Land subsidence is a major factor that affects metro line (ML) stability. In this study, an improved multi- temporal interferometric synthetic aperture radar (InSAR) (MTI) method to detect land subsidence near M...Land subsidence is a major factor that affects metro line (ML) stability. In this study, an improved multi- temporal interferometric synthetic aperture radar (InSAR) (MTI) method to detect land subsidence near MLs is presented. In particular, our multi-temporal InSAR method provides surface subsidence measurements with high observation density. The MTI method tracks both point-like targets and distributed targets with temporal radar back- scattering steadiness. First, subsidence rates at the point targets with low-amplitude dispersion index (ADI) values are extracted by applying a least-squared estimator on an optimized freely connected network. Second, to reduce error propagation, the pixels with high-ADI values are classified into several groups according to ADI intervals and processed using a Pearson correlation coefficient and hierarchical analysis strategy to obtain the distributed targets. Then, nonlinear subsidence components at all point-like and distributed targets are estimated using phase unwrapping and spatiotemporal filtering on the phase residuals. The proposed MTI method was applied to detect land subsidence near MLs of No. 1 and 3 in the Baoshan district of Shanghai using 18 TerraSAR-X images acquired between April 21, 2008 and October 30, 2010. The results show that the mean subsidence rates of the stations distributed along the two MLs are -12.9 and -14.0 ram/year. Furthermore, three subsidence funnels near the MLs are discovered through the hierarchical analysis. The testing results demonstrate the satisfactory capacity of the proposed MTI method in providing detailed subsidence information near MLs.展开更多
Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stab...Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant reiroviral vector-mediated gene transfer method.The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide(MTT)and cell colony formation assays.eyclnD1,p21^(cipt)and p27^(kipt)mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR.cyclinD1,p21^(cipt),p27^(kipt),AKT,p-AKT,FOXOt and p-FOX01 expressions in the transfected Tca8113 were detected by western blot analysis.In addition,miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.Results:Quantitative PCR showed that mitt-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue.MTT and cell colony formation assays showed that after miR-25-3p overexpression,the proliferation of transfected Tca8113 was obviously attenuated.Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression.p21^(cipt)and p27^(kipt)expressions were upregulated,while cyclinD1,AKT,FOXO1 expressions were downregulated,and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.Conclusions:MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression,playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.展开更多
We report the discovery of a broad absorption line (BAL) of - 10^4 km s^-1 in width in the previously known BL Lac object PKS 0138-097, which we tentatively identified as an Mg II BAL. This is the first detection of...We report the discovery of a broad absorption line (BAL) of - 10^4 km s^-1 in width in the previously known BL Lac object PKS 0138-097, which we tentatively identified as an Mg II BAL. This is the first detection of a BAL, which is sometimes seen in powerful quasars with high accretion rates, in a BL Lac object. The BAL was clearly detected in its spectra spanning two epochs at a high luminosity state taken in the Sloan Digital Sky Survey (SDSS), while it disappeared in three SDSS spectra taken at a low luminosity state. The BAL and its variability pattern were also found in its historical multi-epoch spectra in the literature, but have been overlooked previously. In its high resolution radio maps, PKS 0138-097 shows a core plus a one- sided parsec-scale jet. The BAL variability can be interpreted as follows: The optical emission is dominated by the core in a high state and by the jet in a low state and the BAL material is located between the core and jet so that the BAL appears only when the core is shining. Our discovery suggests that outflows may also be produced in active galactic nuclei at a low accreting state.展开更多
We analyze the spectral line broadening of those magneto-sensitive lines in solar magnetized atmospheres. The broadening at the line wings is due to the increase of the effective width of energy levels involved in Zee...We analyze the spectral line broadening of those magneto-sensitive lines in solar magnetized atmospheres. The broadening at the line wings is due to the increase of the effective width of energy levels involved in Zeeman splitting,and the broadening at the line core also originated in Zeeman splitting under the condition that the Zeeman components are mixed. Therefore, the magneto-induced or Zeeman broadening take effects on the whole line. The observed Stokes parameter data in a sunspot and outside it acquired by Solar Stokes Spectrum Telescope (S^3T) are analyzed for the demonstration of this mechanism, and the Zeeman broadening rates are calculated for Fe16302.5 under some assumptions.Our result shows that the broadening is increased as the magnetic field strength becomes stronger, but the rate of increase at the line core is decreased as the field strength increases, while the rate at the wing does not show such an obvious regularity. The broadening is more effective in the line core than in the wings.展开更多
The line profiles of Ha in a limb flare on 1998 November 11 appear to be unusually broadened. It is considered that macro-turbulence (or macroscopic mass motions) may be one of the main causes. We use an inversion tec...The line profiles of Ha in a limb flare on 1998 November 11 appear to be unusually broadened. It is considered that macro-turbulence (or macroscopic mass motions) may be one of the main causes. We use an inversion technique to extract the probability distribution of the line-of-sight velocity in the flare. There exist some differences between the velocity distributions deduced from Ha and from Ca II λ8542, which may be because the two lines depend differently on the temperature and velocity. Since the loop density is high, we obtain a rather short cooling time (several tens of seconds) from the hot X-ray loops to the cool loops visible in Ha. Possible origins of the large scale motions are discussed.展开更多
For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the ef...For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the effect of gastric carcinoma cell differentiation by chemical inducers.Under light microscope, MGc80-3 cells, after treated with 1 mM dBcAMP, tended to be flat and disperse, and their volume gradually enlarged, with their uncleus relatively smaller and their shape rather regular. Morphological changes, like norma differentiated epithelial cells, were observed. The cells attached firmly, grew slowly, their growth curve showed inhibitory rate amounted to 52.87%, and cellular division exponent displayed their peak value 1.5 times less than that of MGc80-3 cells. It was clear that dBcAMP could effectively inhibit the multiplication activity of MGc80-3 cells. After dBcAMP treatment, remarkable changes of cell surface charges was indicated by cell electrophoresis, the ratio dropped to 3.043 from 3.988, and their re-tardant ratio reached up to 31.2%. cAMP content in cells after this treatment, detected by cAMP and cGMP radioimmunoassay, was enhanced by 2.42 times, and cAMP/cGMP ratio, by 1.73 times. Thus, cAMP level within MGc80-3 cells was raised obviously by dBcAMP. Heterotransplantation experiments showed that tuntorigenic rate of MGc80-5 cells (transplanted subcutaneously to BALB/c mice) amounted to 100%, and that of the cells after this treatment was only 5.6%. Their tumorigenic ability was extremely reduced.These results confirmed that dBcAMP was able to change malignant phenotypic characteristics of MGc80-3 cells and produce a reversed alteration: Thus, it has a remarkable inductive effect in differentiating gastric carcinoma cells. All these characteristics were also considered as the reference indexes in appraising reversed effect for the homologous cancer cells.展开更多
In order to search for intensity fluctuations on the HCN(1-0) and HCO+(1-0) line pro- files, which could arise due to possible small-scale inhomogeneous structure, long-term observations of high-mass star-forming...In order to search for intensity fluctuations on the HCN(1-0) and HCO+(1-0) line pro- files, which could arise due to possible small-scale inhomogeneous structure, long-term observations of high-mass star-forming cores S140 and S199 were carried out. The data were processed by the Fourier filtering method. Line temperature fluctuations that exceed the noise level were detected. Assuming the cores consist of a large number of randomly moving small thermal fragments, the total number of frag- ments is - 4 × 106 for the region with linear size - 0.1 pc in S140 and - 106 for the region with linear size - 0.3 pc in S 199. Physical parameters of fragments in S 140 were obtained from detailed modeling of the HCN emission in the framework of the clumpy cloud model.展开更多
A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant an...A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5' AMPase, ACP etc.) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on day 5 with mitotic index reaching 1.8% on day 3. This cell line was a hyperdiploid with karyotype of 45 or 45, -2X, tril2, tri17, +M1-5. Cell agglutination was observed when treated with ConA (≥7 fig, ml). Spontaneous agglutination might also take place without adding any ConA. After 5×106 cells of Ca 761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutan eous, intrafoot-pad or intraperitoneal), the transplan lability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca 761-86 may be of value for practical purposes.展开更多
In order to study the effect of 5, 6-Dichloro-l-13-D-ribofuranosyl-benzimidazole (DRB) on the biological characteristics of human laryngeal carcinoma Hep-2 cell line in vitro, Hep-2 cells cultured in vitro were trea...In order to study the effect of 5, 6-Dichloro-l-13-D-ribofuranosyl-benzimidazole (DRB) on the biological characteristics of human laryngeal carcinoma Hep-2 cell line in vitro, Hep-2 cells cultured in vitro were treated with different concentrations of DRB. Changes in cell proliferation, apoptotic rate and invasiveness were detected by MTT assay, flow cytometry (FCM) and matrigel in vitro invasion assay, respectively. It was found that DRB inhibited the proliferation of Hep-2 cells in a dose- and time-dependent manner. After being treated with 0, 10, 20, 40, 80 μmmol/L DRB for 24 h, the apoptotic rate in Hep-2 cells was (0.68±0.19)%, (1.95±0.12)%, (8.51±0.26)%, (11.26±0.17)% and (14.99±0.32)%, respectively. The matrigel in vitro invasion assay revealed that DRB began to inhibit the invasion of Hep-2 cells at the concentration of 5 μmmol/L, and with the increase of DRB concentration, the inhibitory effect was enhanced. It was suggested that DRB could influence the essential biological characteristics of Hep-2 cells, inhibit Hep-2 cells proliferation, reduce invasive ability and induce apoptosis of Hep-2 cells.展开更多
Emission lines from the broad emission line region (BELR) and the narrow emission line region (NELR) of active galactic nuclei (AGNs) have been extensively studied. However, emission lines are rarely detected be...Emission lines from the broad emission line region (BELR) and the narrow emission line region (NELR) of active galactic nuclei (AGNs) have been extensively studied. However, emission lines are rarely detected between these two regions. We present a detailed analysis of quasar SDSS J232444.80-094600.3 (SDSS J2324-0946), which is remarkable for its strong intermediate-width emission lines (IELs) with FWHM ≈ 1800 km s^-1. The IEL component is present in different emission lines, including the permitted lines Lyαλ1216, CⅣ λ1549, semiforbidden line C Ⅲ] λ1909, and forbidden lines [OⅢ] ss4959, 5007. With the aid of photo-ionization models, we found that the IELs are produced by gas with a hydrogen density of nH - 10^6.2 -- 10^6.3 cm^-3, a distance from the central ionizing source of R - 35 - 50 pc, a covering factor of - 6%, and a dust-to-gas ratio of ≤ 4% that of the SMC. We suggest that the strong IELs of this quasar are produced by nearly dust-free and intermediate-density gas located at the skin of the dusty torus. Such strong IELs, which serve as a useful diagnostic, can provide an avenue to study the properties of gas between the BELR and the NELR.展开更多
We present an exercise that intends to establish a relationship between the strength of nebular emission lines and optical stellar features in the spectrum of a galaxy. After accurately subtracting the stellar continu...We present an exercise that intends to establish a relationship between the strength of nebular emission lines and optical stellar features in the spectrum of a galaxy. After accurately subtracting the stellar continuum and the underlying stellar absorption, we made reliable measurements of the emission lines of all the galaxies in the Sloan Digital Sky Survey Data Release 2 (SDSS DR2). More than 4000 star-forming galaxies with high S/N ratio of both the stellar spectrum and the emission lines are selected. These galaxy spectra are fitted with the 10 PCs of Yip et al., after all the emission line regions have been filtered out. We find that the flux of hydrogen Balmer emission lines, Ha and Hβ can be well recovered from the PCs, while the metal lines are not well reproduced. The fluxes of Ha and Hβ measured from the PC-reconstructed spectra and from the observed spectra agree well with an rms scatter of only - 0.1 dex. This result suggests that, with moderate spectral resolution and S/N ratio, the optical stellar spectrum of a galaxy can serve as an indicator of star formation rate.展开更多
Line surveys of complex molecules with millimeter and sub-millimeter telescopes are important for probing the physical and chemical environments of massive star forming regions(MSFRs).We present a molecular line surve...Line surveys of complex molecules with millimeter and sub-millimeter telescopes are important for probing the physical and chemical environments of massive star forming regions(MSFRs).We present a molecular line survey with the Submillimeter Array(SMA) in the frequency ranges of 220.3–222.3 GHz and 230.3–232.3 GHz toward G10.6-0.4, the brightest star forming core in the W31 complex. Ninety-nine transitions from 22 molecular species and their isotopologues are identified. The moment 0 images of typical molecules show a compact core which is concentrated at the continuum peak position. Based on the local thermodynamic equilibrium assumption, the molecular line data are modeled. The rotational temperatures of those molecular species range from 96 to 178 K and their column densities range from 2.0×1014to 3.7×1017cm-2. The observational data suggest that all complex molecules are located in a warm environment. Chemical environments of the molecules are discussed. We compared molecular abundances and gas temperatures in G10.6-0.4 with those in other MSFRs, and found that gas temperatures and fractional abundances of specific molecules in G10.6-0.4 are similar to the typical MSFR W51 North, suggesting that there are similar physical and chemical environments in these two MSFRs.展开更多
In order to detect molecular markers for the epidermal growth factor inhibitor 4-(3-chloro-benzyl)- 6,7-dimethoxy-quinazoline (tyrphostin), we investigated the kinetics of p120-catenin and periplakin in the human bucc...In order to detect molecular markers for the epidermal growth factor inhibitor 4-(3-chloro-benzyl)- 6,7-dimethoxy-quinazoline (tyrphostin), we investigated the kinetics of p120-catenin and periplakin in the human buccal mucosa squamous cancer cell line BICR 10 treated with 3 nM tyrphostin. Growth of BICR 10 cells was inhibited by treatment with tyrphostin. Although changes were not observed in the expression of EGFR and p120-catenin, expression of Akt, Src and periplakin in BICR 10 treated with 3 nM tyrphostin tended to decrease. In addition, phosphorylation of EGFR, Akt and Src was inhibited by treatment with tyrphostin. On immunocytochemical staining, immunoreactions with phosphorylated EGFR, phosphorylated Akt and phosphorylated p120-catenin were weak in BICR 10 treated with tyrphostin. There was a slight immunocy to chemical reaction to periplakin in BICR 10 cells induced by tyrphostin. In conclusion, the decrease in phosphorylation in EGFR and p120-catenin by tyrphostin, following the decrease in Src or Akt phosphorylation, may inhibit expression of several growth factors associated with the proliferation and migration of cancer cells.展开更多
The physics behind the spectral energy distribution (SED) of blazars remains open. We assembled 36 blazars to tackle the factors that control their SED. Now, many blazar spectra have the "double hump" feature in t...The physics behind the spectral energy distribution (SED) of blazars remains open. We assembled 36 blazars to tackle the factors that control their SED. Now, many blazar spectra have the "double hump" feature in the radio and far-IR frequencies. For these a parameter, △, is created to characterize the behavior of the SED. We found a significant correlation between the broad-line luminosity (LBLR) and △. Because L BLR is an indicator of the accreting power of the source in blazars, we derived a linear correlation, △ oc M1/3.18, which suggests that the SED of blazars may depend on the accretion rate, like that of BL Lac objects. We also found a significant correlation between m and △ for a sample of 11 blazars (out of one of 36) with available black hole masses. This implies the Eddington accretion ratio may influence the shape of the SED of blazars.展开更多
As climate change intensifies,finding an ecological indicator to quickly and accurately reflect the impact on mountain ecosystems is necessary.The low treeline/timberline,highly sensitive to climate variability and ch...As climate change intensifies,finding an ecological indicator to quickly and accurately reflect the impact on mountain ecosystems is necessary.The low treeline/timberline,highly sensitive to climate variability and changes significantly within 5–10years,provides a new way to study the response to regional climate variability.This study explored the distribution and vertical displacement patterns of the low treeline in the Upper Minjiang River of China,using SPOT remote sensing images in 1999 and 2013and long-term positional observations.Using the Geodetector model,the study investigated the dominant climatic factors influencing the low treeline displacement.The results showed that the low treeline was located at 1700–3200 m elevation on sunny slopes(southeast,south,southwest,and west slopes)with slopes over 25°.From 1999 to 2013,the low treeline moved downward by 6 m from 2561±264m to 2555±265 m,along with a warm–humid climate tendency.The downward displacement was greater on slopes over 25°and shady slopes(-20 m and-10 m,respectively)than on slopes≤25°and sunny slopes.Additionally,the downward was greater in the warm and humid Zagunao River Basin(-15 m)compared to the arid valley center(-7 m)and the cold Heishui River Basin(-3 m).Meanwhile,the low treeline displacement correlated negatively with precipitation and relative humidity variations at the significance level of 0.05,with correlation coefficients of-0.572and-0.551,respectively.Variations in relative humidity and temperature significantly affected the spatial differentiation of low treeline displacement with influencing power of 0.246(p=0.036<0.05)and 0.183(p=0.032<0.05),respectively.Thus,the low treeline is a moisture-limited line,and its formation and variation are closely related to regional water–heat balance.The study clarifies the indicative value of the low treeline for climate variability in mountain areas and can provide references for ecological restoration in arid valleys.展开更多
We report on our research on the UV, optical line parameters and the infrared through UV to soft X-ray continuum parameters for a composite sample of narrow line and normal Seyfert 1 galaxies. The strong correlations ...We report on our research on the UV, optical line parameters and the infrared through UV to soft X-ray continuum parameters for a composite sample of narrow line and normal Seyfert 1 galaxies. The strong correlations among the line width of HB, optical line strength of Fell and the soft X-ray slope are confirmed. We found no correlations between the UV line parameters, the equivalent widths of Lya and CIV and their ratio on one hand and the optical line parameters on the other. The UV and X-ray luminosities strongly correlate with the line widths of HB and the Fe II/HB ratio. No significant correlation is found between the infrared-soft X-my continuum slope aix and the line width of HB.展开更多
We used multi-component profiles to model the HE and [O Ⅲ]λλ4959,5007 lines of SDSS J143030.22-001115.1, a narrow-line Seyfert 1 galaxy (NLS1) in a sample of 150 NLS1 candidates selected from the Sloan Digital Sk...We used multi-component profiles to model the HE and [O Ⅲ]λλ4959,5007 lines of SDSS J143030.22-001115.1, a narrow-line Seyfert 1 galaxy (NLS1) in a sample of 150 NLS1 candidates selected from the Sloan Digital Sky Survey (SDSS), Early Data Release (EDR). After subtracting the HE contribution from narrow line regions (NLRs), we found that its full width half maximum (FWHM) of broad H/3 line is nearly 2900km s^-1, significantly larger than the customarily adopted criterion of 2000 km s^-1. With its weak Fe II multiples, we believe that SDSS J143030.22-001115.1 should not be classified as a genuine NLS1. When we calculate the virial black hole masses of NLSls, we should use the Hβ linewidth after subtracting the NLR component.展开更多
Long-term spectroscopic monitoring campaigns on active galactic nuclei(AGNs)provide a wealth of information about its interior structure and kinematics.However,a number of the observations suffer from the contaminatio...Long-term spectroscopic monitoring campaigns on active galactic nuclei(AGNs)provide a wealth of information about its interior structure and kinematics.However,a number of the observations suffer from the contamination of second-order spectra(SOS)which will introduce some undesirable uncertainties at the red side of the spectra.In this paper,we test the effect of SOS and propose a method to correct it in the time domain spectroscopic data using the simultaneously observed comparison stars.Based on the reverberation mapping(RM)data of NGC 5548 in2019,one of the most intensively monitored AGNs by the Lijiang 2.4 m telescope,we find that the scientific object,comparison star,and spectrophotometric standard star can jointly introduce up to~30%SOS for Grism 14.This irregular but smooth SOS significantly affects the flux density and profile of the emission line,while having little effect on the light curve.After applying our method to each spectrum,we find that the SOS can be corrected effectively.The deviation between corrected and intrinsic spectra is~2%,and the impact of SOS on time lag is very minor.This method makes it possible to obtain the HαRM measurements from archival data provided that the spectral shape of the AGN under investigation does not have a large change.展开更多
文摘Land subsidence is a major factor that affects metro line (ML) stability. In this study, an improved multi- temporal interferometric synthetic aperture radar (InSAR) (MTI) method to detect land subsidence near MLs is presented. In particular, our multi-temporal InSAR method provides surface subsidence measurements with high observation density. The MTI method tracks both point-like targets and distributed targets with temporal radar back- scattering steadiness. First, subsidence rates at the point targets with low-amplitude dispersion index (ADI) values are extracted by applying a least-squared estimator on an optimized freely connected network. Second, to reduce error propagation, the pixels with high-ADI values are classified into several groups according to ADI intervals and processed using a Pearson correlation coefficient and hierarchical analysis strategy to obtain the distributed targets. Then, nonlinear subsidence components at all point-like and distributed targets are estimated using phase unwrapping and spatiotemporal filtering on the phase residuals. The proposed MTI method was applied to detect land subsidence near MLs of No. 1 and 3 in the Baoshan district of Shanghai using 18 TerraSAR-X images acquired between April 21, 2008 and October 30, 2010. The results show that the mean subsidence rates of the stations distributed along the two MLs are -12.9 and -14.0 ram/year. Furthermore, three subsidence funnels near the MLs are discovered through the hierarchical analysis. The testing results demonstrate the satisfactory capacity of the proposed MTI method in providing detailed subsidence information near MLs.
基金Supported by Key Disciplines Group Construetion Project of Pudong Health Bureau of Shanghai(Grant No.PWZxk2010-12)
文摘Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant reiroviral vector-mediated gene transfer method.The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide(MTT)and cell colony formation assays.eyclnD1,p21^(cipt)and p27^(kipt)mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR.cyclinD1,p21^(cipt),p27^(kipt),AKT,p-AKT,FOXOt and p-FOX01 expressions in the transfected Tca8113 were detected by western blot analysis.In addition,miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.Results:Quantitative PCR showed that mitt-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue.MTT and cell colony formation assays showed that after miR-25-3p overexpression,the proliferation of transfected Tca8113 was obviously attenuated.Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression.p21^(cipt)and p27^(kipt)expressions were upregulated,while cyclinD1,AKT,FOXO1 expressions were downregulated,and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.Conclusions:MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression,playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.
基金Supported by the National Natural Science Foundation of China(Grant Nos. 10973012 and 11033007)the National Basic Research Program of China (973 program Grant Nos.2007CB815405 and 2009CB824800)
文摘We report the discovery of a broad absorption line (BAL) of - 10^4 km s^-1 in width in the previously known BL Lac object PKS 0138-097, which we tentatively identified as an Mg II BAL. This is the first detection of a BAL, which is sometimes seen in powerful quasars with high accretion rates, in a BL Lac object. The BAL was clearly detected in its spectra spanning two epochs at a high luminosity state taken in the Sloan Digital Sky Survey (SDSS), while it disappeared in three SDSS spectra taken at a low luminosity state. The BAL and its variability pattern were also found in its historical multi-epoch spectra in the literature, but have been overlooked previously. In its high resolution radio maps, PKS 0138-097 shows a core plus a one- sided parsec-scale jet. The BAL variability can be interpreted as follows: The optical emission is dominated by the core in a high state and by the jet in a low state and the BAL material is located between the core and jet so that the BAL appears only when the core is shining. Our discovery suggests that outflows may also be produced in active galactic nuclei at a low accreting state.
基金Supported by the National Natural Science Foundation of China.
文摘We analyze the spectral line broadening of those magneto-sensitive lines in solar magnetized atmospheres. The broadening at the line wings is due to the increase of the effective width of energy levels involved in Zeeman splitting,and the broadening at the line core also originated in Zeeman splitting under the condition that the Zeeman components are mixed. Therefore, the magneto-induced or Zeeman broadening take effects on the whole line. The observed Stokes parameter data in a sunspot and outside it acquired by Solar Stokes Spectrum Telescope (S^3T) are analyzed for the demonstration of this mechanism, and the Zeeman broadening rates are calculated for Fe16302.5 under some assumptions.Our result shows that the broadening is increased as the magnetic field strength becomes stronger, but the rate of increase at the line core is decreased as the field strength increases, while the rate at the wing does not show such an obvious regularity. The broadening is more effective in the line core than in the wings.
文摘The line profiles of Ha in a limb flare on 1998 November 11 appear to be unusually broadened. It is considered that macro-turbulence (or macroscopic mass motions) may be one of the main causes. We use an inversion technique to extract the probability distribution of the line-of-sight velocity in the flare. There exist some differences between the velocity distributions deduced from Ha and from Ca II λ8542, which may be because the two lines depend differently on the temperature and velocity. Since the loop density is high, we obtain a rather short cooling time (several tens of seconds) from the hot X-ray loops to the cool loops visible in Ha. Possible origins of the large scale motions are discussed.
文摘For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the effect of gastric carcinoma cell differentiation by chemical inducers.Under light microscope, MGc80-3 cells, after treated with 1 mM dBcAMP, tended to be flat and disperse, and their volume gradually enlarged, with their uncleus relatively smaller and their shape rather regular. Morphological changes, like norma differentiated epithelial cells, were observed. The cells attached firmly, grew slowly, their growth curve showed inhibitory rate amounted to 52.87%, and cellular division exponent displayed their peak value 1.5 times less than that of MGc80-3 cells. It was clear that dBcAMP could effectively inhibit the multiplication activity of MGc80-3 cells. After dBcAMP treatment, remarkable changes of cell surface charges was indicated by cell electrophoresis, the ratio dropped to 3.043 from 3.988, and their re-tardant ratio reached up to 31.2%. cAMP content in cells after this treatment, detected by cAMP and cGMP radioimmunoassay, was enhanced by 2.42 times, and cAMP/cGMP ratio, by 1.73 times. Thus, cAMP level within MGc80-3 cells was raised obviously by dBcAMP. Heterotransplantation experiments showed that tuntorigenic rate of MGc80-5 cells (transplanted subcutaneously to BALB/c mice) amounted to 100%, and that of the cells after this treatment was only 5.6%. Their tumorigenic ability was extremely reduced.These results confirmed that dBcAMP was able to change malignant phenotypic characteristics of MGc80-3 cells and produce a reversed alteration: Thus, it has a remarkable inductive effect in differentiating gastric carcinoma cells. All these characteristics were also considered as the reference indexes in appraising reversed effect for the homologous cancer cells.
基金support of the RFBR grants(projects 15–02–06098,16–02–00761 and18–02–00660)support of the Russian Science Foundation grant(project 17–12–01256)
文摘In order to search for intensity fluctuations on the HCN(1-0) and HCO+(1-0) line pro- files, which could arise due to possible small-scale inhomogeneous structure, long-term observations of high-mass star-forming cores S140 and S199 were carried out. The data were processed by the Fourier filtering method. Line temperature fluctuations that exceed the noise level were detected. Assuming the cores consist of a large number of randomly moving small thermal fragments, the total number of frag- ments is - 4 × 106 for the region with linear size - 0.1 pc in S140 and - 106 for the region with linear size - 0.3 pc in S 199. Physical parameters of fragments in S 140 were obtained from detailed modeling of the HCN emission in the framework of the clumpy cloud model.
文摘A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5' AMPase, ACP etc.) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on day 5 with mitotic index reaching 1.8% on day 3. This cell line was a hyperdiploid with karyotype of 45 or 45, -2X, tril2, tri17, +M1-5. Cell agglutination was observed when treated with ConA (≥7 fig, ml). Spontaneous agglutination might also take place without adding any ConA. After 5×106 cells of Ca 761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutan eous, intrafoot-pad or intraperitoneal), the transplan lability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca 761-86 may be of value for practical purposes.
基金This project was supported by a grant from the Teaching and Research Award Program for Outstanding Young Teacher in Higher Education Institution of Ministry of Education of China.
文摘In order to study the effect of 5, 6-Dichloro-l-13-D-ribofuranosyl-benzimidazole (DRB) on the biological characteristics of human laryngeal carcinoma Hep-2 cell line in vitro, Hep-2 cells cultured in vitro were treated with different concentrations of DRB. Changes in cell proliferation, apoptotic rate and invasiveness were detected by MTT assay, flow cytometry (FCM) and matrigel in vitro invasion assay, respectively. It was found that DRB inhibited the proliferation of Hep-2 cells in a dose- and time-dependent manner. After being treated with 0, 10, 20, 40, 80 μmmol/L DRB for 24 h, the apoptotic rate in Hep-2 cells was (0.68±0.19)%, (1.95±0.12)%, (8.51±0.26)%, (11.26±0.17)% and (14.99±0.32)%, respectively. The matrigel in vitro invasion assay revealed that DRB began to inhibit the invasion of Hep-2 cells at the concentration of 5 μmmol/L, and with the increase of DRB concentration, the inhibitory effect was enhanced. It was suggested that DRB could influence the essential biological characteristics of Hep-2 cells, inhibit Hep-2 cells proliferation, reduce invasive ability and induce apoptosis of Hep-2 cells.
基金supported by the SOC program (CHINARE 2012-02-03)the National Natural Science Foundation of China (NSFC 11473025, 11033007, 11421303, 11503022 and 11473305)+2 种基金the National Basic Research Program of China (the 973 Program 2013CB834905)Strategic Priority Research Program “The Emergence of Cosmological Structures” (XDB 09030200)provided by the Alfred P. Sloan Foundation, Participating Institutions, National Science Foundation, U.S. Department of Energy, NASA, Japanese Monbukagakusho, Max Planck Society,and Higher Education Funding Council for England
文摘Emission lines from the broad emission line region (BELR) and the narrow emission line region (NELR) of active galactic nuclei (AGNs) have been extensively studied. However, emission lines are rarely detected between these two regions. We present a detailed analysis of quasar SDSS J232444.80-094600.3 (SDSS J2324-0946), which is remarkable for its strong intermediate-width emission lines (IELs) with FWHM ≈ 1800 km s^-1. The IEL component is present in different emission lines, including the permitted lines Lyαλ1216, CⅣ λ1549, semiforbidden line C Ⅲ] λ1909, and forbidden lines [OⅢ] ss4959, 5007. With the aid of photo-ionization models, we found that the IELs are produced by gas with a hydrogen density of nH - 10^6.2 -- 10^6.3 cm^-3, a distance from the central ionizing source of R - 35 - 50 pc, a covering factor of - 6%, and a dust-to-gas ratio of ≤ 4% that of the SMC. We suggest that the strong IELs of this quasar are produced by nearly dust-free and intermediate-density gas located at the skin of the dusty torus. Such strong IELs, which serve as a useful diagnostic, can provide an avenue to study the properties of gas between the BELR and the NELR.
基金Supported by the National Natural Science Foundation of China.
文摘We present an exercise that intends to establish a relationship between the strength of nebular emission lines and optical stellar features in the spectrum of a galaxy. After accurately subtracting the stellar continuum and the underlying stellar absorption, we made reliable measurements of the emission lines of all the galaxies in the Sloan Digital Sky Survey Data Release 2 (SDSS DR2). More than 4000 star-forming galaxies with high S/N ratio of both the stellar spectrum and the emission lines are selected. These galaxy spectra are fitted with the 10 PCs of Yip et al., after all the emission line regions have been filtered out. We find that the flux of hydrogen Balmer emission lines, Ha and Hβ can be well recovered from the PCs, while the metal lines are not well reproduced. The fluxes of Ha and Hβ measured from the PC-reconstructed spectra and from the observed spectra agree well with an rms scatter of only - 0.1 dex. This result suggests that, with moderate spectral resolution and S/N ratio, the optical stellar spectrum of a galaxy can serve as an indicator of star formation rate.
基金support by the Youth Innovation Promotion Association of CASThe Submillimeter Array is a joint project between the Smithsonian Astrophysical Observatory and the Academia Sinica Institute of Astronomy and Astrophysics and is funded by the Smithsonian Institution and Academia Sinica
文摘Line surveys of complex molecules with millimeter and sub-millimeter telescopes are important for probing the physical and chemical environments of massive star forming regions(MSFRs).We present a molecular line survey with the Submillimeter Array(SMA) in the frequency ranges of 220.3–222.3 GHz and 230.3–232.3 GHz toward G10.6-0.4, the brightest star forming core in the W31 complex. Ninety-nine transitions from 22 molecular species and their isotopologues are identified. The moment 0 images of typical molecules show a compact core which is concentrated at the continuum peak position. Based on the local thermodynamic equilibrium assumption, the molecular line data are modeled. The rotational temperatures of those molecular species range from 96 to 178 K and their column densities range from 2.0×1014to 3.7×1017cm-2. The observational data suggest that all complex molecules are located in a warm environment. Chemical environments of the molecules are discussed. We compared molecular abundances and gas temperatures in G10.6-0.4 with those in other MSFRs, and found that gas temperatures and fractional abundances of specific molecules in G10.6-0.4 are similar to the typical MSFR W51 North, suggesting that there are similar physical and chemical environments in these two MSFRs.
文摘In order to detect molecular markers for the epidermal growth factor inhibitor 4-(3-chloro-benzyl)- 6,7-dimethoxy-quinazoline (tyrphostin), we investigated the kinetics of p120-catenin and periplakin in the human buccal mucosa squamous cancer cell line BICR 10 treated with 3 nM tyrphostin. Growth of BICR 10 cells was inhibited by treatment with tyrphostin. Although changes were not observed in the expression of EGFR and p120-catenin, expression of Akt, Src and periplakin in BICR 10 treated with 3 nM tyrphostin tended to decrease. In addition, phosphorylation of EGFR, Akt and Src was inhibited by treatment with tyrphostin. On immunocytochemical staining, immunoreactions with phosphorylated EGFR, phosphorylated Akt and phosphorylated p120-catenin were weak in BICR 10 treated with tyrphostin. There was a slight immunocy to chemical reaction to periplakin in BICR 10 cells induced by tyrphostin. In conclusion, the decrease in phosphorylation in EGFR and p120-catenin by tyrphostin, following the decrease in Src or Akt phosphorylation, may inhibit expression of several growth factors associated with the proliferation and migration of cancer cells.
基金This work was supported by the Natural Science Foundation of Yunnan Province of China.
文摘The physics behind the spectral energy distribution (SED) of blazars remains open. We assembled 36 blazars to tackle the factors that control their SED. Now, many blazar spectra have the "double hump" feature in the radio and far-IR frequencies. For these a parameter, △, is created to characterize the behavior of the SED. We found a significant correlation between the broad-line luminosity (LBLR) and △. Because L BLR is an indicator of the accreting power of the source in blazars, we derived a linear correlation, △ oc M1/3.18, which suggests that the SED of blazars may depend on the accretion rate, like that of BL Lac objects. We also found a significant correlation between m and △ for a sample of 11 blazars (out of one of 36) with available black hole masses. This implies the Eddington accretion ratio may influence the shape of the SED of blazars.
基金the Natural Science Foundation of Southwest University of Science and Technology(18zx7117)the National Science and Technology Support Program of China(2015BAC05B05-01)。
文摘As climate change intensifies,finding an ecological indicator to quickly and accurately reflect the impact on mountain ecosystems is necessary.The low treeline/timberline,highly sensitive to climate variability and changes significantly within 5–10years,provides a new way to study the response to regional climate variability.This study explored the distribution and vertical displacement patterns of the low treeline in the Upper Minjiang River of China,using SPOT remote sensing images in 1999 and 2013and long-term positional observations.Using the Geodetector model,the study investigated the dominant climatic factors influencing the low treeline displacement.The results showed that the low treeline was located at 1700–3200 m elevation on sunny slopes(southeast,south,southwest,and west slopes)with slopes over 25°.From 1999 to 2013,the low treeline moved downward by 6 m from 2561±264m to 2555±265 m,along with a warm–humid climate tendency.The downward displacement was greater on slopes over 25°and shady slopes(-20 m and-10 m,respectively)than on slopes≤25°and sunny slopes.Additionally,the downward was greater in the warm and humid Zagunao River Basin(-15 m)compared to the arid valley center(-7 m)and the cold Heishui River Basin(-3 m).Meanwhile,the low treeline displacement correlated negatively with precipitation and relative humidity variations at the significance level of 0.05,with correlation coefficients of-0.572and-0.551,respectively.Variations in relative humidity and temperature significantly affected the spatial differentiation of low treeline displacement with influencing power of 0.246(p=0.036<0.05)and 0.183(p=0.032<0.05),respectively.Thus,the low treeline is a moisture-limited line,and its formation and variation are closely related to regional water–heat balance.The study clarifies the indicative value of the low treeline for climate variability in mountain areas and can provide references for ecological restoration in arid valleys.
基金the National Natural Science Foundationof China and Chinese Panden Project.
文摘We report on our research on the UV, optical line parameters and the infrared through UV to soft X-ray continuum parameters for a composite sample of narrow line and normal Seyfert 1 galaxies. The strong correlations among the line width of HB, optical line strength of Fell and the soft X-ray slope are confirmed. We found no correlations between the UV line parameters, the equivalent widths of Lya and CIV and their ratio on one hand and the optical line parameters on the other. The UV and X-ray luminosities strongly correlate with the line widths of HB and the Fe II/HB ratio. No significant correlation is found between the infrared-soft X-my continuum slope aix and the line width of HB.
基金Supported by the National Natural Science Foundation of China.
文摘We used multi-component profiles to model the HE and [O Ⅲ]λλ4959,5007 lines of SDSS J143030.22-001115.1, a narrow-line Seyfert 1 galaxy (NLS1) in a sample of 150 NLS1 candidates selected from the Sloan Digital Sky Survey (SDSS), Early Data Release (EDR). After subtracting the HE contribution from narrow line regions (NLRs), we found that its full width half maximum (FWHM) of broad H/3 line is nearly 2900km s^-1, significantly larger than the customarily adopted criterion of 2000 km s^-1. With its weak Fe II multiples, we believe that SDSS J143030.22-001115.1 should not be classified as a genuine NLS1. When we calculate the virial black hole masses of NLSls, we should use the Hβ linewidth after subtracting the NLR component.
基金funded by the National Key R&D Program of China with No.2021YFA1600404the National Natural Science Foundation of China(NSFC+6 种基金grant Nos.11991051,12303022,12373018,12203096,12103041,12073068)Yunnan Fundamental Research Projects(grant Nos.202301AT070339,202301AT070358)Yunnan Postdoctoral Foundation Funding Project,the Yunnan Province Foundation(202001AT070069)the Youth Innovation Promotion Association of the Chinese Academy of Sciences(2022058)the Topnotch Young Talents Program of Yunnan Province,Special Research Assistant Funding Project of Chinese Academy of Sciencesthe science research grants from the China Manned Space Project with No.CMS-CSST-2021-A06Funding for the telescope has been provided by the Chinese Academy of Sciences and the People’s Government of Yunnan Province。
文摘Long-term spectroscopic monitoring campaigns on active galactic nuclei(AGNs)provide a wealth of information about its interior structure and kinematics.However,a number of the observations suffer from the contamination of second-order spectra(SOS)which will introduce some undesirable uncertainties at the red side of the spectra.In this paper,we test the effect of SOS and propose a method to correct it in the time domain spectroscopic data using the simultaneously observed comparison stars.Based on the reverberation mapping(RM)data of NGC 5548 in2019,one of the most intensively monitored AGNs by the Lijiang 2.4 m telescope,we find that the scientific object,comparison star,and spectrophotometric standard star can jointly introduce up to~30%SOS for Grism 14.This irregular but smooth SOS significantly affects the flux density and profile of the emission line,while having little effect on the light curve.After applying our method to each spectrum,we find that the SOS can be corrected effectively.The deviation between corrected and intrinsic spectra is~2%,and the impact of SOS on time lag is very minor.This method makes it possible to obtain the HαRM measurements from archival data provided that the spectral shape of the AGN under investigation does not have a large change.