Objective:To investigate the intervention of Xuduan Zhongzi Formula on the epididymis structure of model of oligospermia mice.Methods:Ten of the 45 male mice were labeled as the normal group,and the remaining 35 mice ...Objective:To investigate the intervention of Xuduan Zhongzi Formula on the epididymis structure of model of oligospermia mice.Methods:Ten of the 45 male mice were labeled as the normal group,and the remaining 35 mice were injected with chloral hydrate for five consecutive days,and the randomly selected five mice were anesthetized with chloral hydrate.The left epididymis was removed,and a few sperm were found in the epididymis under the optical microscope,indicating successful construction of the model.They were randomly divided into three groups:the normal group,the L-carnitine group and the Xuduan group with 10 rats in each group.The the L-carnitine group and the Xuduan group were administrated with equivalent dose of human solvent,and the normal group and the model group were administrated with normal saline.After 8 weeks of intragastric administration,all experimental mice were killed,and the left epididymis was extracted for sperm detection,seminal plasma biochemical detection,HE staining,and electron microscopy.Results:In the model group,enlarged epididymal epithelial cells,vacuolar degeneration of primary and basal cells,and edema of interstitial cells and vascular dilation were found.Compared with the normal group,semen concentration,activity,SOD,A-glucosidase and fructose in the model group were significantly decreased,and the difference was statistically significant(P<0.01).After eight weeks of drug intragastric treatment,the semen concentration,activity,SOD,A-glucosidase and fructose in the Xuduan group were significantly increased compared with the model group,and the difference was statistically significant(P<0.01).Conclusion:Xuduan Zhongzi Formula can significantly improve the epididymal structure,the microenvironment of epididymal sperm maturation and the stability of epididymal epithelial structure of oligospermia model mice,creating conditions for sperm maturation in the epididymis.展开更多
Objective:Exploring the material basis and mechanism of Kidney-invigorating and Spleenstrengthening method for treating oligospermia based on network pharmacology.Method:Select the effective elements and targets of Yi...Objective:Exploring the material basis and mechanism of Kidney-invigorating and Spleenstrengthening method for treating oligospermia based on network pharmacology.Method:Select the effective elements and targets of Yishen Jianpi core drug pairs by searching the Chinese Medicine System Pharmacology Platform(TCMSP);select the disease targets of oligozoospermia by searching the human genetic information database;use R language software to select drugs and Disease common target,use Cytoscape software to build a drugdisease-target interaction network;build a common target protein interaction network(PPI)through the STRING platform;apply R language to the core drugs of Yishen Jianpi and oligospermia Enrichment analysis of GO and KEGG was performed on the common target.Result:A total of 97 effective active ingredients were obtained from Yishen Jianpi traditional Chinese medicine,and 46 possible targets for Yishen Jianpi traditional Chinese medicine to treat oligospermia were obtained.Combined with the results of KEGG enrichment analysis,it can be speculated that Yishen Jianpi traditional Chinese medicine improved male oligospermia The whole mechanism of action may be related to the regulation of human endogenous hormones.Conclusion:Through the research method of network pharmacology,the possible effect targets and pathways of Yishen Jianpi traditional Chinese medicine in the treatment of male oligospermia are predicted.展开更多
A man having severe oligospermia,due to partial maturation arrest at spermatid stage,was given low dose estrogen-testosterone conbination therapy for three months.His sperm count increased enormously,following which h...A man having severe oligospermia,due to partial maturation arrest at spermatid stage,was given low dose estrogen-testosterone conbination therapy for three months.His sperm count increased enormously,following which his wife conceived and delivered a healthy baby at term.展开更多
本研究基于环磷酰胺致少精症斑马鱼模型,考察了达迷草叶黑胡椒提取物(extract of damiana and black pepper,EDBP)的改善作用。EDBP最大耐受浓度(maximum tolerance concentration,MTC)测定为500μg/mL。将10 mpf雄性斑马鱼随机分为模...本研究基于环磷酰胺致少精症斑马鱼模型,考察了达迷草叶黑胡椒提取物(extract of damiana and black pepper,EDBP)的改善作用。EDBP最大耐受浓度(maximum tolerance concentration,MTC)测定为500μg/mL。将10 mpf雄性斑马鱼随机分为模型对照组、阳性药物组、EDBP-250μg/mL组和EDBP-500μg/mL组,另设正常对照组。各给药组浸浴处理8日,加入环磷酰胺同时处理8日。测定斑马鱼生长发育参数、精子质量、血浆激素水平、精巢组织CYP17α2、lhcgr和fshr的核酸表达水平。结果显示,与正常对照组比较,模型对照组斑马鱼体长、体重、精巢重量、精子活力、精子数量显著降低,血浆睾酮(testosterone,T)、卵泡刺激素(follicle-stimulating hormone,FSH)和黄体生成素(luteinizing hormone,LH)水平显著下降(p<0.001),精巢组织中CYP17α2、lhcgr和fshr表达水平显著降低。EDBP组测定结果较模型对照组的各项指标不同程度升高,其中500μg/mL剂量组更优。综合结果表明达迷草叶黑胡椒提取物具有明显的改善斑马鱼少精症的作用。展开更多
基金Central Government Funds for Guiding Local Science and Technology Development(No.ZY20198022)Graduate Education Innovation Program of Guangxi University of Traditional Chinese Medicine(No.YCXJ2021067)。
文摘Objective:To investigate the intervention of Xuduan Zhongzi Formula on the epididymis structure of model of oligospermia mice.Methods:Ten of the 45 male mice were labeled as the normal group,and the remaining 35 mice were injected with chloral hydrate for five consecutive days,and the randomly selected five mice were anesthetized with chloral hydrate.The left epididymis was removed,and a few sperm were found in the epididymis under the optical microscope,indicating successful construction of the model.They were randomly divided into three groups:the normal group,the L-carnitine group and the Xuduan group with 10 rats in each group.The the L-carnitine group and the Xuduan group were administrated with equivalent dose of human solvent,and the normal group and the model group were administrated with normal saline.After 8 weeks of intragastric administration,all experimental mice were killed,and the left epididymis was extracted for sperm detection,seminal plasma biochemical detection,HE staining,and electron microscopy.Results:In the model group,enlarged epididymal epithelial cells,vacuolar degeneration of primary and basal cells,and edema of interstitial cells and vascular dilation were found.Compared with the normal group,semen concentration,activity,SOD,A-glucosidase and fructose in the model group were significantly decreased,and the difference was statistically significant(P<0.01).After eight weeks of drug intragastric treatment,the semen concentration,activity,SOD,A-glucosidase and fructose in the Xuduan group were significantly increased compared with the model group,and the difference was statistically significant(P<0.01).Conclusion:Xuduan Zhongzi Formula can significantly improve the epididymal structure,the microenvironment of epididymal sperm maturation and the stability of epididymal epithelial structure of oligospermia model mice,creating conditions for sperm maturation in the epididymis.
基金“Experience Inheritance 3+3 Project”of the renown Chinese medicine physician studio of Wang Pei of Beijing university of Chinese medicine Dongzhimen Hospital branchStudy on the clinical efficacy and mechanism of kidney–Nurturing classical prescriptions based on the theory of“Kidney stores energy and governs water”Project of Enn group award fund f Beijing university of Chinese medicine.
文摘Objective:Exploring the material basis and mechanism of Kidney-invigorating and Spleenstrengthening method for treating oligospermia based on network pharmacology.Method:Select the effective elements and targets of Yishen Jianpi core drug pairs by searching the Chinese Medicine System Pharmacology Platform(TCMSP);select the disease targets of oligozoospermia by searching the human genetic information database;use R language software to select drugs and Disease common target,use Cytoscape software to build a drugdisease-target interaction network;build a common target protein interaction network(PPI)through the STRING platform;apply R language to the core drugs of Yishen Jianpi and oligospermia Enrichment analysis of GO and KEGG was performed on the common target.Result:A total of 97 effective active ingredients were obtained from Yishen Jianpi traditional Chinese medicine,and 46 possible targets for Yishen Jianpi traditional Chinese medicine to treat oligospermia were obtained.Combined with the results of KEGG enrichment analysis,it can be speculated that Yishen Jianpi traditional Chinese medicine improved male oligospermia The whole mechanism of action may be related to the regulation of human endogenous hormones.Conclusion:Through the research method of network pharmacology,the possible effect targets and pathways of Yishen Jianpi traditional Chinese medicine in the treatment of male oligospermia are predicted.
文摘A man having severe oligospermia,due to partial maturation arrest at spermatid stage,was given low dose estrogen-testosterone conbination therapy for three months.His sperm count increased enormously,following which his wife conceived and delivered a healthy baby at term.
文摘本研究基于环磷酰胺致少精症斑马鱼模型,考察了达迷草叶黑胡椒提取物(extract of damiana and black pepper,EDBP)的改善作用。EDBP最大耐受浓度(maximum tolerance concentration,MTC)测定为500μg/mL。将10 mpf雄性斑马鱼随机分为模型对照组、阳性药物组、EDBP-250μg/mL组和EDBP-500μg/mL组,另设正常对照组。各给药组浸浴处理8日,加入环磷酰胺同时处理8日。测定斑马鱼生长发育参数、精子质量、血浆激素水平、精巢组织CYP17α2、lhcgr和fshr的核酸表达水平。结果显示,与正常对照组比较,模型对照组斑马鱼体长、体重、精巢重量、精子活力、精子数量显著降低,血浆睾酮(testosterone,T)、卵泡刺激素(follicle-stimulating hormone,FSH)和黄体生成素(luteinizing hormone,LH)水平显著下降(p<0.001),精巢组织中CYP17α2、lhcgr和fshr表达水平显著降低。EDBP组测定结果较模型对照组的各项指标不同程度升高,其中500μg/mL剂量组更优。综合结果表明达迷草叶黑胡椒提取物具有明显的改善斑马鱼少精症的作用。
