A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Cheesy material on macroscopic on-site evaluation after endoscopic ultrasound-guided fine-needle biopsy:Don't miss the tuberculosis

Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)is an excellent investigation to diagnose pancreatic lesions and has shown high accuracy for its use in pathologic diagnosis.Recently,macroscopic on-site evaluation(MOSE)performed by an endoscopist was introduced as an alternative to rapid on-site cytologic evaluation to increase the diagnostic yield of EUS-FNB.The MOSE of the biopsy can estimate the adequacy of the sample directly by the macroscopic evaluation of the core tissue obtained from EUS-FNB.Isolated pancreatic tuberculosis is extremely rare and difficult to diagnose because of its non-specific signs and symptoms.Therefore,this challenging diagnosis is based on endoscopy,imaging,and the bacteriological and histological examination of tissue biopsies.This uncommon presentation of tuberculosis can be revealed as pancreatic mass mimicking cancer.EUS-FNB can be very useful in providing a valuable histopathological diagnosis.A calcified lesion with a cheesy core in MOSE must be suggestive of tuberculosis,leading to the request of the GeneXpert,which can detect Mycobacterium tuberculosis deoxyribonucleic acid and resistance to rifampicin.A decent diagnostic strategy is crucial to prevent unnecessary surgical resection and to supply conservative management with antitubercular therapy.

Development of a Novel Noise Reduction Algorithm for Smart Checkout RFID System in Retail Stores

This paper presents a smart checkout system designed to mitigate the issues of noise and errors present in the existing barcode and RFID-based systems used at retail stores’checkout counters.This is achieved by integrating a novel AI algorithm,called Improved Laser Simulator Logic(ILSL)into the RFID system.The enhanced RFID system was able to improve the accuracy of item identification,reduce noise interference,and streamline the overall checkout process.The potential of the systemfor noise detection and elimination was initially investigated through a simulation study usingMATLAB and ILSL algorithm.Subsequently,it was deployed in a small-scale environment to validate its real-world performance.Results show that RFID with the proposed new algorithm ILSL and AI basket is capable of accurately detecting the related itemswhile eliminating noise originating fromunrelated objects,achieving an accuracy rate of 88%.

Improved edge lightweight YOLOv4 and its application in on-site power system work

A“cloud-edge-end”collaborative system architecture is adopted for real-time security management of power system on-site work,and mobile edge computing equipment utilizes lightweight intelligent recognition algorithms for on-site risk assessment and alert.Owing to its lightweight and fast speed,YOLOv4-Tiny is often deployed on edge computing equipment for real-time video stream detection;however,its accuracy is relatively low.This study proposes an improved YOLOv4-Tiny algorithm based on attention mechanism and optimized training methods,achieving higher accuracy without compromising the speed.Specifically,a convolution block attention module branch is added to the backbone network to enhance the feature extraction capability and an efficient channel attention mechanism is added in the neck network to improve feature utilization.Moreover,three optimized training methods:transfer learning,mosaic data augmentation,and label smoothing are used to improve the training effect of this improved algorithm.Finally,an edge computing equipment experimental platform equipped with an NVIDIA Jetson Xavier NX chip is established and the newly developed algorithm is tested on it.According to the results,the speed of the improved YOLOv4-Tiny algorithm in detecting on-site dress code compliance datasets is 17.25 FPS,and the mean average precision(mAP)is increased from 70.89%to 85.03%.

Random Forests Algorithm Based Duplicate Detection in On-Site Programming Big Data Environment

On-site programming big data refers to the massive data generated in the process of software development with the characteristics of real-time,complexity and high-difficulty for processing.Therefore,data cleaning is essential for on-site programming big data.Duplicate data detection is an important step in data cleaning,which can save storage resources and enhance data consistency.Due to the insufficiency in traditional Sorted Neighborhood Method(SNM)and the difficulty of high-dimensional data detection,an optimized algorithm based on random forests with the dynamic and adaptive window size is proposed.The efficiency of the algorithm can be elevated by improving the method of the key-selection,reducing dimension of data set and using an adaptive variable size sliding window.Experimental results show that the improved SNM algorithm exhibits better performance and achieve higher accuracy.

New Explorations of Laboratory and On-site Testing of Broadband Seismographs in China

According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.

Dynamical energy equipartition of the Toda model with additional on-site potentials

We study the dynamical energy equipartition properties in the integrable Toda model with additional uniform or disordered on-site energies by extensive numerical simulations. The total energy is initially equidistributed among some of the lowest frequency linear modes. For the Toda model with uniform on-site potentials, the energy spectrum keeps its profile nearly unchanged in a relatively short time scale. On a much longer time scale, the energies of tail modes increase slowly with time. Energy equipartition is far away from being attached in our studied time scale. For the Toda model with disordered on-site potentials, the energy transfers continuously to the high frequency modes and eventually towards energy equipartition. We further perform a systematic study of the equipartition time teq depending on the energy density ε and the nonlinear parameter α in the thermodynamic limit for the Toda model with disordered on-site potentials. We find teq∝ （1/ε）^a（1/α）^b, where b≈ 2a. The values of a and b are increased when increasing the strengths of disordered on-site potentials or decreasing the number of initially excited modes.

如何用好Checkout time板块开展单元复习

Checkout time板块通过形式多样的语言实践活动,考查学生对本单元所学语言知识的掌握情况。学生通过各种活动,将所学知识嵌入实践性的任务情境中,在知行协同、学思并行的学习过程中,完成知识技能的巩固拓展和主动建构。

Increased Effluent Dosage Effects on On-Site Wastewater Treatment Systems of Differing Architecture Type

Approximately 20% of homes nationwide use an on-site treatment system as a form of household wastewater management. However, approximately 10% to 20% of on-site treatment systems malfunction each year, many of which have either failed or exceeded the soil’s long-term acceptance rate (LTAR), causing environmental and human health risks. The objective of this field study was to evaluate the effects of soil condition (e.g., wet and dry) and product architecture type [i.e., chamber, gravel-less-pipe (GLP), polystyrene-aggregate, and pipe-and-aggregate] on in-product solution storage and biomat thickness in a profile-limited soil in northwest Arkansas under increased loading rates and to estimate the LTAR for each product. During Phase I of this study (March 13 to October 4, 2013), effluent loading rates were approximately doubled, while rates were approximately quadrupled during Phase II (October 8, 2013 to May 29, 2014), from the maximum allowable loading rate for each product. The pipe-and-tire-chip, 46-cm-wide trench pipe-and-gravel, and the 25-cm diameter GLP products had the greatest (p < 0.001), while the 31-cm-width and the 5.4-m-long chambers had the lowest (p < 0.001) in-product solution storage during wet-soil conditions of Phase I monitoring. The 25-cm diameter GLP product had the greatest (p < 0.001), while the 61-cm-width, 5.4-m-long chamber had the lowest (p < 0.001) in-product solution storage during Phase II. Results of this study indicate that some alternative products may be able to effectively handle effluent loading rates in excess of those currently allowed by the State of Arkansas. Further research will be required to confirm these interpretations.

On-Site Calibration Method of Dosimeter Based on X-Ray Source

The real-time monitoring of environmental radiation dose for nuclear fa-cilities is an important part of safety, in order to guarantee the accuracy of the monitoring results regular calibration is necessary. Around nuclear facilities there are so many environmental dosimeters installed dispers-edly, because of its huge quantity, widely distributed, and in real-time monitoring state;it will cost lots of manpower and finance if it were tak-en to calibrate on standard laboratory;what’s more it will make the en-vironment out of control. To solve the problem of the measurement ac-curacy of the stationary gamma radiation dosimeter, an on-site calibra-tion method is proposed. The radioactive source is X-ray spectrum, and the dose reference instrument which has been calibrated by the national standard laboratory is a high pressure ionization. On-site calibration is divided into two parts;firstly the energy response experiment of dosim-eter for high and low energy is done in the laboratory, and the energy response curve is obtained combining with Monte Carlo simulation;sec-ondly experiment is carried out in the field of the measuring dosimeter, and the substitution method to calibrate the dosimeter is used;finally the calibration coefficient is gotten through energy curve correction. In order to verify the accuracy of on-site calibration method, the calibrated dosimeter is test in the standard laboratory and the error is 3.4%. The re-sult shows that the on-site calibration method using X-ray is feasible, and it can improves the accuracy of the measurement results of the stationary &gamma;-ray instrument;what’s more important is that it has great reference value for the radiation safety management and radiation environment evaluation.

“教-学-评”一体化理念下的小学英语Checkout time板块教学例谈

译林版小学英语教材中,Checkout time板块是每个单元的综合语言运用板块,能通过多重学习任务帮助学生巩固本单元的重点知识。但在实际教学中,部分教师对Checkout time板块的讲解不到位,存在零散活动拼凑、课型定位不明等现象,无法彰显全面复习及综合运用的板块功能。文章结合译林版小学英语6A“Unit3 Holiday fun”中Checkout time板块的教学,基于“教—学—评”一体化理念,主要分析了Checkout time板块教学中常见的误区,并对Checkout time板块的教学实践进行了探究,以供参考。

基于学习活动观的小学英语Checkout Time板块设计的实践研究

解读英语学习活动观的内涵.阐述基于学习活动观开展小学英语教学的意义.从译林版小学英语教材的Checkout Time板块出发,结合教学实例,探索基于学习活动观的Checkout Time板块的设计,提出开展提问,引导学生思考理解;创设情境,提高学生口语能力;提出质疑,激发学生挖掘内涵;文本迁移,培养学生习作水平等措施.认为学习活动观与Checkout Time板块的有效结合,可以通过各种丰富的学习活动促进学生理解、实践、创新等能力的发展.

机械设备电气故障自动检测系统优化设计

当前系统不能有效消除干扰信号,数据采集精度低,影响机械设备电气故障诊断,为此设计一种新的机械设备电气故障自动检测系统。采集电气故障检测数据,提取电气故障特征,根据特征判断机械设备的电气故障原因,计算纠正参数完善电气故障检测结果,线性处理电压检测曲线,完成机械设备电气故障检测。测试结果验证,所提系统能够有效消除干扰信号,保证整体功能运行稳定,相对于对比系统,响应时间更短,数据采集精度得到明显改善。

妙用教材的“边角料”——试析checkout time板块和grammar time板块的教学

在译林新版《英语》的教学中,注重story time、cartoon time,轻视checkout time、grammar time的现象比比皆是,后者成了教学中的'边角料'。作为教材的组成部分,checkout time能为学生语用能力的提升夯实基础,grammar time则能为语言的生成增添活力。教师应挖掘教材,通过多样化的语言活动,巧妙地处理教材中的'边角料'。

Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1

Human bocavirus(HBoV)1 is considered an important pathogen that mainly affects infants aged 6–24 months,but preventing viral transmission in resource-limited regions through rapid and affordable on-site diagnosis of individuals with early infection of HBoV1 remains somewhat challenging.Herein,we present a novel faster,lower cost,reliable method for the detection of HBoV1,which integrates a recombinase polymerase amplification(RPA)assay with the CRISPR/Cas12a system,designated the RPA-Cas12a-fluorescence assay.The RPA-Cas12a-fluorescence system can specifically detect target gene levels as low as 0.5 copies of HBoV1 plasmid DNA per microliter within 40 min at 37℃without the need for sophisticated instruments.The method also demonstrates excellent specificity without cross-reactivity to non-target pathogens.Furthermore,the method was appraised using 28 clinical samples,and displayed high accuracy with positive and negative predictive agreement of 90.9%and 100%,respectively.Therefore,our proposed rapid and sensitive HBoV1 detection method,the RPA-Cas12a-fluorescence assay,shows promising potential for early on-site diagnosis of HBoV1 infection in the fields of public health and health care.The established RPA-Cas12a-fluorescence assay is rapid and reliable method for human bocavirus 1 detection.The RPA-Cas12a-fluorescence assay can be completed within 40 min with robust specificity and sensitivity of 0.5 copies/μl.

Simulation of fire emergency evacuation in a large-passenger-flow subway station based on the on-site measured data of Shenzhen Metro

Because of its large capacity,high efficiency and energy savings,the subway has gradually become the primary mode of transportation for citizens.A high density of passengers exists within a large-passenger-flow subway station,and the number of casualties and injuries during a fire emergency is substantial.In this paper,Pathfinder software and on-site measured data of Pingzhou station in Shenzhen(China)were utilized to simulate a fire emergency evacuation in a large-passenger-flow subway station.The Required Safe Egress Time(RSET),number of passengers and flow rates of stairs and escalators were analysed for three fire evacuation scenarios:train fire,platform fire and hall fire.The evacuation time of the train fire,which was 1173 s,was the longest,and 3621 occupants needed to evacuate when the train was fully loaded.Occupants could not complete the evacuation within 6 mins in all three fire evacuation scenarios,which does not meet the current standard requirements and codes.By changing the number of passengers and the number of stairs for evacuation,the flow rate capacity and evacuation time were explored,which have reference values for safety management and emergency evacuation plan optimization during peak hours of subway operation.

谈Checkout time板块的教学策略——以译林新版《英语》五上Unit 8 At Christmas为例

译林新版小学《英语》教材中的Checkout time是一个综合检测板块,它通过形式多样的语言实践活动,考查学生对整个单元所学语言知识的掌握情况,提高其听、说、读、写的能力,内化其语言知识,发展其语言技能。然而,据笔者观察,到目前为止,这一板块的教学尚未引起广大教师的重视。

小学高年级英语checkout time单元复习课的问题与对策

译林版小学《英语》高年级checkout time单元复习课是非常重要的教学内容,主要用于考察学生对于单元知识的掌握情况,同时提升学生的综合语用能力。但是在日常教学实践中,仍然会出现目标定位不准、活动层次混乱、写作指导低效、主题意义缺失的问题。因此,结合教学实例,探讨了融合多元目标、主线串联任务、挖掘文本资源以及凸显主题意义等实施策略。

基于工程教育认证的“双化三验”课程设计模式探究——以水污染控制工程课程设计为例

为了培养满足工程教育认证要求的专业人才,文章以水污染控制工程课程设计为例,提出了“双化三验”课程设计模式,即通过开题、中期检查和答辩三个环节,针对设计中存在的不标准和不规范问题,对学生进行指导,以确保课程设计的标准化和规范化开展。该模式的实施收到了一定的成效,可为其他专业课程设计的教学提供参考。

做好加减法 提升板块效能——谈译林版《英语》Checkout Time板块教学的有效性

Checkout Time板块是教材中的重要板块,承担着对语言知识和技能的综合检测的使命。有效提升教学板块的有效性是广大英语教师不断探索的课题。文章结合具体的课堂实践案例谈谈如何有效根据单元教学内容和目标,从语言巩固和运用的角度做好Checkout Time板块的加减法,从而有效提高Checkout Time教学的有效性,最大限度地发挥Checkout Time板块的教学效能。