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First Babies from Cryopreserved Oocytes in Hungary
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作者 Konc J Kanyo K +2 位作者 Varga E Kriston R Cseh S 《Journal of Reproduction and Contraception》 CAS 2006年第1期47-50,共4页
Objective To evaluate the value of oocyte cryopreservation (CP) in our clinical ICSI program. Methods Freezing procedure with medium containing 1.5 mol/L propanediol (PrOH) + 0. 3 mol/L succrose and traditional ... Objective To evaluate the value of oocyte cryopreservation (CP) in our clinical ICSI program. Methods Freezing procedure with medium containing 1.5 mol/L propanediol (PrOH) + 0. 3 mol/L succrose and traditional slow-freezingprotocol were used. Thawed oocytes were fertilized with ICSI (4-6 h after thawing), and fertilization was assessed 12-16 h later. Laser assisted hatching was performed on all transferred embryos and embryo transfer was carried out 48-72 h after ICSI. Results Eighty-five eggs were thawed and survival rate of 75.3% (64/85) was obtained. Sixty-four oocytes were inseminated with ICSI, 47fertilized (47/64; 73.4%) and a cleavage rate of 85% (40/47) was obtained. Embryo transfers were performed in 18 patients, and 4 (19%) resulted in clinical pregnancies. One of the pregnancies encountered first trimester abortion. Implantation rate were 17.2% (5/29) per embryo and 5.8% (5/85) per egg thawed. In all cases, chorion biopsy was performed resulting 46 XY kariotype. Conclusion Our results provide further evidence of that although egg freezing cannot currently claim to be a routine procedure in human IVF,, there will certainly be a place for oocyte CP in reproductive medicine in the future. 展开更多
关键词 slow freezing oocyte cryopreservation cryoprotective additives propanechol SUCROSE oocyte survival
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