A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Role of macroscopic on-site evaluation of endoscopic ultrasoundguided fine-needle aspiration/biopsy:Results of a multicentric prospective study

BACKGROUND The concept of macroscopic on-site evaluation(MOSE)was introduced in 2015 when the endoscopist observed better diagnostic yield when the macroscopically visible core on MOSE was superior to 4 mm.Recent studies suggest that MOSE by the endoscopist may be an excellent alternative to rapid on-site evaluation,and some classi-fications have been published.Few studies have assessed the adequacy of histologic cores in MOSE during endoscopic ultrasound-guided fine-needle aspiration/biopsy(EUS-FNA/FNB).AIM To evaluate the performance of MOSE during EUS-FNA/FNB.METHODS This multicentric prospective study was conducted in 16 centers in 3 countries(Egypt,Iraq,and Morocco)and included 1108 patients with pancreatic,biliary,or gastrointestinal pathology who were referred for EUS examination.We prospectively analyzed the MOSE in 1008 patients with available histopathological reports according to 2 classifications to determine the adequacy of the histological core samples.Data management and analysis were performed using a Statistical Package for Social Sciences(SPSS)version 27.RESULTS A total of 1074 solid lesions were biopsied in 1008 patients with available cytopathological reports.Mean age was 59 years,and 509 patients(50.5%)were male.The mean lesion size was 38 mm.The most frequently utilized needles were FNB-Franseen(74.5%)and 22 G(93.4%),with a median of 2 passes.According to 2 classifications,618 non-bloody cores(61.3%)and 964 good samples(95.6%)were adequate for histological evaluation.The overall diagnostic yield of cytopathology was 95.5%.The cytological examination confirmed the diagnosis of malignancy in 861 patients(85.4%),while 45 samples(4.5%)were inconclusive.Post-procedural adverse events occurred in 33 patients(3.3%).Statistical analysis showed a difference between needle types(P=0.035)with a high sensitivity of FNB(97%).The analysis of the relationship between the MOSE-score and the final diagnosis showed a significant difference between the different scores of the MOSE(P<0.001).CONCLUSION MOSE is a simple method that allows endoscopists to increase needle passes to improve sample quality.There is significantly higher FNB sensitivity and cytopathology diagnostic yield with good MOSE cores.

Cheesy material on macroscopic on-site evaluation after endoscopic ultrasound-guided fine-needle biopsy:Don't miss the tuberculosis

Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)is an excellent investigation to diagnose pancreatic lesions and has shown high accuracy for its use in pathologic diagnosis.Recently,macroscopic on-site evaluation(MOSE)performed by an endoscopist was introduced as an alternative to rapid on-site cytologic evaluation to increase the diagnostic yield of EUS-FNB.The MOSE of the biopsy can estimate the adequacy of the sample directly by the macroscopic evaluation of the core tissue obtained from EUS-FNB.Isolated pancreatic tuberculosis is extremely rare and difficult to diagnose because of its non-specific signs and symptoms.Therefore,this challenging diagnosis is based on endoscopy,imaging,and the bacteriological and histological examination of tissue biopsies.This uncommon presentation of tuberculosis can be revealed as pancreatic mass mimicking cancer.EUS-FNB can be very useful in providing a valuable histopathological diagnosis.A calcified lesion with a cheesy core in MOSE must be suggestive of tuberculosis,leading to the request of the GeneXpert,which can detect Mycobacterium tuberculosis deoxyribonucleic acid and resistance to rifampicin.A decent diagnostic strategy is crucial to prevent unnecessary surgical resection and to supply conservative management with antitubercular therapy.

Development of a Novel Noise Reduction Algorithm for Smart Checkout RFID System in Retail Stores

This paper presents a smart checkout system designed to mitigate the issues of noise and errors present in the existing barcode and RFID-based systems used at retail stores’checkout counters.This is achieved by integrating a novel AI algorithm,called Improved Laser Simulator Logic(ILSL)into the RFID system.The enhanced RFID system was able to improve the accuracy of item identification,reduce noise interference,and streamline the overall checkout process.The potential of the systemfor noise detection and elimination was initially investigated through a simulation study usingMATLAB and ILSL algorithm.Subsequently,it was deployed in a small-scale environment to validate its real-world performance.Results show that RFID with the proposed new algorithm ILSL and AI basket is capable of accurately detecting the related itemswhile eliminating noise originating fromunrelated objects,achieving an accuracy rate of 88%.

融入中华优秀传统文化的Checkout time板块教学探索

译林版小学英语教材在每个单元的最后设置了Checkout time板块。Checkout time板块承载着整合、融合、延伸教学内容的使命。融入中华优秀传统文化的Checkout time板块教学,是围绕单元主题意义拓展传统文化知识,并将单元教学重难点融入文化教学的过程,能够促使学生灵活运用单元核心语言知识,在提升语言能力的同时,发展文化意识。具体可以从三方面出发:活动探究,丰富关于中华优秀传统文化的知识;问题引领,延展围绕中华优秀传统文化的讨论;资料引入,深化对于中华优秀传统文化的认识。

例谈基于Checkout time板块的英语写作教学策略

基于教材Checkout time板块开展小学英语写作教学要体现新课标精神,回应新课标要求“。基于教材文本进行扩写“”再构教材文本进行改写”和“超越教材文本进行创写”是基于Checkout time板块开展小学英语写作教学的有效策略,能实现学生写作能力的提升。

Rapid on-site evaluation of endoscopic-ultrasound-guided fine-needle aspiration diagnosis of pancreatic masses

Endoscopic ultrasound (EUS) has become an essential tool for the study of pancreatic diseases. Specifically, EUS plays a pivotal role evaluating patients with a known or suspected pancreatic mass. In this setting, differential diagnosis remains a clinical challenge. EUS-guided fine-needle aspiration (FNA) and fine-needle biopsy (FNB) have been proven to be safe and useful tools in this setting. EUS-guided FNA and FNB, by obtaining cytological and/or histological samples, are able to diagnose pancreatic lesions with high sensitivity and specificity. In this context, several methodological features, trying to increase the diagnostic yield of EUS-guided FNA and FNB, have been evaluated. In this review, we focus on the role of rapid on-site evaluation (ROSE). From data reported in the literature, ROSE may increase diagnostic yield of EUS-FNA specimens by 10%-30%, and thus, diagnostic accuracy. However, we should point out that many recent studies have reported adequacy rates of &#x0003e; 90% without ROSE, indicating that, perhaps, at high-volume centers, ROSE may not be indispensable to achieve excellent results. The use of ROSE can be considered important during the learning curve of EUS-FNA, and also in hospital with diagnostic accuracy rates &#x0003c; 90%.

Improved edge lightweight YOLOv4 and its application in on-site power system work

A“cloud-edge-end”collaborative system architecture is adopted for real-time security management of power system on-site work,and mobile edge computing equipment utilizes lightweight intelligent recognition algorithms for on-site risk assessment and alert.Owing to its lightweight and fast speed,YOLOv4-Tiny is often deployed on edge computing equipment for real-time video stream detection;however,its accuracy is relatively low.This study proposes an improved YOLOv4-Tiny algorithm based on attention mechanism and optimized training methods,achieving higher accuracy without compromising the speed.Specifically,a convolution block attention module branch is added to the backbone network to enhance the feature extraction capability and an efficient channel attention mechanism is added in the neck network to improve feature utilization.Moreover,three optimized training methods:transfer learning,mosaic data augmentation,and label smoothing are used to improve the training effect of this improved algorithm.Finally,an edge computing equipment experimental platform equipped with an NVIDIA Jetson Xavier NX chip is established and the newly developed algorithm is tested on it.According to the results,the speed of the improved YOLOv4-Tiny algorithm in detecting on-site dress code compliance datasets is 17.25 FPS,and the mean average precision(mAP)is increased from 70.89%to 85.03%.

Random Forests Algorithm Based Duplicate Detection in On-Site Programming Big Data Environment

On-site programming big data refers to the massive data generated in the process of software development with the characteristics of real-time,complexity and high-difficulty for processing.Therefore,data cleaning is essential for on-site programming big data.Duplicate data detection is an important step in data cleaning,which can save storage resources and enhance data consistency.Due to the insufficiency in traditional Sorted Neighborhood Method(SNM)and the difficulty of high-dimensional data detection,an optimized algorithm based on random forests with the dynamic and adaptive window size is proposed.The efficiency of the algorithm can be elevated by improving the method of the key-selection,reducing dimension of data set and using an adaptive variable size sliding window.Experimental results show that the improved SNM algorithm exhibits better performance and achieve higher accuracy.

New Explorations of Laboratory and On-site Testing of Broadband Seismographs in China

According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.

如何用好Checkout time板块开展单元复习

Checkout time板块通过形式多样的语言实践活动,考查学生对本单元所学语言知识的掌握情况。学生通过各种活动,将所学知识嵌入实践性的任务情境中,在知行协同、学思并行的学习过程中,完成知识技能的巩固拓展和主动建构。

Dynamical energy equipartition of the Toda model with additional on-site potentials

We study the dynamical energy equipartition properties in the integrable Toda model with additional uniform or disordered on-site energies by extensive numerical simulations. The total energy is initially equidistributed among some of the lowest frequency linear modes. For the Toda model with uniform on-site potentials, the energy spectrum keeps its profile nearly unchanged in a relatively short time scale. On a much longer time scale, the energies of tail modes increase slowly with time. Energy equipartition is far away from being attached in our studied time scale. For the Toda model with disordered on-site potentials, the energy transfers continuously to the high frequency modes and eventually towards energy equipartition. We further perform a systematic study of the equipartition time teq depending on the energy density ε and the nonlinear parameter α in the thermodynamic limit for the Toda model with disordered on-site potentials. We find teq∝ （1/ε）^a（1/α）^b, where b≈ 2a. The values of a and b are increased when increasing the strengths of disordered on-site potentials or decreasing the number of initially excited modes.

Increased Effluent Dosage Effects on On-Site Wastewater Treatment Systems of Differing Architecture Type

Approximately 20% of homes nationwide use an on-site treatment system as a form of household wastewater management. However, approximately 10% to 20% of on-site treatment systems malfunction each year, many of which have either failed or exceeded the soil’s long-term acceptance rate (LTAR), causing environmental and human health risks. The objective of this field study was to evaluate the effects of soil condition (e.g., wet and dry) and product architecture type [i.e., chamber, gravel-less-pipe (GLP), polystyrene-aggregate, and pipe-and-aggregate] on in-product solution storage and biomat thickness in a profile-limited soil in northwest Arkansas under increased loading rates and to estimate the LTAR for each product. During Phase I of this study (March 13 to October 4, 2013), effluent loading rates were approximately doubled, while rates were approximately quadrupled during Phase II (October 8, 2013 to May 29, 2014), from the maximum allowable loading rate for each product. The pipe-and-tire-chip, 46-cm-wide trench pipe-and-gravel, and the 25-cm diameter GLP products had the greatest (p < 0.001), while the 31-cm-width and the 5.4-m-long chambers had the lowest (p < 0.001) in-product solution storage during wet-soil conditions of Phase I monitoring. The 25-cm diameter GLP product had the greatest (p < 0.001), while the 61-cm-width, 5.4-m-long chamber had the lowest (p < 0.001) in-product solution storage during Phase II. Results of this study indicate that some alternative products may be able to effectively handle effluent loading rates in excess of those currently allowed by the State of Arkansas. Further research will be required to confirm these interpretations.

A rapid on-site calibration method for measuring dynamic envelope curve of high-speed trains

Dynamic envelope curve is a significant parameter to assess the running safety of high-speed trains.Up to now the method based on binocular stereo vision is the only way available to measure the dynamic envelope curve of a train,the speed of which is over200km/h.Nevertheless the method has two limitations,one is large field-〇f-view(FO V),the other is calibration time.Hence portable calibration equipment,easy-t〇-build target and rapid calibration algorithm are required to complete the calibration.In this paper,a new rapid on-site calibration method with large FOV based on binocular stereo vision is proposed.To address these issues,a light target has been designed,the rail coordinate system(RCS)is represented by40fixed retroreflective points on the target,and they are utilized to calibrate the parameters of two cameras.In addition,two cameras merely capture a single image of the target simultaneously,and the intrinsic and extrinsic parameters of the cameras can be calculated rapidly.To testify the proposed method,the experiments have been conducted and the results reveal that the accuracy can reach+1mm,which can meet the measurement requirement.

On-Site Calibration Method of Dosimeter Based on X-Ray Source

The real-time monitoring of environmental radiation dose for nuclear fa-cilities is an important part of safety, in order to guarantee the accuracy of the monitoring results regular calibration is necessary. Around nuclear facilities there are so many environmental dosimeters installed dispers-edly, because of its huge quantity, widely distributed, and in real-time monitoring state;it will cost lots of manpower and finance if it were tak-en to calibrate on standard laboratory;what’s more it will make the en-vironment out of control. To solve the problem of the measurement ac-curacy of the stationary gamma radiation dosimeter, an on-site calibra-tion method is proposed. The radioactive source is X-ray spectrum, and the dose reference instrument which has been calibrated by the national standard laboratory is a high pressure ionization. On-site calibration is divided into two parts;firstly the energy response experiment of dosim-eter for high and low energy is done in the laboratory, and the energy response curve is obtained combining with Monte Carlo simulation;sec-ondly experiment is carried out in the field of the measuring dosimeter, and the substitution method to calibrate the dosimeter is used;finally the calibration coefficient is gotten through energy curve correction. In order to verify the accuracy of on-site calibration method, the calibrated dosimeter is test in the standard laboratory and the error is 3.4%. The re-sult shows that the on-site calibration method using X-ray is feasible, and it can improves the accuracy of the measurement results of the stationary &gamma;-ray instrument;what’s more important is that it has great reference value for the radiation safety management and radiation environment evaluation.

An Experience of On-site PD Testing for Condition Monitoring of an 11 kV PILC Cable Insulation System

A cable circuit of a substation in the United Kingdom showed high level of PD activities during a survey using hand hold PD testing equipment. The authors were invited to carry out on-site PD testing experiment to further diagnose and locate the potential problem of the cable system. This paper presents the experience of the present authors carrying out the cable test. Following a brief introduction to the experiment equipments and physical connections, the paper analyses the data collected from the testing, including PD pulse shape analysis, frequency spectrum analysis and phase resolved PD pattern analysis. Associated with PD propagation direction identification, PD source diagnosis and localisation was made. Four different types of sensors, which were adapted during the testing, are shown to have different frequency bandwidths and performed differently. Aider comparing the parameters of the sensor and the PD signals detected by individual sensor, optimal PD monitoring bandwidth for cable system is suggested.

“教-学-评”一体化理念下的小学英语Checkout time板块教学例谈

译林版小学英语教材中,Checkout time板块是每个单元的综合语言运用板块,能通过多重学习任务帮助学生巩固本单元的重点知识。但在实际教学中,部分教师对Checkout time板块的讲解不到位,存在零散活动拼凑、课型定位不明等现象,无法彰显全面复习及综合运用的板块功能。文章结合译林版小学英语6A“Unit3 Holiday fun”中Checkout time板块的教学,基于“教—学—评”一体化理念,主要分析了Checkout time板块教学中常见的误区,并对Checkout time板块的教学实践进行了探究,以供参考。

小学英语Checkout time板块教学的有效策略——以六年级上册Unit 6 Keep our city clean为例

译林版小学《英语》Checkout time板块教学是单元教学的重要课时之一。文章通过对Checkout time板块功能的解读以及教学现状的分析,结合译林版小学英语六年级上册Unit 6 Keep our city clean第四课时的教学案例,提出了Checkout time板块的五个教学策略:巧设开端,打造轻快的课堂氛围;结合文本,建立真实的语言情境;层层递进,开展高效的教学活动;融合绘本,拓展学生的知识面;体验情感,发挥课堂的育人价值。

基于学习活动观的小学英语Checkout Time板块设计的实践研究

解读英语学习活动观的内涵.阐述基于学习活动观开展小学英语教学的意义.从译林版小学英语教材的Checkout Time板块出发,结合教学实例,探索基于学习活动观的Checkout Time板块的设计,提出开展提问,引导学生思考理解;创设情境,提高学生口语能力;提出质疑,激发学生挖掘内涵;文本迁移,培养学生习作水平等措施.认为学习活动观与Checkout Time板块的有效结合,可以通过各种丰富的学习活动促进学生理解、实践、创新等能力的发展.

机械设备电气故障自动检测系统优化设计

当前系统不能有效消除干扰信号,数据采集精度低,影响机械设备电气故障诊断,为此设计一种新的机械设备电气故障自动检测系统。采集电气故障检测数据,提取电气故障特征,根据特征判断机械设备的电气故障原因,计算纠正参数完善电气故障检测结果,线性处理电压检测曲线,完成机械设备电气故障检测。测试结果验证,所提系统能够有效消除干扰信号,保证整体功能运行稳定,相对于对比系统,响应时间更短,数据采集精度得到明显改善。