Effect of Enhanced UV-B Radiation on Seed Setting Rate and 1 000-grain Weight of Indica Hybrid Rice Restorer Lines

[Objective] This study was to investigate the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight of hybrid rice combinations. [Method] The seed setting rate and 1 000-grain weight of 10 new sterile indica restorer lines planted in pots under enhanced UV-B radiation and fluorescent lamps （control） were respectively measured, and the differences were compared. [Result] The enhanced UV-B radiation significantly reduced the seed setting rate of indica restorer lines, and the differences between that UV-B radiation treatment and control all reached extremely significant level. In addition, the enhanced UV-B radiation reduced the 1 000-grain weight of most indica restorer lines, and compared with that of control the difference achieved significant or very significant level. However, the effect of enhanced UV-B radiation on seed setting rate and 1 000-grain weight differed to different indica restorer lines, and the differences among restorer lines tested were significant or very significant, which indicated the possibility to screen antiUV-B radiation rice materials and combinations. Finally, the indica restorer lines 09R-14, Luhui 37 and 10R-7703 which were strongly resistant to UV-B radiation were screened out. [Conclusion] This study laid foundation for breeding hybrid rice varieties resistance to UV-B radiation.

On-Line Monitoring of Grinding Status Based on Rough Set Theory

This paper focuses on the new approach of on line monitoring of grinding burn and wheel wear based on the rough set theory. This method adopts the grinding chip flow thermal signal, and acquires identification rules by establishing sensitive characteristic parameters and constructing the knowledge system through continuum attribute discretization, attribute reduction and knowledge acquisition, and then monitors grinding burn and wheel wear in accordance with the acquired rules. The experiment results show that the new method is effective.

SETD5介导AKT1磷酸化调控结肠癌细胞的迁移和5-FU敏感性

目的:探讨含SET结构域蛋白5(SETD5)对结肠癌细胞增殖、迁移和对5-氟尿嘧啶(5-FU)药物敏感性的影响及机制。方法:常规培养结肠癌细胞,用Lipofectamine 2000将siSETD5-NC、si-SETD5-1~3质粒转染至HT-29细胞中,将其分为对照组(未处理)、si-SETD5-NC组、si-SETD5组和si-SETD5+SC79组,si-SETD5+SC79组HT-29细胞转染质粒的同时用10µmol/L SC79处理。qPCR法检测NCM460、HT-29和LoVo细胞中SETD5 mRNA表达,流式细胞术、细胞划痕法、WB法和CCK-8法分别检测各组HT-29细胞的凋亡情况、迁移能力、相关蛋白的表达,以及对5-FU的敏感性。结果:SETD5 mRNA在HT-29、LoVo细胞中均呈高表达(均P<0.01)。在HT-29细胞中成功地敲减了SETD5 mRNA(P<0.01)。敲减SETD5 mRNA可明显抑制HT-29细胞的增殖活性(P<0.01)、迁移能力(P<0.01)、相关蛋白(SETD5、p-PI3K、p-AKT1、p-mTOR蛋白)的表达(均P<0.01)、促进细胞凋亡(P<0.01),且提高其对5-FU的敏感性(P<0.01),这些作用均可被AKT激活剂SC79部分阻挡(P<0.05或P<0.01)。结论:SETD5在HT-29、LoVo细胞中高表达,SETD5通过PI3K/AKT1通路促进结肠癌HT-29细胞的增殖、迁移,且降低其对5-FU的敏感性,SETD5是结肠癌临床诊断、治疗的潜在靶点。

Geochemistry of sedimentary rocks from Permian–Triassic boundary sections of Tethys Himalaya: implications for paleo-weathering, provenance, and tectonic setting

The geochemical characteristics of two sections—the Permian–Triassic boundary(PTB) Guryul Ravine section, Kashmir Valley, Jammu and Kashmir,India; and the Attargoo section, Spiti Valley, Himachal Pradesh, India—have been studied in the context of provenance, paleo-weathering, and plate tectonic setting.These sections represent the siliciclastic sedimentary sequence from the Tethys Himalaya. The PTB siliciclastic sedimentary sequence in these regions primarily consists of sandstones and shales with variable thickness. Present studied sandstones and shales of both sections had chemical index of alteration values between 65 and 74; such values reveal low-to-moderate degree of chemical weathering. The chemical index of weathering in studied samples ranged from 71 to 94, suggesting a minor K-metasomatism effect on these samples. Plagioclase index of alteration in studied sections ranged from 68 to 92, indicating a moderate degree of weathering of plagioclase feldspars. The provenance discriminant function diagram suggests that the detritus involved in the formation of present studied siliciclastic sedimentary rocks fall in quartzose sedimentary and felsic igneous provenances. These sediments were deposited in a passive continental margin plate tectonic setting according to their location on a Si_2 O versus K_2O/Na_2 O tectonic setting diagram.

miR-127-3p靶向调节SETD8对胃癌细胞增殖、迁移和侵袭的影响

目的探讨微小RNA(miR)-127-3p对胃癌细胞增殖、迁移及侵袭的影响及其作用机制。方法采用qRT-聚合酶链反应(PCR)检测150例胃癌患者胃癌组织、癌旁组织及人胃上皮细胞GES1、人胃癌细胞SGC7901、AGS、MKN-45、MGC-803中miR-127-3p表达水平。双荧光素酶报告基因检测miR-127-3p与含SET结构域赖氨酸甲基转移酶(SETD)8的靶向调控关系。将SGC7901细胞分为对照(Control)组、miR-127-3p模拟物(miR-127-3p mimics)组及miR-NC组、沉默SETD8表达(si-SETD8)组、si-NC组、miR-127-3p mimics+SETD8过表达(miR-127-3p mimics+SETD8)组及空载质粒(miR-127-3p mimics+pcDNA)组。噻唑蓝(MTT)法、划痕愈合实验、Transwell小室实验分别检测SGC7901细胞增殖、迁移与侵袭;Western印迹检测SGC7901细胞SETD8、基质金属蛋白酶(MMP)-2、MMP-9蛋白表达;裸鼠成瘤实验检测过表达miR-127-3p对裸鼠胃癌移植瘤生长的影响。结果miR-127-3p在胃癌组织与细胞中较癌旁组织和正常细胞显著下调(P<0.05),SETD8 mRNA在胃癌组织中较癌旁组织显著上调(P<0.05);胃癌组织中miR-127-3p、SETD8 mRNA表达水平呈负相关(r=-0.553,P<0.05);双荧光素酶实验证实miR-127-3p与SETD8存在靶向调控关系(P<0.05);过表达miR-127-3p或抑制SETD8表达显著抑制SGC7901细胞增殖、迁移与侵袭(P<0.05);过表达SETD8可部分逆转过表达miR-127-3p对SGC7901细胞增殖、迁移与侵袭的抑制作用(P<0.05);裸鼠成瘤实验表明过表达miR-127-3p可显著抑制胃癌移植瘤生长(P<0.05)。结论miR-127-3p在胃癌组织与细胞中表达下调,过表达miR-127-3p可靶向抑制SETD8表达而抑制胃癌肿瘤生长。

Factors Influencing the Outcrossed Seed-Setting Obstacles of Photo-thermo Sensitive Genie Male Sterile Rice

Using photo-thermo sensitive genie male rice (PTGMS) Pei' ai 64S, W7415S, W6154S, N26S, Annong S, Nongken 58S, 7001S and 5088S as female parents and conventional indica lines 8258 and U89 as male parents, the factors affecting outcrossed seed-setting were analyzed. The PTGMS had obstacles in outcrossed seed setting influenced by inheritance and environment at varying degrees. Environmental temperature was regarded as the main factor that resulted in the outcrossed seed-setting obstacles. The sensitive stage was at the early stage of grain filling for outcrossed seed setting. There existed remarkable differences at the sensitivity stage, the duration of sensitive period, the sensitive level and the effective level of outcrossed seed-setting obstacles caused by environmental temperature among different PTGMS lines. Therefore, attention should be paid to outcrossed seed-setting obstacles in selection and utilization of PTGMS lines.

m-degree Center-connecting Line of Finite Points Set and Its Properties

In this paper, we first give the concept of m-degree center-connecting line in n-dimensional Euclidean space Enand investigate its several properties, then we obtain the length of m-degree center-connecting line formula in finite points set. As its application,we extend the Leibniz formula and length of medians formula in n-dimensional simplex to polytope.

Relationship between Beating and Setting-on Place

In this paper,what causes the setting-on place of woven fabrics without adopting tension compensation is explained by means of experimental methods.Owing to that the main shaft speed and the beating speed of reed can not attain the normal values at setting-on,the warp tension and the beating force of the reed can neither reach the normal level.Therefore,the relative slippage movement of the weft against the warp during beating will be less,while the common movement of the weft together with the warp will be bigger than those at nor-mal running.As a result,the setting-on place is

Effect of Stretching - Setting Treatment on Dyeing Behavior of Wool

Changes of dyeing behavior of wool after stretching - setting treatment are studied.Effects of stretching - setting on wool structure are analyzed.It is concluded that stretching - setting treatment not only reduces wool diameter and increases wool length,but also brings about low-temperature dyeing of wool.

On-line Condition Monitoring and Diagnostic Network System for Rotating Machine Set

Several industrial computers and a server are combined to set up the on-line monitoring and diagnostic system of turbo-generator sets. The main function of the system is to monitor machine sets' running condition. Through analyzing running data, technicians can detect whether there exist faults and where they occur. To share and transmit the dynamic information of the turbo-generator sets, a distributed network system is introduced. NetWare network operating system is used in the LAN (Local Area Network) system. The LAN is extended to realize the sharing of data and remote transmission of information. Furthermore, functions of monitoring and diagnostic clients are listed.

LEVEL SET输运方程的求解方法及其对气-液两相流运动界面数值模拟的影响

用数值模拟方法来研究气-液两相流动与传热现象是当今多相流领域的一个热门课题。由于两相流固有的复杂性,气-液两相流界面迁移现象的数值模拟一直是两相流研究中的一大难点。本文介绍了捕捉气-液两相流相界面运动的水平集方法(LevelSet)及其研究进展,介绍了求解LevelSet输运方程的3种方法,即一般差分格式、Superbee-TVD格式和Runge-Kutta法-5阶WENO组合格式。结合主流场的求解,分别用这3种方法对4种典型相界面在5种流场中的迁移特性进行了模拟计算,并对计算结果进行了比较和分析。结果表明,Runge-Kutta法-5阶WENO组合格式求解LevelSet输运方程的效果最好,在以后的计算中将主要采用这种组合格式来进行气-液相界面输运方程的求解。

高磷通过SET8-shRNA调控AKT/Caspase-3通路促进血管平滑肌细胞凋亡

目的探讨高磷通过SET8-shRNA调控AKT/Caspase-3通路促进大鼠血管平滑肌细胞(vascular smooth muscle cells,VSMCs)发生钙化的机制。方法将血管平滑肌细胞随机分为两组:正常组和高磷组(10 mmol/Lβ-甘油磷酸)。采用茜素红染色及钙含量检测血管平滑肌细胞钙化,采用实时荧光定量及Western blot检测SET8、AKT、Caspase-3 mRAN和蛋白表达情况。为验证SET8在细胞钙化中的作用,将大鼠VSMCs分为6组:正常组、空质粒组、SET8低表达组(转染SET8-shRNA质粒)、高磷组(10 mmol/Lβ-甘油磷酸)、空质粒+高磷组(高磷基础上转染SET8过表达的对照质粒)、SET8过表达+高磷组(高磷基础上转染SET8过表达质粒)。检测各组血管平滑肌细胞的钙化、凋亡及AKT、Caspase-3表达情况。为进一步验证AKT在VSMCs钙化中的作用,将血管平滑肌细胞分为3组:AKT抑制剂组(1μl DMSO+AKT抑制剂LY294002)、对照组(1μl DMSO)和正常组,检测AKT、Caspase-3 mRAN和蛋白表达。结果与正常组比较,高磷组细胞钙化结节增多,钙含量增加(P<0.05),STE8和AKT表达降低,Caspase-3表达升高(P<0.05)。与正常组和空质粒组比较,SET8-shRNA组细胞钙化和凋亡增多,AKT表达降低,Caspase-3表达升高(P<0.05);与高磷组和空质粒+高磷组比较,SET8过表达+高磷组细胞钙化和凋亡减少,AKT表达升高,Caspase-3表达降低(P<0.05)。与正常组和对照组比较,AKT抑制剂组AKT表达显著降低,Caspase-3表达显著升高(P<0.05)。结论高磷通过SET8-shRNA抑制AKT活化,进而促进Caspase-3表达,增加VSMCs凋亡,从而影响细胞钙化的发生。

B7-H3、葡萄糖6-磷酸脱氢酶及SET8蛋白在肾透明细胞癌中的表达及与预后的关系分析

目的分析B7-H3、葡萄糖6-磷酸脱氢酶(G6PD)及SET8蛋白在肾透明细胞癌中的表达及与预后的关系。方法收集81例肾透明细胞癌患者的肾透明细胞癌组织及正常组织,比较不同组织中B7-H3、G6PD及SET8蛋白阳性表达情况,随访2年分析肾透明细胞癌预后的影响因素。结果肾透明细胞癌组织中B7-H3、G6PD及SET8蛋白阳性表达率均明显高于正常组织,差异均有统计学意义(P﹤0.01)。多因素分析结果显示,肿瘤直径≥5 cm、TNM分期为Ⅲ-Ⅳ期、有淋巴结转移、细胞分级为G2-3级、B7-H3阳性、G6PD阳性、SET8阳性均为影响肾透明细胞癌患者预后的独立危险因素(P﹤0.05)。结论B7-H3、G6PD及SET8蛋白在肾透明细胞癌中均高表达,在临床中检测三者表达情况对患者预后评估有重要参考价值。

基于SET/MOS混合结构的4-2编码器设计

基于单电子晶体管(SET)的库仑振荡效应、多栅输入特性和相移特性,实现了SET/MOS混合结构的或门逻辑,并利用该或门逻辑实现了4-2编码器。利用HSPICE对所设计电路进行仿真验证,仿真结果表明该电路能够实现4-2编码器的编码功能。与纯CMOS编码器相比,该SET/MOS混合电路仅由2个PMOS管、2个NMOS管和2个SET构成,具有结构简单、尺寸小、集成度高的优点。

谷子(Setaria italica Beauv.)高异交结实雄性不育系“81-16”的柱头性状观察

谷子不育系异交结实率低严重制约着杂交种大面积应用于生产。本研究证明柱头外露程度与异交结实率呈显著正相关(r0.001=0.9597**)。山西省农业科学院经济作物研究所成功选育出谷子光敏雄性不育突变系“81-16”,其不育度为100%,异交结实率达61%。用植物形态解剖学方法证明,“81-16”雌蕊生活力强,柱头完全外露,接受花粉的有效面积大,是其异交结实率高的主要原因。本文还提出应重视母本柱头性状选育,并加强谷子杂交种制种技术研究。

S-rough sets and knowledge separation

The conceptions of the knowledge screen generated by S-rough sets are given: f- screen and - screen , and then puts forward - filter theorem, - filter theorem of knowledge. At last, the applications of knowledge separation are given according to - screen and - screen.

Detecting land subsidence near metro lines in the Baoshan district of Shanghai with multi-temporal interferometric synthetic aperture radar

Land subsidence is a major factor that affects metro line （ML） stability. In this study, an improved multi- temporal interferometric synthetic aperture radar （InSAR） （MTI） method to detect land subsidence near MLs is presented. In particular, our multi-temporal InSAR method provides surface subsidence measurements with high observation density. The MTI method tracks both point-like targets and distributed targets with temporal radar back- scattering steadiness. First, subsidence rates at the point targets with low-amplitude dispersion index （ADI） values are extracted by applying a least-squared estimator on an optimized freely connected network. Second, to reduce error propagation, the pixels with high-ADI values are classified into several groups according to ADI intervals and processed using a Pearson correlation coefficient and hierarchical analysis strategy to obtain the distributed targets. Then, nonlinear subsidence components at all point-like and distributed targets are estimated using phase unwrapping and spatiotemporal filtering on the phase residuals. The proposed MTI method was applied to detect land subsidence near MLs of No. 1 and 3 in the Baoshan district of Shanghai using 18 TerraSAR-X images acquired between April 21, 2008 and October 30, 2010. The results show that the mean subsidence rates of the stations distributed along the two MLs are -12.9 and -14.0 ram/year. Furthermore, three subsidence funnels near the MLs are discovered through the hierarchical analysis. The testing results demonstrate the satisfactory capacity of the proposed MTI method in providing detailed subsidence information near MLs.

MiR-25-3p attenuates the proliferation of tongue squamous cell carcinoma cell line Tca8113

Objective:To investigate the effects of miR-25-3p on the occurrence,development and proliferation of tongue squamous cell carcinoma cells.Methods:To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant reiroviral vector-mediated gene transfer method.The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide(MTT)and cell colony formation assays.eyclnD1,p21^(cipt)and p27^(kipt)mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR.cyclinD1,p21^(cipt),p27^(kipt),AKT,p-AKT,FOXOt and p-FOX01 expressions in the transfected Tca8113 were detected by western blot analysis.In addition,miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR.Results:Quantitative PCR showed that mitt-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue.MTT and cell colony formation assays showed that after miR-25-3p overexpression,the proliferation of transfected Tca8113 was obviously attenuated.Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression.p21^(cipt)and p27^(kipt)expressions were upregulated,while cyclinD1,AKT,FOXO1 expressions were downregulated,and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells.Conclusions:MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression,playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.

γ-（α,β）-Open Sets and γ-（α,β）-Ti Spaces

We introduce the concept of γ-(α, β)-open sets and the concepts of γ-(α, β)-T_i spaces(i = 0, 1, 2, 3, 4) based on the concept of(α, β)-open sets. We obtain some properties of γ-(α, β)-Tispaces. Furthermore, we study their essential characterization in topological space.