Apples to oranges:environmentally derived,dynamic regulation of serotonin neuron subpopulations in adulthood?

Traumatic brain injury(TBI)is a public health problem with an undue economic burden that impacts nearly every age,ethnic,and gender group across the globe(Capizzi et al.,2020).TBIs are often sustained during a dynamic range of exposures to energetic environmental forces and as such outcomes are typically heterogeneous regarding severity and pathology(Capizzi et al.,2020).

Preliminary Studies on CPG/Hinf Ⅰ RFLP Groups of Citrus tristeza virus Infected Sweet Oranges in China

Citrus tristeza virus （CTV） causes economically important losses to the citrus industry worldwide. Mild strain cross protection （MSCP） against tristeza has hardly been practised due to mixed infection of different CTV-strains and little background of its molecular biology in China. For better cognition on CTV, 192 sweet orange samples collected from eight provinces （Chongqing, Sichuan, Fujian, Hunan, Guangxi, Yunnan, Guangdong and Jiangxi） were tested by direct tissue blot immuno-assay （DTBIA）, and 158 of them were tested positively, which therefore were subjected to coat protein gene （CPG）/Hinf Ⅰ restriction fragment length polymorphism （RFLP） analysis. Sample bulks were compared between Chongqing and Fujian by some statistical data, including ratios of single infection and mixed infection to local samples, proportions of CTV isolates with single RFLP groups, and rates of each RFLP group. The simplified analysis of samples from the other six provinces were then conducted. This study suggests that CTV isolates with CPG/Hinf Ⅰ RFLP groups Ⅲ and Ⅰ are the main epidemic ones in China, and mixed infection of CTV in fields are popular. Based on observation of severity of stem-pitting symptom in field trees, CTV isolates with CPG/Hinf Ⅰ RFLP groups Ⅲ and Ⅰ caused severe stem-pittings in sweet oranges in China.

Isolation and Purification of Anthocyanins from Blood Oranges by Column Chromatography

In this study, isolation and purification of anthocyanins from blood oranges by column chromatography were investigated, and then the anthocyanins of blood orange were identified. The behaviors of static adsorption and desorption, dynamic adsorption and desorption of 12 kinds of resins were compared. The results indicated that NKA-9 macroporous resin was optimum for isolation of blood orange anthocyanins, and the optimal elution reagent was 50% ethanol with citric acid （pH 2.5）. Toyopearl TSK HW-40S column was employed to separate and purify the anthocyanin extracts from blood orange. The best separation of Toyopearl TSK HW-40S column was obtained using a mobile phase of 35% methanol with 2% formic acid at a flow-rate of 0.6 mL min-~. Three kinds of anthocyanins were purified from blood orange. Then, the anthocyanins of blood orange were identified by HPLC-ESUMS analysis. The results showed that cyanidin-3-glucoside （35.2%） and cyaniding-3-（6＂-malonyl） glucoside （42.9%） were the major anthocyanins of blood orange. Furthermore, cyanidin-3-（3＂-malonyl） glucoside, cyanidin 3-（6＂-dioxalyl） glucoside and cyanidin-3-glucoside adduct：4-vinylcatechol were identified in blood orange. The combination of NKA-9 macroporous resin and Toyopearl TSK HW-40S column chromatography for isolation and purification of blood orange anthocyanins was an effective method, and HPLC-ESI/MS analysis was a convenient, rapid and effective method for identification of anthocyanins from blood orange.

Effect of Sustained Deficit Irrigation on Stem Water Potential of Navel Oranges in Jordan Valley

Research was conducted to find the relationship between deficit irrigation treatments （DIT） and stems water potential. The study was conducted on 14 years old navel orange trees grafted on sour oranges for the growing season 2006/2007 at a private farm in the Northern part of Jordan Valley （latitude： 32° 50′ N, longitude： 32° 50′ E, altitude： -254 m）. Three levels of irrigation treatments （IT） were applied; namely 100%, 75% and 50% of reference evapotranspiration, representing over irrigation （OIT）, full irrigation （FIT）, and deficit irrigation （DIT）, respectively. A drip irrigation using one irrigation source line with drippers spaced 0.5 m having average discharge of 2.3 L/hr at pressure 1.5 bar, was used. Stem water potential （SWP） at 100% over irrigation treatment （OIT） of navel orange trees had less negative value during the irrigation seasons （-1.57 MPa）, whereas the highest negative value （-2.17 MPa） occurred at 50% deficit irrigation treatment （DIT）.

Changes in phenolic content,composition,and antioxidant activity of blood oranges during cold and on-tree storage

Citrus fruits are rich in phenolic compounds that possess several health benefits.However,few studies have focused on the changes in phenolic compounds in citrus fruits during postharvest storage.This study dynamically monitored the phenolic content,components and antioxidant activity of‘Tarocco’blood oranges during a period of 12-week cold storage and on-tree storage,respectively.We investigated the alteration mechanism of phenolic compounds in blood oranges by evaluating phenylpropanoid pathway-related enzyme activities and gene expression.Results showed that flavanones were the main phenolic compounds in blood oranges.Both storage methods mainly stimulated the accumulation of phenolic acids to improve total phenolic content,which reached the maximum at week 12.Nonetheless,blood oranges had a higher phenolic content and antioxidant activity under on-tree storage than cold storage.Furthermore,the enzyme activities and gene expression of the phenylpropanoid pathway demonstrated that the accumulation of phenolics in blood oranges during storage was highly related to the activation of the phenylpropanoid pathway.These results demonstrate that on-tree storage is a potential approach for extending the supply period of blood orange from the perspective of phenolic compounds.

In Vitro Development and Germination of Immature Embryos of Sweet Oranges Parson Brown and Pineapple(Citrus sinensis)

Oranges are classified as the most traded fruit variety worldwide.The diversification of the varietal profile of this group is among the objectives of the Citrus breeding program.So,the creation of new triploid hybrids via immature embryos rescue,can lead to an increase in gene pool and produce seedless varieties.In addition,flow cytometry was successfully used for analyzing ploidy levels and genetic origin of triploid plants.The present study was aimed at optimizing immature embryos in vitro germination and plantlet development based on medium chemical composition of the two orange varieties,Pineapple and Parson Brown.When the fruit gets matured,they are harvested and the seeds extracted are classified according to their size.Only those which are small are cultured in a sterile Murashige and Tucker(MT)basal medium.The different concentrations of growth regulators are put to the test in order to gain the best medium for seeds development.M1(MT+1 mg/L gibberellic acid(GA3)),M2(MT+1 mg/L kenitin+0.5 mg/L 6-benzylaminopurine(BAP)+0.1 naphthalene acetic acid(NAA)),M3(MT+25 mg/L adenine sulfate(ADS)),M4(MT+0.5 mg/L kenitin+0.5 mg/L BAP+1 mg/L GA3).Percentage of germinated embryos(PGE)is the highest in M3 with 100%and 90%for both Pineapple and Parson Brown varieties,respectively.In addition,the germination interval average(GIA)is shorter in M1 for both varieties,which takes 6 d and 7 d,respectively.As regard to speed seedling growth(mm/week),both varieties undergo significant change in all four mediums.Similarly,the highest rate of acclimatization for the Pineapple and Parson Brown varieties is 80%and 90%in M1.In general,the smaller the embryos are,the more sensitive they are to culture medium composition.It is therefore essential to optimize the medium components in order to stimulate their in vitro development.Therefore,M1(MT+1 mg/L GA3)remains the best to promote short term good germination and produce a better rate of acclimatization.

Fast detection of the early decay in oranges using visible-LED structuredillumination imaging combined with spiral phase transform and feature-based classification model

The early decay of citrus can cause economic and serious food safety issues.The early decayed area has no obvious visual characteristics,making effective detection of this damage very difficult for the citrus industry.This study constructed a new detection system based on visible-light emitting diode(LED)structured-illumination imaging and proposed an effective methodology combined with a spiral phase transform(SPT)algorithm for the early detection of decayed oranges.Each sample obtained three phase-shifting pattern images with phase shifts of−2π/3,0,and 2π/3 at a spatial frequency of 0.25 cycles/mm.Three strategies(i.e.,the conventional three-phase-shifting method,2-phase SPT,and 1-phase SPT)were used to demodulate the original patterned images to recover the direct component(DC)and amplitude component(AC)images.The partial least squares discriminant analysis(PLS-DA)and least squares support vector machine(LS-SVM)classification models were established based on the texture features of DC,AC,and RT(i.e.the ratio of AC to DC)images.Then,the random frog(RF)algorithm was used to simplify the optimal full-featured model.Finally,the LS-SVM model constructed using 7 texture features from the RT image obtained an average classification accuracy of 95.1%for all tested samples.This study indicates that the proposed structured-illumination imaging technique combined with 2-phase SPT and feature-based classification model can achieve the fast identification of early decayed oranges.

烟草Orange蛋白突变体的转录组和代谢组分析

【目的】分析烟草Orange蛋白突变体的转录组和代谢组,为培育高类胡萝卜素含量的烟草提供理论依据。【方法】通过CRISPR/Cas9基因编辑技术,获得带有3个碱基缺失的NtOR基因突变株系208。采用转录组测序、液相色谱—质谱联用的非靶向方法、气相色谱—质谱联用的衍生化方法和顶空进样技术,对突变株系的差异基因和差异代谢物进行分析。【结果】突变株系208叶片中的β-胡萝卜素和紫黄质含量显著下降。转录组分析显示:突变株系208中有10369个差异表达基因,其中4178个基因上调,6191个基因下调。代谢组分析显示:突变株系208与对照相比有160个差异代谢物。【结论】突变株系208中的类胡萝卜素含量下降,色素代谢途径中玉米黄素环氧化酶基因下调,有机酸和二萜类代谢物含量显著升高。

High-Performance Organic Field-Effect Transistors Based on Two-Dimensional Vat Orange 3 Crystals

The exploration and research of low-cost,environmentally friendly,and sustainable organic semiconductor materials are of immense significance in various fields,including electronics,optoelectronics,and energy conversion.Unfortunately,these semiconductors have almost poor charge transport properties,which range from∼10^(−4) cm^(2)·V^(−1)·s^(−1) to∼10^(−2) cm^(2)·V^(−1)·s^(−1).Vat orange 3,as one of these organic semiconductors,has great potential due to its highly conjugated structure.We obtain high-quality multilayered Vat orange 3 crystals with two-dimensional(2D)growth on h-BN surfaces with thickness of 10–100 nm using physical vapor transport.Raman’s results confirm the stability of the chemical structure of Vat orange 3 during growth.Furthermore,by leveraging the structural advantages of 2D materials,an organic field-effect transistor with a 2D vdW vertical heterostructure is further realized with h-BN encapsulation and multilayered graphene contact electrodes,resulting in an excellent transistor performance with On/Off ratio of 104 and high field-effect mobility of 0.14 cm^(2)·V^(−1)·s^(−1).Our results show the great potential of Vat orange 3 with 2D structures in future nano-electronic applications.Furthermore,we showcase an approach that integrates organic semiconductors with 2D materials,aiming to offer new insights into the study of organic semiconductors.

Global analysis of basic leucine zipper transcription factors in trifoliate orange and the function identification of PtbZIP49 in salt tolerance

As one of the most widely distributed and highly conserved transcription factors in eukaryotes,basic leucine zipper proteins(bZIPs)are involved in a variety of biological processes in plants,but they are largely unknown in citrus.In this study,56 bZIP family members were identified genome-wide from an important citrus rootstock,namely trifoliate orange(Poncirus trifoliata L.Raf.),and these putative bZIPs were named PtbZIP1—PtbZIP56.All PtbZIPs were classified into 13 subgroups by phylogenetic comparison with Arabidopsis thaliana bZIPs(AtbZIPs),and they were randomly distributed on nine known(50 genes)chromosomes and one unknown(6 genes)chromosome.Sequence analysis revealed the detailed characteristics of PtPZIPs,including their amino acid length,isoelectric point(pI),molecular weight(MW),predicted subcellular localization,gene structure,and conserved motifs.Prediction of promoter elements suggested the presence of drought,low-temperature,wound,and defense and stress responsive elements,as well as multiple hormone-responsive cis-acting elements.Spatiotemporal expression analysis showed the transcriptional patterns of PtbZIPs in different tissues and under dehydration,high salt,ABA,and IAA treatments.In addition,21 PtbZIPs were predicted to have direct or indirect protein—protein interactions.Among these,PtbZIP49 was experimentally proven to interact with PtbZIP1 or PtbZIP11 by using a yeast two-hybrid assay and bimolecular fluorescence complementation(BiFC).Subcellular localization analysis further revealed that PtbZIP1,PtbZIP11,and PtbZIP49 were localized in the nucleus.Moreover,PtbZIP49 was functionally identified as having an important role in salt stress via ectopic expression in A.thaliana and silenced in trifoliate orange using virus-induced gene silencing(VIGS).This study provided comprehensive information on PtbZIP transcription factors in citrus and highlights their potential functions in abiotic stress.

Green Synthesis of Silver Nanoparticles Using Aqueous Orange and Lemon Peel Extract and Evaluation of Their Antimicrobial Properties

Green synthesis of silver nanoparticles (AgNPs) using aqueous extracts of orange and lemon peels, as a reducing agent, and silver nitrate salts as a source of silver ions is a promising field of research due to the versatility of biomedical applications of metal nanoparticles. In this paper, AgNPs were synthetized at different reaction parameters such as the type and concentration of the extracts, metal salt concentration, temperature, speed stirring, and pH. The antibacterial properties of the obtained silver nanoparticles against E. coli, as well as the physical and chemical characteristics of the synthesized silver nanoparticles, were investigated. UV-Vis spectroscopy was used to confirm the formation of AgNPs. In addition to green biogenic synthesis, chemical synthesis of silver nanoparticles was also carried out. The optimal temperature for extraction was 65˚C, while for the synthesis of AgNPs was 35˚C. The synthesis is carried out in an acidic environment (pH = 4.7 orange and pH = 3.8 lemon), neutral (pH = 7) and alkaline (pH = 10), then for different concentrations of silver nitrate solution (0.5 mM - 1 mM), optimal time duration of the reaction was 60 min and optimal stirring speed rotation was 250 rpm on the magnetic stirrer. The physical properties of the synthesized silver nanoparticles (conductivity, density and refractive index) were also studied, and the passage of laser light through the obtained solution and distilled water was compared. Positive inhibitory effect on the growth of new Escherichia coli colonies have shown AgNPs synthesized at a basic pH value and at a 0.1 mM AgNO3 using orange or lemon peel extract, while for a 0.5 mM AgNO3 using lemon peel extract.

Design of an Interconnection Architecture and Sizing of Two (2) EPC Core Networks: The Case of Orange-Guinea

With the arrival of the 4G and 5G,the telecommunications networks have experienced a large expansion of these networks.That enabled the integration of many services and adequate flow,thus enabling the operators to respond to the growing demand of users.This rapid evolution has given the operators to adapt,their methods to the new technologies that increase.This complexity becomes more important,when these networks include several technologies to access different from the heterogeneous network like in the 4G network.The dimensional new challenges tell the application and the considerable increase in demand for services and the compatibility with existing networks,the management of mobility intercellular of users and it offers a better quality of services.Thus,the proposed solution to meet these new requirements is the sizing of the EPC(Evolved Packet Core)core network to support the 5G access network.For the case of Orange Guinea,this involves setting up an architecture for interconnecting the core networks of Sonfonia and Camayenne.The objectives of our work are of two orders:(1)to propose these solutions and recommendations for the heart network EPC sizing and the deployment to be adopted;(2)supply and architectural interconnection in the heart network EPC and an existing heart network.In our work,the model of traffic in communication that we use to calculate the traffic generated with each technology has link in the network of the heart.

An Analysis of Specific Categories of Birth Defects and Developmental Disabilities for Children of Participants of the Air Force Health Study

Background: The Air Force Health Study collected reproductive outcomes for live-born children of male Air Force veterans of the Vietnam War. Methods: Dioxin values for participants were obtained from blood samples. Analyses were conducted of occurrence of 16 specific categories of birth defects and developmental disabilities. Children were categorized as conceived before and after the start of participants’ Vietnam War service. Children conceived before the start of Vietnam War service were treated as being conceived when their fathers had unquantifiable dioxin values. Children conceived after the start of Vietnam War service for participants with missing dioxin values were excluded from primary analyses, but were used to assess the impact of their exclusion on conclusions. Correlation between values for specific categories for multiple children fathered by the same participant was accounted for. The dose-response relationship was treated as a step function increasing for dioxin values larger than adaptively identified individual thresholds changing with the specific category. Results: For 15 of 16 specific categories, the probability of occurrence increased substantially for a sufficiently high dioxin level above identified thresholds. Exclusion of children due to missing dioxin likely did not affect these results. Conclusions: Results supported the conclusion of substantial adverse effects on a wide variety of specific categories of birth defects and developmental disabilities due to sufficiently high exposures to dioxin, a toxic contaminant of Agent Orange used for herbicide spraying in the Vietnam War. Results may hold more generally, but might also have been affected by a variety of limitations.

PERSIMMONS

Persimmons are a delicious fruit that grows on trees.They can be round or long and can come in different colors,such as orange and red.Persimmons taste sweet and a bit like honey.People think of persimmons as a fall fruit because they are ripe in the fall.People can pick them and eat them fresh from the tree.They can also use them to make dried persimmons.

Do You Understand Addition?

I.Add to find how many cubes there are in all.(1)There is 1 purple cube and 2 yellow cubes.(2)There are 2 orange cubes and 3 blue cubes.(3)There are 5 green cubes and 1 purple cube.(4)There are 3 orange cubes and 1 blue cube.

Cranberry Sauce Recipe

Cranberry Sauce Ingredients 1 cupsugar 1/2 cup orange juice 1/2cup water 4cups fresh orfrozen cranberries 1 piece of orange peel Directions 1.Rinsethecranberries.2.Put sugar,orange juice and water in a pot.Let it boil.

橘子洲风情画卷

During Labour Day this year,Orange Isle was undoubtedly one of the most popular tourist destinations in Changsha.Located between Yuelu Mountain and Tianxin Pavilion,this thousand-year-old sandbar has long been a natural paradise from the past to the present.The name of Orange Isle may originate from the poem,in which the isle is described as“The orange grows by the side of the Xiang River”(橘生湘水侧).

PET FISH

Do you want some quiet,colorful pets?Try fish!Get a tank and add clean water to it.Now you have a home for fish!Goldfish are orange,red or yellow.Guppies are small and fun.They can be many colors.Fighting fish have wide and beautiful tails.You can also add little shrimp to a fish tank.Clean the tank for the fish.Put plants in your tank,too.Plants add airto the water.

Color of the Year 2024: Peach Fuzz

Pantone has declared Pantone 13-1023Peach Fuzz as its Color of the Year for 2024.This soft peach tone reflects comforting warmth and represents a desire for unity,peace and personal well-being.The calming shade,a sublime blend of pink and orange,suggests fresh softness,signifying an environment that invites relaxation,reccovery and growth.The delicatehue of Pantone 13-1023 Peach Fuzz not only awakens our senses to the tactile and enveloping warmth of the color,but also influences our sense of peace and overall well-being.

Review and Analysis: Evaluation of the Impacts and Consequences of Using Agricultural Herbicides as Military Chemical Weapons in Second Indochina War

The legacy of the human misery caused by the application of the herbicides including Agent Purple and Agent Orange contaminated with unknown amounts of dioxin TCDD and Agent Blue, the arsenic-based herbicide, sprayed over the jungles, rice fields, and hamlets of Vietnam is still haunting us today. Why did this happen? Could it have been prevented? Was it necessary United States military strategy? Was it an intentional decision to inflict this blight on the enemy soldiers and the Vietnamese, Cambodian, and Laotian civilians, to poison their land and cause generations of harm? Alternatively, was it an unpreventable accident in the march of military history? What patterns in the U.S. government’s thought process could be identified as the cause, which led to the decision to use these herbicides as tactical chemical weapons? If the introduction of herbicide (chemical) weapons had not been made, would the outcome of the Vietnam War and the Secret Wars in Laos and Cambodia have been any different? The objective of this treatise is to outline the role of world events and backgrounds and the role of the leaders, U.S. military, CIA, USDA, U.S. State Department, the U.S. President appointed Ambassadors to Vietnam and Laos, chemical companies, and President Diệm’s Republic of Vietnam (RVN) government and military. Their collective advice led to the decision to use herbicides as military and environmental chemical weapons in the Second Indochina War. Were the National interests achieved by U.S. military strategy in the RVN using herbicide weapons worth the long-term environmental and human health consequences in Vietnam, Cambodia, and Laos? Did it impact the outcome of the Second Indochina War?