Effect of High Temperature on Outcrossing Characteristics at the Fertility Sensitive Period of Photo-thermo Sensitive Genic Male Sterile (PTGMS) Rice Lines

Four sterile lines （Peiai64S, Y58S, Guangzhan 63-2S and H638S） and the restoring line R527 were used as materials. Five temperature gradients （24, 27, 30, 33 and 36 ℃ in artificial climate chamber） and the natural temperature （as control） were treated to the four sterile lines for 6 d in the fertility sensitive period of heading to flowering stage, respectively, to study the effects of temperature on physiological biochemical characteristics of young panicles and outcrossing characteristics. The results showed that the percentages of exerted stigma of Peiai 64S and Y58S were the highest at 27 ℃, which were 86.81% and 86.06%, respectively, while the percentages of exerted stigma of Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 76.24% and 81.76%, respectively; the stigma viability of Peiai 64S, Y58S, Guangzhan 63-2S and H638S were the best at 24 ℃, which were 1.96, 2.12, 1.74 and 1.94, respectively; the outcrossing rates of Peiai 64S, Guangzhan 63-2S and H638S were the highest at 24 ℃, which were 58.87%, 54.22% and 50.50%, respectively, while the outcrossing rate of Y58S was the highest at 27 ℃, which was 58.96%; and the contents of peroxidase （POD） of the four sterile lines increased significantly at 33 ℃ compared with the control, while the contents of malondialdehyde （MDA） and proline increased significantly at 36 ℃ compared with the control. There were differences in temperature sensitivity between the male sterile lines, and the 24 ℃ treatment during the sensitive period was the best for the fertility sensitive period of Peiai 64S, while 27 ℃ was the best temperature for Y58S, Gangzhan 63-2S and H638S.

On-Line Suppression of Periodic Noises in Evoked Potentials

Under clinical conditions,the major components of noises in human evoked potentialsare periodic impulses,such as power interferences, system oscillations. Reducing their effects by analogfilter is inevitably associated with the distortions of the signal of interest.In this paper,a new approachto suppress periodic noises is presented using time-domain operators which are applied on the individualdigitized responses before averaging.A new algorithm for real time processing is developed.The effectiveness of the algorithm in dealing with simulated as well as actual evoked responses is demonstrated.The results show that this algorithm has good performance in periodic noise suppression and has zerophase shift, thus it is very suitable to be implemented on biomedical instrumentations used inclinical setups.

Variations of Frost-free Period and Its Impact on Grain Yields in Henan Province during 1961-2013

The aim of this study was to investigate the responses of frost dates to global warming and its influences on grain yields. In this study, based on the frost date series defined by daily minimum ground temperature, the spatial and temporal characteristics of first frost date （FFD）, last frost date （LFD） and frost-free period （FFP） were analyzed. The impact of extending FFP on major crop yields was also studied. The results were as follows： FFD showed a significantly delaying trend of 2.2 d/10 y, and LFD presented an advancing trend of 2.4 d/10 y. FFP extended at a rate of 4.5 d/10 y due to the later FFD and earlier LFD. The most obvious trend of FFD was in westem Henan, while the most significant trend of LFD and FFP oc- curred in south central parts of the study area. However, in eestem region, the trends of FFD, LFD and FFP were not so obvious. Major crop yield showed a sig- nificant correlation with frost-free period for Henan during 1961-2013. The yields of grain, rice, wheat, and maize increased by 79.5, 90.0, 79.5 and 70.5 kg/hm2 with FFP extending by one day.

框架-核心筒结构外框刚度贡献的直接计算法与间接评估法

针对框架-核心筒结构体系抗震设计,提出2种新的评估外框刚度贡献的方法:直接计算法和间接评估法。前者通过施加侧向荷载直接计算核心筒抗侧刚度和整体结构的抗侧刚度,通过差值比例得到外框刚度贡献率;后者则通过一个新的结构指标“二阶平动周期比”来间接反映外框刚度的贡献率,通过理想案例研究了影响“二阶平动周期比”数值的相关因素和基本规律。选择8个实际工程案例,分别采用2种方法对其外框刚度贡献进行了评价,并对2种方法评价结果的相关性进行研究。对比显示,2种方法得到的结果基本一致,能够相互印证。在此基础上,将框架-核心筒结构外框刚度贡献分为较低、中等和较大3个区间,并分别给出对应的指标数值范围。对2个实际工程采用不同种方法进行外框刚度评估,并对其抗震性能进行验证,结果表明,采用文中方法能够更加合理评价框架-核心筒外框刚度贡献,形成更加科学的抗震设计。

Periodic oscillation and fine structure of wedge-induced oblique detonation waves

An oblique detonation wave for a Mach 7 inlet flow over a long enough wedge of 30 turning angle is simulated numerically using Euler equation and one-step rection model.The fifth-order WENO scheme is adopted to capture the shock wave.The numerical results show that with the compression of the wedge wall the detonation wave front structure is divided into three sections：the ZND model-like strcuture,single-sided triple point structure and dual-headed triple point strucuture.The first structure is the smooth straight,and the second has the characteristic of the triple points propagating dowanstream only with the same velocity,while the dual-headed triple point structure is very complicated.The detonation waves facing upstream and downstream propagate with different velocities,in which the periodic collisions of the triple points cause the oscillation of the detonation wave front.This oscillation process has temporal and spatial periodicity.In addition,the triple point trace are recorded to obtain different cell structures in three sections.

ANTIPLANE PROBLEMS OF PERIODICAL RIGID LINE INCLUSIONS BETWEEN DISSIMILAR ANISOTROPIC MATERIALS

The antiplane shear problems of periodical rigid line inclusions between dissimilar anisotropic materials are dealt with. By using the complex variable method, the closed form solutions are obtained. The stress distribution in the immediate vicinity of the rigid line is examined. The corresponding formulation between dissimilar isotropic materials and in homogeneous anisotropic medium can be derived from the special cases of those in the present paper, and the limit conditions are in agreement with the previously known results.

Periodic Packing Mode for Trickle-Bed Reactors: Experiments and Modeling

A periodic packing mode of trickle-bed reactor (TBR) for the gas limited reaction was proposed. Hy-drogenation of 2-ethylanthraquinone over Pd/Al2O3 in a laboratory-scale TBR was taken as a test reaction for determining whether the periodic packing mode is advantageous. The effects of operating conditions and packing type on TBR performance were experimentally examined to demonstrate the cause-effect relationships. A mathe-matic model of TBR considering axial dispersion and fractional wetting was developed to quantitatively illuminate the reason of performance enhancement.

Detecting land subsidence near metro lines in the Baoshan district of Shanghai with multi-temporal interferometric synthetic aperture radar

Land subsidence is a major factor that affects metro line （ML） stability. In this study, an improved multi- temporal interferometric synthetic aperture radar （InSAR） （MTI） method to detect land subsidence near MLs is presented. In particular, our multi-temporal InSAR method provides surface subsidence measurements with high observation density. The MTI method tracks both point-like targets and distributed targets with temporal radar back- scattering steadiness. First, subsidence rates at the point targets with low-amplitude dispersion index （ADI） values are extracted by applying a least-squared estimator on an optimized freely connected network. Second, to reduce error propagation, the pixels with high-ADI values are classified into several groups according to ADI intervals and processed using a Pearson correlation coefficient and hierarchical analysis strategy to obtain the distributed targets. Then, nonlinear subsidence components at all point-like and distributed targets are estimated using phase unwrapping and spatiotemporal filtering on the phase residuals. The proposed MTI method was applied to detect land subsidence near MLs of No. 1 and 3 in the Baoshan district of Shanghai using 18 TerraSAR-X images acquired between April 21, 2008 and October 30, 2010. The results show that the mean subsidence rates of the stations distributed along the two MLs are -12.9 and -14.0 ram/year. Furthermore, three subsidence funnels near the MLs are discovered through the hierarchical analysis. The testing results demonstrate the satisfactory capacity of the proposed MTI method in providing detailed subsidence information near MLs.

MiR-25-3p attenuates the proliferation of tongue squamous cell carcinoma cell line Tca8113

Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant reiroviral vector-mediated gene transfer method.The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide(MTT)and cell colony formation assays.eyclnD1,p21^(cipt)and p27^(kipt)mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR.cyclinD1,p21^(cipt),p27^(kipt),AKT,p-AKT,FOXOt and p-FOX01 expressions in the transfected Tca8113 were detected by western blot analysis.In addition,miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.Results:Quantitative PCR showed that mitt-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue.MTT and cell colony formation assays showed that after miR-25-3p overexpression,the proliferation of transfected Tca8113 was obviously attenuated.Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression.p21^(cipt)and p27^(kipt)expressions were upregulated,while cyclinD1,AKT,FOXO1 expressions were downregulated,and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.Conclusions:MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression,playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.

Distortionless Lossy Transmission Lines Terminated by in Series Connected <i>RCL</i>-Loads

The paper deals with a lossy transmission line terminated at both ends by non-linear RCL elements. The mixed problem for the hyperbolic system, describing the transmission line, to an initial value problem for a neutral equation is reduced. Sufficient conditions for the existence and uniqueness of periodic regimes are formulated. The proof is based on the finding out of suitable operator whose fixed point is a periodic solution of the neutral equation. The method has a good rate of convergence of the successive approximations even for high frequencies.

Discovery of a variable broad absorption line in the BL Lac object PKS B0138-097

We report the discovery of a broad absorption line （BAL） of - 10^4 km s^-1 in width in the previously known BL Lac object PKS 0138-097, which we tentatively identified as an Mg II BAL. This is the first detection of a BAL, which is sometimes seen in powerful quasars with high accretion rates, in a BL Lac object. The BAL was clearly detected in its spectra spanning two epochs at a high luminosity state taken in the Sloan Digital Sky Survey （SDSS）, while it disappeared in three SDSS spectra taken at a low luminosity state. The BAL and its variability pattern were also found in its historical multi-epoch spectra in the literature, but have been overlooked previously. In its high resolution radio maps, PKS 0138-097 shows a core plus a one- sided parsec-scale jet. The BAL variability can be interpreted as follows： The optical emission is dominated by the core in a high state and by the jet in a low state and the BAL material is located between the core and jet so that the BAL appears only when the core is shining. Our discovery suggests that outflows may also be produced in active galactic nuclei at a low accreting state.

Magneto-induced Line Broadening of Magneto-sensitive Lines in Solar Magnetized Atmospheres

We analyze the spectral line broadening of those magneto-sensitive lines in solar magnetized atmospheres. The broadening at the line wings is due to the increase of the effective width of energy levels involved in Zeeman splitting,and the broadening at the line core also originated in Zeeman splitting under the condition that the Zeeman components are mixed. Therefore, the magneto-induced or Zeeman broadening take effects on the whole line. The observed Stokes parameter data in a sunspot and outside it acquired by Solar Stokes Spectrum Telescope （S^3T） are analyzed for the demonstration of this mechanism, and the Zeeman broadening rates are calculated for Fe16302.5 under some assumptions.Our result shows that the broadening is increased as the magnetic field strength becomes stronger, but the rate of increase at the line core is decreased as the field strength increases, while the rate at the wing does not show such an obvious regularity. The broadening is more effective in the line core than in the wings.

Line Broadening in a Limb Flare: Derivation of Macro-turbulent Velocity Fields

The line profiles of Ha in a limb flare on 1998 November 11 appear to be unusually broadened. It is considered that macro-turbulence (or macroscopic mass motions) may be one of the main causes. We use an inversion technique to extract the probability distribution of the line-of-sight velocity in the flare. There exist some differences between the velocity distributions deduced from Ha and from Ca II λ8542, which may be because the two lines depend differently on the temperature and velocity. Since the loop density is high, we obtain a rather short cooling time (several tens of seconds) from the hot X-ray loops to the cool loops visible in Ha. Possible origins of the large scale motions are discussed.

THE DIFFERENTIATION OF HUMAN GASTRIC ADENOCAR-CINOMA CELL LINE MGc80-3 INDUCED BY DIBUTYRYL cAMP IN VITRO

For providing some experimental basis in establishing malignant phenotypic reversed indexes of gastric carcinoma cells, human gastric adenocar-cinoma cell line MGc80-3 was induced by dBcAMP in vitro to appraise the effect of gastric carcinoma cell differentiation by chemical inducers.Under light microscope, MGc80-3 cells, after treated with 1 mM dBcAMP, tended to be flat and disperse, and their volume gradually enlarged, with their uncleus relatively smaller and their shape rather regular. Morphological changes, like norma differentiated epithelial cells, were observed. The cells attached firmly, grew slowly, their growth curve showed inhibitory rate amounted to 52.87%, and cellular division exponent displayed their peak value 1.5 times less than that of MGc80-3 cells. It was clear that dBcAMP could effectively inhibit the multiplication activity of MGc80-3 cells. After dBcAMP treatment, remarkable changes of cell surface charges was indicated by cell electrophoresis, the ratio dropped to 3.043 from 3.988, and their re-tardant ratio reached up to 31.2%. cAMP content in cells after this treatment, detected by cAMP and cGMP radioimmunoassay, was enhanced by 2.42 times, and cAMP/cGMP ratio, by 1.73 times. Thus, cAMP level within MGc80-3 cells was raised obviously by dBcAMP. Heterotransplantation experiments showed that tuntorigenic rate of MGc80-5 cells (transplanted subcutaneously to BALB/c mice) amounted to 100%, and that of the cells after this treatment was only 5.6%. Their tumorigenic ability was extremely reduced.These results confirmed that dBcAMP was able to change malignant phenotypic characteristics of MGc80-3 cells and produce a reversed alteration: Thus, it has a remarkable inductive effect in differentiating gastric carcinoma cells. All these characteristics were also considered as the reference indexes in appraising reversed effect for the homologous cancer cells.

ANALYSIS OF OPEN MICROSTRIP STRUCTURES BY USING DIAKOPTIC METHOD OF LINES COMBINED WITH PERIODIC BOUNDARY CONDITIONS

This paper presents the analysis of open microstrip structures by using diakoptic method of lines (ML) combined with periodic boundary conditions (PBC). The parameters of microstrip patch are obtained from patch current excited by plane wave. Impedance matrix elements are computed by using fast Fourier transform(FFT), and reduced equation is solved by using diakoptic technique. Consequently, the computing time is reduced significantly. The convergence property of simulating open structure by using PBC and the comparison of the computer time between using PBC and usual absorbing boundary condition (ABC) show the validity of the method proposed in this paper. Finally, the resonant frequency of a microstrip patch is computed. The numerical results obtained are in good agreement with those published.

A search for evidence of small-scale inhomogeneities in dense cores from line profile analysis

In order to search for intensity fluctuations on the HCN（1-0） and HCO＋（1-0） line pro- files, which could arise due to possible small-scale inhomogeneous structure, long-term observations of high-mass star-forming cores S140 and S199 were carried out. The data were processed by the Fourier filtering method. Line temperature fluctuations that exceed the noise level were detected. Assuming the cores consist of a large number of randomly moving small thermal fragments, the total number of frag- ments is - 4 × 106 for the region with linear size - 0.1 pc in S140 and - 106 for the region with linear size - 0.3 pc in S 199. Physical parameters of fragments in S 140 were obtained from detailed modeling of the HCN emission in the framework of the clumpy cloud model.

ESTABLISHMENT OF A MAMMARY CANCER CELL LINE Ca 761-86 AND ITS BIOLOGICAL CHARACTERISTICS IN INBRED 615 MICE

A cell line designated as Ca 761-86 has been established from the solid mouse mammary cancer (Ca 761) by suspension culture. It has been passaged for more than 212 generations. Moderate round cells were predominant and most of them were mononuclear. Some characteristics of malignant cells and A-type viral-like particles were observed by electron microscopy. The results of cytochemical studies (DNA, RNA, SDH, 5' AMPase, ACP etc.) were comparable to the ultramicroscopic results. It multiplied approximately 27.4 fold on day 5 with mitotic index reaching 1.8% on day 3. This cell line was a hyperdiploid with karyotype of 45 or 45, -2X, tril2, tri17, +M1-5. Cell agglutination was observed when treated with ConA (≥7 fig, ml). Spontaneous agglutination might also take place without adding any ConA. After 5×106 cells of Ca 761-86 suspension were transplanted into the normal inbred 615 mice by different ways (subcutan eous, intrafoot-pad or intraperitoneal), the transplan lability rate reached 100%. Spontaneous remission was never observed and its metastatic ability reserved. PPLO were not detected. Ca 761-86 may be of value for practical purposes.

Strong optical and UV intermediate-width emission lines in the quasar SDSS J232444.80–094600.3:dust-free and intermediate-density gas at the skin of dusty torus?

Emission lines from the broad emission line region （BELR） and the narrow emission line region （NELR） of active galactic nuclei （AGNs） have been extensively studied. However, emission lines are rarely detected between these two regions. We present a detailed analysis of quasar SDSS J232444.80-094600.3 （SDSS J2324-0946）, which is remarkable for its strong intermediate-width emission lines （IELs） with FWHM ≈ 1800 km s^-1. The IEL component is present in different emission lines, including the permitted lines Lyαλ1216, CⅣ λ1549, semiforbidden line C Ⅲ] λ1909, and forbidden lines [OⅢ] ss4959, 5007. With the aid of photo-ionization models, we found that the IELs are produced by gas with a hydrogen density of nH - 10^6.2 -- 10^6.3 cm^-3, a distance from the central ionizing source of R - 35 - 50 pc, a covering factor of - 6%, and a dust-to-gas ratio of ≤ 4% that of the SMC. We suggest that the strong IELs of this quasar are produced by nearly dust-free and intermediate-density gas located at the skin of the dusty torus. Such strong IELs, which serve as a useful diagnostic, can provide an avenue to study the properties of gas between the BELR and the NELR.

Effect of DRB on the Biological Characteristics of Human Laryngeal Carcinoma Hep-2 Cell Line

In order to study the effect of 5, 6-Dichloro-l-13-D-ribofuranosyl-benzimidazole （DRB） on the biological characteristics of human laryngeal carcinoma Hep-2 cell line in vitro, Hep-2 cells cultured in vitro were treated with different concentrations of DRB. Changes in cell proliferation, apoptotic rate and invasiveness were detected by MTT assay, flow cytometry （FCM） and matrigel in vitro invasion assay, respectively. It was found that DRB inhibited the proliferation of Hep-2 cells in a dose- and time-dependent manner. After being treated with 0, 10, 20, 40, 80 μmmol/L DRB for 24 h, the apoptotic rate in Hep-2 cells was （0.68±0.19）%, （1.95±0.12）%, （8.51±0.26）%, （11.26±0.17）% and （14.99±0.32）%, respectively. The matrigel in vitro invasion assay revealed that DRB began to inhibit the invasion of Hep-2 cells at the concentration of 5 μmmol/L, and with the increase of DRB concentration, the inhibitory effect was enhanced. It was suggested that DRB could influence the essential biological characteristics of Hep-2 cells, inhibit Hep-2 cells proliferation, reduce invasive ability and induce apoptosis of Hep-2 cells.

Relation between Starlight and Nebular Emission Lines of Star-Forming Galaxies

We present an exercise that intends to establish a relationship between the strength of nebular emission lines and optical stellar features in the spectrum of a galaxy. After accurately subtracting the stellar continuum and the underlying stellar absorption, we made reliable measurements of the emission lines of all the galaxies in the Sloan Digital Sky Survey Data Release 2 (SDSS DR2). More than 4000 star-forming galaxies with high S/N ratio of both the stellar spectrum and the emission lines are selected. These galaxy spectra are fitted with the 10 PCs of Yip et al., after all the emission line regions have been filtered out. We find that the flux of hydrogen Balmer emission lines, Ha and Hβ can be well recovered from the PCs, while the metal lines are not well reproduced. The fluxes of Ha and Hβ measured from the PC-reconstructed spectra and from the observed spectra agree well with an rms scatter of only - 0.1 dex. This result suggests that, with moderate spectral resolution and S/N ratio, the optical stellar spectrum of a galaxy can serve as an indicator of star formation rate.