On-demand droplet sorting is extensively applied for the efficient manipulation and genome-wide analysis of individual cells.However,state-of-the-art microfluidic chips for droplet sorting still suffer from low sortin...On-demand droplet sorting is extensively applied for the efficient manipulation and genome-wide analysis of individual cells.However,state-of-the-art microfluidic chips for droplet sorting still suffer from low sorting speeds,sample loss,and labor-intensive preparation procedures.Here,we demonstrate the development of a novel microfluidic chip that integrates droplet generation,on-demand electrostatic droplet charging,and high-throughput sorting.The charging electrode is a copper wire buried above the nozzle of the microchannel,and the deflecting electrode is the phosphate buffered saline in the microchannel,which greatly simplifies the structure and fabrication process of the chip.Moreover,this chip is capable of high-frequency droplet generation and sorting,with a frequency of 11.757 kHz in the drop state.The chip completes the selective charging process via electrostatic induction during droplet generation.On-demand charged microdroplets can arbitrarilymove to specific exit channels in a three-dimensional(3D)-deflected electric field,which can be controlled according to user requirements,and the flux of droplet deflection is thereby significantly enhanced.Furthermore,a lossless modification strategy is presented to improve the accuracy of droplet deflection or harvest rate from 97.49% to 99.38% by monitoring the frequency of droplet generation in real time and feeding it back to the charging signal.This chip has great potential for quantitative processing and analysis of single cells for elucidating cell-to-cell variations.展开更多
To improve the efficiency and fairness of the spectrum allocation for ground communication assisted by unmanned aerial vehicles(UAVs),a joint optimization method for on-demand deployment and spectrum allocation of UAV...To improve the efficiency and fairness of the spectrum allocation for ground communication assisted by unmanned aerial vehicles(UAVs),a joint optimization method for on-demand deployment and spectrum allocation of UAVs is proposed,which is modeled as a mixed-integer non-convex optimization problem(MINCOP).An algorithm to estimate the minimum number of required UAVs is firstly proposed based on the pre-estimation and simulated annealing.The MINCOP is then decomposed into three sub-problems based on the block coordinate descent method,including the spectrum allocation of UAVs,the association between UAVs and ground users,and the deployment of UAVs.Specifically,the optimal spectrum allocation is derived based on the interference mitigation and channel reuse.The association between UAVs and ground users is optimized based on local iterated optimization.A particle-based optimization algorithm is proposed to resolve the subproblem of the UAVs deployment.Simulation results show that the proposed method could effectively improve the minimum transmission rate of UAVs as well as user fairness of spectrum allocation.展开更多
Open Courseware(OCW)and massive open online courses(MOOCs)are teaching and learning resources that are easily accessible to anyone with an Internet connection.OCW is digitally published learning content including full...Open Courseware(OCW)and massive open online courses(MOOCs)are teaching and learning resources that are easily accessible to anyone with an Internet connection.OCW is digitally published learning content including full and partial courses(syllabi,outlines,lectures in pdf or video,slides,reference lists,etc.),simulations,animations,tutorials,drills and practices,modules,podcasts,case studies,and quizzes.This content is free and can be adopted or adapted to the user's needs.MOOCs are online learning experiences taught by university professors using conventional educational tools including video lectures,interactive modules,assignments,study materials,discussion boards,quizzes and tests.MOOCs are offered free or at low cost for personal and professional learning,and as a supplement to classroom teaching.Many MOOCs and OCW focus on topics of interest to nursing,particularly to nurse educators.This article provides the reader with a brief history of the development of OCW and MOOCs,conceptual descriptions,and guidance about how to access and use these new online resources.展开更多
The development of technology will affect the development of traditional dance classes.The teaching method is no longer a singular course of teaching and learning.Multimedia courseware is a commonly used course techno...The development of technology will affect the development of traditional dance classes.The teaching method is no longer a singular course of teaching and learning.Multimedia courseware is a commonly used course technology,based on computer and projection technology,to convey rich knowledge to students.This paper discusses the use of multimedia courseware in dance classes,then,combined with the majors the author have studied,creatively discusses the influence of multimedia courseware on Chinese folk dance courses.展开更多
Aimed at the needs of developing the CAI courseware with popularization, the expression ways for hypercard based information, and the key principles of the development for authoring tools, were discussed. The ideas of...Aimed at the needs of developing the CAI courseware with popularization, the expression ways for hypercard based information, and the key principles of the development for authoring tools, were discussed. The ideas of the revised hypermedia chaining structure were introduced. The framework of the courseware authoring tool, its function design and the methods of technical realization for system software were also involved.展开更多
In this paper, we conduct research on the developmental trend of the modern multimedia technology and the influences on the courseware making. Compared with the traditional classroom teaching courseware, distance educ...In this paper, we conduct research on the developmental trend of the modern multimedia technology and the influences on the courseware making. Compared with the traditional classroom teaching courseware, distance education of multimedia courseware and have a lot in common, also have different places. Therefore, we should be based on the characteristics of the distance education, carefully study the requirements of teaching courseware, to grasp the principle of remote multimedia teaching courseware design, production, strive to produce high quality courseware. Make full use of multimedia technology provides a variety of input and output functions, combined with the network transmission, make multimedia CAI courseware has the very strong interactivity as the introduction of hypertext structure, as the design method of interactive multimedia courseware to bring very great convenience. Our research proposes the novel paradigm for the issues that is meaningful.展开更多
Aerobics teaching in colleges and universities is the most important part of higher education, and the important means to carry out quality-oriented education and cultivate all-round developed talents. The reform of a...Aerobics teaching in colleges and universities is the most important part of higher education, and the important means to carry out quality-oriented education and cultivate all-round developed talents. The reform of aerobics teaching in colleges and universities should be geared to the demands of the times, and explore new innovation and continual development. In this paper, using the research methods of documentation, courseware development, teaching experiment and mathematical statistics, we study on the making and application of college aerobics network teaching courseware. It is revealed that, with the combination of modem network technology and college aerobics teaching, the resulted college aerobics network teaching courseware is the important media to not only upgrades college aerobics teaching quality, but also help develop students＇ habits of doing physical exercise, and promote physical health. Therefore, this is meaningful and practical in promoting the education of college students in physical quality development.展开更多
With the acceleration of the pace of social life and the emergence of the convenience economy,the requirements for on-demand cooking services have increased due to the increasing demand for food quality and the health...With the acceleration of the pace of social life and the emergence of the convenience economy,the requirements for on-demand cooking services have increased due to the increasing demand for food quality and the health concerns associated with takeaway food,as well as the lack of cooking skills.This paper aims to analyze the business model of on-demand cooking service platforms in China with the theory of Business Model Canvas proposed by Alexander Osterwalder.By exploring the advantages and disadvantages of the business model of on-demand door-to-door cooking,the study seeks to provide relevant recommendations.展开更多
To meet the bandwidth requirement for the multicasting data flow in ad hoc networks, a distributed on- demand bandwidth-constrained multicast routing （BCMR） protocol for wireless ad hoc networks is proposed. With th...To meet the bandwidth requirement for the multicasting data flow in ad hoc networks, a distributed on- demand bandwidth-constrained multicast routing （BCMR） protocol for wireless ad hoc networks is proposed. With this protocol, the resource reservation table of each node will record the bandwidth requirements of data flows, which access itself, its neighbor nodes and hidden nodes, and every node calculates the remaining available bandwidth by deducting the bandwidth reserved in the resource reservation table from the total available bandwidth of the node. Moreover, the BCMR searches in a distributed manner for the paths with the shortest delay conditioned by the bandwidth constraint. Simulation results demonstrate the good performance of BCMR in terms of packet delivery reliability and the delay. BCMR can meet the requirements of real time communication and can be used in the multicast applications with low mobility in wireless ad hoc networks.展开更多
Internet of Things(IoT)networks are characterized by a multitude of wireless,interconnected devices that can dynamically join or exit the network without centralized administration or fixed infrastructure for routing....Internet of Things(IoT)networks are characterized by a multitude of wireless,interconnected devices that can dynamically join or exit the network without centralized administration or fixed infrastructure for routing.While multipath routing in IoT networks can improve data transmission reliability and load balancing by establishing multiple paths between source and destination nodes,these networks are susceptible to security threats due to their wireless nature.Traditional security solutions developed for conventional networks are often ill-suited to the unique challenges posed by IoT environments.In response to these challenges,this paper proposes the integration of the Ad hoc On-demand Multipath Distance Vector(AOMDV)routing protocol with a trust model to enhance network performance.Key findings from this research demonstrate the successful fusion of AOMDV with a trust model,resulting in tangible improvements in network performance.The assessment of trustworthiness bolsters both security and routing capabilities in IoT networks.The trust model plays a crucial role in mitigating black hole attacks in IoT networks by evaluating the trustworthiness of nodes and helping in the identification and avoidance of malicious nodes that may act as black holes.Simulation results validate the efficacy of the proposed trust-based routing mechanism in achieving its objectives.Trust plays a pivotal role in decision-making and in the creation of secure distribution systems.By assessing the trustworthiness of nodes,both network security and routing efficiency can be enhanced.The effectiveness of the proposed trust-based routing mechanism is scrutinized through simulations,offering insights into its potential advantages in terms of improved network security and routing performance in the context of the IoT.展开更多
Various transportation systems have been developed in recent years.In this study,an artificial society model is developed to examine the combination of transportation policies in urban areas.In this model,each trip ma...Various transportation systems have been developed in recent years.In this study,an artificial society model is developed to examine the combination of transportation policies in urban areas.In this model,each trip maker selects the primary and terminal transportation modes.An artificial society model is applied to the southeastern region of Osaka City,Japan.The effects of introducing BRT(bus rapid transit,primary transportation)and on-demand buses(terminal transportation)are investigated.The results confirm that BRT is used by a certain number of users.An increase in the use of BRT will increase the amount of walking,thus resulting in a healthy city.However,on-demand buses are rarely used as terminal transportation.Additionally,the development of bicycle parking stations near BRT stops is shown to be effective in the northern section of the BRT route.展开更多
基金The authors acknowledge the financial support from the NationalNatural Science Foundation ofChina(No.52275562)the Technology Innovation Fund of Huazhong University of Science and Technology(No.2022JYCXJJ015).
文摘On-demand droplet sorting is extensively applied for the efficient manipulation and genome-wide analysis of individual cells.However,state-of-the-art microfluidic chips for droplet sorting still suffer from low sorting speeds,sample loss,and labor-intensive preparation procedures.Here,we demonstrate the development of a novel microfluidic chip that integrates droplet generation,on-demand electrostatic droplet charging,and high-throughput sorting.The charging electrode is a copper wire buried above the nozzle of the microchannel,and the deflecting electrode is the phosphate buffered saline in the microchannel,which greatly simplifies the structure and fabrication process of the chip.Moreover,this chip is capable of high-frequency droplet generation and sorting,with a frequency of 11.757 kHz in the drop state.The chip completes the selective charging process via electrostatic induction during droplet generation.On-demand charged microdroplets can arbitrarilymove to specific exit channels in a three-dimensional(3D)-deflected electric field,which can be controlled according to user requirements,and the flux of droplet deflection is thereby significantly enhanced.Furthermore,a lossless modification strategy is presented to improve the accuracy of droplet deflection or harvest rate from 97.49% to 99.38% by monitoring the frequency of droplet generation in real time and feeding it back to the charging signal.This chip has great potential for quantitative processing and analysis of single cells for elucidating cell-to-cell variations.
基金supported by Project funded by China Postdoctoral Science Foundation(No.2021MD703980)。
文摘To improve the efficiency and fairness of the spectrum allocation for ground communication assisted by unmanned aerial vehicles(UAVs),a joint optimization method for on-demand deployment and spectrum allocation of UAVs is proposed,which is modeled as a mixed-integer non-convex optimization problem(MINCOP).An algorithm to estimate the minimum number of required UAVs is firstly proposed based on the pre-estimation and simulated annealing.The MINCOP is then decomposed into three sub-problems based on the block coordinate descent method,including the spectrum allocation of UAVs,the association between UAVs and ground users,and the deployment of UAVs.Specifically,the optimal spectrum allocation is derived based on the interference mitigation and channel reuse.The association between UAVs and ground users is optimized based on local iterated optimization.A particle-based optimization algorithm is proposed to resolve the subproblem of the UAVs deployment.Simulation results show that the proposed method could effectively improve the minimum transmission rate of UAVs as well as user fairness of spectrum allocation.
文摘Open Courseware(OCW)and massive open online courses(MOOCs)are teaching and learning resources that are easily accessible to anyone with an Internet connection.OCW is digitally published learning content including full and partial courses(syllabi,outlines,lectures in pdf or video,slides,reference lists,etc.),simulations,animations,tutorials,drills and practices,modules,podcasts,case studies,and quizzes.This content is free and can be adopted or adapted to the user's needs.MOOCs are online learning experiences taught by university professors using conventional educational tools including video lectures,interactive modules,assignments,study materials,discussion boards,quizzes and tests.MOOCs are offered free or at low cost for personal and professional learning,and as a supplement to classroom teaching.Many MOOCs and OCW focus on topics of interest to nursing,particularly to nurse educators.This article provides the reader with a brief history of the development of OCW and MOOCs,conceptual descriptions,and guidance about how to access and use these new online resources.
文摘The development of technology will affect the development of traditional dance classes.The teaching method is no longer a singular course of teaching and learning.Multimedia courseware is a commonly used course technology,based on computer and projection technology,to convey rich knowledge to students.This paper discusses the use of multimedia courseware in dance classes,then,combined with the majors the author have studied,creatively discusses the influence of multimedia courseware on Chinese folk dance courses.
文摘Aimed at the needs of developing the CAI courseware with popularization, the expression ways for hypercard based information, and the key principles of the development for authoring tools, were discussed. The ideas of the revised hypermedia chaining structure were introduced. The framework of the courseware authoring tool, its function design and the methods of technical realization for system software were also involved.
文摘In this paper, we conduct research on the developmental trend of the modern multimedia technology and the influences on the courseware making. Compared with the traditional classroom teaching courseware, distance education of multimedia courseware and have a lot in common, also have different places. Therefore, we should be based on the characteristics of the distance education, carefully study the requirements of teaching courseware, to grasp the principle of remote multimedia teaching courseware design, production, strive to produce high quality courseware. Make full use of multimedia technology provides a variety of input and output functions, combined with the network transmission, make multimedia CAI courseware has the very strong interactivity as the introduction of hypertext structure, as the design method of interactive multimedia courseware to bring very great convenience. Our research proposes the novel paradigm for the issues that is meaningful.
文摘Aerobics teaching in colleges and universities is the most important part of higher education, and the important means to carry out quality-oriented education and cultivate all-round developed talents. The reform of aerobics teaching in colleges and universities should be geared to the demands of the times, and explore new innovation and continual development. In this paper, using the research methods of documentation, courseware development, teaching experiment and mathematical statistics, we study on the making and application of college aerobics network teaching courseware. It is revealed that, with the combination of modem network technology and college aerobics teaching, the resulted college aerobics network teaching courseware is the important media to not only upgrades college aerobics teaching quality, but also help develop students＇ habits of doing physical exercise, and promote physical health. Therefore, this is meaningful and practical in promoting the education of college students in physical quality development.
文摘With the acceleration of the pace of social life and the emergence of the convenience economy,the requirements for on-demand cooking services have increased due to the increasing demand for food quality and the health concerns associated with takeaway food,as well as the lack of cooking skills.This paper aims to analyze the business model of on-demand cooking service platforms in China with the theory of Business Model Canvas proposed by Alexander Osterwalder.By exploring the advantages and disadvantages of the business model of on-demand door-to-door cooking,the study seeks to provide relevant recommendations.
基金The Natural Science Foundation of Zhejiang Province(No.Y1090232)
文摘To meet the bandwidth requirement for the multicasting data flow in ad hoc networks, a distributed on- demand bandwidth-constrained multicast routing （BCMR） protocol for wireless ad hoc networks is proposed. With this protocol, the resource reservation table of each node will record the bandwidth requirements of data flows, which access itself, its neighbor nodes and hidden nodes, and every node calculates the remaining available bandwidth by deducting the bandwidth reserved in the resource reservation table from the total available bandwidth of the node. Moreover, the BCMR searches in a distributed manner for the paths with the shortest delay conditioned by the bandwidth constraint. Simulation results demonstrate the good performance of BCMR in terms of packet delivery reliability and the delay. BCMR can meet the requirements of real time communication and can be used in the multicast applications with low mobility in wireless ad hoc networks.
文摘Internet of Things(IoT)networks are characterized by a multitude of wireless,interconnected devices that can dynamically join or exit the network without centralized administration or fixed infrastructure for routing.While multipath routing in IoT networks can improve data transmission reliability and load balancing by establishing multiple paths between source and destination nodes,these networks are susceptible to security threats due to their wireless nature.Traditional security solutions developed for conventional networks are often ill-suited to the unique challenges posed by IoT environments.In response to these challenges,this paper proposes the integration of the Ad hoc On-demand Multipath Distance Vector(AOMDV)routing protocol with a trust model to enhance network performance.Key findings from this research demonstrate the successful fusion of AOMDV with a trust model,resulting in tangible improvements in network performance.The assessment of trustworthiness bolsters both security and routing capabilities in IoT networks.The trust model plays a crucial role in mitigating black hole attacks in IoT networks by evaluating the trustworthiness of nodes and helping in the identification and avoidance of malicious nodes that may act as black holes.Simulation results validate the efficacy of the proposed trust-based routing mechanism in achieving its objectives.Trust plays a pivotal role in decision-making and in the creation of secure distribution systems.By assessing the trustworthiness of nodes,both network security and routing efficiency can be enhanced.The effectiveness of the proposed trust-based routing mechanism is scrutinized through simulations,offering insights into its potential advantages in terms of improved network security and routing performance in the context of the IoT.
基金supported by JSPS KAKENHI(grant number:21K04307).
文摘Various transportation systems have been developed in recent years.In this study,an artificial society model is developed to examine the combination of transportation policies in urban areas.In this model,each trip maker selects the primary and terminal transportation modes.An artificial society model is applied to the southeastern region of Osaka City,Japan.The effects of introducing BRT(bus rapid transit,primary transportation)and on-demand buses(terminal transportation)are investigated.The results confirm that BRT is used by a certain number of users.An increase in the use of BRT will increase the amount of walking,thus resulting in a healthy city.However,on-demand buses are rarely used as terminal transportation.Additionally,the development of bicycle parking stations near BRT stops is shown to be effective in the northern section of the BRT route.
