Oscillating field stimulation promotes neurogenesis of neural stem cells through miR-124/Tal1 axis to repair spinal cord injury in rats

Spinal cord injury often leads to severe motor and sensory deficits,and prognosis using the currently available therapies remains poor.Therefore,we aimed to explore a novel therapeutic approach for improving the prognosis of spinal cord injury.In this study,we implanted oscillating field stimulation devices and transplanted neural stem cells into the thoracic region(T9–T10)of rats with a spinal cord contusion.Basso-Beattie-Bresnahan scoring revealed that oscillating field stimulation combined with neural stem cells transplantation promoted motor function recovery following spinal cord injury.In addition,we investigated the regulation of oscillating field stimulation on the miR-124/Tal1 axis in neural stem cells.Transfection of lentivirus was performed to investigate the role of Tal1 in neurogenesis of neural stem cells induced by oscillating field stimulation.Quantitative reverse transcription-polymerase chain reaction,immunofluorescence and western blotting showed that oscillating field stimulation promoted neurogenesis of neural stem cells in vitro and in vivo.Hematoxylin and eosin staining showed that oscillating field stimulation combined with neural stem cells transplantation alleviated cavities formation after spinal cord injury.Taking the results together,we concluded that oscillating field stimulation decreased miR-124 expression and increased Tal1 content,thereby promoting the neurogenesis of neural stem cells.The combination of oscillating field stimulation and neural stem cells transplantation improved neurogenesis,and thereby promoted structural and functional recovery after spinal cord injury.

Nucleation of Kinetic Ising Model Under Oscillating Field

We have studied the nucleation process of a two-dimensional kinetic Ising model subject to a bias oscillating external field, focusing on how the nucleation time depends on the oscillation frequency. It is found that the nucleation time shows a clear-cut minimum with the variation of oscillation frequency, wherein the average size of the critical nuclei is the smallest, indicating that an oscillating external field with an optimal frequency can be much more favorable to the nucleation process than a constant field. We have also investigated the effect of the initial phase of the external field, which helps to illustrate the occurrence of such an interesting finding.

Oscillating field stimulation promotes axon regeneration and locomotor recovery after spinal cord injury

Oscillating field stimulation(OFS)is a potential method for treating spinal cord injury.Although it has been used in spinal cord injury(SCI)therapy in basic and clinical studies,its underlying mechanism and the correlation between its duration and nerve injury repair remain poorly understood.In this study,we established rat models of spinal cord contusion at T10 and then administered 12 weeks of OFS.The results revealed that effectively promotes the recovery of motor function required continuous OFS for more than 6 weeks.The underlying mechanism may be related to the effects of OFS on promoting axon regeneration,inhibiting astrocyte proliferation,and improving the linear arrangement of astrocytes.This study was approved by the Animal Experiments and Experimental Animal Welfare Committee of Capital Medical University(supplemental approval No.AEEI-2021-204)on July 26,2021.

Epidural oscillating field stimulation increases axonal regenerative capacity and myelination after spinal cord trauma

Oscillating field stimulation(OFS)with regular alterations in the polarity of electric current is a unique,experimental approach to stimulate,support,and potentially guide the outgrowth of both sensory and motor nerve fibers after spinal cord injury(SCI).In previous experiments,we demonstrated the beneficial effects of OFS in a 4-week survival period after SCI.In this study,we observed the major behavioral,morphological,and protein changes in rats after 15 minutes of T9 spinal compression with a 40 g force,followed by long-lasting OFS(50μA),over a 8-week survival period.Three groups of rats were analyzed:rats after T9 spinal compression(SCI group);SCI rats subjected to implantation of active oscillating field stimulator(OFS+SCI group);and SCI rats subjected to nonfunctional OFS(nOFS+SCI group).Histopathological analysis of spinal tissue indicated a strong impact of epidural OFS on the reduction of tissue and myelin loss after SCI in the segments adjacent to the lesion site.Quantitative fluorescent analysis of the most affected areas of spinal cord tissue revealed a higher number of spared axons and oligodendrocytes of rats in the OFS+SCI group,compared with rats in the SCI and nOFS+SCI groups.The protein levels of neurofilaments(NF-l),growth-associated protein-43(marker for newly sprouted axons),and myelin basic protein in rats were signifiantly increased in the OFS+SCI group than in the nOFS+SCI and SCI groups.This suggests a supporting role of the OFS in axonal and myelin regeneration after SCI.Moreover,rats in the OFS+SCI group showed great improvements in sensory and motor functions than did rats in the nOFS+SCI and SCI groups.All these findings suggest that long-lasting OFS applied immediately after SCI can provide a good microenviroment for recovery of damaged spinal tissue by triggering regenreative processes in the acute phase of injury.

Molecular modeling of oscillating GHz electric field influence on the kinesin affinity to microtubule

Kinesin is a microtubule-associated motor protein which can respond to the external electric field due to its polarity.Using a molecular dynamics simulation method, the effect of such a field on the affinity of kinesin to the α β-tubulin is investigated in this study. To consider kinesin affinity, the system is exposed to an electric field of 0.03 V/nm with frequency values of 1, 2,..., 9, and 10 GHz. It is found that the applied electric field can change kinesin affinity to the microtubule.These changes could perturb the normal operation of kinesin, such as the processive motility of kinesin on the microtubule.

Photodetachment dynamics of H^- ion in a harmonic potential plus a time-dependent oscillating electric field

The photodetachment dynamics of H^- ion in a harmonic potential plus an oscillating electric field is studied using the time-dependent closed orbit theory. An analytical formula for calculating the photodetachment cross section of this system is put forward. It is found that the photodetachment cross section of this system is nearly unaffected for the weak oscillating electric field strength, but oscillates complicatedly when the oscillating electric field strength turns strong. In addition, the frequency of the harmonic potential and the oscillating electric field （the frequency of the harmonic potential and the frequency of the oscillating electric field are the same in the paper, unless otherwise stated.） can also affect the photodetachment dynamics of this system. With the increase of the frequency in the harmonic potential and the oscillating electric field, the number of the closed orbits for the detached electrons increased, which makes the oscillatory structure in the photodetachment cross section much more complex. Our study presents an intuitive understanding of the photodetachment dynamics driven by a harmonic potential plus an oscillating electric field from a space and time dependent viewpoint. This study is very useful in guiding the future experimental research for the photodetachment dynamics in the electric field both changing with space and time.

Temporal interference driven by an oscillating electric field in photodetachment of H^- ion

The real time domain interferometry for the photodetachment dynamics driven by the oscillating electric field has been studied for the first time. Both the geometry of the detached electron trajectories and the electron probability density are shown to be different from those in the photodetachment dynamics in a static electric field. The influence of the oscillating electric field on the detached electron leads to a surprisingly intricate shape of the electron waves, and multiple interfering trajectories generate complex interference patterns in the electron probability density. Using the semiclassical open-orbit theory, we calculate the interference patterns in the time-dependent electron probability density for different electric field strengths, different frequencies and phases in the oscillating electric field. This method is universal, and can be extended to study the photoionization dynamics of the atoms in the time-dependent electric field. Our study can guide the future experimental researches in the photodetachment or photoionization microscopy of negative ions and atoms in the oscillating electric field.

The Whole Theory of This Universe—A Step Forward to Einstein, Part-3rd: “The Universal Theory of Visible and Invisible Universe”

We believe that the universe is of two types: visible and invisible. Nothing is at rest between the invisible and visible universes. All microscopic bodies as well as all macroscopic bodies are in motion along curved paths (i.e. in circles). The universe inside an atom is as vast as the visible universe. An atom consists of millions of particles or particle galaxies which contain central energy pools or central energy cores. Energy pools present in the centre of the invisible universe inside atomic or subatomic particles from which particles and energy are continuously interconverting. In a dense central energy pool, two opposite charges are created due to the swirling motion of microscopic energy droplets. Small microscopic energy droplets may swirl either clockwise or anti-clockwise to produce microscopic tornadoes which are non-superimposable mirror images of each other and gain the property of positive and negative charges. Hence, the electrostatic force is originated between these two opposite charges, which are then changed into a pair of particles, i.e. catitron, which carries a positive charge, and anitron which carries a negative charge. All the other millions of subatomic particles or particle galaxies are produced in the same way. So, the electrostatic force is the basic force, and all other forces originate from this basic electrostatic force of attraction. When charged particles move, they produce an oscillating electric field, and the spinning of these particles produces oscillating magnetic fields. These oscillating electric and magnetic fields are perpendicular to each other and, by their interaction, an oscillating gravitational field is produced which is also perpendicular to both the oscillating electric field and the oscillating magnetic field. The Earth’s axial tilt, which causes the Earth’s precessional motion, is caused by the parallel alignment of the Earth’s magnetic field with the magnetic field of the Sun. Gravity is not a cause of space-time curvature, but gravity causes space-time curvature. Space-time curvature is nothing but a curved path around a heavy object. The Universal Theory of Visible and Invisible Universe—The Whole Theory of This Universe—A Step Forward to Einstein, opens new windows in the challenging fields of science and research, i.e. visible and invisible universe, universe inside an atom, what is the stuff of the entire universe? What will happen at the end of this whole universe?

Long-Time Dynamical Behaviour of Alternative-Current Stark Effect

We report the long time dynamical behaviour of ac Stark effect in a simple quantum model in which two level atoms interact with quantized coherent radiation field. A new phenomenon of periodic quantum collapse and revival of the ac Stark shift of energy level due to ac Stark effect is expressed accurately by analysing the phase of transition probability amplitude. The analytic prediction is confirmed by the numerical results.

MHD Seismology of a loop-like filament tube by observed kink waves

We report and analyze observational evidence of global kink oscillations in a solar filament as observed in Ha by instruments administered by National Solar Observatory （NSO）/Global Oscillation Network Group （GONG）. An M1.1-class flare in active region （AR） 11692 occurred on 2013 March 15 and induced a global kink mode in the filament lying towards the southwest of AR 11692. We find periods of about 61-67 minutes and damping times of 92-117 minutes at positions of three ver- tical slices chosen in and around the filament apex. We find that the waves are damped. From the observed period of the global kink mode and damping timescale using the theory of resonant absorption, we perform prominence seismology. We estimate a lower cut-off value for the inhomogeneity length scale to be around 0.34-0.44 times the radius of the filament cross-section.

Numerical simulations for MHD coronal seismology

Magnetohydrodynamic（MHD） processes are important for the transfer of energy over large scales in plasmas and so are essential to understanding most forms of dynamical activity in the solar atmosphere. The introduction of transverse structuring into models for the corona modifies the behavior of MHD waves through processes such as dispersion and mode coupling. Exploiting our understanding of MHD waves with the diagnostic tool of coronal seismology relies upon the development of sufficiently detailed models to account for all the features in observations. The development of realistic models appropriate for highly structured and dynamical plasmas is often beyond the domain of simple mathematical analysis and so numerical methods are employed. This paper reviews recent numerical results for seismology of the solar corona using MHD.

Analysis of DC and analog/RF performance on Cyl-GAA-TFET using distinct device geometry

In this paper, analysis of DC and analog/RF performance on cylindrical gate-all-around tunnel fieldeffect transistor（TFET） has been made using distinct device geometry. Firstly, performance parameters of GAATFET are analyzed in terms of drain current, gate capacitances, transconductance, source-drain conductance at different radii and channel length. Furthermore, we also produce the geometrical analysis towards the optimized investigation of radio frequency parameters like cut-off frequency, maximum oscillation frequency and gain bandwidth product using a 3D technology computer-aided design ATLAS. Due to band-to-band tunneling based current mechanism unlike MOSFET, gate-bias dependence values as primary parameters of TFET differ. We also analyze that the maximum current occurs when radii of Si is around 8 nm due to high gate controllability over channel with reduced fringing effects and also there is no change in the current of TFET on varying its length from 100 to 40 nm.However current starts to increase when channel length is further reduced for 40 to 30 nm. Both of these trades-offs affect the RF performance of the device.