Improvement of the Optimum Temperature of Penicillium expansum Lipase by Site-Directed Mutagenesis

In order to improve the optimum temperature of lipases,the Penicillum expansum lipase(PEL)gene was mutated by site-directed mutagenesis using overlap extension PCR technique.The recombinant plasmid containing mutant E83V pPIC3.5K-lip-E83V was expressed in Pichia pastoris GS115.Comparison experiments of the mutant PEL-E83V-GS and the wild-type PEL-GS showed that the optimum temperature(45℃)of the mutant was 5℃ higher than that of the wild type.The thermostability of the mutant was similar to that of the wild type.The enzymatic activity of the mutant was 188 U/ml at 37℃,which was 80%that of the wild type in the same conditions.Hydrophobic interaction may be enhanced in the surface region by the hydrophilic amino acid Glu substituted with the hydrophobic amino acid Val,and may be responsible for the improvement of the optimum temperature.

Screening of Low Temperature Resistance Germplasm Resources of Pigment Pepper

Objective The aim of this study was to explore the method and standard for rapidly screening low temperature-resistant pepper germplasm resources and provide a theoretical basis for the breeding of low temperature-resistant pepper. [ Method ] With 110 pigment pepper seeds as the materials, their germination vigor under optimum temperature and suboptimal temperature were determined by means of roll rapid germination, and seeds with different genetic types were evaluated from aspects of germination vigor and its interval division. [ Result ] 37 pepper seeds with stronger low temperature resistance were screened. [ Conclusion]This study provides an important basis for screening low temperature-resistant pepper germplasm resources.

钒酸钇晶体(YVO_4、Nd∶YVO_4)生长与退火温场的优化设计

Once the growth method for a given crystal has been decided,one should be able to adjust a number of growth parameters in order to achieve best results.These parameters include ambient growth atmosphere,temperature gradient of growth furnace,percentage of fill of crucible,rotation and pulling rates.Among them,the most delicate thing we face is to design and create suitable temperature gradient for a given material according to its thermal properties and growth behavior.For YVO 4 crystals,this is particularly important because of its poor conductivity and strong thermo mechanical anisotropy.Optimum temperature gradient for the growth of large YVO 4 crystals should be as large as possible during the growth process,while be as small as possible during the annealing stage to alleviate thermal stress and avoid cleavage.

Yield and mechanical properties of veneer from Brachystegia nigerica

In an effort to find suitable wood from natural forest to meet the demand for veneer products, the yield and tensile strength of veneers produced from Brachystegia nigerica were investigated. Two trees of B. nigerica were separately selected from 10 different natural forest zones while two logs were obtained from each tree. The logs were debarked and steamed in a vat prior to rotary peeling and slicing for veneer production. The optimum steam temperature was determined by considering different temperatures： 50℃, 60℃, 70℃, 80℃ and 90℃ for 24 h. Thereafter, optimum steam time was determined at the optimum temperature by considering durations of 24, 48, 72 and 96 h. The average taper of 0.75 mm per 1.0 m length was recorded for B. nigerica, indicating that the logs were reasonably cylindrical; thereby its logs are good for the production of veneer. The yield ranged from 44% to 61% with an average of 52% of the log input. The tensile strength of the veneer was tested perpendicular to grain and both peeled and sliced veneers had the highest tensile strength between 70℃ and 90℃, suggesting that softening of wood polymers, especially lignin, is between 70℃ and 90℃. The optimum temperature and time for veneer production are 70℃ and 48 h, respectively. Commercial production of veneer from B. nigerica is feasible based on the yield and mechanical properties of the obtained veneer, thereby encouraging the expansion of the scope of its utilization.

Exergy Analysis of Photo-Thermal Interaction Process between Solar Radiation Energy and Solar Receiver

A unified theory of non-equilibrium radiation thermodynamics is always in search as it is meaningful for solar energy utilization.An exergy analysis of photo-thermal interaction process between the solar radiation energy and solar receiver is conducted in this paper.The non-equilibrium radiation thermodynamic system is described.The thermodynamic process of photo-thermal interaction between the solar radiation and solar receiver is introduced.Energy,exergy and entropy equations for the photo-thermal process are provided.Formulas for calculating the optimum receiving temperatures of the solar receiver under both non-concentration and solar concentration conditions are presented.A simple solar receiver is chosen as the calculation example to launch the exergy analysis under non-concentration condition.Furthermore,the effect analysis of solar concentration on the thermodynamic performance of the solar receiver for solar thermal utilization is carried out.The analysis results demonstrate that both the output exergy flux and efficiency of the solar receiver can be improved by increasing the solar concentration ratio during the solar thermal utilization process.The formulas and results provided in this paper may be used as a theoretical reference for the further studies of non-equilibrium radiation thermodynamic theory and solar thermal utilization.