Origin软件在“微生物学”实验教学中的应用——以细菌生长曲线的测定实验为例

针对高校本科“微生物学”实验教学中遇到的数据处理问题,以细菌生长曲线测定的实验为例,使用Origin 2022软件进行数据导入、数据计算、图形绘制及插入误差棒等操作,完成细菌生长曲线的绘制。结果表明,利用Origin软件可以快速对实验数据进行批量处理,操作简捷并可避免人工计算的误差,对实验得到的非线性数据通过软件可以拟合出美观的图形,将实验结果可视化。在教学中通过引入Origin软件,可以在讲授具体微生物知识的同时,让本科生掌握一款专业的数据分析处理软件,有助于培养本科生的数据处理能力,提高综合科研素养,为后续实验课程和科研工作的数据处理工作打好基础。

Identification of S-RNase genotype and analysis of its origin and evolutionary patterns in Malus plants

Identification of the S genotype of Malus plants will greatly promote the discovery of new genes,the cultivation and production of apple,the breeding of new varieties,and the origin and evolution of self-incompatibility in Malus plants.In this experiment,88 Malus germplasm resources,such as Aihuahong,Xishuhaitang,and Reguanzi,were used as materials.Seven gene-specific primer combinations were used in the genotype identification.PCR amplification using leaf DNA produced a single S-RNase gene fragment in all materials.The results revealed that 70 of the identified materials obtained a complete S-RNase genotype,while only one S-RNase gene was found in 18 of them.Through homology comparison and analysis,13 S-RNase genotypes were obtained:S_(1)S_(2)(Aihuahong,etc.),S_(1)S_(28)(Xixian Haitang,etc.),S_(1)S_(51)(Hebei Pingdinghaitang),S_(1)S_(3)(Xiangyangcun Daguo,etc.),S_(2)S_(3)(Zhaiyehaitang,etc.),S_(3)S_(51)(Xishan 1),S_(3)S_(28)(Huangselihaerde,etc.),S_(2)S_(28)(Honghaitang,etc.),S_(4)S_(28)(Bo 11),S_(7)S_(28)(Jiuquan Shaguo),S_(10)S_e(Dongchengguan 13),S_(10)S_(21)(Dongxiangjiao)and S_(3)S_(51)(Xiongyue Haitang).Simultaneously,the frequency of the S gene in the tested materials was analyzed.The findings revealed that different S genes had varying frequencies in Malus resources,as well as varying frequencies between intraspecific and interspecific.S_(3) had the highest frequency of 68.18%,followed by S_(1)(42.04%).In addition,the phylogenetic tree and origin evolution analysis revealed that the S gene differentiation was completed prior to the formation of various apple species,that cultivated species also evolved new S genes,and that the S_(50) gene is the oldest S allele in Malus plants.The S_(1),S_(29),and S_(33) genes in apple-cultivated species,on the other hand,may have originated in M.sieversii,M.hupehensis,and M.kansuensis,respectively.In addition to M.sieversii,M.kansuensis and M.sikkimensis may have also played a role in the origin and evolution of some Chinese apples.

Geographical origin identification of winter jujube(Ziziphus jujuba Dongzao')by using multi-element fingerprinting with chemometrics

Winter jujube(Ziziphus jujuba'Dongzao')is greatly appreciated by consumers for its excellent quality,but brand infringement frequently occurs in the market.Here,we first determined a total of 38 elements in 167 winter jujube samples from the main winter jujube producing areas of China by inductively coupled plasma mass spectrometer(ICP-MS).As a result,16 elements(Mg,K,Mn,Cu,Zn,Mo,Ba,Be,As,Se,Cd,Sb,Ce,Er,Tl,and Pb)exhibited significant differences in samples from different producing areas.Supervised linear discriminant analysis(LDA)and orthogonal projection to latent structures discriminant analysis(OPLS-DA)showed better performance in identifying the origin of samples than unsupervised principal component analysis(PCA).LDA and OPLS-DA had a mean identification accuracy of 87.84 and 94.64%in the testing set,respectively.By using the multilayer perceptron(MLP)and C5.0,the prediction accuracy of the models could reach 96.36 and 91.06%,respectively.Based on the above four chemometric methods,Cd,Tl,Mo and Se were selected as the main variables and principal markers for the origin identification of winter jujube.Overall,this study demonstrates that it is practical and precise to identify the origin of winter jujube through multi-element fingerprint analysis with chemometrics,and may also provide reference for establishing the origin traceability system of other fruits.

On Promoting Listening Comprehension of English Majors through Original English Movies

In recent years,with the reform of College English Teaching in China,listening has occupied a great proportion of both the examination content and classroom teaching.When everyone is seeking a high-efficiency,fast teaching method,the use of English original film teaching methods arises spontaneously.Its authentic speaking style,real language environment and rich cultural connotation not only improve the listening comprehension ability of English majors,but also enable students to intuitively understand the culture of Western countries.In this study,by referring to a large number of books and documents,this paper puts forward corresponding strategies for various problems arising from the use of English original film teaching in colleges and universities.English original film teaching can not only create a better learning environment,and stimulate students’interest in learning,but also enable students to actively participate in class discussions.

Management of retroperitoneal high-grade serous carcinoma of unknown origin:A case report

BACKGROUND Retroperitoneal high-grade serous carcinoma(HGSC)of unknown origin is a sporadic tumor that can originate from ovarian cancer.Herein,we report the case of a woman with retroperitoneal HGSC of unknown origin and describe how she was diagnosed and treated.CASE SUMMARY A 71-year-old female presented with the tumor marker CA125 elevated to 1041.9 U/mL upon a regular health examination.Computed tomography revealed retroperitoneal lymph node enlargement.Subsequently,positron emission tomography scanning revealed lesions with increased F-18 fluorodeoxyglucose uptake at the nodes.As a result,she underwent laparoscopic lymph node resection,and pathology revealed metastatic adenocarcinoma with CK7(+),PAX8(+),WT1(+),PR(-),and p53 mutational loss of expression,indicating that the origin may be from the adnexa.The patient was admitted to our ward and underwent laparoscopic staging;however,the pathological results were negative.Under the suspicion of retroperitoneal HGSC of unknown origin,chemotherapy and targeted therapy were initiated.Tumor marker levels decreased after treatment.CONCLUSION We present a case of HGSC of unknown origin managed using retroperitoneal lymphadenectomy,staging surgery,chemotherapy,and targeted therapy.

Origin of the Dashuigou independent tellurium deposit at Qinghai–Xizang Plateau: constraints from the light stable isotopes C, O, and H

By studying the light isotopic compositions of carbon,oxygen,and hydrogen,combined with previous research results on the ore-forming source of the deposit,the authors try to uncover its metallogenic origin.The δ^(18)O and δ^(13)C isotope signatures of dolomite samples vary between 10.2 and 13.0‰,and between−7.2 and−5.2‰,respectively,implying that the carbon derives from the upper mantle.δD and δ^(18) O of quartz,biotite,and muscovite from diff erent ore veins of the deposit vary between−82 and−59‰,and between 11.6 and 12.4‰,respectively,implying that the metallogenic solutions are mainly magmatic.According to the relevant research results of many isotope geologists,the fractionation degree of hydrogen isotopes increases as the depth to the Earth’s core increases,and the more diff erentiated the hydrogen isotopes are,the lower their values will be.In other words,mantle-derived solutions can have extremely low hydrogen isotope values.This means that the δD‰ value−134 of the pyrrhotite sample numbered SD-34 in this article may indicate mantle-derived oreforming fl uid of the deposit.The formation of the Dashuigou tellurium deposit occurred between 91.71 and 80.19 Ma.

最小二乘法和Origin软件在牛顿第二运动定律验证实验的数据处理中的应用

用最小二乘法和Origin软件分别对牛顿第二运动定律验证实验的实验数据进行处理。计算结果表明,Origin软件在处理实验数据方面具有快速、灵活、便捷等优势,避免了因人为因素所造成的误差,因而在实验数据处理中具有广泛的应用前景。

汲取“伟人”智慧,创新“素养”提升--利用Tracker和 Origin软件探究自由落体运动

伽利略对于自由落体运动的研究囊括了数学推理、实验验证和思想外推.本文汲取“伟人”--伽利略的自由落体运动研究的思想,并以现代技术手段--Tracker软件获取数据和Origin软件处理数据为抓手,创新自由落体运动的探究过程,促进学生科学探究和科学思维等学科核心素养的提升,以期专家和同行指正.

vivo OriginOS 4系统深度体验报告

随着AI大模型热潮愈演愈烈以及Android14正式版的发布,最近不少手机厂商公布了操作系统的升级计划,如vivo在2023年11月1日举行的开发者大会上就正式官宣了OriginOS4系统。OriginOS历代记2020年11月,vivo基于Android底层和Linux内核研发的OriginOS1.0正式问世。根据vivo官方的说法,以“本原做设计,设计为本原”为原则,OriginOS希望达成的目的是成为“连接物理世界和数字世界的桥梁”。该系统一问世就受到了广泛好评,因为它一方面在视觉设计对过去千篇一律的Android系统UI进行了重构,带来了更符合时代审美的诸多创新设计,如其首创的桌面架构体系“华容网格”,对桌面元素进行了重构,让桌面上的很多基础单元(系统组件和图标)都能进行自定义布局组合。

用Origin软件探究HgCdTe单晶的变温霍尔效应

利用Origin软件的模板化绘图、列数据计算、App插件等功能,探究了HgCdTe单晶样品的本征激发温区、禁带宽度、副效应影响等特性.结果表明,Origin软件在处理实验数据方面具有便捷和高效的优势.

Imperial Leather Original皂用香精的仿制

介绍了Imperial Leather Original皂用香精的特点及其香韵组成,详细叙述了如何运用不同香韵的单体原料仿配该香精的过程。讨论了香精的稳定性试验和三角试验。

“Original Affluent Society”翻译刍议——兼与丘延亮先生商榷

萨林斯提出的"Original Affluent Society"是一个非常重要的经济人类学概念。从人类学对狩猎采集社会认识与表述的演变过程来看,"original society"不应译为具有一定文化价值标准的"原初社会",而应译成较为中性的"初民社会"。就经济状况而言,狩猎采集社会在本质上不是"丰裕社会",而是物产相对丰富、目标和欲求非常有限的"富饶社会"。因此,"Original Affluent Society"译成"初民富饶社会"比译为"原初丰裕社会"妥当。

Origin作图法研究灵敏电流计的特性

灵敏电流计允许通过的电流很小,实验中采用二级分压的方法,使其两端的电压很小。测量时采用变偏法,保持总电压U和分压电阻R_(2)不变,改变外电路电阻R,测得对应的偏转格数d。处理数据时,用Origin软件拟合R~1/d曲线,即可得出直线的斜率和截距,从而求出电流常数k和灵敏电流计的内阻R_(g)。相对于手工作图,Origin拟合数据更为快速,值得推广到大学物理实验课程的其他实验中去。

基于Origin的土工颗粒分析试验数据处理

分析了基于Origin软件处理颗粒、分析试验数据的原理,并以开封某地粉沙土颗粒分析试验结果为基础数据,探讨了其数据分析处理的步骤。最后,通过与Excel、AutoCAD软件绘制颗粒级配曲线的方法相比,验证了该方法的适用性。

Influence of original microstructure on the transformation behavior and mechanical properties of ultra-high-strength TRIP-aided steel

The transformation behavior and tensile properties of an ultra-high-strength transformation-induced plasticity （TRIP） steel （0.2C-2.0Si-I.SMn） were investigated by different heat treatments for automobile applications. The results show that F-TRIP steel, a tradi- tional TRIP steel containing as-cold-rolled ferfite and pearlite as the original microstructure, consists of equiaxed grains of intercritical ferrite surrounded by discrete particles of M/RA and B. In contrast, M-TRIP steel, a modified TRiP-aided steel with martensite as the original mi- crostlucture, containing full martensite as the original microstructure is comprised of lath-shaped grains of ferrite separated by lath-shaped martensite/retained austenite and bainite. Most of the austenite in F-TRIP steel is granular, while the austenite in M-TRIP steel is lath-shaped. The volume fraction of the retained austenite as well as its carbon content is lower in F-TRIP steel than in M-TRIP steel, and austenite grains in M-TRIP steel are much finer than those in F-TRIP steel. Therefore, M-TRIP steel was concluded to have a higher austenite stability, re- sulting in a lower transformation rate and consequently contributing to a higher elongation compared to F-TRIP steel. Work hardening be- havior is also discussed for both types of steel.

Original potential flotation technology for sulphide minerals

Original potential flotation (OPF) technology, which controls potential by chemical atomsphere resulted from various redox reactions in grinding flotation system, but neither through the use of redox reagents nor through the application of potentials from an external source, was proposed. For lead zinc iron sulphide minerals flotation system, the relationship among technological parameters such as pH, E op , collectors and flotaion time, was studied. The optimum conditions for the selective flotation of galena from the pb Zn Fe sulfide ores were: pH of 12.5～12.8,the pulp potential of 150～180 mV and with diethyldithioncarbamate (DDTC) as collector.

The Original Organism Assemblages and Kerogen Carbon Isotopic Compositions of the Early Paleozoic Source Rocks in the Tarim Basin, China

Original organisms are the biological precursors of organic matter in source rocks. Original organisms in source rocks are informative for oil-source rock correlation and hydrocarbon potential evaluation, especially for source rocks which have high-over level of thermal maturity. Systematic identification of original organism assemblages of the Lower Paleozoic potential source rocks and detailed carbon isotopic composition of kerogen analyses were conducted for four outcrop sections in the Tarim basin. Results indicated that the original organism assemblages of the lower part of the Lower Cambrian were composed mainly of benthic algae, whereas those of the Upper Cambrian and the Ordovician were characterized by planktonic algae. Kerogen carbon isotopic data demonstrated that the δ13 Ckerogen values of source rocks dominated by benthic algae are lower than-34‰, whereas the δ13 Ckerogen values of source rocks dominated by planktonic algae are higher than-30‰ in general. We tentatively suggested that the carbon species those are utilized by algae and the carbon isotopic fractionation during photosynthesis are the major controls for the δ13 Ckerogen values in the Lower Paleozoic source rocks in the Tarim basin. Correlating the δ13 C values of oils exploited in the Tarim basin, the original organism assemblages, and δ13 Ckerogen values of source rocks, it implied that the Lower Paleozoic oils exploited in the Tarim basin should be sourced from the source rocks with original organism assemblages dominated by planktonic algae, and the hydrocarbon sourced from the Cambrian benthic algae should be of great exploration potential in future. Original organism assemblages in source rocks can provide important clues for oil-source rocks correlation, especially for the source rocks with high thermal maturity.

Review on Connections for Original Bamboo Structures

Bamboo is a green construction material in line with sustainable development strategies.The use of raw bamboo in architecture has existed since ancient times.In the long development years of original bamboo buildings,many areas in the world gradually formed unique bamboo buildings,which have become an important local cultural feature.For building structures,joints are the key to ensure structural load transfer.Because of hollow and thin-walled material property of bamboo,the connection in raw bamboo buildings has always been a major difficulty and problem in the application of bamboo,which seriously hinders the development of original bamboo structures.In order to promote the use of raw bamboo,two traditional connection methods in raw bamboo structures are described in this paper firstly,with the advantages and disadvantages of the two methods pointed out.Also,research progress on four categories of raw bamboo building joints is described namely,bolt joints,steel member joints,filler reinforced joints and other types of joints.This work can provide a reference for future research and engineering applications.

Development of a Soil Stabilizer for Road Subgrade Based on Original Phosphogypsum

The research used industrial by-products original phosphogypsum(PG)as the main raw material,slag(SG)and Portland cement(PC)as auxiliary materials,and the optimal additive amount was determined according to the compressive strength value of the sample.Comprehensively evaluate the water resistance and volume stability of the samples,and determine the best formula for new roadbed stabilized materials.The results showed that when the weight ratio of PG,slag and cement was OPG:SG:PC=6:3:1,and mixed with 5%micro silica fume(MSF)and 3‰hydroxypropyl methyl cellulose(HPMC),the sample’s comprehensive performance was the best,specifically,the sample’s compressive strength in 60 days reached 28.8 MPa,the softening coefficient reached 0.9,and the expansion rate was stable at about−0.2%.In addition,the mechanism of action of enhancers MSF and HPMC was analyzed according to use Vicat device,X-ray diffractometer and scanning electron microscope.The best formula SP3GH3 has the best curing effect on soil.The 28-day unconfined compressive strength(UCS)of the sample reached 2.4 MPa,the expansion rate was less than 0.09%,and the water stability coefficient was above 0.79,which was higher than that of the samples cured by traditional cement and lime during the same period.

The telomere-to-telomere genome of Fragaria vesca reveals the genomic evolution of Fragaria and the origin of cultivated octoploid strawberry

Fragaria vesca,commonly known as wild or woodland strawberry,is the most widely distributed diploid Fragaria species and is native to Europe and Asia.Because of its small plant size,low heterozygosity,and relative ease of genetic transformation,F.vesca has been a model plant for fruit research since the publication of its Illumina-based genome in 2011.However,its genomic contribution to octoploid cultivated strawberry remains a long-standing question.Here,we de novo assembled and annotated a telomere-to-telomere,gap-free genome of F.vesca‘Hawaii 4’,with all seven chromosomes assembled into single contigs,providing the highest completeness and assembly quality to date.The gap-free genome is 220785082 bp in length and encodes 36173 protein-coding gene models,including 1153 newly annotated genes.All 14 telomeres and seven centromeres were annotated within the seven chromosomes.Among the three previously recognized wild diploid strawberry ancestors,F.vesca,F.iinumae,and F.viridis,phylogenomic analysis showed that F.vesca and F.viridis are the ancestors of the cultivated octoploid strawberry F.×ananassa,and F.vesca is its closest relative.Three subgenomes of F.×ananassa belong to the F.vesca group,and one is sister to F.viridis.We anticipate that this high-quality,telomere-to-telomere,gap-free F.vesca genome,combined with our phylogenomic inference of the origin of cultivated strawberry,will provide insight into the genomic evolution of Fragaria and facilitate strawberry genetics and molecular breeding.