Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSP...Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.展开更多
In this paper,we considered the Length-biased weighted Lomax distribution and constructed new acceptance sampling plans(ASPs)where the life test is assumed to be truncated at a pre-assigned time.For the new suggested ...In this paper,we considered the Length-biased weighted Lomax distribution and constructed new acceptance sampling plans(ASPs)where the life test is assumed to be truncated at a pre-assigned time.For the new suggested ASPs,the tables of the minimum samples sizes needed to assert a specific mean life of the test units are obtained.In addition,the values of the corresponding operating characteristic function and the associated producer’s risks are calculated.Analyses of two real data sets are presented to investigate the applicability of the proposed acceptance sampling plans;one data set contains the first failure of 20 small electric carts,and the other data set contains the failure times of the air conditioning system of an airplane.Comparisons are made between the proposed acceptance sampling plans and some existing acceptance sampling plans considered in this study based on the minimum sample sizes.It is observed that the samples sizes based on the proposed acceptance sampling plans are less than their competitors considered in this study.The suggested acceptance sampling plans are recommended for practitioners in the field.展开更多
According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's k...According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.展开更多
Regardless of which (model-centric or code-centric) development process is adopted, industrial software production ultimately and necessarily requires the delivery of an executable implementation. It is generally acce...Regardless of which (model-centric or code-centric) development process is adopted, industrial software production ultimately and necessarily requires the delivery of an executable implementation. It is generally accepted that the quality of such an implementation is of utmost importance. Yet current verification techniques, including software testing, remain problematic. In this paper, we focus on acceptance testing, that is, on the validation of the actual behavior of the implementation under test against the requirements of stakeholder(s). This task must be as objective and automated as possible. Our first goal is to review existing code-based and model-based tools for testing in light of what such an objective and automated approach to acceptance testing entails. Our contention is that the difficulties we identify originate mainly in a lack of traceability between a testable model of the requirements of the stakeholder(s) and the test cases used to validate these requirements. We then investigate whether such traceability is addressed in other relevant specification-based approaches.展开更多
Based on the experience of environmental acceptance tests for power transmission projects, the problems found in acceptance tests and the system shortcomings of acceptance test itself are analyzed. Recommendations are...Based on the experience of environmental acceptance tests for power transmission projects, the problems found in acceptance tests and the system shortcomings of acceptance test itself are analyzed. Recommendations are provided to improve the future work.展开更多
A cable circuit of a substation in the United Kingdom showed high level of PD activities during a survey using hand hold PD testing equipment. The authors were invited to carry out on-site PD testing experiment to fur...A cable circuit of a substation in the United Kingdom showed high level of PD activities during a survey using hand hold PD testing equipment. The authors were invited to carry out on-site PD testing experiment to further diagnose and locate the potential problem of the cable system. This paper presents the experience of the present authors carrying out the cable test. Following a brief introduction to the experiment equipments and physical connections, the paper analyses the data collected from the testing, including PD pulse shape analysis, frequency spectrum analysis and phase resolved PD pattern analysis. Associated with PD propagation direction identification, PD source diagnosis and localisation was made. Four different types of sensors, which were adapted during the testing, are shown to have different frequency bandwidths and performed differently. Aider comparing the parameters of the sensor and the PD signals detected by individual sensor, optimal PD monitoring bandwidth for cable system is suggested.展开更多
On May 28, the 120-ton thrust liquid-oxygen/kerosene engine developed by the Academy of Aerospace Liquid Propulsion Technology (AALPT) passed the acceptance test organized by China National Space Administration (CNSA)...On May 28, the 120-ton thrust liquid-oxygen/kerosene engine developed by the Academy of Aerospace Liquid Propulsion Technology (AALPT) passed the acceptance test organized by China National Space Administration (CNSA). The 120-ton thrust liquid-oxygen/kerosene engine is a non-pollution, non-toxic, high performance and reliable basic pro-展开更多
In recent years,the telecommunications sector is no longer limited to traditional communications,but has become the backbone for the use of data,content and digital applications by individuals,governments and companie...In recent years,the telecommunications sector is no longer limited to traditional communications,but has become the backbone for the use of data,content and digital applications by individuals,governments and companies to ensure the continuation of economic and social activity in light of social distancing and total closure inmost countries in the world.Therefore,electronic government(e-Government)andmobile government(m-Government)are the results of technological evolution and innovation.Hence,it is important to investigate the factors that influence the intention to use m-Government services among Jordan’s society.This paper proposed a new m-Government acceptance model in Jordan(AMGS);this model combines the Information System(IS)Success Factor Model and Hofstede Cultural Dimensions Theory.The study was conducted by surveying different groups of the Jordanian community.Astructured questionnaire was used to collect data from203 respondents.Multiple regression analysis has been conducted to analyze the data.The results indicate that the significant predictors of citizen intention to use m-Government services in Jordan are Information Quality,Service Quality,Uncertainty Avoidance,and Indulgence vs.restraint.While,the results also suggest that Power Distance is not a significant predictor of citizen intention to use m-Government services.展开更多
Globally, there is increasing evidence that HIV self-testing (HIVST) is commonly practiced among health care workers (HCWs). Although HIVST kits are available in South Africa, their distribution and use remain unregul...Globally, there is increasing evidence that HIV self-testing (HIVST) is commonly practiced among health care workers (HCWs). Although HIVST kits are available in South Africa, their distribution and use remain unregulated and its acceptability, undocumented. The aim of the study was to explore the acceptability of HIV self-testing among student nurses, examine their willingness to use self-testing, and evaluate their views on the implementation of a regulated HIV self-testing programme. This was a qualitative explorative study, and six focus group discussions were conducted with 67 student nurses recruited from a private nursing college, in Pretoria, South Africa. Thematic analysis and NVivo10 qualitative software were used for data analysis. The nurses perceived HIVST as an acceptable option for HIV testing and most were willing to self-test. Self-testing will provide confidential and accessible HIV testing services, reduce the stigma associated with the condition, and increase partner testing and disclosure. HIVST is more likely to be practised if self-testing kits are easily accessible and affordable, and there are strategies to educate people before the adoption of HIVST. The lack of pre- and post-test counselling, the risk of inaccurate test results, and lack of strategies for the confirmation of self-testing results were the main arguments against HIVST. The high acceptance of HIVST among nurses suggests that HIVST is a feasible option to increase the uptake of HIV-testing among HCWs. However, pre- and post-test counselling should be considered important components in the implementation of self-testing services. Educational and awareness campaigns should precede the roll-out of the self-testing programs.展开更多
With the continuous increase of mining in depth,the gas extraction faces the challenges of low permeability,great ground stress,high temperature and large gas pressure in coal seam.The controllable shock wave(CSW),as ...With the continuous increase of mining in depth,the gas extraction faces the challenges of low permeability,great ground stress,high temperature and large gas pressure in coal seam.The controllable shock wave(CSW),as a new method for enhancing permeability of coal seam to improve gas extraction,features in the advantages of high efficiency,eco-friendly,and low cost.In order to better utilize the CSW into gas extraction in coal mine,the mechanism and feasibility of CSW enhanced extraction need to be studied.In this paper,the basic principles,the experimental tests,the mathematical models,and the on-site tests of CSW fracturing coal seams are reviewed,thereby its future research directions are provided.Based on the different media between electrodes,the CSW can be divided into three categories:hydraulic effect,wire explosion and excitation of energetic materials by detonating wire.During the process of propagation and attenuation of the high-energy shock wave in coal,the shock wave and bubble pulsation work together to produce an enhanced permeability effect on the coal seam.The stronger the strength of the CSW is,the more cracks created in the coal is,and the greater the length,width and area of the cracks being.The repeated shock on the coal seam is conducive to the formation of complex network fracture system as well as the reduction of coal seam strength,but excessive shock frequency will also damage the coal structure,resulting in the limited effect of the enhanced gas extraction.Under the influence of ground stress,the crack propagation in coal seam will be restrained.The difference of horizontal principal stress has a significant impact on the shape,propagation direction and connectivity of the CSW induced cracks.The permeability enhancement effect of CSW is affected by the breakage degree of coal seam.The shock wave is absorbed by the broken coal,which may hinder the propagation of CSW,resulting in a poor effect of permeability enhancement.When arranging two adjacent boreholes for CSW permeability enhancement test,the spacing of boreholes should not be too close,which may lead to negative pressure mutual pulling in the early stage of drainage.At present,the accurate method for effectively predicting the CSW permeability enhanced range should be further investigated.展开更多
基金supported by the Scientific and Innovative Action Plan of Shanghai(21N31900800)Shanghai Rising-Star Program(23QB1403500)+4 种基金the Shanghai Sailing Program(20YF1443000)Shanghai Science and Technology Commission,the Belt and Road Project(20310750500)Talent Project of SAAS(2023-2025)Runup Plan of SAAS(ZP22211)the SAAS Program for Excellent Research Team(2022(B-16))。
文摘Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.
基金funding this work through the Research Groups Program under Grant Number R.G.P.2/68/41.I.A.
文摘In this paper,we considered the Length-biased weighted Lomax distribution and constructed new acceptance sampling plans(ASPs)where the life test is assumed to be truncated at a pre-assigned time.For the new suggested ASPs,the tables of the minimum samples sizes needed to assert a specific mean life of the test units are obtained.In addition,the values of the corresponding operating characteristic function and the associated producer’s risks are calculated.Analyses of two real data sets are presented to investigate the applicability of the proposed acceptance sampling plans;one data set contains the first failure of 20 small electric carts,and the other data set contains the failure times of the air conditioning system of an airplane.Comparisons are made between the proposed acceptance sampling plans and some existing acceptance sampling plans considered in this study based on the minimum sample sizes.It is observed that the samples sizes based on the proposed acceptance sampling plans are less than their competitors considered in this study.The suggested acceptance sampling plans are recommended for practitioners in the field.
基金sponsored by the Department of Earthquake Monitoring and Prediction,China Earthquake Administration
文摘According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.
文摘Regardless of which (model-centric or code-centric) development process is adopted, industrial software production ultimately and necessarily requires the delivery of an executable implementation. It is generally accepted that the quality of such an implementation is of utmost importance. Yet current verification techniques, including software testing, remain problematic. In this paper, we focus on acceptance testing, that is, on the validation of the actual behavior of the implementation under test against the requirements of stakeholder(s). This task must be as objective and automated as possible. Our first goal is to review existing code-based and model-based tools for testing in light of what such an objective and automated approach to acceptance testing entails. Our contention is that the difficulties we identify originate mainly in a lack of traceability between a testable model of the requirements of the stakeholder(s) and the test cases used to validate these requirements. We then investigate whether such traceability is addressed in other relevant specification-based approaches.
文摘Based on the experience of environmental acceptance tests for power transmission projects, the problems found in acceptance tests and the system shortcomings of acceptance test itself are analyzed. Recommendations are provided to improve the future work.
文摘A cable circuit of a substation in the United Kingdom showed high level of PD activities during a survey using hand hold PD testing equipment. The authors were invited to carry out on-site PD testing experiment to further diagnose and locate the potential problem of the cable system. This paper presents the experience of the present authors carrying out the cable test. Following a brief introduction to the experiment equipments and physical connections, the paper analyses the data collected from the testing, including PD pulse shape analysis, frequency spectrum analysis and phase resolved PD pattern analysis. Associated with PD propagation direction identification, PD source diagnosis and localisation was made. Four different types of sensors, which were adapted during the testing, are shown to have different frequency bandwidths and performed differently. Aider comparing the parameters of the sensor and the PD signals detected by individual sensor, optimal PD monitoring bandwidth for cable system is suggested.
文摘On May 28, the 120-ton thrust liquid-oxygen/kerosene engine developed by the Academy of Aerospace Liquid Propulsion Technology (AALPT) passed the acceptance test organized by China National Space Administration (CNSA). The 120-ton thrust liquid-oxygen/kerosene engine is a non-pollution, non-toxic, high performance and reliable basic pro-
基金This research funded by Al-Zaytoonah University of Jordan.
文摘In recent years,the telecommunications sector is no longer limited to traditional communications,but has become the backbone for the use of data,content and digital applications by individuals,governments and companies to ensure the continuation of economic and social activity in light of social distancing and total closure inmost countries in the world.Therefore,electronic government(e-Government)andmobile government(m-Government)are the results of technological evolution and innovation.Hence,it is important to investigate the factors that influence the intention to use m-Government services among Jordan’s society.This paper proposed a new m-Government acceptance model in Jordan(AMGS);this model combines the Information System(IS)Success Factor Model and Hofstede Cultural Dimensions Theory.The study was conducted by surveying different groups of the Jordanian community.Astructured questionnaire was used to collect data from203 respondents.Multiple regression analysis has been conducted to analyze the data.The results indicate that the significant predictors of citizen intention to use m-Government services in Jordan are Information Quality,Service Quality,Uncertainty Avoidance,and Indulgence vs.restraint.While,the results also suggest that Power Distance is not a significant predictor of citizen intention to use m-Government services.
文摘Globally, there is increasing evidence that HIV self-testing (HIVST) is commonly practiced among health care workers (HCWs). Although HIVST kits are available in South Africa, their distribution and use remain unregulated and its acceptability, undocumented. The aim of the study was to explore the acceptability of HIV self-testing among student nurses, examine their willingness to use self-testing, and evaluate their views on the implementation of a regulated HIV self-testing programme. This was a qualitative explorative study, and six focus group discussions were conducted with 67 student nurses recruited from a private nursing college, in Pretoria, South Africa. Thematic analysis and NVivo10 qualitative software were used for data analysis. The nurses perceived HIVST as an acceptable option for HIV testing and most were willing to self-test. Self-testing will provide confidential and accessible HIV testing services, reduce the stigma associated with the condition, and increase partner testing and disclosure. HIVST is more likely to be practised if self-testing kits are easily accessible and affordable, and there are strategies to educate people before the adoption of HIVST. The lack of pre- and post-test counselling, the risk of inaccurate test results, and lack of strategies for the confirmation of self-testing results were the main arguments against HIVST. The high acceptance of HIVST among nurses suggests that HIVST is a feasible option to increase the uptake of HIV-testing among HCWs. However, pre- and post-test counselling should be considered important components in the implementation of self-testing services. Educational and awareness campaigns should precede the roll-out of the self-testing programs.
文摘目的对普通话噪声接受度追踪(tracking of noise tolerance,TNT)测试材料进行复测信度评估,并比较测试结果的平均可容忍噪声水平(average tolerable noise level,aTNL)与程序估算的TNL值(estimated tolerable noise level,eTNL)是否存在差异。方法对25例听力言语正常的成年受试者在测试当天先后进行两次普通话版TNT测试[分别为测试一(C1),测试二(C2)],13例于1周后进行复测[测试三(C3),言语材料主题同C2],三次测试的刺激声条件均为言语声0°,连续语谱噪声180°。通过比较C1与C2、C2与C3的TNT分数和aTNL值的相关性来分别评估普通话版TNT测试材料期内、期间测试的复测信度。结果25例受试者C1、C2的aTNL值分别为83.42±3.09、83.50±3.18 dB SPL,eTNL值分别为84.08±3.53、83.95±3.85 dB SPL;13例受试者C3的aTNL值和eTNL值分别为83.16±2.13、83.18±2.64 dB SPL。期内比较(C1与C2)、期间比较(C2与C3)的TNT分数差值的95%CI分别为2.36、2.75 dB,Pearson相关性分析显示期内(r=0.837,P<0.001)、期间(r=0.867,P<0.001)的复测信度良好。除C1的aTNL与eTNL存在统计学差异(P<0.05)之外,其余TNT测试的aTNL与eTNL均无统计学差异(均P>0.05),且90%以上的受试者可在1分钟内获得eTNL。结论普通话版TNT测试的复测信度良好,具有高可靠性、高效性。
基金National Natural Science Foundation of China(52004117,52174117 and 52074146)Postdoctoral Science Foundation of China(2021T140290 and 2020M680975)Basic scientific research project of Liaoning Provincial Department of Education(JYTZD2023073).
文摘With the continuous increase of mining in depth,the gas extraction faces the challenges of low permeability,great ground stress,high temperature and large gas pressure in coal seam.The controllable shock wave(CSW),as a new method for enhancing permeability of coal seam to improve gas extraction,features in the advantages of high efficiency,eco-friendly,and low cost.In order to better utilize the CSW into gas extraction in coal mine,the mechanism and feasibility of CSW enhanced extraction need to be studied.In this paper,the basic principles,the experimental tests,the mathematical models,and the on-site tests of CSW fracturing coal seams are reviewed,thereby its future research directions are provided.Based on the different media between electrodes,the CSW can be divided into three categories:hydraulic effect,wire explosion and excitation of energetic materials by detonating wire.During the process of propagation and attenuation of the high-energy shock wave in coal,the shock wave and bubble pulsation work together to produce an enhanced permeability effect on the coal seam.The stronger the strength of the CSW is,the more cracks created in the coal is,and the greater the length,width and area of the cracks being.The repeated shock on the coal seam is conducive to the formation of complex network fracture system as well as the reduction of coal seam strength,but excessive shock frequency will also damage the coal structure,resulting in the limited effect of the enhanced gas extraction.Under the influence of ground stress,the crack propagation in coal seam will be restrained.The difference of horizontal principal stress has a significant impact on the shape,propagation direction and connectivity of the CSW induced cracks.The permeability enhancement effect of CSW is affected by the breakage degree of coal seam.The shock wave is absorbed by the broken coal,which may hinder the propagation of CSW,resulting in a poor effect of permeability enhancement.When arranging two adjacent boreholes for CSW permeability enhancement test,the spacing of boreholes should not be too close,which may lead to negative pressure mutual pulling in the early stage of drainage.At present,the accurate method for effectively predicting the CSW permeability enhanced range should be further investigated.
