To address the issues for assessing and prospecting the replaceable resource of crisis mines, a geological ore-controlling field model and a mineralization distribution field model were proposed from the viewpoint of ...To address the issues for assessing and prospecting the replaceable resource of crisis mines, a geological ore-controlling field model and a mineralization distribution field model were proposed from the viewpoint of field analysis. By dint of solving the field models through transferring the continuous models into the discrete ones, the relationship between the geological ore-controlling effect field and the mineralization distribution field was analyzed, and the quantitative and located parameters were extracted for describing the geological factors controlling mineralization enrichment. The method was applied to the 3-dimensional localization and quantitative prediction for concealed ore bodies in the depths and margins of the Daehang mine in Guangxi, China, and the 3-dimensional distribution models of mineralization indexes and ore-controlling factors such as magmatic rocks, strata, faults, lithology and folds were built. With the methods of statistical analysis and the non-linear programming, the quantitative index set of the geological ore-controlling factors was obtained. In addition, the stereoscopic located and quantitative prediction models were set up by exploring the relationship between the mineralization indexes and the geological ore-controlling factors. So far, some concealed ore bodies with the resource volume of a medium-sized mineral deposit are found in the deep parts of the Dachang Mine by means of the deep prospecting drills following the prediction results, from which the effectiveness of the predication models and results is proved.展开更多
Rare metals including Lithium(Li),Beryllium(Be),Rubidium(Rb),Cesium(Cs),Zirconium(Zr),Hafnium(Hf),Niobium(Nb),Tantalum(Ta),Tungsten(W)and Tin(Sn)are important critical mineral resources.In China,rare metal mineral dep...Rare metals including Lithium(Li),Beryllium(Be),Rubidium(Rb),Cesium(Cs),Zirconium(Zr),Hafnium(Hf),Niobium(Nb),Tantalum(Ta),Tungsten(W)and Tin(Sn)are important critical mineral resources.In China,rare metal mineral deposits are spatially distributed mainly in the Altay and Southern Great Xingán Range regions in the Central Asian orogenic belt;in the Middle Qilian,South Qinling and East Qinling mountains regions in the Qilian-Qinling-Dabie orogenic belt;in the Western Sichuan and Bailongshan-Dahongliutan regions in the Kunlun-Songpan-Garze orogenic belt,and in the Northeastern Jiangxi,Northwestern Jiangxi,and Southern Hunan regions in South China.Major ore-forming epochs include Indosinian(mostly 200-240 Ma,in particular in western China)and the Yanshanian(mostly 120-160 Ma,in particular in South China).In addition,Bayan Obo,Inner Mongolia,northeastern China,with a complex formation history,hosts the largest REE and Nb deposits in China.There are six major rare metal mineral deposit types in China:Highly fractionated granite;Pegmatite;Alkaline granite;Carbonatite and alkaline rock;Volcanic;and Hydrothermal types.Two further types,namely the Leptynite type and Breccia pipe type,have recently been discovered in China,and are represented by the Yushishan Nb-Ta-(Zr-Hf-REE)and the Weilasituo Li-Rb-Sn-W-Zn-Pb deposits.Several most important controlling factors for rare metal mineral deposits are discussed,including geochemical behaviors and sources of the rare metals,highly evolved magmatic fractionation,and structural controls such as the metamorphic core complex setting,with a revised conceptual model for the latter.展开更多
The appropriate content and distribution of sinter moisture play an important role in the granulation of iron ores. In this study, the effects of porosity, size distribution, and particle shape on the water absorption...The appropriate content and distribution of sinter moisture play an important role in the granulation of iron ores. In this study, the effects of porosity, size distribution, and particle shape on the water absorption rate (WAR) of four types of iron ores were analyzed by using the immersion method and capillary water absorption method. In addition, the mechanism underlying the water absorption process in iron ores was unraveled. It is found that the WARs of iron ores decrease quickly with the increase in water absorption time at the initial stages of water absorption. With further increase in absorption time, the WARs decrease gradually, until near 0. Iron ores with higher porosity, smaller particle size, and plate-like structure have the higher WARs. Compared with pores in the single-particle iron ore, voids among particles in the multi-particle iron oxide play an important role at the initial stages of water absorption. The water absorption mechanism of all single-particle and multi-particle iron ores analyzed in this study includes four steps, wherein the first three steps play a significant role in the sintering pro-cess.展开更多
Sinter body strength, which reflects the strength of sinter, plays an important role in the improvement of sinter. In this study, the sinter body strengths of iron ores were measured using a microsintering method. The...Sinter body strength, which reflects the strength of sinter, plays an important role in the improvement of sinter. In this study, the sinter body strengths of iron ores were measured using a microsintering method. The relationship between the chemical composition and sinter body strength was discussed. Moreover, sinter-pot tests were performed. The effects of sinter body strength on the sintering indexes were then elucidated, and the bottom limit of sinter body strength of blending ores was confirmed. In the results, the compressive strengths(CSs) of iron ores are observed to decrease with the increasing of the contents of loss on ignition(LOI), SiO 2, and Al2O3; however, LOI of less than 3wt% does not substantially influence the CSs of fine ores. In the case of similar mineral composition, the porosity, in particular, the ratio between the number of large pores and the total number of pores, strongly influences the sinter body strength. With an increase of the blending-ore CSs used in sinter-pot tests, the yield, productivity, and tumbler strength increase, and the solid fuel consumption decreases. The CSs of the blending ores only slightly affect the sintering time. The CS bottom limit of the blending ores is 310 N. When the CSs of the blending ores increase by 10%, the yield, productivity, and tumbler index increase by 1.9%, 2.8%, and 2.0%, respectively, and the solid fuel consumption decreases by 1.9%.展开更多
A detailed investigation on 3D spatial distribution rules of Banded Iron-bearing Formation(BIF) with methods of gravity-magnetic inversion and 3D modeling of iron mine is presented based on the former analysis in the ...A detailed investigation on 3D spatial distribution rules of Banded Iron-bearing Formation(BIF) with methods of gravity-magnetic inversion and 3D modeling of iron mine is presented based on the former analysis in the Anshan-Benxi area.Three dimension spatial distribution types of BIF are concluded as hook-like,tabularlike and "W"-like.BIF was mainly developed in three types of space including(1) the syncline cores,(2)cover coverage area,and(3) the deeper buried area where the range of tectonic uplift is small.The influences of tectonism,magmatic intrusion and uplift-erosion on the spatial distribution shapes of BIF are clarified.展开更多
The method of infrared thermography to predict the temperature of the sulfide ores has a large error. To solve this problem, the temperature of the sulfide ores is measured by thermal infrared imager and recording the...The method of infrared thermography to predict the temperature of the sulfide ores has a large error. To solve this problem, the temperature of the sulfide ores is measured by thermal infrared imager and recording thermometric instrument contrastively. The main factors, including emissivity, distance, angle and dust concentration that affect the temperature measurement precision, are analyzed. The regression equations about the individual factors and comprehensive factors are obtained by analyzing test data. The application of the regression equations improves the precision of the thermal infrared imager. The geometric information lost in traditional infrared thermometry is determined by visualization grid method and interpolation method, the relationship between the infrared imager and geometry information is established. The geometry location can be measured exactly.展开更多
To obtain the appropriate conditions for eliminating Fe3+from NiSO4 solution, the digestion solution of the clinker was used as raw material, which was obtained from roasting the nickel oxide ore with (NH4)2SO4. Th...To obtain the appropriate conditions for eliminating Fe3+from NiSO4 solution, the digestion solution of the clinker was used as raw material, which was obtained from roasting the nickel oxide ore with (NH4)2SO4. The ammonium jarosite was successfully synthesized from the solution with analytic grade NH4HCO3. The effects of reaction temperature, reaction time, end pH value of reaction on the removal rate of iron were investigated, and the effect of the initial concentration of Fe3+was also discussed. All of those factors had significant effects on the removal rate of Fe3+, among which the reaction temperature was the most prominent. The appropriate reaction conditions were concluded as follows: reaction temperature 95 ℃ reaction time 3.5 h, end pH value of reaction 2.5 at initial concentration of Fe3+19.36 g/L. The physical aspect of (NH4)2Fe6(SO4)4(OH)12 was cluster figure composed of sheet or prismatic particles with smooth surface.展开更多
The thermal decomposition kinetics of high iron gibbsite ore was investigated under non-isothermal conditions.Popescu method was applied to analyzing the thermal decomposition mechanism.The results show that the most ...The thermal decomposition kinetics of high iron gibbsite ore was investigated under non-isothermal conditions.Popescu method was applied to analyzing the thermal decomposition mechanism.The results show that the most probable thermal decomposition mechanism is the three-dimensional diffusion model of Jander equation,and the mechanism code is D3.The activation energy and pre-exponential factor for thermal decomposition of high iron gibbsite ore calculated by the Popescu method are 75.36 kJ/mol and 1.51×10-5 s-(-1),respectively.The correctness of the obtained mechanism function is validated by the activation energy acquired by the iso-conversional method.Popescu method is a rational and reliable method for the analysis of the thermal decomposition mechanism of high iron gibbsite ore.展开更多
In this study, the mass attenuation coefficient of boron-containing ores in the Liaoning province of China was calculated using Win XCOM software to investigate the shielding effectiveness of these ores against gamma ...In this study, the mass attenuation coefficient of boron-containing ores in the Liaoning province of China was calculated using Win XCOM software to investigate the shielding effectiveness of these ores against gamma rays. The mass attenuation coefficients were also calculated using MCNP-4 B code and compared with Win XCOM results; consequently, a good consistency between the results of Win XCOM and MCNP-4 B was observed. Furthermore, the G-P fitting method was used to evaluate the values of exposure buildup factor(EBF) in the energy range of 0.015–15 Me V up to 40 mean free paths. Among the selected ores, boron-bearing iron concentrate ore(M3)was determined to be the best gamma ray shielding ore owing to its higher values of mass attenuation coefficient and equivalent atomic number and lower value of EBF.Moreover, American Evaluated Nuclear Data File(ENDF/B-VII) was used to analyze the shielding effectivenessagainst thermal neutrons. It was determined that Szaibelyite(M2) is the best thermal neutron shielding material.This study would be useful for demonstrating the potential of boron-containing ores for applications in the field of nuclear engineering and technology.展开更多
The use of econometric methods to analyze the relationship between our country steel price index and the international iron ore freight rate,time series stationarity test,cointegration test,Granger test of causality a...The use of econometric methods to analyze the relationship between our country steel price index and the international iron ore freight rate,time series stationarity test,cointegration test,Granger test of causality and model parameter estimation tools use,find that there is Granger causality between our country steel price index and the international iron ore freight rate,China' s steel price fluctuations to some extent affect the international iron ore freight.展开更多
Based upon the aspect of continuous damaging,the process of ore comminution may be des- cribed as the growth and propagation of the microcracks in the ore grains under external load- ing,and a theoretical expression h...Based upon the aspect of continuous damaging,the process of ore comminution may be des- cribed as the growth and propagation of the microcracks in the ore grains under external load- ing,and a theoretical expression has been developed on the relationship between the grain size distribution and the total energy consumption during entire ore comminution process.The en- ergy consumed totally may be considered consisting of two portions,i.e.with fresh crack sur- faces growth and with crack propagation.The proposed expression seems to be an advance over previous conventional ones,especially that from the viewpoint of energy distribution.展开更多
Senescence is a highly regulated process that involves the action of a large number of transcription factors. The NAC transcription factor ORE1 (ANAC092) has recently been shown to play a critical role in positively...Senescence is a highly regulated process that involves the action of a large number of transcription factors. The NAC transcription factor ORE1 (ANAC092) has recently been shown to play a critical role in positively controlling senescence in Arabidopsis thaliana; however, no direct target gene through which it exerts its molecular function has been identified previously. Here, we report that BIFUNCTIONAL NUCLEASE1 (BFN1), a well-known senescence-enhanced gene, is directly regulated by ORE1. We detected elevated expression of BFN1 already 2 h after induction of ORE1 in estradiol-inducible ORE1 overexpression lines and 6 h after transfection of Arabidopsis mesophyll cell protoplasts with a 35S:ORE1 construct, ORE1 and BFN1 expression patterns largely overlap, as shown by promoter-reporter gene (GUS) fusions, while BFN1 expression in senescent leaves and the abscission zones of maturing flower organs was virtually absent in ore1 mutant background. In vitro binding site assays revealed a bipartite ORE1 binding site, similar to that of ORS1, a paralog of ORE1. A bipartite ORE1 binding site was identified in the BFN1 promoter; mutating the cis-element within the context of the full-length BFN1 promoter drastically reduced OREl-mediated transactivation capacity in tran- siently transfected Arabidopsis mesophyll cell protoplasts. Furthermore, chromatin immunoprecipitation (CHIP) demon- strates in vivo binding of ORE1 to the BFN1 promoter. We also demonstrate binding of ORE1 in vivo to the promoters of two other senescence-associated genes, namely SAG29/SWEET15 and SINA1, supporting the central role of ORE1 during senescence.展开更多
In order to clarify the solvent extraction and separation behaviors of rare earths and impurity of Al during the extraction and enrichment of low-concentration leach solution of ion-adsorption rare earth ore,the extra...In order to clarify the solvent extraction and separation behaviors of rare earths and impurity of Al during the extraction and enrichment of low-concentration leach solution of ion-adsorption rare earth ore,the extraction mechanism and separation behaviors of Nd^(3+)and Al^(3+)in the Nd_(2)(SO_(4))_(3)-AI_(2)(SO_(4))_(3) mixed solution using P507 were studied in this work.The extraction of Nd^(3+)and Al^(3+)follows the cation exchange mechanism.With the increase of the equilibrium pH,β_(Nd/Al) in the extraction of the Nd_(2)(SO_(4))_(3)-Al_(2)(SO_(4))_(3) mixed solution using P507 is always higher than that in the extraction of single Nd_(2)(SO_(4))_(3) and Al_(2)(SO_(4))_(3) solutions.It can be attributed to the fact that the extraction of Nd^(3+)using P507 is much faster than that of Al^(3+),and Al^(3+)is more prone to be hydrolyzed at lower pH.β_(Nd/Al) in the extraction of the Nd_(2)(SO_(4))_(3)-Al_(2)(SO_(4))_(3) mixed solution decreases gradually with the increase of mixing time within the equilibrium pH range of 1.5-1.9.The extraction of Nd^(3+)using P507 is much faster than that of Al^(3+),but the stability of Al^(3+)-Ioaded organic phase is better than that of Nd^(3+)-loaded organic phase,thus Nd^(3+)in the Nd^(3+)-loaded organic phase is gradually replaced by Al^(3+)in the aqueous phase with the increase of mixing time.展开更多
基金Project(2007CB416608) supported by the National Basic Research Program of ChinaProject(2006BAB01B07) supported by the National Science and Technology Pillar Program during the 11th Five-Year Plan Period
文摘To address the issues for assessing and prospecting the replaceable resource of crisis mines, a geological ore-controlling field model and a mineralization distribution field model were proposed from the viewpoint of field analysis. By dint of solving the field models through transferring the continuous models into the discrete ones, the relationship between the geological ore-controlling effect field and the mineralization distribution field was analyzed, and the quantitative and located parameters were extracted for describing the geological factors controlling mineralization enrichment. The method was applied to the 3-dimensional localization and quantitative prediction for concealed ore bodies in the depths and margins of the Daehang mine in Guangxi, China, and the 3-dimensional distribution models of mineralization indexes and ore-controlling factors such as magmatic rocks, strata, faults, lithology and folds were built. With the methods of statistical analysis and the non-linear programming, the quantitative index set of the geological ore-controlling factors was obtained. In addition, the stereoscopic located and quantitative prediction models were set up by exploring the relationship between the mineralization indexes and the geological ore-controlling factors. So far, some concealed ore bodies with the resource volume of a medium-sized mineral deposit are found in the deep parts of the Dachang Mine by means of the deep prospecting drills following the prediction results, from which the effectiveness of the predication models and results is proved.
基金financially supported by the National Key R&D Program of China(grant no.2017YFC0602405)the National Natural Science Foundation of China(grant no.42030811)。
文摘Rare metals including Lithium(Li),Beryllium(Be),Rubidium(Rb),Cesium(Cs),Zirconium(Zr),Hafnium(Hf),Niobium(Nb),Tantalum(Ta),Tungsten(W)and Tin(Sn)are important critical mineral resources.In China,rare metal mineral deposits are spatially distributed mainly in the Altay and Southern Great Xingán Range regions in the Central Asian orogenic belt;in the Middle Qilian,South Qinling and East Qinling mountains regions in the Qilian-Qinling-Dabie orogenic belt;in the Western Sichuan and Bailongshan-Dahongliutan regions in the Kunlun-Songpan-Garze orogenic belt,and in the Northeastern Jiangxi,Northwestern Jiangxi,and Southern Hunan regions in South China.Major ore-forming epochs include Indosinian(mostly 200-240 Ma,in particular in western China)and the Yanshanian(mostly 120-160 Ma,in particular in South China).In addition,Bayan Obo,Inner Mongolia,northeastern China,with a complex formation history,hosts the largest REE and Nb deposits in China.There are six major rare metal mineral deposit types in China:Highly fractionated granite;Pegmatite;Alkaline granite;Carbonatite and alkaline rock;Volcanic;and Hydrothermal types.Two further types,namely the Leptynite type and Breccia pipe type,have recently been discovered in China,and are represented by the Yushishan Nb-Ta-(Zr-Hf-REE)and the Weilasituo Li-Rb-Sn-W-Zn-Pb deposits.Several most important controlling factors for rare metal mineral deposits are discussed,including geochemical behaviors and sources of the rare metals,highly evolved magmatic fractionation,and structural controls such as the metamorphic core complex setting,with a revised conceptual model for the latter.
基金financially supported by the National Natural Science Foundation of China(No.U1260202)the Fundamental Research Funds for the Central Universities(No.FRF-MP-12-003B)
文摘The appropriate content and distribution of sinter moisture play an important role in the granulation of iron ores. In this study, the effects of porosity, size distribution, and particle shape on the water absorption rate (WAR) of four types of iron ores were analyzed by using the immersion method and capillary water absorption method. In addition, the mechanism underlying the water absorption process in iron ores was unraveled. It is found that the WARs of iron ores decrease quickly with the increase in water absorption time at the initial stages of water absorption. With further increase in absorption time, the WARs decrease gradually, until near 0. Iron ores with higher porosity, smaller particle size, and plate-like structure have the higher WARs. Compared with pores in the single-particle iron ore, voids among particles in the multi-particle iron oxide play an important role at the initial stages of water absorption. The water absorption mechanism of all single-particle and multi-particle iron ores analyzed in this study includes four steps, wherein the first three steps play a significant role in the sintering pro-cess.
基金financially supported by the Fundamental Research Funds for the Central Universities of China (No. FRF-IC-14-006)
文摘Sinter body strength, which reflects the strength of sinter, plays an important role in the improvement of sinter. In this study, the sinter body strengths of iron ores were measured using a microsintering method. The relationship between the chemical composition and sinter body strength was discussed. Moreover, sinter-pot tests were performed. The effects of sinter body strength on the sintering indexes were then elucidated, and the bottom limit of sinter body strength of blending ores was confirmed. In the results, the compressive strengths(CSs) of iron ores are observed to decrease with the increasing of the contents of loss on ignition(LOI), SiO 2, and Al2O3; however, LOI of less than 3wt% does not substantially influence the CSs of fine ores. In the case of similar mineral composition, the porosity, in particular, the ratio between the number of large pores and the total number of pores, strongly influences the sinter body strength. With an increase of the blending-ore CSs used in sinter-pot tests, the yield, productivity, and tumbler strength increase, and the solid fuel consumption decreases. The CSs of the blending ores only slightly affect the sintering time. The CS bottom limit of the blending ores is 310 N. When the CSs of the blending ores increase by 10%, the yield, productivity, and tumbler index increase by 1.9%, 2.8%, and 2.0%, respectively, and the solid fuel consumption decreases by 1.9%.
文摘A detailed investigation on 3D spatial distribution rules of Banded Iron-bearing Formation(BIF) with methods of gravity-magnetic inversion and 3D modeling of iron mine is presented based on the former analysis in the Anshan-Benxi area.Three dimension spatial distribution types of BIF are concluded as hook-like,tabularlike and "W"-like.BIF was mainly developed in three types of space including(1) the syncline cores,(2)cover coverage area,and(3) the deeper buried area where the range of tectonic uplift is small.The influences of tectonism,magmatic intrusion and uplift-erosion on the spatial distribution shapes of BIF are clarified.
基金Project (51074181) supported by the National Natural Science Foundation of ChinaProject (2010ssxt241) supported by Precious Dissertation Innovation Foundation of Central South University, China
文摘The method of infrared thermography to predict the temperature of the sulfide ores has a large error. To solve this problem, the temperature of the sulfide ores is measured by thermal infrared imager and recording thermometric instrument contrastively. The main factors, including emissivity, distance, angle and dust concentration that affect the temperature measurement precision, are analyzed. The regression equations about the individual factors and comprehensive factors are obtained by analyzing test data. The application of the regression equations improves the precision of the thermal infrared imager. The geometric information lost in traditional infrared thermometry is determined by visualization grid method and interpolation method, the relationship between the infrared imager and geometry information is established. The geometry location can be measured exactly.
基金Project(51204054)supported by the National Natural Science Foundation of ChinaProject(N110402012)supported by Fundamental Research Funds for the Central Universities,ChinaProject(2007CB613603)supported by the National Basic Research Program of China
文摘To obtain the appropriate conditions for eliminating Fe3+from NiSO4 solution, the digestion solution of the clinker was used as raw material, which was obtained from roasting the nickel oxide ore with (NH4)2SO4. The ammonium jarosite was successfully synthesized from the solution with analytic grade NH4HCO3. The effects of reaction temperature, reaction time, end pH value of reaction on the removal rate of iron were investigated, and the effect of the initial concentration of Fe3+was also discussed. All of those factors had significant effects on the removal rate of Fe3+, among which the reaction temperature was the most prominent. The appropriate reaction conditions were concluded as follows: reaction temperature 95 ℃ reaction time 3.5 h, end pH value of reaction 2.5 at initial concentration of Fe3+19.36 g/L. The physical aspect of (NH4)2Fe6(SO4)4(OH)12 was cluster figure composed of sheet or prismatic particles with smooth surface.
基金Project(51374058)supported by the National Natural Science Foundation of China
文摘The thermal decomposition kinetics of high iron gibbsite ore was investigated under non-isothermal conditions.Popescu method was applied to analyzing the thermal decomposition mechanism.The results show that the most probable thermal decomposition mechanism is the three-dimensional diffusion model of Jander equation,and the mechanism code is D3.The activation energy and pre-exponential factor for thermal decomposition of high iron gibbsite ore calculated by the Popescu method are 75.36 kJ/mol and 1.51×10-5 s-(-1),respectively.The correctness of the obtained mechanism function is validated by the activation energy acquired by the iso-conversional method.Popescu method is a rational and reliable method for the analysis of the thermal decomposition mechanism of high iron gibbsite ore.
基金supported by the National Natural Science Foundation of China(Nos.51472048,50774022)the Key Laboratory Project of Liaoning Province Education Office(No.LZ 2014-022)
文摘In this study, the mass attenuation coefficient of boron-containing ores in the Liaoning province of China was calculated using Win XCOM software to investigate the shielding effectiveness of these ores against gamma rays. The mass attenuation coefficients were also calculated using MCNP-4 B code and compared with Win XCOM results; consequently, a good consistency between the results of Win XCOM and MCNP-4 B was observed. Furthermore, the G-P fitting method was used to evaluate the values of exposure buildup factor(EBF) in the energy range of 0.015–15 Me V up to 40 mean free paths. Among the selected ores, boron-bearing iron concentrate ore(M3)was determined to be the best gamma ray shielding ore owing to its higher values of mass attenuation coefficient and equivalent atomic number and lower value of EBF.Moreover, American Evaluated Nuclear Data File(ENDF/B-VII) was used to analyze the shielding effectivenessagainst thermal neutrons. It was determined that Szaibelyite(M2) is the best thermal neutron shielding material.This study would be useful for demonstrating the potential of boron-containing ores for applications in the field of nuclear engineering and technology.
文摘The use of econometric methods to analyze the relationship between our country steel price index and the international iron ore freight rate,time series stationarity test,cointegration test,Granger test of causality and model parameter estimation tools use,find that there is Granger causality between our country steel price index and the international iron ore freight rate,China' s steel price fluctuations to some extent affect the international iron ore freight.
文摘Based upon the aspect of continuous damaging,the process of ore comminution may be des- cribed as the growth and propagation of the microcracks in the ore grains under external load- ing,and a theoretical expression has been developed on the relationship between the grain size distribution and the total energy consumption during entire ore comminution process.The en- ergy consumed totally may be considered consisting of two portions,i.e.with fresh crack sur- faces growth and with crack propagation.The proposed expression seems to be an advance over previous conventional ones,especially that from the viewpoint of energy distribution.
文摘Senescence is a highly regulated process that involves the action of a large number of transcription factors. The NAC transcription factor ORE1 (ANAC092) has recently been shown to play a critical role in positively controlling senescence in Arabidopsis thaliana; however, no direct target gene through which it exerts its molecular function has been identified previously. Here, we report that BIFUNCTIONAL NUCLEASE1 (BFN1), a well-known senescence-enhanced gene, is directly regulated by ORE1. We detected elevated expression of BFN1 already 2 h after induction of ORE1 in estradiol-inducible ORE1 overexpression lines and 6 h after transfection of Arabidopsis mesophyll cell protoplasts with a 35S:ORE1 construct, ORE1 and BFN1 expression patterns largely overlap, as shown by promoter-reporter gene (GUS) fusions, while BFN1 expression in senescent leaves and the abscission zones of maturing flower organs was virtually absent in ore1 mutant background. In vitro binding site assays revealed a bipartite ORE1 binding site, similar to that of ORS1, a paralog of ORE1. A bipartite ORE1 binding site was identified in the BFN1 promoter; mutating the cis-element within the context of the full-length BFN1 promoter drastically reduced OREl-mediated transactivation capacity in tran- siently transfected Arabidopsis mesophyll cell protoplasts. Furthermore, chromatin immunoprecipitation (CHIP) demon- strates in vivo binding of ORE1 to the BFN1 promoter. We also demonstrate binding of ORE1 in vivo to the promoters of two other senescence-associated genes, namely SAG29/SWEET15 and SINA1, supporting the central role of ORE1 during senescence.
基金Project supported by the Major Research Plan of the National Natural Science Foundation of China(91962211)National Key Research and Development Program of China(2018YFC1801803)+1 种基金National Natural Science Foundation of China(51804273)Major Project of Guangxi Science and Technology(Guike-AA18242022)。
文摘In order to clarify the solvent extraction and separation behaviors of rare earths and impurity of Al during the extraction and enrichment of low-concentration leach solution of ion-adsorption rare earth ore,the extraction mechanism and separation behaviors of Nd^(3+)and Al^(3+)in the Nd_(2)(SO_(4))_(3)-AI_(2)(SO_(4))_(3) mixed solution using P507 were studied in this work.The extraction of Nd^(3+)and Al^(3+)follows the cation exchange mechanism.With the increase of the equilibrium pH,β_(Nd/Al) in the extraction of the Nd_(2)(SO_(4))_(3)-Al_(2)(SO_(4))_(3) mixed solution using P507 is always higher than that in the extraction of single Nd_(2)(SO_(4))_(3) and Al_(2)(SO_(4))_(3) solutions.It can be attributed to the fact that the extraction of Nd^(3+)using P507 is much faster than that of Al^(3+),and Al^(3+)is more prone to be hydrolyzed at lower pH.β_(Nd/Al) in the extraction of the Nd_(2)(SO_(4))_(3)-Al_(2)(SO_(4))_(3) mixed solution decreases gradually with the increase of mixing time within the equilibrium pH range of 1.5-1.9.The extraction of Nd^(3+)using P507 is much faster than that of Al^(3+),but the stability of Al^(3+)-Ioaded organic phase is better than that of Nd^(3+)-loaded organic phase,thus Nd^(3+)in the Nd^(3+)-loaded organic phase is gradually replaced by Al^(3+)in the aqueous phase with the increase of mixing time.