Optimization of synergistic drug combinations of different sparganins against blood stasis syndrome

Background:Blood stasis syndrome is one of the major syndromes in the development of chronic conditions in traditional Chinese medicine.Blood stasis syndrome is closely related to microcirculation dysfunction and thrombosis.Trigonaceae,as a representative traditional Chinese medicine for promoting blood circulation and removing blood stasis,there are many studies on its anticoagulant,antiplatelet aggregation and antithrombotic effects.Methods:Optimization of sparganin compounds,comprising sparganin A,sparganin B,and sparganin C for the characterization of drug pair effects against blood stasis syndrome was carried out.Ternary quadratic regression orthogonal combination design was carried out in a mouse model of blood stasis syndrome.The concentrations of thromboxane B2,plasminogen activator inhibitor 1,fibrinogen,and endothelin 1 were evaluated by enzyme linked immunosorbent assay.Thymus,hepatic,and spleen indices were also assessed.The outcomes of the evaluations aided in identifying optimum parameter values for the most favorable precipitation against blood stasis syndrome.Subsequently,Cytoscape 3.7.1 was used for network pharmacology to predict the key targets of sparganins and disease.Results:After sparganin A,sparganin B,and sparganin C treatment,hepatic had a downward trend,while spleen indices and thymus had an upward trend.Compared with the model group,thromboxane B2,endothelin 1,plasminogen activator inhibitor 1,and fibrinogen were decreased(P<0.05).The blood stasis syndrome model regression equation was extremely significant,and the correlation coefficient of R was 0.939,indicating that sparganin A,sparganin B and sparganin C were positively correlated at all levels.According to the dosage of these three factors and test results,the equation was fitted and the maximum values of the three sparganin in the corresponding dose range were obtained as follows:A=0.2982 mg/10g,B=0.1438 mg/10g,C=0.0417 mg/10g.Thus,the optimum parameters were found to be 0.298:0.144:0.042 for synergistic drug combinations of sparganin compounds sparganin A,sparganin B,and sparganin C in blood stasis syndrome.The possible key targets of sparganins included matrix metalloproteinase 9,plasminogen,proto-oncogene tyrosine-protein kinase src,and akt serine/threonine kinase 1 in blood stasis syndrome.Conclusion:Our study for the first time found two novel cyclic peptide compounds sparganin B and sparganin C have an anti-blood stasis effect,and speculated sparganins key targets included matrix metalloproteinase 9,plasminogen,proto-oncogene tyrosine-protein kinase src,and akt serine/threonine kinase 1 in blood stasis syndrome.

Effect of Cultivation Conditionss on Quality Parameters of High Grade Hybrid Rape of Brassica napus L.

[Objective] This study aimed to investigate the combined effects of sowing date, plant density, nitrogenous fertilizer, phosphorus fertilizer and potassium fertilizer on quality parameters of high grade hybrid rapeseed. [Method] By using Youyan 599 and Sanbei 98 as experimental materials, quadratic orthogonal rotation combination with five factors was designed for this study. [Result] Erucic acid content was nega- tively correlated with sowing date and nitrogen fertilizer, positively correlated with plant density. Glucosinolate content was negatively correlated with sowing date, positively correlated with potash fertilizer amount. Oil content was negatively correlated with sowing date and nitrogen fertilizer amount, positively correlated with plant density and potash fertilizer amount. Protein content was positively correlated with sowing date and nitrogen fertilizer amount, negatively correlated with plant density and potash fer- tilizer amount. Oleic acid content was negatively correlated with phosphate fertilizer amount, positively correlated with nitrogen fertilizer amount. Palmitic acid was nega- tively correlated with potash fertilizer amount, positively correlated with sowing date, plant density, nitrogen fertilizer amount and phosphate fertilizer amount in Youyan 599, but negatively correlated with sowing date, plant density, nitrogen fertilizer amount and phosphate fertilizer amount in Sanbei 98. Stearic acid content was posi- tively correlated with sowing date, plant density, nitrogen fertilizer amount and phos- phate fertilizer amount. Linoleic acid was positively correlated with density and nitroge- nous fertilizer, but negatively correlated with potash fertilizer amount. Linolenic acid was positively correlated with nitrogen fertilizer amount and potash fertilizer amount. Eicosenoic acid was negatively correlated with sowing date. There were positive cor- relations and negative correlations among cultivation conditions and quality parame- ters, and the positive correlations and negative correlations could counteract each other. Expression of quality parameters for each treatment was the combined effect. Among the 50 pairs of simple correlations of five cultivation factors and ten quality parameters, 26 pairs were consistent between two varieties, 24 pairs of simple corre- lation were not. Sowing date, plant density, nitrogen fertilizer amount and phosphate fertilizer amount showed significant influence on erucic acid of two varieties, but small effect on palmitic acid. [Conclusion] The combined effect of the five cultivation factors could not fundamentally improve the quality traits of high grade hybrid rapeseed.

Effects of Mixed Fertilizer Applications on Hay Yield of Alfalfa

[ Objective] To study the effects of combined application of different elements on alfalfa hay yield. [ Medthod] Potassium sulfate, ammonium chloride, superphosphate and magnesium oxide were applied to alfalfa field in the experiment, and quaternary quadratic regression orthogonal combination was used to examine the effects of the experiment factors on hay yield of alfalfa. [ Result] The order of targets based on the effects on hay yield is as follows： potassium sulfate 〉 ammonium chloride 〉 magnesium oxide 〉 superphosphate. Among them, the potassium sulfate had a significant impact on alfalfa hay yield. The interaction between potassium sulfate and magnesium oxide also had a significant impact on alfalfa hay yield. [ Conclusion] The optimal cultivation measures for alfalfa hay yield of more than 19 224.6 kg/hm^2 are as follows： potassium sulfate, 163.253 - 181.679 kg/hm^2 ; ammonium chloride, 155.877 - 174.258 kg/hm^2 ; superphosphate, 566.177 - 632.924 kg/hm^2 ; and magnesium oxide, 30.060 - 33.628 kg/hm^2.

Nonsingularity of the Combinations of Two Orthogonal Projectors

The invertibility of combinations of two orthogonal projectors aP ＋ bQ - cPQ - dQP is researched by using the CSdecomposition of matrices and properties of orthogonal projectors. The Moore-enrose inverse of the combinations is presented under some special conditions.

Effects Analysis of Mg^2＋, dNTPs and Taq DNA Polymerase on SSR-PCR System of Pear

Effects of Mg^2＋, NTPs and Taq DNA polymerase in pear SSR-PCR system were analyzed by quadratic regressive orthogonal rotational combinational design. Results showed： absolute IOD （Integrated OD of each band） value of target band reduced with concentration rising of Mg^2＋ and Taq DNA polymerase, but heightened with concentration rising of dNTPs. The decay rate of absolute IOD value increased progressively with rising Mg^2＋ concentration, decreased gradually with rising Taq DNA polymerase concentration; the rising speed would be slower than the dNTPs increase. Absolute IOD value would reduce with concentration rising of dNTPs at a low level of Mg^2＋ concentration. Conversely it would rise with the increase of dNTPs while high Mg^2＋ concentration. Absolute IOD value would generally rise with concentration rising of Taq DNA polymerase while low Mg^2＋ concentration. On the contrary it would reduce with concentration rising of Taq DNA polymerase while high Mg^2＋ concentration.

Numerical Simulation of Atmosphere-Ocean-Sea Ice Interaction During Interannual Cycle in High Northern Latitudes

The interannual atmosphere-ocean-sea ice interaction （AOSI） in high northern latitudes is studied with a global atmosphere-ocean-sea ice coupled model system, in which the model components of atmosphere and land surface are from China National Climate Center and that of ocean and sea ice are from LASG, Institute of Atmospheric Physics, Chinese Academy of Sciences. A daily flux anomaly correction scheme is employed to couple the atmosphere model and the ocean model with the effect of inhomogenity of sea ice in high latitudes is considered. The coupled model system has been run for 50 yr and the results of the last 30 years are analyzed. After the sea level pressure （SLP）, surface air temperature （SAT）, sea surface temperature （SST）, sea ice concentration （SIC）, and sea surface sensible heat flux （SHF） are filtered with a digital filter firstly, their normalized anomalies are used to perform the decomposition of combined complex empirical orthogonal function （CCEOF） and then they are reconstructed with the leading mode. The atmosphere-ocean-sea ice interactions in high northern latitudes during a periodical cycle （approximately 4 yr） are analyzed. It is shown that： （1） When the North Atlantic Oscillation （NAO） is in its positive phase, the southerly anomaly appears in the Greenland Sea, SAT increases, the sea loses less SHF, SST increases and SIC decreases accordingly; when the NAO is in its negative phase, the northerly anomaly appears in the Greenland Sea, SAT decreases, the sea loses more SHF, SST decreases and SIC increases accordingly. There are similar features in the Barents Sea, but the phase of evolution in the Barents Sea is different from that in the Greenland Sea. （2） For an average of multi-years, there is a cold center in the inner part of the Arctic Ocean near the North Pole. When there is an anomaly of low pressure, which is closer to the Pacific Ocean, in the inner part of the Arctic Ocean, anomalies of warm advection appear in the region near the Pacific Ocean and anomalies of cold advection appear in the region near the Atlantic Ocean. Accompanying with these anomalies of warm and cold advection in these two regions~ warm and cold anomalies appear respectively. Accordingly, SHF sent to the atmosphere from the sea surface decreases and increases, and SST increases and decreases~ SIC decreases and increases in these two regions. When there is an anomaly of high pressure in the inner part of the Arctic Ocean, the former relationships reverse. From these results~ it can be deduced that, during the interannual cycle of the coupled atmosphere-ocean system, the variability of large-scale atmospheric circulation plays a dominant role and variations of SST and SIC are mainly responding to that of atmospheric circulation.

Estimation of Fired Polynomial Effects of Mixed Models Based on PBIB

This paper discusses the estimation of fixed polynomial effects of mixed models based on PBIB, gives three concrete estimation, and analyses the condition of the design block while is orthogonal in these models. It also shows in mixed models that the three estimations of fixed polynomial effects T are identical under tile fact that the design block is orthogonal.