Neuropathic pain is a severe and chronic condition widely found in the general population.The reason for this is the extensive variety of damage or diseases that can spark this unpleasant constant feeling in patients....Neuropathic pain is a severe and chronic condition widely found in the general population.The reason for this is the extensive variety of damage or diseases that can spark this unpleasant constant feeling in patients.During the processing of pain,the dorsal root ganglia constitute an important region where dorsal root ganglion neurons play a crucial role in the transmission and propagation of sensory electrical stimulation.Furthermore,the dorsal root ganglia have recently exhibited a regenerative capacity that should not be neglected in the understanding of the development and resolution of neuropathic pain and in the elucidation of innovative therapies.Here,we will review the complex interplay between cells(satellite glial cells and inflammatory cells)and factors(cytokines,neurotrophic factors and genetic factors)that takes place within the dorsal root ganglia and accounts for the generation of the aberrant excitation of primary sensory neurons occurring in neuropathic pain.More importantly,we will summarize an updated view of the current pharmacologic and nonpharmacologic therapies targeting the dorsal root ganglia for the treatment of neuropathic pain.展开更多
Maxillary first molar usually exhibits a radicular anatomy of three roots and three or four canals. However, different anatomic variations like extra number of roots and canals are possible. For a successful treatment...Maxillary first molar usually exhibits a radicular anatomy of three roots and three or four canals. However, different anatomic variations like extra number of roots and canals are possible. For a successful treatment, clinicians must have well equipment and a thorough knowledge of the external and internal anatomy of teeth and its variation. Using of CBCT may help to locate extra canals by giving a chance to clinicians to see the root canal anatomy in 3-D view. The aim of this study was to present a case report about a diagnosis and treatment in maxillary first molar with three roots and five canals. It is concluded that the diagnosing and the treatment of unusual cases are key factors for successful endodontic treatment of these teeth.展开更多
Objective The aim of this study was to examine the effects of photodynamic therapy(PDT)on the expression of Nav1.7 in spinal dorsal root ganglion(DRG)neurons.Methods The primary DRG neurons from newborn SD rats were c...Objective The aim of this study was to examine the effects of photodynamic therapy(PDT)on the expression of Nav1.7 in spinal dorsal root ganglion(DRG)neurons.Methods The primary DRG neurons from newborn SD rats were cultured.The cells were identified by neuron-specific enolase immunofluorescence staining.DRG neurons were divided into four groups:control group,photosensitizer group,laser group,and PDT group.The cell viability was detected by a cell counting kit-8(CCK8)assay.qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of Nav1.7 in DRG neurons.Results The purity of the cultured primary DRG neurons was greater than 90%.Compared with the control group,no significant change was found in the cell viability of the photosensitizer group,while the viability in the laser group and the PDT group was significantly reduced.The mRNA and protein expression levels of Nav1.7 were significantly greater in the laser group and the PDT group than in the control group.At the same time,the mRNA and protein expression levels of Nav1.7 were greater in the laser group than in the PDT group.Conclusion Both laser and PDT could upregulate the expression of Nav1.7 in DRG neurons,and the promoting effect might be related to the pain induced by clinical treatment.This study provides a research basis for the use of laser and PDT to treat pain.A better understanding of the relationship between Nav1.7 and PDT can help clinicians better manage PDT-related pain.展开更多
Objective: To explore the clinical efficacy of neurogrowth factor in the treatment of gingival pain and swelling in patients with dental pulp necrosis after root canal therapy and the effect on the serum inflammatory ...Objective: To explore the clinical efficacy of neurogrowth factor in the treatment of gingival pain and swelling in patients with dental pulp necrosis after root canal therapy and the effect on the serum inflammatory cytokines. Methods: A total of 156 patients with gingival pain and swelling after root canal therapy due to dental pulp necrosis were included in the study and randomized into the control group (n=78) and teh treatment group (n=78). The patients in the control group were given metronidazole tablets. On the above basis, the patients in the treatment group were given local injection of neurogrowth factors. 10-day treatment was regarded as one course, and the patients were continuously treated for 2 courses. The improvement of clinical symptoms before and after treatment in the two groups was evaluated. Gingival sulcus index and serum inflammatory cytokines before and after treatment in the two groups were detected and compared. Results: When compared with before treatment, the periodontal soft tissue swelling, tooth mobility, and periapical pain scores after treatment in the two groups were significantly reduced, and those in the treatment group were significantly lower than those in the control group. When compared with before treatment, the gingival sulcus bleeding index 1 and 2 courses after treatment in the two groups was significantly reduced, and that 2 courses after treatment was significantly lower than that after 1 course treatment. The gingival sulcus bleeding index 1 and 2 courses after treatment in the treatment group was significantly lower than that in the control group. When compared with before treatment, the serum IL-8 and IL-6 levels after treatment in the two groups, and TNF-α level after treatment in the treatment group were significantly reduced, and the above indicators in the treatment group were significantly lower than those in the control group. Conclusions: The neurogrowth factors in the treatment of gingival pain and swelling in patients with dental pulp necrosis after root canal therapy can effectively improve the clinical symptoms, and inhibit the inflammatory reaction, with a significant efficacy.展开更多
基金under a contract of the“Nicolás Monardes”program(RC-0002-2021)from the Andalusian Health Service,Andalusian Regional Ministry of Health and Consumptionfunds from the Excellent Project from Andalusian Government(Proy Excel_00996)+8 种基金funded by the French Multiple Sclerosis Foundation(ARSEP,1259&1254)the National Multiple Sclerosis Society(NMSS,RG 5088-A-1)the program“Investissements d’Avenir”(ANR-10-IAIHU-06 and ANR-11-INBS-0011–Neur ATRIS)under a“Miguel Servet”contract(CP20-0049)from the Health Institute CarlosⅢ,Ministry of Science and Innovation,Spainreceived grants from Andalusian Government and the European Commission under the Seventh Framework Program of the European Union(agreement Num.291730,contract TAHUB-II-107)ARSEP 1254IBRO Return Home FellowshipAES2022 from Health Institute CarlosⅢ(PI22/01141)the Excellent Project from Andalusian Regional Ministry of University,Research and Innovation(Proy Excel_00996)。
文摘Neuropathic pain is a severe and chronic condition widely found in the general population.The reason for this is the extensive variety of damage or diseases that can spark this unpleasant constant feeling in patients.During the processing of pain,the dorsal root ganglia constitute an important region where dorsal root ganglion neurons play a crucial role in the transmission and propagation of sensory electrical stimulation.Furthermore,the dorsal root ganglia have recently exhibited a regenerative capacity that should not be neglected in the understanding of the development and resolution of neuropathic pain and in the elucidation of innovative therapies.Here,we will review the complex interplay between cells(satellite glial cells and inflammatory cells)and factors(cytokines,neurotrophic factors and genetic factors)that takes place within the dorsal root ganglia and accounts for the generation of the aberrant excitation of primary sensory neurons occurring in neuropathic pain.More importantly,we will summarize an updated view of the current pharmacologic and nonpharmacologic therapies targeting the dorsal root ganglia for the treatment of neuropathic pain.
文摘Maxillary first molar usually exhibits a radicular anatomy of three roots and three or four canals. However, different anatomic variations like extra number of roots and canals are possible. For a successful treatment, clinicians must have well equipment and a thorough knowledge of the external and internal anatomy of teeth and its variation. Using of CBCT may help to locate extra canals by giving a chance to clinicians to see the root canal anatomy in 3-D view. The aim of this study was to present a case report about a diagnosis and treatment in maxillary first molar with three roots and five canals. It is concluded that the diagnosing and the treatment of unusual cases are key factors for successful endodontic treatment of these teeth.
基金supported by Natural Science Foundation of Beijing Municipality(No.7202200)Central Military Commission Health Care Project(No.20BJZ13)Air Force Equipment Scientific Research Project(No.KJ20201A050226).
文摘Objective The aim of this study was to examine the effects of photodynamic therapy(PDT)on the expression of Nav1.7 in spinal dorsal root ganglion(DRG)neurons.Methods The primary DRG neurons from newborn SD rats were cultured.The cells were identified by neuron-specific enolase immunofluorescence staining.DRG neurons were divided into four groups:control group,photosensitizer group,laser group,and PDT group.The cell viability was detected by a cell counting kit-8(CCK8)assay.qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of Nav1.7 in DRG neurons.Results The purity of the cultured primary DRG neurons was greater than 90%.Compared with the control group,no significant change was found in the cell viability of the photosensitizer group,while the viability in the laser group and the PDT group was significantly reduced.The mRNA and protein expression levels of Nav1.7 were significantly greater in the laser group and the PDT group than in the control group.At the same time,the mRNA and protein expression levels of Nav1.7 were greater in the laser group than in the PDT group.Conclusion Both laser and PDT could upregulate the expression of Nav1.7 in DRG neurons,and the promoting effect might be related to the pain induced by clinical treatment.This study provides a research basis for the use of laser and PDT to treat pain.A better understanding of the relationship between Nav1.7 and PDT can help clinicians better manage PDT-related pain.
文摘Objective: To explore the clinical efficacy of neurogrowth factor in the treatment of gingival pain and swelling in patients with dental pulp necrosis after root canal therapy and the effect on the serum inflammatory cytokines. Methods: A total of 156 patients with gingival pain and swelling after root canal therapy due to dental pulp necrosis were included in the study and randomized into the control group (n=78) and teh treatment group (n=78). The patients in the control group were given metronidazole tablets. On the above basis, the patients in the treatment group were given local injection of neurogrowth factors. 10-day treatment was regarded as one course, and the patients were continuously treated for 2 courses. The improvement of clinical symptoms before and after treatment in the two groups was evaluated. Gingival sulcus index and serum inflammatory cytokines before and after treatment in the two groups were detected and compared. Results: When compared with before treatment, the periodontal soft tissue swelling, tooth mobility, and periapical pain scores after treatment in the two groups were significantly reduced, and those in the treatment group were significantly lower than those in the control group. When compared with before treatment, the gingival sulcus bleeding index 1 and 2 courses after treatment in the two groups was significantly reduced, and that 2 courses after treatment was significantly lower than that after 1 course treatment. The gingival sulcus bleeding index 1 and 2 courses after treatment in the treatment group was significantly lower than that in the control group. When compared with before treatment, the serum IL-8 and IL-6 levels after treatment in the two groups, and TNF-α level after treatment in the treatment group were significantly reduced, and the above indicators in the treatment group were significantly lower than those in the control group. Conclusions: The neurogrowth factors in the treatment of gingival pain and swelling in patients with dental pulp necrosis after root canal therapy can effectively improve the clinical symptoms, and inhibit the inflammatory reaction, with a significant efficacy.
