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Storage stability of SBS modified bitumen based on mixed-level orthogonal array design
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作者 杨群 何文华 《Journal of Southeast University(English Edition)》 EI CAS 2012年第3期337-343,共7页
The styrene-butadiene-styrene(SBS) modified bitumens with different contents of SBS modifiers are stored in different conditions to study the storage stability of SBS modified bitumen.Mixed-level orthogonal array de... The styrene-butadiene-styrene(SBS) modified bitumens with different contents of SBS modifiers are stored in different conditions to study the storage stability of SBS modified bitumen.Mixed-level orthogonal array design(OAD) is used and factors such as SBS modifier content,storage time,storage temperature and container size are chosen in a mixed-level OAD with an OA16(31×44) matrix.Parameters like the separation softening point difference(the separation difference of the ring and ball softening point of the top and bottom samples) and the average softening point(the arithmetic mean of the softening points of the top and bottom samples) are proposed to evaluate the separation and the ageing of modified bitumen during storage in this experiment,respectively.The results reveal that the separation and the ageing during storage exhibit a complicated variation for storage temperature and time.The separation softening point difference decreases with the storage temperature rising from 20 to 120 ℃ and increases with the temperature exceeding 120 ℃,and the average softening point drops with the storage time being prolonged.Different storage conditions have various effects on the storage stability of SBS modified bitumen. 展开更多
关键词 storage stability mixed-level orthogonal array design separation softening point difference average softening point
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Optimal Design of High-Speed Partial Flow Pumps using Orthogonal Tests and Numerical Simulations
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作者 Jiaqiong Wang Tao Yang +2 位作者 Chen Hu Yu Zhang Ling Zhou 《Fluid Dynamics & Materials Processing》 EI 2024年第6期1203-1218,共16页
To investigate the influence of structural parameters on the performances and internal flow characteristics of partial flow pumps at a low specific speed of 10000 rpm,special attention was paid to the first and second... To investigate the influence of structural parameters on the performances and internal flow characteristics of partial flow pumps at a low specific speed of 10000 rpm,special attention was paid to the first and second stage impeller guide vanes.Moreover,the impeller blade outlet width,impeller inlet diameter,blade inclination angle,and number of blades were considered for orthogonal tests.Accordingly,nine groups of design solutions were formed,and then used as a basis for the execution of numerical simulations(CFD)aimed at obtaining the efficiency values and heads for each design solution group.The influence of impeller geometric parameters on the efficiency and head was explored,and the“weight”of each factor was obtained via a range analysis.Optimal structural parameters were finally chosen on the basis of the numerical simulation results,and the performances of the optimized model were verified accordingly(yet by means of CFD).Evidence is provided that the increase in the efficiency and head of the optimized model was 12.11%and 23.5 m,respectively,compared with those of the original model. 展开更多
关键词 HIGH-SPEED partial flow pump orthogonal test optimal design numerical calculation
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Optimization of Center of Gravity Position and Anti-Wave Plate Angle of Amphibious Unmanned Vehicle Based on Orthogonal Experimental Method
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作者 Deyong Shang Xi Zhang +3 位作者 Fengqi Liang Chunde Zhai Hang Yang Yanqi Niu 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第5期2027-2041,共15页
When the amphibious vehicle navigates in water,the angle of the anti-wave plate and the position of the center of gravity greatly influence the navigation characteristics.In the relevant research on reducing the navig... When the amphibious vehicle navigates in water,the angle of the anti-wave plate and the position of the center of gravity greatly influence the navigation characteristics.In the relevant research on reducing the navigation resistance of amphibious vehicles by adjusting the angle of the anti-wave plate,there is a lack of scientific selection of parameters and reasonable research of simulation results by using mathematical methods,and the influence of the center of gravity position on navigation characteristics is not considered at the same time.To study the influence of the combinations of the angle of the anti-wave plate and the position of the center of gravity on the resistance reduction characteristics,a numerical calculation model of the amphibious unmanned vehicle was established by using the theory of computational fluid dynamics,and the experimental data verified the correctness of the numerical model.Based on this numerical model,the navigation characteristics of the amphibious unmanned vehicle were studied when the center of gravity was located at different positions,and the orthogonal experimental design method was used to optimize the parameters of the angle of the anti-wave plate and the position of the center of gravity.The results show that through the parameter optimization analysis based on the orthogonal experimental method,the combination of the optimal angle of the anti-wave plate and the position of the center of gravity is obtained.And the numerical simulation result of resistance is consistent with the predicted optimal solution.Compared with the maximum navigational resistance,the parameter optimization reduces the navigational resistance of the amphibious unmanned vehicle by 24%. 展开更多
关键词 Amphibious unmanned vehicle orthogonal experimental design anti-wave plate center of gravity resistance characteristic
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Study on Rapid Propagation Medium of Atractylodes Lances by Orthogonal Design 被引量:1
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作者 李文 谢烨明 +2 位作者 罗翠平 李金华 曾万勇 《Agricultural Science & Technology》 CAS 2012年第1期49-52,共4页
[Objective] The aim was to protect wild resource of Atractylodes lances and to provide technical guidance for industrialization of Atractylodes lances. [Method] The test-tube seedlings were produced from seeds of Atra... [Objective] The aim was to protect wild resource of Atractylodes lances and to provide technical guidance for industrialization of Atractylodes lances. [Method] The test-tube seedlings were produced from seeds of Atractylodis Lances. At the same time, the effects of 6-BA, NAA and KT on the proliferation of aseptic seedling was studied through the orthogonal test. Effect of NAA concentration on the rooting of aseptic seedling was studied through nine types of light substrate groups, which consist of peat soil, fine sand and cotton seed hull, and their effects on Atractylodes lances growth were studied on with orthogonal design. [Result] The culture medium suitable for the proliferation of the Atractylodes lances tube seedling was MS+6-BA 1.0 mg/L+NAA 0.4 mg/L+KT 0,4 mg/L. And the medium suitable for rooting was 1/2MS+NAA 0.5 mg/L. The optimal ratio of soil, riversand and cotton seed hull was 3:3:2. [Conclusion] The research selected optimum culture medium, rooting medium and ratio of substrate, which provided technical guidance for laboratorial preservation of germplasm resource of Atractylodes lances and industrialization of its seedling production. 展开更多
关键词 Atractylodes lance(Thunb.)DC. orthogonal design HORMONE SUBSTRATE
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Optimization by orthogonal array design of ion-pair HPLC separation of the enzymatic hydrolysis products of yeast RNA 被引量:3
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作者 Jie Hua Ke Long Huang 《Chinese Chemical Letters》 SCIE CAS CSCD 2010年第12期1453-1456,共4页
The separation of enzymatic hydrolysis products of yeast RNA by ion-pair HPLC was studied.A modified chromatographic response function(MCRF) was proposed to appraise the effectiveness of chromatographic separation.T... The separation of enzymatic hydrolysis products of yeast RNA by ion-pair HPLC was studied.A modified chromatographic response function(MCRF) was proposed to appraise the effectiveness of chromatographic separation.This function takes the number of peaks,resolution and the retention time of the last peak into consideration.It shows advantages for optimization of HPLC separation of complex mixtures.An orthogonal array design was used to separate the hydrolysate of yeast RNA and the optimal chromatographic conditions were obtained. 展开更多
关键词 Chromatographic response function orthogonal array design 5'-Phosphodiesterase Barley roots NUCLEOTIDES
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Modeling Approach and Analysis of the Structural Parameters of an Inductively Coupled Plasma Etcher Based on a Regression Orthogonal Design 被引量:3
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作者 程嘉 朱煜 季林红 《Plasma Science and Technology》 SCIE EI CAS CSCD 2012年第12期1059-1068,共10页
The geometry of an inductively coupled plasma (ICP) etcher is usually considered to be an important factor for determining both plasma and process uniformity over a large wafer. During the past few decades, these pa... The geometry of an inductively coupled plasma (ICP) etcher is usually considered to be an important factor for determining both plasma and process uniformity over a large wafer. During the past few decades, these parameters were determined by the "trial and error" method, resulting in wastes of time and funds. In this paper, a new approach of regression orthogonal design with plasma simulation experiments is proposed to investigate the sensitivity of the structural parameters on the uniformity of plasma characteristics. The tool for simulating plasma is CFD-ACE+, which is commercial multi-physical modeling software that has been proven to be accurate for plasma simulation. The simulated experimental results are analyzed to get a regression equation on three structural parameters. Through this equation, engineers can compute the uniformity of the electron number density rapidly without modeling by CFD-ACE+. An optimization performed at the end produces good results. 展开更多
关键词 fluid model inductively coupled plasma regression orthogonal structural parameters design of experiment
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Application of orthogonal experimental design and Tikhonov regularization method for the identification of parameters in the casting solidification process 被引量:4
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作者 Dashan SUI Zhenshan CUI 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2009年第1期13-21,共9页
The inverse heat conduction method is one of methods to identify the casting simulation parameters. A new inverse method was presented according to the Tikhonov regularization theory. One appropriate regularized funct... The inverse heat conduction method is one of methods to identify the casting simulation parameters. A new inverse method was presented according to the Tikhonov regularization theory. One appropriate regularized functional was established, and the functional was solved by the sensitivity coefficient and Newtonaphson iteration method. Moreover, the orthogonal experimental design was used to estimate the appropriate initial value and variation domain of each variable to decrease the number of iteration and improve the identification accuracy and efficiency. It illustrated a detailed case of AlSiTMg sand mold casting and the temperature measurement experiment was done. The physical properties of sand mold and the interracial heat transfer coefficient were identified at the meantime. The results indicated that the new regularization method was efficient in overcoming the ill-posedness of the inverse heat conduction problem and improving the stability and accuracy of the solutions. 展开更多
关键词 orthogonal experimental design Regularization method Parameters identification Numerical simulation
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Mathematical model for abrasive suspension jet cutting based on orthogonal test design 被引量:5
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作者 胡贵华 朱文华 +5 位作者 蔡红霞 徐翀 柏余杰 程俊 苑进 俞涛 《Journal of Shanghai University(English Edition)》 CAS 2009年第1期37-44,共8页
This paper describes the application of orthogonal test design coupled with non-linear regression analysis to optimize abrasive suspension jet (AS J) cutting process and construct its cutting model. Orthogonal test ... This paper describes the application of orthogonal test design coupled with non-linear regression analysis to optimize abrasive suspension jet (AS J) cutting process and construct its cutting model. Orthogonal test design is applied to cutting stainless steel. Through range analysis on experiment results, the optimal process conditions for the cutting depth and the kerr ratio of the bottom width to the top width can be determined. In addition, the analysis of ranges and variances are all employed to identify various factors: traverse rate, working pressure, nozzle diameter, standoff distance which denote the importance order of the cutting parameters affecting cutting depth and the kerf ratio of the bottom width to the top width. ~rthermore, non-linear regression analysis is used to establish the mathematical models of the cutting parameters based on the cutting depth and the kerr ratio. Finally, the verification experiments of cutting parameters' effect on cutting performance, which show that optimized cutting parameters and cutting model can significantly improve the prediction of the cutting ability and quality of ASJ. 展开更多
关键词 abrasive suspension jet (AS J) orthogonal test design cutting depth the kerf ratio of the bottom width to the top width non-linear regression analysis verification experiment
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Key design parameters and optimum method of medium- and high-velocity synchronous induction coilgun based on orthogonal experimental design 被引量:1
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作者 Kai-Pei Liu Xiao-Bo Niu +3 位作者 Ya-Dong Zhang Zeng-Chao Ji Jian-Yuan Feng Wen-Qi Li 《Chinese Physics B》 SCIE EI CAS CSCD 2019年第2期202-209,共8页
The energy conversion efficiency of a multistage synchronous induction coilgun(MSSICG) has become one of the key factors that restricts its industrialization. To improve the launch efficiency of medium-and high-veloci... The energy conversion efficiency of a multistage synchronous induction coilgun(MSSICG) has become one of the key factors that restricts its industrialization. To improve the launch efficiency of medium-and high-velocity MSSICG,we propose an optimization design scheme combining orthogonal experimental design(OED) and self-consistent design method in this paper. The OED is introduced to reduce the number of iterations and improve the identification accuracy and efficiency. A self-consistent design model is established to overcome a defect that the parameters that need to be optimized will multiply as the number of coil stages increases. The influence of six factors(radial thickness of armature, axial length of armature, axial length of coil, capacitance, wire diameter, and slip speed) on the launch efficiency are then evaluated by range analysis. This work presents a valuable reference for optimizing medium-and high-velocity MSSICG. 展开更多
关键词 MULTISTAGE synchronous induction coilgun(MSSICG) SELF-CONSISTENT design orthogonal experimental design(OED) range analysis
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Influence of radial forging process on strain inhomogeneity of hollow gear shaft using finite element method and orthogonal design 被引量:3
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作者 LI Hong-xu WANG Kai +5 位作者 LUO Rong ZHU Zi-zong DENG Shuai LUO Rong ZHANG Jing-yi FANG Fei-song 《Journal of Central South University》 SCIE EI CAS CSCD 2020年第6期1666-1677,共12页
Due to the current trend towards lightweight design in automotive industry,hollow stepped gear shafts for automobile and its radial forging process are widely investigated.Utilizing coupled finite element thermo-mecha... Due to the current trend towards lightweight design in automotive industry,hollow stepped gear shafts for automobile and its radial forging process are widely investigated.Utilizing coupled finite element thermo-mechanical model,radial forging process of a hollow stepped gear shaft for automobile was simulated.The optimal combination of three process parameters including initial temperature,rotation rate and radial reduction was also selected using orthogonal design method.To examine the strain inhomogeneity of the forging workpiece,the strain inhomogeneity factor was introduced.The results reveal that the maximum effective strain and the minimum effective strain appeared in the outermost and innermost zones of different cross sections for the hollow stepped gear shaft,respectively.Optimal forging parameters are determined as a combination of initial temperature of 780°C,rotation rate of 21°/stroke and radial reduction of 3 mm. 展开更多
关键词 radial forging process strain inhomogeneity orthogonal design coupled thermo-mechanical analysis finite element method
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Orthogonal discrete frequency coding waveform design based on chaos 被引量:1
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作者 Yuxing Peng Jin Yang +1 位作者 Zhaokun Qiu Xiang Li 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2013年第4期617-624,共8页
Orthogonal waveform design is quite an important issue for waveform diversity systems. A chaos based method for the orthogonal discrete frequency coding waveform (DFCW) design is proposed to increase the insufficien... Orthogonal waveform design is quite an important issue for waveform diversity systems. A chaos based method for the orthogonal discrete frequency coding waveform (DFCW) design is proposed to increase the insufficient orthogonal waveform number and their finite coding length. Premises for chaos choosing and the frequency quantification method are discussed to obtain the best correlation properties. Simulation results show the validity of the theoretic analysis. 展开更多
关键词 orthogonal waveform design waveform diversity CHAOS chaotic radar.
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Optimization for Microbial Degumming of Ramie with Bacillus subtilis DZ_(5) in Submerged Fermentation by Orthogonal Array Design and Response Surface Methodology 被引量:1
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作者 刘芬 陈杨栋 +2 位作者 朱鹏 曹张军 张兴群 《Journal of Donghua University(English Edition)》 CAS 2023年第5期475-481,共7页
As a kind of natural fiber,ramie fiber has distinctive advantages in textile application,but the application is limited due to the traditional degumming mode.Compared with the traditional degumming process,the microbi... As a kind of natural fiber,ramie fiber has distinctive advantages in textile application,but the application is limited due to the traditional degumming mode.Compared with the traditional degumming process,the microbial degumming process has many advantages.To obtain the optimal conditions for degumming ramie with Bacillus subtilis DZ_(5)(BS DZ_(5)),a combined statistical approach of orthogonal array design(OAD)and response surface methodology(RSM)was used.The influences of initial pH of the bacteria medium,culture temperature,shaking speed,degumming time and inoculum size on submerged fermentation degumming were evaluated by using fractional factorial design.The main factors in the analysis were culture temperature,shaking speed and initial pH.The residual gum mass fraction was used as the optimization index,and the optimal conditions for degumming were determined by central composite design and RSM.Thus with only a limited number of experiments,an optimal ramie microbial degumming condition was found as the culture temperature of 40℃,the initial pH in the culture medium of 8.5,the shaking speed of 205 r/min,the degumming time of 96 h and the inoculum size of 5%.After microbial degumming of ramie under the optimal conditions,there was only 10.6%residual gum by mass in the fiber.In addition,the effective degumming of BS DZ_(5)was also confirmed by a scanning electron microscope(SEM). 展开更多
关键词 microbial degumming OPTIMIZATION RAMIE orthogonal array design(OAD) response surface methodology(RSM)
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Optimized design of biconical liner by orthogonal method
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作者 曲忠伟 颜事龙 +1 位作者 沈兆武 马宏昊 《Journal of Beijing Institute of Technology》 EI CAS 2015年第1期8-17,共10页
A biconical liner was optimized to improve its penetration ability. Its formation and pene- tration mechanism was studied through numerical simulation and experiments. And the influence of different liner geometry siz... A biconical liner was optimized to improve its penetration ability. Its formation and pene- tration mechanism was studied through numerical simulation and experiments. And the influence of different liner geometry sizes on the jet performance was analyzed using the orthogonal method. The liner formed the high-speed jet with an explosively formed projectile. The small angle 2a remarkably influenced the jet speed which was inversely proportional to 2a. And the liner thickness t' and large angle 2β had highly significant effect on the projectile speed. The liner was optimized at t' = 0. 14 cm, 2α = 50°, 2β = 135°, N = 0. 4 or 0. 5, when its jet speed respectively is at 6 613 m/s and 6 839 m/s and projectile speed is at 2 247 m/s and 2 095 m/s, steel target penetration is at 8. 24 cm and 8. 31 cm, and aperture is in 2.12 cm and 2.08 cm. The results show that target is penetrated by the high-speed jet and high-speed projectile resulting into double damages. The penetration ability is improved greatly. 展开更多
关键词 biconical liner orthogonal design numerical simulation JET explosively formed pro-jectile
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Space-time Block Codes Based on Quasi-Orthogonal Designs
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作者 李正权 胡光锐 单红梅 《Journal of Donghua University(English Edition)》 EI CAS 2004年第4期4-7,共4页
A new space-time block codes based on quasi-orthogonal designs are put forward. First the channel model is formulated. Then the connection between orthogonal /quasi-orthogonal designs and space-time block codes is exp... A new space-time block codes based on quasi-orthogonal designs are put forward. First the channel model is formulated. Then the connection between orthogonal /quasi-orthogonal designs and space-time block codes is explored. Finally we make simulations for the transmission of 4 bits/s/Hz and 6 bits/s/Hz using eight transmit antennas using the rate 3/4 quasi-orthogonal space-time block code and the rate 1/2 full-diversity orthogonal space-time block code. Simulation results show that full transmission rate is more important for very low signal noise ratio (SNR) and high bit error probability (BEP), while full diversity is more important for very high SNR and low BEP. 展开更多
关键词 space-time block code orthogonal design quasi-orthogonal.
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Differential space-time modulation based on orthogonal design for cooperative network
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作者 丁盛 《High Technology Letters》 EI CAS 2008年第4期359-363,共5页
Differential modulation was widely used for wireless networks in which channel estimation was diffi-cult.Based on orthogonal design,a novel distributed differential space-time coding/decoding scheme forM-PSK modulatio... Differential modulation was widely used for wireless networks in which channel estimation was diffi-cult.Based on orthogonal design,a novel distributed differential space-time coding/decoding scheme forM-PSK modulations was proposed,which had a high code rate of 2/3 and second-order diversity for thetwo-user cooperative networks.The performance of decode-and-forward (DF) protocols was evaluated.Simulations show that the differential space-time modulation scheme in this paper has better bit error rate(BER) performance or higher code rate than the schemes proposed by Tarasak and Wang when interuserchannel states are good enough.The impacts of transmission error between two users for the whole systemBER performance were also investigated. 展开更多
关键词 differential space-time modulation decode-and-forward (DF) orthogonal design cooperative diversity
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Optimization and Testing for PCR System of Rice by Orthogonal Design 被引量:8
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作者 程芳艳 李春光 +5 位作者 刘永巍 孟巧霞 张景龙 宋冬明 刘华招 孟昭河 《Agricultural Science & Technology》 CAS 2010年第2期61-64,共4页
[Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves ... [Objective] This study was to screen the economic or stable PCR system of rice and detect the generality of the selected system in different molecular markers based on PCR.[Method] With DNA extracted from rice leaves by CTAB method as the template,PCR system was optimized by L16(45)orthogonal design.[Result] Clear bands were amplified from 16 different combinations,but the amplification effects and yields had difference.The most economic and applicable system was as follows:20 ng DNA template,150 μmol/L dNT... 展开更多
关键词 RICE PCR system optimization orthogonal design Dominant molecular markers testing
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Optimization of SRAP-PCR System of Triticum aestivum——Based on Orthogonal Design 被引量:2
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作者 艾鹏飞 武玉芬 魏景芳 《Agricultural Science & Technology》 CAS 2011年第3期366-368,374,共4页
[Objective] The aim was to optimize the technical procedure of SRAP-PCR in Triticum aestivum L. [Method] The orthogonal design L16(45) was used to optimize SRAP-PCR amplification system of wheat Fengyou 68 on five f... [Objective] The aim was to optimize the technical procedure of SRAP-PCR in Triticum aestivum L. [Method] The orthogonal design L16(45) was used to optimize SRAP-PCR amplification system of wheat Fengyou 68 on five factors (Taq polymerase,Mg2+,DNA template,dNTPs and primer) at four levels. [Result] Effects of the five factors on SRAP-PCR reaction system were Mg2+Taq polymerasedNTPsDNA templateprimer. Finally,an optimal SRAP-PCR system was established,that was the total 20 μl reaction system containing 2.0 μl 10×PCR Buffer,2.0 U Taq polymerase,2.0 mmol/L Mg2+,0.2 mmol/L dNTPs,40 ng DNA template and 0.6 μmol/L primer. [Conclusion] The optimum SRAP-PCR system had provided some technical foundations to conduct SRAP genetic analysis for wheat varieties. 展开更多
关键词 WHEAT SRAP orthogonal design OPTIMIZATION
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Study on Effects of Different Factors to Eggs Hatching Rate of Cryptotympana atrata by Orthogonal Design
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作者 孙会忠 宋月芹 +1 位作者 董钧锋 纠敏 《Agricultural Science & Technology》 CAS 2011年第4期551-553,共3页
[Objective] The paper aimed to explore influence of different factors on eggs hatching of Cryptotympana atrata,and optimize technical parameters in practice.[Method] By using hatching rate as index,L16(44)orthogonal... [Objective] The paper aimed to explore influence of different factors on eggs hatching of Cryptotympana atrata,and optimize technical parameters in practice.[Method] By using hatching rate as index,L16(44)orthogonal design was employed to determine the effects of the four factors including temperature,salinity,photoperiod and pH.[Result] Photoperiod had obviously effects on hatchability of Cryptotympana atrata eggs.The optimal conditions for hatching were temperature 30 ℃,salinity 25%,photoperiod L12∶D12,and pH 6.[Conclusion]The research provided some scientific basis for indoor artificial incubation. 展开更多
关键词 Cryptotympana atrata EGGS HATCHABILITY orthogonal design
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Optimization of RAPD-PCR Reaction System for Glycyrrhiza uralensis Based on Orthogonal Design 被引量:5
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作者 张增福 董建力 李明 《Agricultural Science & Technology》 CAS 2011年第10期1427-1429,共3页
[Objective] The aim was to obtain the optimum RAPD-PCR reaction system for Glycyrrhiza uralensis.[Method] Orthogonal design was adopted to screen the suitable concentration of four major factors(dNTPs,primers,Taq pol... [Objective] The aim was to obtain the optimum RAPD-PCR reaction system for Glycyrrhiza uralensis.[Method] Orthogonal design was adopted to screen the suitable concentration of four major factors(dNTPs,primers,Taq polymerase and DNA template) in PCR reaction system.[Result] The optimal reaction system obtained by orthogonal design was 25 μl in total volume,containing 2.5 μl of 10×PCR buffer solution(include MgCl2),2.5 μl of 10 mmol/L dNTPs,2 μl(100 ng) of DNA template,2 μl of 10 μmol/L primers,0.4 μl(5 U) of Taq polymerase;the optimum annealing temperature was 34 ℃.[Conclusion] Orthogonal design was an effective method for the optimization of RAPD-PCR reaction system for G.uralensis. 展开更多
关键词 Glycyrrhiza uralensis DNA RAPD-PCR Reaction system OPTIMIZATION orthogonal design
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Orthogonal design for optimization of pigment extraction from surface sediments of the Changjiang River Estuary 被引量:7
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作者 ZHAO Jun YAO Peng +1 位作者 YU Zhigang Thomas S Bianchi 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2011年第4期33-42,共10页
Using a suitable solvent for extracting pigments from sediment for high performance liquid chromatography(HPLC) analysis is critical for obtaining qualitative and quantitative estimates of phytoplanktonic and benthi... Using a suitable solvent for extracting pigments from sediment for high performance liquid chromatography(HPLC) analysis is critical for obtaining qualitative and quantitative estimates of phytoplanktonic and benthic algal biomass,as well as community composition. Five methodological factors(sample dehydration,extraction solvent,extraction duration,number of extractions,and ratio of solvent volume:sample weight) were studied using an L 9(3 4 ) orthogonal design in a sedimentary pigment extraction experiment on samples collected from the Changjiang large-river delta-front estuary(LDE),using HPLC analysis. The results show that the optimal extraction method for sedimentary pigments should include freeze-drying samples prior to extraction. The effects of different factors on sedimentary pigment extraction were separated by the L 9(3 4 ) orthogonal design experiments and showed that the extraction solvent was the most important,with extraction duration the second most important,and numbers of extraction and ratio of solvent volume:sample weight was the least important. The mixed solvent treatment comprised of acetone,methanol and water(80:15:5,by volume) was best for polar pigment extraction,with 100% acetone better for apolar pigments. For most pigments employed in this study(i.e.,peridinin,fucoxanthin,alloxanthin,diatoxanthin,zeaxanthin,pheophytin-a and β-carotene) ,3 h was found to be enough time for extraction from these deltaic sediments. However,for chlorophyll-a,the most important pigment used for estimating algal biomass,12 h was needed. A small amount of solvent(3 ml) with duplicate extractions obtained the greatest amount and diversity of pigments. Unfortunately,no extraction method was found to be suitable for all pigments in sediments. The choice of extraction procedure should be made in accordance with the objective of each study,taking into consideration the properties of sediments and pigments in question. 展开更多
关键词 sedimentary pigments EXTRACTION orthogonal design HPLC Changjiang River Estuary
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