqueous extracts of R. communis L. and Z. officinales L. was tested in a field study for the control of two important leaf defoliators （Podagrica uniformis and Nisotra sjostedti of okra）. The extracts were applied at 10% （w/v） and sprayed every week for five consecutive weeks. R. communis significantly reduced （P〈0.05） abundance of the pests on the 2nd day after spraying and increased the yield of okra. Numbers of fruits and weight yields were significantly higher in plots treated with R. communis compared to plots treated with Z. officinales and the untreated control. R. communis had the same weight yield with Karate （synthetic insecticides）. The results indicated that R. communis could be used to control Podagrica uniformis and Nisotra sjostedti of okra.
文摘以秋葵嫩荚为原料,通过超声浸提法制备提取液,然后采用盐酸萘乙二胺分光光度法测定提取液对亚硝酸盐的清除率。通过单因素试验及正交试验分析提取溶剂、提取液用量、反应时间、pH值4个因素对亚硝酸盐清除作用的影响,并以维C为阳性对照。结果表明,秋葵提取液清除亚硝酸盐的最佳条件为提取溶剂为体积分数65%的乙醇,提取液用量2.5 m L,反应时间1.5 h,pH值2.0,该条件下测得提取液对亚硝酸盐的清除率为90.99%。通过与维C进行对比分析,得出秋葵提取液对亚硝酸盐具有较好的清除能力。
