Porcine epidemic diarrhea virus(PEDV),as the main causative pathogen of viral diarrhea in pigs,has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to t...Porcine epidemic diarrhea virus(PEDV),as the main causative pathogen of viral diarrhea in pigs,has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to the swine industry.Rapid diagnosis methods are essential for preventing outbreaks and transmission of this disease.In this study,a paper-based lateral flow immunoassay for the rapid diagnosis of PEDV in swine fecal samples was developed using stable color-rich latex beads as the label.Under optimal conditions,the newly developed latex bead-based lateral flow immunoassay(LBs-LFIA)attained a limit of detection(LOD)as low as 10^(3.60) TCID_(50)/mL and no cross-reactivity with other related swine viruses.To solve swine feces impurity interference,by adding a filtration unit design of LFIA without an additional pretreatment procedure,the LBs-LFIA gave good agreement(92.59%)with RT-PCR results in the analysis of clinical swine fecal samples{n=108),which was more accurate than previously reported colloidal gold LFIA(74.07%)and fluorescent LFIA(86.67%).Moreover,LBs-LFIA showed sufficient accuracy(coefficient of variance[CV]<15%)and stable(room temperature storage life>56 days)performance for PEDV detection,which is promising for on-site analysis and user-driven testing in pig production system.展开更多
Organophosphate esters（OPEs）, used as flame retardants and plasticizers, are widely present in environmental waters. Development of accurate determination methods for trace OPEs in water is urgent for understanding ...Organophosphate esters（OPEs）, used as flame retardants and plasticizers, are widely present in environmental waters. Development of accurate determination methods for trace OPEs in water is urgent for understanding the fate and risk of this class of emerging pollutants. However, the wide use of OPEs in experimental materials results in blank interference, which influences the accuracy of analytical results. In the present work, blank contamination and recovery of pretreatment procedures for analysis of OPEs in water samples were systematically examined for the first time. Blank contaminations were observed in filtration membranes, glass bottles, solid phase extraction cartridges, and nitrogen blowing instruments. These contaminations could be as high as 6.4–64 ng/L per treatment. Different kinds of membranes were compared in terms of contamination levels left after common glassware cleaning, and a special wash procedure was proposed to eliminate the contamination from membranes. Meanwhile, adsorption of highly hydrophobic OPEs on the inside wall of glass bottles was found to be 42.4%–86.1%, which was the primary cause of low recoveries and was significantly reduced by an additional washing step with acetonitrile. This work is expected to provide guidelines for the establishment of analysis methods for OPEs in aqueous samples.展开更多
During the last few years,various flow-based separation/preconcentration methodologies have gained pertinent novel advances and exhibited powerful capability in the field of sample pretreatment and their hyphenation w...During the last few years,various flow-based separation/preconcentration methodologies have gained pertinent novel advances and exhibited powerful capability in the field of sample pretreatment and their hyphenation with detection by atomic spectrometry.The present mini-review presents and discusses the progress of flow-based sample processing approaches commonly used for the assay of trace elemental species with detection by atomic spectrometry,including preliminary sample pretreatment,solid phase extraction(including solid phase microextraction),liquid-liquid extraction,vapor generation and dialysis techniques.Special emphasis has been paid on the novel applications and analytical procedures hyphenated with atomic spectrometry.The future perspectives of flow-based sample pretreatment protocols in the determination of trace elements and their speciation are also discussed.展开更多
基金This work was supported by the National Key Research and Development Program of China(2016YFD0500600)the Fund for Guangdong Enterprise Science and Technology Commissioner(GDKTP2020032200).
文摘Porcine epidemic diarrhea virus(PEDV),as the main causative pathogen of viral diarrhea in pigs,has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to the swine industry.Rapid diagnosis methods are essential for preventing outbreaks and transmission of this disease.In this study,a paper-based lateral flow immunoassay for the rapid diagnosis of PEDV in swine fecal samples was developed using stable color-rich latex beads as the label.Under optimal conditions,the newly developed latex bead-based lateral flow immunoassay(LBs-LFIA)attained a limit of detection(LOD)as low as 10^(3.60) TCID_(50)/mL and no cross-reactivity with other related swine viruses.To solve swine feces impurity interference,by adding a filtration unit design of LFIA without an additional pretreatment procedure,the LBs-LFIA gave good agreement(92.59%)with RT-PCR results in the analysis of clinical swine fecal samples{n=108),which was more accurate than previously reported colloidal gold LFIA(74.07%)and fluorescent LFIA(86.67%).Moreover,LBs-LFIA showed sufficient accuracy(coefficient of variance[CV]<15%)and stable(room temperature storage life>56 days)performance for PEDV detection,which is promising for on-site analysis and user-driven testing in pig production system.
基金financially supported by the National High Technology Research and Development Program of China (No.2013AA065201)Chinese Academy of Sciences (Nos.XDB14020101, YSW2013A01, YSW2013B01)
文摘Organophosphate esters（OPEs）, used as flame retardants and plasticizers, are widely present in environmental waters. Development of accurate determination methods for trace OPEs in water is urgent for understanding the fate and risk of this class of emerging pollutants. However, the wide use of OPEs in experimental materials results in blank interference, which influences the accuracy of analytical results. In the present work, blank contamination and recovery of pretreatment procedures for analysis of OPEs in water samples were systematically examined for the first time. Blank contaminations were observed in filtration membranes, glass bottles, solid phase extraction cartridges, and nitrogen blowing instruments. These contaminations could be as high as 6.4–64 ng/L per treatment. Different kinds of membranes were compared in terms of contamination levels left after common glassware cleaning, and a special wash procedure was proposed to eliminate the contamination from membranes. Meanwhile, adsorption of highly hydrophobic OPEs on the inside wall of glass bottles was found to be 42.4%–86.1%, which was the primary cause of low recoveries and was significantly reduced by an additional washing step with acetonitrile. This work is expected to provide guidelines for the establishment of analysis methods for OPEs in aqueous samples.
基金supported by the National Natural Science Foundation of China (21075013,21005011,21005013)the National Science & Technology Pillar Program (2012BAF14B09)the Fundamental Research Funds for the Central Universities (N110805001,N110705002,N100305003,N100305004)
文摘During the last few years,various flow-based separation/preconcentration methodologies have gained pertinent novel advances and exhibited powerful capability in the field of sample pretreatment and their hyphenation with detection by atomic spectrometry.The present mini-review presents and discusses the progress of flow-based sample processing approaches commonly used for the assay of trace elemental species with detection by atomic spectrometry,including preliminary sample pretreatment,solid phase extraction(including solid phase microextraction),liquid-liquid extraction,vapor generation and dialysis techniques.Special emphasis has been paid on the novel applications and analytical procedures hyphenated with atomic spectrometry.The future perspectives of flow-based sample pretreatment protocols in the determination of trace elements and their speciation are also discussed.
