Ablotlc stresses, such as drought, high salinity, and cold/freezing, lead plants to produce excess reactive oxygen species. Catalase, a unique hydrogen peroxide-scavenging enzyme, plays a very Important role In plants...Ablotlc stresses, such as drought, high salinity, and cold/freezing, lead plants to produce excess reactive oxygen species. Catalase, a unique hydrogen peroxide-scavenging enzyme, plays a very Important role In plants. To characterize the catalase involved In plant response to ablotlc stresses, we constructed a cDNA library from 4℃-treated Festuca arundinacea Schreb seedlings and isolated a catalase gene from this library. The cDNA （FaCat1, 1 735 bp） contained an open reading frame of 1 479 bp. BLAST analysis Indicated that the deduced amino acid sequence showed 96% Identity with that from wheat TaCat1 and 87% Identity with that from maize ZmCat2. Northern blotting analysis showed an obvious Increase of FaCat1 transcripts In leaves In contrast with roots. Time-course analysis of the expression of FaCat1 in F. arundinacea leaves showed that FaCat1 expression was upregulated in cold- and salt-stressed leaves, with the FaCat1 transcripts accumulat-Ing mostly at 4 or 2 h after cold or salt stress, respectively. No significant changes in FaCat1 transcription were observed in dried leaves and inhibition of FaCat1 transcription was found In absclsic acid （ABA）-treated leaves, Indicating that the FaCat1 gene is differentially expressed during cold, high salt, drought, and ABA treatment In F. arundinacea leaves.展开更多
文摘Ablotlc stresses, such as drought, high salinity, and cold/freezing, lead plants to produce excess reactive oxygen species. Catalase, a unique hydrogen peroxide-scavenging enzyme, plays a very Important role In plants. To characterize the catalase involved In plant response to ablotlc stresses, we constructed a cDNA library from 4℃-treated Festuca arundinacea Schreb seedlings and isolated a catalase gene from this library. The cDNA （FaCat1, 1 735 bp） contained an open reading frame of 1 479 bp. BLAST analysis Indicated that the deduced amino acid sequence showed 96% Identity with that from wheat TaCat1 and 87% Identity with that from maize ZmCat2. Northern blotting analysis showed an obvious Increase of FaCat1 transcripts In leaves In contrast with roots. Time-course analysis of the expression of FaCat1 in F. arundinacea leaves showed that FaCat1 expression was upregulated in cold- and salt-stressed leaves, with the FaCat1 transcripts accumulat-Ing mostly at 4 or 2 h after cold or salt stress, respectively. No significant changes in FaCat1 transcription were observed in dried leaves and inhibition of FaCat1 transcription was found In absclsic acid （ABA）-treated leaves, Indicating that the FaCat1 gene is differentially expressed during cold, high salt, drought, and ABA treatment In F. arundinacea leaves.
