A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Cheesy material on macroscopic on-site evaluation after endoscopic ultrasound-guided fine-needle biopsy:Don't miss the tuberculosis

Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)is an excellent investigation to diagnose pancreatic lesions and has shown high accuracy for its use in pathologic diagnosis.Recently,macroscopic on-site evaluation(MOSE)performed by an endoscopist was introduced as an alternative to rapid on-site cytologic evaluation to increase the diagnostic yield of EUS-FNB.The MOSE of the biopsy can estimate the adequacy of the sample directly by the macroscopic evaluation of the core tissue obtained from EUS-FNB.Isolated pancreatic tuberculosis is extremely rare and difficult to diagnose because of its non-specific signs and symptoms.Therefore,this challenging diagnosis is based on endoscopy,imaging,and the bacteriological and histological examination of tissue biopsies.This uncommon presentation of tuberculosis can be revealed as pancreatic mass mimicking cancer.EUS-FNB can be very useful in providing a valuable histopathological diagnosis.A calcified lesion with a cheesy core in MOSE must be suggestive of tuberculosis,leading to the request of the GeneXpert,which can detect Mycobacterium tuberculosis deoxyribonucleic acid and resistance to rifampicin.A decent diagnostic strategy is crucial to prevent unnecessary surgical resection and to supply conservative management with antitubercular therapy.

Rapid on-site evaluation of endoscopic-ultrasound-guided fine-needle aspiration diagnosis of pancreatic masses

Endoscopic ultrasound (EUS) has become an essential tool for the study of pancreatic diseases. Specifically, EUS plays a pivotal role evaluating patients with a known or suspected pancreatic mass. In this setting, differential diagnosis remains a clinical challenge. EUS-guided fine-needle aspiration (FNA) and fine-needle biopsy (FNB) have been proven to be safe and useful tools in this setting. EUS-guided FNA and FNB, by obtaining cytological and/or histological samples, are able to diagnose pancreatic lesions with high sensitivity and specificity. In this context, several methodological features, trying to increase the diagnostic yield of EUS-guided FNA and FNB, have been evaluated. In this review, we focus on the role of rapid on-site evaluation (ROSE). From data reported in the literature, ROSE may increase diagnostic yield of EUS-FNA specimens by 10%-30%, and thus, diagnostic accuracy. However, we should point out that many recent studies have reported adequacy rates of > 90% without ROSE, indicating that, perhaps, at high-volume centers, ROSE may not be indispensable to achieve excellent results. The use of ROSE can be considered important during the learning curve of EUS-FNA, and also in hospital with diagnostic accuracy rates < 90%.

Improved edge lightweight YOLOv4 and its application in on-site power system work

A“cloud-edge-end”collaborative system architecture is adopted for real-time security management of power system on-site work,and mobile edge computing equipment utilizes lightweight intelligent recognition algorithms for on-site risk assessment and alert.Owing to its lightweight and fast speed,YOLOv4-Tiny is often deployed on edge computing equipment for real-time video stream detection;however,its accuracy is relatively low.This study proposes an improved YOLOv4-Tiny algorithm based on attention mechanism and optimized training methods,achieving higher accuracy without compromising the speed.Specifically,a convolution block attention module branch is added to the backbone network to enhance the feature extraction capability and an efficient channel attention mechanism is added in the neck network to improve feature utilization.Moreover,three optimized training methods:transfer learning,mosaic data augmentation,and label smoothing are used to improve the training effect of this improved algorithm.Finally,an edge computing equipment experimental platform equipped with an NVIDIA Jetson Xavier NX chip is established and the newly developed algorithm is tested on it.According to the results,the speed of the improved YOLOv4-Tiny algorithm in detecting on-site dress code compliance datasets is 17.25 FPS,and the mean average precision(mAP)is increased from 70.89%to 85.03%.

Random Forests Algorithm Based Duplicate Detection in On-Site Programming Big Data Environment

On-site programming big data refers to the massive data generated in the process of software development with the characteristics of real-time,complexity and high-difficulty for processing.Therefore,data cleaning is essential for on-site programming big data.Duplicate data detection is an important step in data cleaning,which can save storage resources and enhance data consistency.Due to the insufficiency in traditional Sorted Neighborhood Method(SNM)and the difficulty of high-dimensional data detection,an optimized algorithm based on random forests with the dynamic and adaptive window size is proposed.The efficiency of the algorithm can be elevated by improving the method of the key-selection,reducing dimension of data set and using an adaptive variable size sliding window.Experimental results show that the improved SNM algorithm exhibits better performance and achieve higher accuracy.

New Explorations of Laboratory and On-site Testing of Broadband Seismographs in China

According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.

Dynamical energy equipartition of the Toda model with additional on-site potentials

We study the dynamical energy equipartition properties in the integrable Toda model with additional uniform or disordered on-site energies by extensive numerical simulations. The total energy is initially equidistributed among some of the lowest frequency linear modes. For the Toda model with uniform on-site potentials, the energy spectrum keeps its profile nearly unchanged in a relatively short time scale. On a much longer time scale, the energies of tail modes increase slowly with time. Energy equipartition is far away from being attached in our studied time scale. For the Toda model with disordered on-site potentials, the energy transfers continuously to the high frequency modes and eventually towards energy equipartition. We further perform a systematic study of the equipartition time teq depending on the energy density ε and the nonlinear parameter α in the thermodynamic limit for the Toda model with disordered on-site potentials. We find teq∝ （1/ε）^a（1/α）^b, where b≈ 2a. The values of a and b are increased when increasing the strengths of disordered on-site potentials or decreasing the number of initially excited modes.

Increased Effluent Dosage Effects on On-Site Wastewater Treatment Systems of Differing Architecture Type

Approximately 20% of homes nationwide use an on-site treatment system as a form of household wastewater management. However, approximately 10% to 20% of on-site treatment systems malfunction each year, many of which have either failed or exceeded the soil’s long-term acceptance rate (LTAR), causing environmental and human health risks. The objective of this field study was to evaluate the effects of soil condition (e.g., wet and dry) and product architecture type [i.e., chamber, gravel-less-pipe (GLP), polystyrene-aggregate, and pipe-and-aggregate] on in-product solution storage and biomat thickness in a profile-limited soil in northwest Arkansas under increased loading rates and to estimate the LTAR for each product. During Phase I of this study (March 13 to October 4, 2013), effluent loading rates were approximately doubled, while rates were approximately quadrupled during Phase II (October 8, 2013 to May 29, 2014), from the maximum allowable loading rate for each product. The pipe-and-tire-chip, 46-cm-wide trench pipe-and-gravel, and the 25-cm diameter GLP products had the greatest (p < 0.001), while the 31-cm-width and the 5.4-m-long chambers had the lowest (p < 0.001) in-product solution storage during wet-soil conditions of Phase I monitoring. The 25-cm diameter GLP product had the greatest (p < 0.001), while the 61-cm-width, 5.4-m-long chamber had the lowest (p < 0.001) in-product solution storage during Phase II. Results of this study indicate that some alternative products may be able to effectively handle effluent loading rates in excess of those currently allowed by the State of Arkansas. Further research will be required to confirm these interpretations.

A rapid on-site calibration method for measuring dynamic envelope curve of high-speed trains

Dynamic envelope curve is a significant parameter to assess the running safety of high-speed trains.Up to now the method based on binocular stereo vision is the only way available to measure the dynamic envelope curve of a train,the speed of which is over200km/h.Nevertheless the method has two limitations,one is large field-〇f-view(FO V),the other is calibration time.Hence portable calibration equipment,easy-t〇-build target and rapid calibration algorithm are required to complete the calibration.In this paper,a new rapid on-site calibration method with large FOV based on binocular stereo vision is proposed.To address these issues,a light target has been designed,the rail coordinate system(RCS)is represented by40fixed retroreflective points on the target,and they are utilized to calibrate the parameters of two cameras.In addition,two cameras merely capture a single image of the target simultaneously,and the intrinsic and extrinsic parameters of the cameras can be calculated rapidly.To testify the proposed method,the experiments have been conducted and the results reveal that the accuracy can reach+1mm,which can meet the measurement requirement.

On-Site Calibration Method of Dosimeter Based on X-Ray Source

The real-time monitoring of environmental radiation dose for nuclear fa-cilities is an important part of safety, in order to guarantee the accuracy of the monitoring results regular calibration is necessary. Around nuclear facilities there are so many environmental dosimeters installed dispers-edly, because of its huge quantity, widely distributed, and in real-time monitoring state;it will cost lots of manpower and finance if it were tak-en to calibrate on standard laboratory;what’s more it will make the en-vironment out of control. To solve the problem of the measurement ac-curacy of the stationary gamma radiation dosimeter, an on-site calibra-tion method is proposed. The radioactive source is X-ray spectrum, and the dose reference instrument which has been calibrated by the national standard laboratory is a high pressure ionization. On-site calibration is divided into two parts;firstly the energy response experiment of dosim-eter for high and low energy is done in the laboratory, and the energy response curve is obtained combining with Monte Carlo simulation;sec-ondly experiment is carried out in the field of the measuring dosimeter, and the substitution method to calibrate the dosimeter is used;finally the calibration coefficient is gotten through energy curve correction. In order to verify the accuracy of on-site calibration method, the calibrated dosimeter is test in the standard laboratory and the error is 3.4%. The re-sult shows that the on-site calibration method using X-ray is feasible, and it can improves the accuracy of the measurement results of the stationary &gamma;-ray instrument;what’s more important is that it has great reference value for the radiation safety management and radiation environment evaluation.

An Experience of On-site PD Testing for Condition Monitoring of an 11 kV PILC Cable Insulation System

A cable circuit of a substation in the United Kingdom showed high level of PD activities during a survey using hand hold PD testing equipment. The authors were invited to carry out on-site PD testing experiment to further diagnose and locate the potential problem of the cable system. This paper presents the experience of the present authors carrying out the cable test. Following a brief introduction to the experiment equipments and physical connections, the paper analyses the data collected from the testing, including PD pulse shape analysis, frequency spectrum analysis and phase resolved PD pattern analysis. Associated with PD propagation direction identification, PD source diagnosis and localisation was made. Four different types of sensors, which were adapted during the testing, are shown to have different frequency bandwidths and performed differently. Aider comparing the parameters of the sensor and the PD signals detected by individual sensor, optimal PD monitoring bandwidth for cable system is suggested.

Development of RPA-Cas12a-fluorescence assay for rapid and reliable detection of human bocavirus 1

Human bocavirus(HBoV)1 is considered an important pathogen that mainly affects infants aged 6–24 months,but preventing viral transmission in resource-limited regions through rapid and affordable on-site diagnosis of individuals with early infection of HBoV1 remains somewhat challenging.Herein,we present a novel faster,lower cost,reliable method for the detection of HBoV1,which integrates a recombinase polymerase amplification(RPA)assay with the CRISPR/Cas12a system,designated the RPA-Cas12a-fluorescence assay.The RPA-Cas12a-fluorescence system can specifically detect target gene levels as low as 0.5 copies of HBoV1 plasmid DNA per microliter within 40 min at 37℃without the need for sophisticated instruments.The method also demonstrates excellent specificity without cross-reactivity to non-target pathogens.Furthermore,the method was appraised using 28 clinical samples,and displayed high accuracy with positive and negative predictive agreement of 90.9%and 100%,respectively.Therefore,our proposed rapid and sensitive HBoV1 detection method,the RPA-Cas12a-fluorescence assay,shows promising potential for early on-site diagnosis of HBoV1 infection in the fields of public health and health care.The established RPA-Cas12a-fluorescence assay is rapid and reliable method for human bocavirus 1 detection.The RPA-Cas12a-fluorescence assay can be completed within 40 min with robust specificity and sensitivity of 0.5 copies/μl.

Simulation of fire emergency evacuation in a large-passenger-flow subway station based on the on-site measured data of Shenzhen Metro

Because of its large capacity,high efficiency and energy savings,the subway has gradually become the primary mode of transportation for citizens.A high density of passengers exists within a large-passenger-flow subway station,and the number of casualties and injuries during a fire emergency is substantial.In this paper,Pathfinder software and on-site measured data of Pingzhou station in Shenzhen(China)were utilized to simulate a fire emergency evacuation in a large-passenger-flow subway station.The Required Safe Egress Time(RSET),number of passengers and flow rates of stairs and escalators were analysed for three fire evacuation scenarios:train fire,platform fire and hall fire.The evacuation time of the train fire,which was 1173 s,was the longest,and 3621 occupants needed to evacuate when the train was fully loaded.Occupants could not complete the evacuation within 6 mins in all three fire evacuation scenarios,which does not meet the current standard requirements and codes.By changing the number of passengers and the number of stairs for evacuation,the flow rate capacity and evacuation time were explored,which have reference values for safety management and emergency evacuation plan optimization during peak hours of subway operation.

Research advances in enhanced coal seam gas extraction by controllable shock wave fracturing

With the continuous increase of mining in depth,the gas extraction faces the challenges of low permeability,great ground stress,high temperature and large gas pressure in coal seam.The controllable shock wave(CSW),as a new method for enhancing permeability of coal seam to improve gas extraction,features in the advantages of high efficiency,eco-friendly,and low cost.In order to better utilize the CSW into gas extraction in coal mine,the mechanism and feasibility of CSW enhanced extraction need to be studied.In this paper,the basic principles,the experimental tests,the mathematical models,and the on-site tests of CSW fracturing coal seams are reviewed,thereby its future research directions are provided.Based on the different media between electrodes,the CSW can be divided into three categories:hydraulic effect,wire explosion and excitation of energetic materials by detonating wire.During the process of propagation and attenuation of the high-energy shock wave in coal,the shock wave and bubble pulsation work together to produce an enhanced permeability effect on the coal seam.The stronger the strength of the CSW is,the more cracks created in the coal is,and the greater the length,width and area of the cracks being.The repeated shock on the coal seam is conducive to the formation of complex network fracture system as well as the reduction of coal seam strength,but excessive shock frequency will also damage the coal structure,resulting in the limited effect of the enhanced gas extraction.Under the influence of ground stress,the crack propagation in coal seam will be restrained.The difference of horizontal principal stress has a significant impact on the shape,propagation direction and connectivity of the CSW induced cracks.The permeability enhancement effect of CSW is affected by the breakage degree of coal seam.The shock wave is absorbed by the broken coal,which may hinder the propagation of CSW,resulting in a poor effect of permeability enhancement.When arranging two adjacent boreholes for CSW permeability enhancement test,the spacing of boreholes should not be too close,which may lead to negative pressure mutual pulling in the early stage of drainage.At present,the accurate method for effectively predicting the CSW permeability enhanced range should be further investigated.

非均衡治理理论视角下改革失败的实例与防范策略

改革失败有其深刻内涵、诸多特征及明显危害,其根本成因是:改革过程中未能切实借助非均衡手段打破“低水平锁定”状态。具体成因有:事物的形式与实质截然不同;受“治理惯性”影响的“旧人”实施改革举措;并未真正做到因需制宜。防范改革失败的根本策略是:促使多元治理主体在改革过程中基于“执政党/领导者—政府—市场—社会”金字塔型治理结构有效运用现代治理技术、因需制宜地实施变革,基于非均衡手段有效打破“低水平锁定”状态。微观策略是在改革过程中:实现事物的形式与实质相同;切实破旧立新,有效打破“低水平锁定”;实现因需制宜。

基于ZigBee的变电站锁态控制系统研究

随着智能电网的发展,变电站智能化水平不断提高,对设备锁态控制提出更高要求。文章针对当前变电站锁态控制存在的问题,提出一种基于ZigBee无线通信技术的智能锁态控制系统。系统集成CC2530芯片和Z-Stack协议栈,实现锁态信号的实时采集、可靠传输以及智能化控制。通过OPNET仿真实验,验证系统的可行性和有效性,为变电站智能化升级改造提供新思路。

浅析配电主站+电压时间型自愈模式后备策略方法

配电自动化在10 kV线路故障隔离、转供电应用逐渐加强,配电主站将主站与电压-时间协同型自愈策略广泛应用于10 kV线路故障的定位、隔离,实现自愈转供电,提高了供电可靠性;当配电主站采集配电自动化开关闭锁信号缺失、故障边界不确定时,自愈策略自动终止,则造成变电站出线开关和配电线路上的自动化开关重复动作或故障区段进一步扩大。后备策略利用现有的调度自动化、配网自动化等系统数据信息,在主站自愈策略实施终止后启用后备策略进行综合分析快速缩小配电线路故障范围、隔离故障区段。

局部通风电机智能保护器优化设计

针对局部通风电机保护系统存在的智能化程度低、灵敏度不高、保护动作延时大的问题,设计了一种基于TMS320F28335 DSP处理器的局部通风电机智能保护器优化方案。在分析通风电机智能保护器功能需求的基础上,重点阐述了硬件、软件设计思路与方法,并完成了试验验证。试验结果表明:优化后的局部通风电机智能保护器智能化程度高、灵敏度高、保护动作延时小,能够实现局部通风电机预期保护功能,有助于提升工作面安全性和作业效率。

一起励磁涌流闭锁导致的配电自动化开关异常动作案例分析

立足于当前云南电网配电自动化实用化背景,结合系统内一起配电自动化开关异常动作案例,针对配电自动化开关励磁涌流闭锁功能错误触发开展分析并给出优化建议。首先,介绍了线路参数及保护配置情况,奠定后续分析基础。之后,基于SOE信息复现开关动作过程,明确开关异常动作情况。最后,结合故障录波信息阐述配电自动化开关异动原因,并给出优化建议。

LOCKOUT继电器在特高压直流工程二次回路中的应用研究

由于巴西当地电网运行维护习惯不同,巴西美丽山二期±800 kV特高压直流工程交流系统二次回路中的LOCKOUT继电器与中国工程的应用和配置原则存在较大的差别。详细分析和比较了巴西与中国特高压换流站交流系统典型的断路器操作回路的差异性,提出了适用于巴西当地电网运行维护要求的特高压换流站交流系统二次回路中LOCKOUT继电器的配置原则。并以欣古换流站交流滤波器小组断路器的操作回路为例,详细介绍了LOCKOUT继电器的启动和闭锁逻辑。所提出的LOCKOUT继电器的配置原则有利于更好地适应巴西当地的电网建设,为特高压直流输电技术走出国门、服务世界提供了强有力的技术支撑。