A new approach is presented for preparative,continuous flow fractionation of sub-10-kbp DNA fragments,which exploits the variation in the field-dependent mobility of the DNA molecules based on their length.Orthogonall...A new approach is presented for preparative,continuous flow fractionation of sub-10-kbp DNA fragments,which exploits the variation in the field-dependent mobility of the DNA molecules based on their length.Orthogonally pulsed electric fields of significantly different magnitudes are applied to a microchip filled with a sieving matrix of 1.2% agarose gel.Using this method,we demonstrate a high-resolution separation of 0.5,1,2,5,and 10 kbp DNA fragments within 2 min.During the separation,DNA fragments are also purified from other ionic species.Preparative fractionation of sub-10-kbp DNA molecules plays an important role in second-generation sequencing.The presented device performs rapid high-resolution fractionation and it can be reliably manufactured with simple microfabrication procedures.展开更多
文摘A new approach is presented for preparative,continuous flow fractionation of sub-10-kbp DNA fragments,which exploits the variation in the field-dependent mobility of the DNA molecules based on their length.Orthogonally pulsed electric fields of significantly different magnitudes are applied to a microchip filled with a sieving matrix of 1.2% agarose gel.Using this method,we demonstrate a high-resolution separation of 0.5,1,2,5,and 10 kbp DNA fragments within 2 min.During the separation,DNA fragments are also purified from other ionic species.Preparative fractionation of sub-10-kbp DNA molecules plays an important role in second-generation sequencing.The presented device performs rapid high-resolution fractionation and it can be reliably manufactured with simple microfabrication procedures.
