In this editorial we comment on the article by Kuwahara et al,published in the recent issue of the World Journal of Cardiology.In this interesting paper,the authors showed a correlation between portal vein pulsatility...In this editorial we comment on the article by Kuwahara et al,published in the recent issue of the World Journal of Cardiology.In this interesting paper,the authors showed a correlation between portal vein pulsatility ratio,examined by bedside ultrasonography,and prognosis of hospitalized patients with acute heart failure.Systemic congestion is being notoriously underdetected in the acutely ill population with conventional methods like clinical examination,biomarkers,central venous pressure estimation and X-rays.However,congestion should be a key therapeutic target due to its deleterious effects to end organ function and subsequently patient prognosis.Doppler flow assessment of the abdominal veins is gaining popularity worldwide,as a valuable tool in estimating comprehensively congestion and giving a further insight into hemodynamics and patient management.展开更多
AIM:To evaluate and characterize motility patterns from small intestinal gut segments depending on different perfusion media and pressures.METHODS:Experiments were carried out in a custom designed perfusion chamber sy...AIM:To evaluate and characterize motility patterns from small intestinal gut segments depending on different perfusion media and pressures.METHODS:Experiments were carried out in a custom designed perfusion chamber system to validate and standardise the perfusion technique used.The perfusion chamber was built with a transparent front wall allowing for optical motility recordings and a custom made fastener to hold the intestinal segments.Experiments with different perfusion and storage media combined with different luminal pressures were carried out to evaluate the effects on rat small intestine motility.Software tools which enable thevisualization and characterization of intestinal motility in response to different stimuli were used to evaluate the videotaped experiments.The data collected was presented in so called heatmaps thus providing a concise overview of form and strength of contractility patterns.Furthermore,the effect of different storage media on tissue quality was evaluated.HaematoxylinEosin stainings were used to compare tissue quality depending on storage and perfusion mode.RESULTS:Intestinal motility is characterized by different repetitive motility patterns,depending on the actual situation of the gut.Different motility patterns could be recorded and characterized depending on the perfusion pressure and media used.We were able to describe at least three different repetitive patterns of intestinal motility in vitro.Patterns with an oral,anal and oro-anal propagation direction could be recorded.Each type of pattern finalized its movement with or without a subsequent distension of the wavefront.Motility patterns could clearly be distinguished in heatmap diagrams.Furthermore undirected motility could be observed.The quantity of the different patterns varies and is highly dependent on the perfusion medium used.Tissue preservation varies depending on the perfusion medium utilized,therefore media with a simple composition as Tyrode solution can only be recommended for short time experiments.The more complex media,MEM-HEPES medium and especially AQIXRS-I tissue preservation reagent preserved the tissue much better during perfusion.CONCLUSION:Perfusion media have to be carefully chosen considering type and duration of the experiments.If excellent tissue quality is required,complex media are favorable.Perfusion pressure is also of great importance due to the fact that a minimum amount of luminal pressure seems to be necessary to trigger intestinal contractions.展开更多
文摘In this editorial we comment on the article by Kuwahara et al,published in the recent issue of the World Journal of Cardiology.In this interesting paper,the authors showed a correlation between portal vein pulsatility ratio,examined by bedside ultrasonography,and prognosis of hospitalized patients with acute heart failure.Systemic congestion is being notoriously underdetected in the acutely ill population with conventional methods like clinical examination,biomarkers,central venous pressure estimation and X-rays.However,congestion should be a key therapeutic target due to its deleterious effects to end organ function and subsequently patient prognosis.Doppler flow assessment of the abdominal veins is gaining popularity worldwide,as a valuable tool in estimating comprehensively congestion and giving a further insight into hemodynamics and patient management.
文摘AIM:To evaluate and characterize motility patterns from small intestinal gut segments depending on different perfusion media and pressures.METHODS:Experiments were carried out in a custom designed perfusion chamber system to validate and standardise the perfusion technique used.The perfusion chamber was built with a transparent front wall allowing for optical motility recordings and a custom made fastener to hold the intestinal segments.Experiments with different perfusion and storage media combined with different luminal pressures were carried out to evaluate the effects on rat small intestine motility.Software tools which enable thevisualization and characterization of intestinal motility in response to different stimuli were used to evaluate the videotaped experiments.The data collected was presented in so called heatmaps thus providing a concise overview of form and strength of contractility patterns.Furthermore,the effect of different storage media on tissue quality was evaluated.HaematoxylinEosin stainings were used to compare tissue quality depending on storage and perfusion mode.RESULTS:Intestinal motility is characterized by different repetitive motility patterns,depending on the actual situation of the gut.Different motility patterns could be recorded and characterized depending on the perfusion pressure and media used.We were able to describe at least three different repetitive patterns of intestinal motility in vitro.Patterns with an oral,anal and oro-anal propagation direction could be recorded.Each type of pattern finalized its movement with or without a subsequent distension of the wavefront.Motility patterns could clearly be distinguished in heatmap diagrams.Furthermore undirected motility could be observed.The quantity of the different patterns varies and is highly dependent on the perfusion medium used.Tissue preservation varies depending on the perfusion medium utilized,therefore media with a simple composition as Tyrode solution can only be recommended for short time experiments.The more complex media,MEM-HEPES medium and especially AQIXRS-I tissue preservation reagent preserved the tissue much better during perfusion.CONCLUSION:Perfusion media have to be carefully chosen considering type and duration of the experiments.If excellent tissue quality is required,complex media are favorable.Perfusion pressure is also of great importance due to the fact that a minimum amount of luminal pressure seems to be necessary to trigger intestinal contractions.
